丁苯酞联合依达拉奉治疗脑梗死急性期老年患者的疗效分析

目的探讨丁苯酞联合依达拉奉对脑梗死急性期老年患者脑血流动力学、血管斑块稳定性及血清同型半胱氨酸(Hcy)、超敏C反应蛋白(hs-CRP)水平的影响。方法选取2018年5月—2019年12月永康市第一人民医院收治的脑梗死急性期老年患者82例,采用随机数字表法将患者分为观察组和对照组,每组41例。观察组给予丁苯酞联合依达拉奉治疗;对照组给予依达拉奉治疗,均治疗2周。比较两组治疗2周总有效率;治疗前与治疗2周日常生活能力(ADL)评分和美国国立研究院卒中量表(NIHSS)评分、脑血流动力学、血管斑块稳定性、Hcy和hs-CRP水平的变化。结果观察组总有效率高于对照组(P<0.05)。观察组治疗前后ADL评分和NIHSS评分的差值大于对照组(P<0.05)。观察组治疗前后峰流速、平均流速的差值大于对照组(P<0.05)。两组治疗后稳定斑块比例高于治疗前(P<0.05)。治疗前,两组稳定斑块比例比较,差异无统计学意义(P>0.05);治疗2周后,观察组稳定斑块比例高于对照组(P<0.05)。观察组治疗前后血清Hcy、hs-CRP的差值大于对照组(P<0.05)。结论丁苯酞联合依达拉奉治疗脑梗死急性期老年患者疗效良好,且可改善患者脑血流动力学和血管斑块稳定性,降低血清Hcy、hs-CRP水平。

The application of EPICS in TMSR radiation protection and access control system

To treat technical problems of data acquisition,alarm management, and historical data archiving of radiation protection control system in Thorium Molten Salt Reactor, a network prototype system was designed based on experimental physics and industrial control system.Radiation level of many locations in Jiading campus of Shanghai Institute of Applied Physics is being continuously monitored, and boundaries of accelerator facilities and radiochemical areas are defined by the access control system. In this paper, we introduce the control system design,including human–machine interfaces, alarm system, historical data archiving system, and the software for access control. The software development followed the standard of Capability Maturity Model Integration(CMMIò) Level3, and the software had passed a third-party test, which indicated that the functionality and the reliability could fulfill the requirements of the radiation protection system.

Total flavone of Abelmoschus manihot suppresses epithelial-mesenchymal transition via interfering transforming growth factor-β1 signaling in Crohn's disease intestinal fibrosis

AIM To explore the role and mechanism of total flavone of Abelmoschus manihot(TFA) on epithelial-mesenchymal transition(EMT) progress of Crohn's disease(CD) intestinal fibrosis.METHODS First,CCK-8 assay was performed to assess TFA on the viability of intestinal epithelial(IEC-6) cells and select the optimal concentrations of TFA for our further studies.Then cell morphology,wound healing and transwell assays were performed to examine the effect of TFA on morphology,migration and invasion of IEC-6 cells treated with TGF-β1.In addition,immunofluorescence,real-time PCR analysis(q RT-PCR) and western blotting assays were carried out to detect the impact of TFA on EMT progress.Moreover,western blotting assay was performed to evaluate the function of TFA on the Smad and MAPK signaling pathways.Further,the role of co-treatment of TFA and si-Smad or MAPK inhibitors has been examined by q RTPCR,western blotting,morphology,wound healing andtranswell assays.RESULTS In this study,TFA promoted transforming growth factor-β1(TGF-β1)-induced(IEC-6) morphological change,migration and invasion,and increased the expression of epithelial markers and reduced the levels of mesenchymal markers,along with the inactivation of Smad and MAPK signaling pathways.Moreover,we revealed that si-Smad and MAPK inhibitors effectively attenuated TGF-β1-induced EMT in IEC-6 cells.Importantly,co-treatment of TFA and si-Smad or MAPK inhibitors had better inhibitory effects on TGF-β1-induced EMT in IEC-6 cells than either one of them.CONCLUSION These findings could provide new insight into the molecular mechanisms of TFA on TGF-β1-induced EMT in IEC-6 cells and TFA is expected to advance as a new therapy to treat CD intestinal fibrosis.

Non-SMC condensin Ⅰ complex subunit D2 and non-SMC condensin Ⅱ complex subunit D3 induces inflammation via the IKK/NF-κB pathway in ulcerative colitis

BACKGROUND Ulcerative colitis(UC)is a chronic,nonspecific intestinal inflammatory disease with undefined pathogenesis.Non-SMC condensin I complex subunit D2(NCAPD2)and non-SMC condensin II complex subunit D3(NCAPD3)play pivotal roles in chromosome assembly and segregation during both mitosis and meiosis.To date,there has been no relevant report about the functional role of NCAPD2 and NCAPD3 in UC.AIM To determine the level of NCAPD2/3 in intestinal mucosa and explore the mechanisms of NCAPD2/3 in UC.METHODS Levels of NCAPD2/3 in intestinal tissue were detected in 30 UC patients and 30 healthy individuals with in situ hybridization(ISH).In vitro,NCM60 cells were divided into the NC group,model group,si-NCAPD2 group,si-NCAPD3 group and si-NCAPD2+si-NCAPD3 group.Inflammatory cytokines were measured by ELISA,IKK and NF-κB were evaluated by western blot,and IKK nucleation and NF-κB volume were analyzed by immunofluorescence assay.RESULTS Compared with expression in healthy individuals,NCAPD2 and NCAPD3 expression in intestinal tissue was significantly upregulated(P<0.001)in UC patients.Compared with levels in the model group,IL-1β,IL-6 and TNF-αin the si-NCAPD2,si-NCAPD3 and si-NCAPD2+si-NCAPD3 groups were significantly downregulated(P<0.01).IKK and NF-κB protein expression in the si-NCAPD2,si-NCAPD3 and si-NCAPD2+si-NCAPD3 groups was significantly decreased(P<0.01).Moreover,IKK nucleation and NF-κB volume were suppressed upon si-NCAPD2,si-NCAPD3 and si-NCAPD2+si-NCAPD3 transfection.CONCLUSION NCAPD2/3 is highly expressed in the intestinal mucosa of patients with active UC.Overexpression of NCAPD2/3 promotes the release of pro-inflammatory cytokines by modulating the IKK/NF-κB signaling pathway.

The deposition of asphaltenes under high-temperature and highpressure (HTHP) conditions

In this work,the factors affecting asphaltenes deposition in high-temperature and high-pressure wells were studied using backscattered light and PVT equipment customized to suit the well conditions.In an examination of the intensity of backscattered light,it was revealed that there exists a linear relationship between temperature and asphaltene precipitation within a specific temperature range.Within this range,a decrease in temperature tends to accelerate asphaltene precipitation.However,the impacts of pressure and gas-oil ratio are more pronounced.The pressure depletion induces the asphaltenes to precipitate out of the solution,followed by the formation of flocs below the bubble point.In addition,an increase in the gas-oil ratio causes a more severe asphaltene deposition,shifting the location of asphaltenes to deep well sections.

Association of hidradenitis suppurativa with Crohn’s disease

Hidradenitis suppurativa(HS)is a chronic inflammatory skin disorder characterized by recurrent nodules,abscesses,and sinus tracts.Crohn’s disease(CD)is characterized by inflammation of the entire digestive tract and belongs to the group of inflammatory bowel diseases,and there are many extraintestinal manifestations,among which hidradenitis suppurativa is one of the rare extraintestinal manifestations.There appears to be a strong association between CD and HS based on clinical and histological similarities(sinus tract development,granulomatous inflammation,and scarring),intersections in pathogenesis(genetic loci,immune dysregulation mechanisms,and microbiome changes),and commonality in treatment.In this review,we summarize recent studies on the association between HS and CD.

Role of tumor associated macrophages in relapse of primary gastrointestinal diffuse large B cell lymphoma and their clinical significance

Objective:To investigate the correlation between the change in expression of the tumor associated macrophage (TAM) markers CD68 and CD206 and the clinicopathological parameters.Methods: We retrospectively analyzed 28 PGI-DLBCL patients by immunohistochemical stainning. The surface expression of biomarkers CD68 and CD206 on TAMs in samples obtained at diagnosis was compared with that in specimens obtained at first relapse. Based on the change in expression levels of CD68 and CD206 from diagnosis to relapse, patients were categorized into three groups:'increased','stable' and'decreased'. Results: A significant difference was observed between the three groups with respect to CD68 and CD206 expressions (P=0.012) and there existed a consistency between the two (P=0.008). The three groups showed a negative correlation with age, sex, stage, B symptoms, diagnosis, ECOG performance status, and IPI scores, while a positive correlation was observed with the site of lesion of CD206 expression (P=0.032). The time to relapse (TTR) in the three groups of CD68 and CD206 was not significantly different (P=0.23,P=0.818).Conclusion: Our study suggests that increase in CD206 and decrease in CD68 expression at relapse indicates M1-TAM polarization into M2-TAM, which plays an important role in PGI-DLBCL relapse. Furthermore, M2-TAM polarization may be associated with the site of Primary gastric diffuse large B lymphoma.

Antibacterial mechanism of taxifolin and its application in milk preservation

Taxifolin is a flavonoid commonly found in fruits and vegetables with a variety of bioactivities.However,it is unknown whether taxifolin has positive effects on food preservation.In the present study,the antibacterial activity and mechanism of taxifolin on two foodborne bacteria,Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus),were investigated for the first time.Besides,its applications in milk preservation were evaluated.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of taxifolin on E.coli were 1.11 mg/mL and 2.22 mg/mL,while for S.aureus were 0.556 mg/mL and 1.11 mg/mL,respectively.Furthermore,taxifolin exhibited strong synergistic antibacterial activity with tetracycline.Taxifolin functions against the two strains of bacteria through damaging bacterial cell wall and membrane integrity,inhibiting bacterial biofilm formation,causing adversity and resulting in the increase of superoxide dismutase and alkaline phosphatase activities in bacteria.Moreover,morphological changes in bacteria were observed after exposure to taxifolin,including the formation of aggregates,irregular forms,wrinkled cell surfaces,and shriveling of cells.During milk storage,taxifolin significantly delayed the increase of acidity and the loss of lactose in milk through inhibiting the proliferation of bacteria.Hence,in addition to health-promoting bioactivities,taxifolin also has positive effects on food preservation because of antibacterial activity.