Influence of initial check, information exchange, final accuracy check, reaction information nursing on the psychology of elderly with lung cancer

BACKGROUND As one of the fatal diseases with high incidence,lung cancer has seriously endangered public health and safety.Elderly patients usually have poor self-care and are more likely to show a series of psychological problems.AIM To investigate the effectiveness of the initial check,information exchange,final accuracy check,reaction(IIFAR)information care model on the mental health status of elderly patients with lung cancer.METHODS This study is a single-centre study.We randomly recruited 60 elderly patients with lung cancer who attended our hospital from January 2021 to January 2022.These elderly patients with lung cancer were randomly divided into two groups,with the control group taking the conventional propaganda and education and the observation group taking the IIFAR information care model based on the conventional care protocol.The differences in psychological distress,anxiety and depression,life quality,fatigue,and the locus of control in psychology were compared between these two groups,and the causes of psychological distress were analyzed.RESULTS After the intervention,Distress Thermometer,Hospital Anxiety and Depression Scale(HADS)for anxiety and the HADS for depression,Revised Piper’s Fatigue Scale,and Chance Health Locus of Control scores were lower in the observation group compared to the pre-intervention period in the same group and were significantly lower in the observation group compared to those of the control group(P<0.05).After the intervention,Quality of Life Questionnaire Core 30(QLQ-C30),Internal Health Locus of Control,and Powerful Others Health Locus of Control scores were significantly higher in the observation and the control groups compared to the pre-intervention period in their same group,and QLQ-C30 scores were significantly higher in the observation group compared to those of the control group(P<0.05).CONCLUSION The IIFAR information care model can help elderly patients with lung cancer by reducing their anxiety and depression,psychological distress,and fatigue,improving their tendencies on the locus of control in psychology,and enhancing their life qualities.

Surviving winter on the Qinghai-Xizang Plateau:Extensive reversible protein phosphorylation plays a dominant role in regulating hypometabolism in hibernating Nanorana parkeri

Changes in protein abundance and reversible protein phosphorylation(RPP)play important roles in regulating hypometabolism but have never been documented in overwintering frogs at high altitudes.To test the hypothesis that protein abundance and phosphorylation change in response to winter hibernation,we conducted a comprehensive and quantitative proteomic and phosphoproteomic analysis of the liver of the Xizang plateau frog,Nanorana parkeri,living on the Qinghai-Xizang Plateau.In total,5170 proteins and 5695 phosphorylation sites in 1938 proteins were quantified.Based on proteomic analysis,674 differentially expressed proteins(438 up-regulated,236 down-regulated)were screened in hibernating N.parkeri versus summer individuals.Functional enrichment analysis revealed that higher expressed proteins in winter were significantly enriched in immune-related signaling pathways,whereas lower expressed proteins were mainly involved in metabolic processes.A total of 4251 modified sites(4147 up-regulated,104 down-regulated)belonging to 1638 phosphoproteins(1555 up-regulated,83 down-regulated)were significantly changed in the liver.During hibernation,RPP regulated a diverse array of proteins involved in multiple functions,including metabolic enzymatic activity,ion transport,protein turnover,signal transduction,and alternative splicing.These changes contribute to enhancing protection,suppressing energy-consuming processes,and inducing metabolic depression.Moreover,the activities of phosphofructokinase,glutamate dehydrogenase,and ATPase were all significantly lower in winter compared to summer.In conclusion,our results support the hypothesis and demonstrate the importance of RPP as a regulatory mechanism when animals transition into a hypometabolic state.

Neuroprotective effects of insulin-like growth factor-2 in 6-hydroxydopamine-induced cellular and mouse models of Parkinson’s disease

Skin-derived precursor Schwann cells have been reported to play a protective role in the central nervous system. The neuroprotective effects of skin-derived precursor Schwann cells may be attributable to the release of growth factors that nourish host cells. In this study, we first established a cellular model of Parkinson’s disease using 6-hydroxydopamine. When SH-SY5 Y cells were pretreated with conditioned medium from skin-derived precursor Schwann cells, their activity was greatly increased. The addition of insulin-like growth factor-2 neutralizing antibody markedly attenuated the neuroprotective effects of skin-derived precursor Schwann cells. We also found that insulin-like growth factor-2 levels in the peripheral blood were greatly increased in patients with Parkinson’s disease and in a mouse model of Parkinson’s disease. Next, we pretreated cell models of Parkinson’s disease with insulin-like growth factor-2 and administered insulin-like growth factor-2 intranasally to a mouse model of Parkinson’s disease induced by 6-hydroxydopamine and found that the level of tyrosine hydroxylase, a marker of dopamine neurons, was markedly restored, α-synuclein aggregation decreased, and insulin-like growth factor-2 receptor downregulation was alleviated. Finally, in vitro experiments showed that insulin-like growth factor-2 activated the phosphatidylinositol 3 kinase(PI3 K)/AKT pathway. These findings suggest that the neuroprotective effects of skin-derived precursor Schwann cells on the central nervous system were achieved through insulinlike growth factor-2, and that insulin-like growth factor-2 may play a neuroprotective role through the insulin-like growth factor-2 receptor/PI3 K/AKT pathway. Therefore, insulin-like growth factor-2 may be an useful target for Parkinson’s disease treatment.

两种检测HAV-IgM试剂的敏感性评价

目的对国内市场上应用较广的两种甲型肝炎病毒抗体(HAV-Ig M)检测试剂进行敏感性评价。方法收集疑似HAV感染者的血清标本,以美国雅培公司的HAV-Ig M试剂检测结果作为金标准,评价北京万泰生物药业股份有限公司和北京现代高达生物技术有限责任公司两种检测HAV-Ig M试剂的敏感性;比较两种HAV-Ig M试剂样本值/临界值(S/CO)的相关性;评价两种检测HAV-Ig M试剂的一致性。结果万泰HAV-Ig M检测试剂和高达HAV-Ig M检测试剂的敏感性分别为94.3%、97.1%,特异性分别为85.2%、91.3%;万泰、高达与雅培HAV-Ig M检测试剂S/CO值有相关(r=0.597和0.541,均P=0.000);万泰、高达与雅培HAV-Ig M检测试剂结果均高度一致(K=0.648和0.772,均P=0.000)。结论万泰、高达试剂均有很好的检测性能,为临床检测和人群血清流行病学调查参考试剂选择提供重要参考依据。

一种HBV DNA定量检测试剂的准确性分析

目的评价一种新型HBV DNA定量检测试剂的准确性。方法将第3代HBV DNA WHO国际标准品稀释为6种不同梯度浓度样本,8.5×10~5、8.5×10~4、8.5×10~3、8.5×10~2、85及42.5 IU/ml,用待评价试剂care HBV PCR Assay V3.0检测,并对实测值和理论值进行相关性分析;以Cobas Taqman HBV V2.0为参比试剂,用care HBV PCR Assay V3.0检测93份HBV感染者标本和30份健康者标本HBV DNA含量,并对2种试剂定量检测结果进行相关性和一致性分析;采用巢式PCR扩增漏检标本的HBV S区,直接测序法测定其基因型。结果 care HBV PCR Assay V3.0检测WHO国际标准品的实测值和理论值具有线性相关;2种定量试剂检测93份HBV感染者标本结果均阳性共68份,检测结果呈线性相关,差异无统计学意义(P>0.05),但care HBV PCR Assay V3.0检测值相对偏低;care HBV PCR Assay V3.0检测有14份标本漏检,其中13份标本Cobas Taqman HBV V2.0检测结果位于30~2 000 IU/ml间;对1份漏检标本的基因型检测结果为C型。结论 care HBV PCR Assay V3.0与Cobas Taqman HBV V2.0试剂检测结果有较好的相关性,但对基因型C型的标本可能存在漏检现象。

Advanced Space-based Solar Observatory(ASO-S):an overview

The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C.

Design of the Full-disk Magneto Graph (FMG) onboard the ASO-S

The Full-disk Magneto Graph(FMG),a payload onboard the Advanced Space Solar Observatory(ASO-S),will measure the vector magnetic field in the photosphere.The instrument consists of a frontwindow filter,a telescope,an LCVR polarimeter,an image-stabilization system,a seven-stage tunable Lyot filter,a CMOS camera with 4096×4096 pixels and a pair of calibration/focus wheels.In this paper,we describe the design of the FMG instrument and provide a summary of test observations carried out with the FMG prototype.

Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease

Autophagy has been shown to play an important role in Parkinson’s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells(SKP-SCs).The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine,reduced excessive autophagy,increased tyrosine hydroxylase expression,reducedα-synuclein expression,reduced the autophagosome number,and activated the PI3K/AKT/mTOR pathway.Autophagy activator rapamycin inhibited the effects of SKP-SCs,and autophagy inhibitor 3-methyladenine had the opposite effect.These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy,thereby exhibiting a neuroprotective effect in a cellular model of Parkinson’s disease.This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University(approval No.S20181009-205)on October 9,2018.

srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons by inactivating RAC1

Objective:To explore effect of srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons.Methods:In this study,expression of Slit1 was observed predominantly in the glia.while expression of Robo2 and srCAP3 was detected in sensory neurons of postnatal rat cultured dorsal root ganglion(DRG).Furthermore,upregulation of srGAP3 following sciatic nerve transection was detected by immunohistochemistry and Western blotting.Results:It was observed that inhibition of nenrite outgrowth in cultured adult DRG neurons following treatment with anti-srGAP3 or anti-Robo2 was more effectively(1.5-fold higher) than that following treatment with an anti-BDNF positive control antibody.It demonstrated that srGAP3 interacted with Robo2 and Slit1 protein to decrease Rac1-CTP activity in cultured adult rat DRG neurons and the opposite effect on Rac1-GTP activity was detected by co-immunoprecipitation and immunoblotting analyses following treatment with anti-Robo2 or anti-srGAP3.These data demonstrated a role for srGAP3 in nenrite outgrowth ol DRG sensory neurons.Conclusions:Our observations suggest that srGAP3 promotes neurile outgrowth and filopodial growth cones by interacting with Robo2 to inactivate Rac1 in mammalian DRG neurons.

The protective effect and underlying mechanism of Hainan papaya water extract against neuronal apoptosis induced by A β 40

Objective:To investigate whether Hainan papayas has protective effects in an Aimary neuron injury model and elucidate the underlying molecular mecha β 40-induced prnism.Methods:Cultured primary neurons from the dorsal root ganglia(DRG) of Sprague-Dawley(SD) rats were treated with 20 μM A culture medium β 40 peptide,100 μg/L Hainan papaya water extract,peptide plus extract,or for 24 h.Cell viability was measured by MTT assay,and neuronal apoptosis was evaluated by DAPI staining.ERK signaling pathway-associated molecule activation and changes in Bax expression were analyzed by Western blotting and immunofluorescence.Results:A cell viability rate of(44.11±6.59)% in the Ading different concentrations of the extract β 40 group was rescued to(79.13±6.64)% by ad.DAPI showed pyknotic nuclei in 39.5% of A β 40-treated cells;the fraction dropped to 17.4% in the 100 μg/L extract group.ERK phosphorylation was observed in the Aent with 100 μg/L extract.Hainan papaya water ex β 40 group but was ameliorated by pretreatmtract also prevented Ation of MEK,RSK1 and CREB associated with ERK signaling and d β 40-induced phosphorylaownregulated Bax expression in the neurons.Conclusion:The resultss suggest that Hainan papaya water extract has protective effects on neurons;the mechanism may be related to suppression of ERK signaling activation.

Current status of stem cell therapy for Alzheimer's disease

Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research, researchers have attempted to compensate for neuronal loss in the brains of patients with Alzheimer's disease using stem cell technology. This article will review the pathogenesis of AD, the latest advances in stem cell therapy, and the challenges and prospects of its development.

SYNTHESIS OF NOVEL THIOPHENEDIMETHYLENE BRIDGED HOMOBINUCLEAR METALLOCENES AND THEIR CATALYTIC PROPERTIES FOR ETHYLENE POLYMERIZATION

By treating disodium（thiophenedimethylene）dicyclopentadienide C4H2S（CH2C5H4Na）2 with two equivalent of CpTiCl3 or CpZrC13 DME at 0℃ in THF, two new thiophenedimethylene bridged binuclear metallocenes [Cl2MC5H5][C5H4CH2C4H2SCH2C5H4][C5H5MCl2] （M = Ti 3, M = Zr 4） were synthesized in high yield and their structures were characterized by ^1H-NMR. These complexes were used as catalysts for ethylene polymerization in the presence of methylaluminoxane （MAO）. The effects of polymerization temperature, time, concentration of catalyst, molar ratio of MAO/Cat on polymerization were studied in detail. The catalytic activities of thiophenedimethylene bridged binuclear metallocene catalysts （3, 4） reached 2.44 × 10^5 g PE mol^-1 cat^-1· h^-1, 9.61 × 10^5 g PE mol^-1 · cat^-1· h^-1 respectively, which are higher than that of pheneyldimethylene bridged binuclear metallocene catalysts and much higher than that of corresponding mononuclear metallocenes （Cp2TiCl2 and Cp2ZrCl2）. The molecular weight distribution curves of polyethylenes produced by binuclear metallocene catalysts （3, 4） and by mononuclear metallocene catalyst have only single peak, but the former （MWD = 3.5-4.7） is obviously broader than the latter （MWD = 2.0-2.2）.

Tag single nucleotide polymorphism rs1532624 located in cholesteryl ester transfer protein gene is associated with atherosclerosis cerebral ischemia

Objective: To investigate the relationship between polymorphisms of rs1532624 and rs289741 loci in cholesteryl ester transfer protein(CETP) genes and atherosclerotic cerebral infarction(ACI). Methods: The CETP gene rs1532624 and rs289741 in 95 patients with ACI and 177 healthy subjects were genotyped by Mass ARRAY mass spectrometry. Each locus genotype and allele frequency distributions were compared. Results: The difference of allele frequency distribution between the rs1532624(χ~2=1.723, P=0.189) and rs289741(χ~2=2.466, P=0.116) were not statistically significant. The frequency distribution of rs1532624 genotype between the cerebral infarction group and healthy control group was statistically significant(χ~2=7.096, P=0.029), while rs289741 genotype frequency distribution between the two groups was not statistically significant(χ~2=2.906, P=0.234). Conclusion: ACI have a positive correlation with rs1532624 polymorphism, and AA genotype may be susceptible factors of ACI.

Application of adipose-derived stem cells in treatment of neurodegenerative diseases:A review

Neurodegenerative diseases are a class of progressive degenerative diseases that mainly include neuronal function decline and loss,such as Alzheimer's disease(AD);Cerebral troke;Parkinson's disease(PD);etc.These diseases are difficult to cure and seriously affect people's quality of life.Adipose derived stem cells(ADSCs)are derived from mesenchymal stem cells isolated and purified from adipose tissue.They have the characteristics of multidirectional differentiation and are widely used in the treatment of neurodegenerative diseases.Therefore,the use of ADSCs to treat this type of disease has potential application value.This article discusses the role of ADSCs in neurodegenerative diseases and their regeneration characteristics,and discusses the current clinical applications and future challenges of ADSCs,in order to provide references for the clinical application of ADSCs.

Effect and mechanism of dexmedetomidine on ischemic arrhythmia

Objective:To explore the antiarrhythmic effect and mechanism of dexmedetomidine,a myocardialα2 receptor agonist in vitro.Methods:Fifty Wistar rats were randomly divided into five groups(n=10):sham operation group(Ctl),arrhythmia model group(Model),arrhythmia+dexmedetomidine group(Dex),arrhythmia+yohimbine+dexmedetomidine group(Yoh+Dex),arrhythmia+yohimbine group(Yoh).The Ctl group just thread left anterior descending coronary artery without ligation.Model group recorded 10 minutes of normal ECG,ligated the left anterior descending coronary artery,and continued to record the ECG for 2 hours.Dex group,Yoh+Dex group and Yoh group were ligated left anterior descending coronary artery after administration.Use BL-420E system to record ECG;Curtis and Walker arrhythmia scores were used to analyze the severity of arrhythmia;perform survival analysis according to the life span of each group of rats;after 21 days of modeling,measure the area of myocardial infarction by TTC staining.The pH value of extracellular fluid was decreased to simulate myocardial ischemia.Patch clamp technique was used to detect the action potential duration of myocardial cells.Results:Compared with the Ctl group,the arrhythmia score and myocardial infarction area in the Model group was increased(P<0.05),the mortality and myocardial infarction area were alsosignificantly reduced(P<0.05).Compared with Model group and Yoh group,the arrhythmia score of Dex group was significantly lower(P<0.001),the mortality and myocardial infarction area were significantly lower(P<0.05);Dexmedetomidine shortened QTc interval(P<0.01),APD50 and APD90(P<0.05).The α_(2) receptor blocker Yohimbine inhibited the effect of dexmedetomidine.Conclusion:Dexmedetomidine affects the action potential repolarization process by stimulating myocardial α_(2) receptors,and prevents ischemia-induced ventricular arrhythmia.

Construction of a lentiviral vector over - expressing BDNF and its transfection into adipose derived stem cell lines in vitro

Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed and inserted into the lentiviral vector p HBLV-CMVIE-Zs Green-Puro. After identification by DNA sequencing, the lentiviral vectors carrying BDNF were packaged in 293 cells. The transfection efficiency was observed under fluorescence microscope;the lentiviral particles were collected to infect mouse ADSCs, and BDNF level were assessed using real-time PCR and Western blotting.Results: DNA sequencing demonstrated successful construction of the BDNF lentivirus vectors. Real- time PCR and Western blotting showed a high level BDNF mRNA and protein.Conclusion: We have successfully established an ADSC model with stable BDNF expression which establish the basis for the potential application of ADSCs-BDNF in the treatment of AD.

Advance of glial cells in Alzheimer's disease

Alzheimer's disease(AD)has an unknown pathogenesis,which is one of the key factors leading to the death of the elderly.Recent studies have shown that glial cells,including microgli、astrocytes、oligo-dendrocytes and oligodendrocyte progenitor cells(NG2 glial cells),are closely related to the pathogenesis of AD.It is believed that activated microglial cells are very easy to induce neuritis and then lead to neurodegeneration.In this paper,the characteristics of the above four glial cells,their functions and their correlation with AD were reviewed,in order to provide a reference for the further study of the pathogenesis of AD.

Dimensional regulation in gigantic molybdenum blue wheels featuring{(W)Mo_(5)}motifs for enhanced proton conductivity

Dimensional regulation in polyoxometalates is an effective strategy during the design and synthesis of polyoxometalates-based high proton conductors,but it is not available to date.Herein,the precise regulation of dimensionality has been realized in an unprecedented gigantic molybdenum blue wheel family featuring pentagonal{(W)Mo5}motifs through optimizing the molar ratio of Mo/W,including[Gd_(2)Mo_(124)W_(14)O_(422)(H_(2)O)62]38-(0D-{Mo_(124)W_(14)},1),[Mo_(126)W_(14)O441(H_(2)O)51]^(70-)(1D-{Mo_(126)W_(14)}n,2),and[Mo_(124)W_(14)O_(430)(H_(2)O)50]60-(2D-{Mo_(124)W_(14)}n,3).Such important{(W)Mo5}structural motif brings new reactivity into gigantic Mo blue wheels.There are different numbers and sites of{Mo2}defects in each wheel-shaped monomer in 1-3,which leads to the monomers of 2 and 3 to form 1D and 2D architectures via Mo-O-Mo covalent bonds driven by{Mo2}-mediated H_(2)O ligands substitution process,respectively,thus achieving the controllable dimensional regulation.As expected,the proton conductivity of 3 is 10 times higher than that of 1 and 1.7 times higher than that of 2.The continuous proton hopping sites in 2D network are responsible for the enhanced proton conductivity with lower activation energy.This study highlights that this dimensional regulation approach remains great potential in preparing polyoxometalates-based high proton conductive materials.