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基于ITS2的和田玫瑰分子进化分析
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作者 宁慧霞 鹿娟娟 +4 位作者 鲁春芳 文洪江 努尔波拉提·阿依达尔汗 海白尔·火加艾合买提 刘戈宇 《中国现代中药》 CAS 2022年第7期1225-1233,共9页
目的:分析和田玫瑰分子系统发育进化式样并评价其道地性。方法:采用聚合酶链式反应获得和田玫瑰内转录间隔区2(ITS2)序列,在美国国家生物技术信息中心数据库(NCBI)比对,从GenBank获取邻近属ITS2序列共52条,利用MEGAX软件进行多重比对分... 目的:分析和田玫瑰分子系统发育进化式样并评价其道地性。方法:采用聚合酶链式反应获得和田玫瑰内转录间隔区2(ITS2)序列,在美国国家生物技术信息中心数据库(NCBI)比对,从GenBank获取邻近属ITS2序列共52条,利用MEGAX软件进行多重比对分析,构建邻接(NJ)系统发育树,推断进化时间,预测ITS2序列二级结构。结果:基于ITS2引物扩增获得长度为409 bp的序列,包括小亚基核糖体核糖核酸基因和内部转录间隔区的部分序列(1~39 bp)、5.8S核糖体核糖核酸基因全序列(40~64 bp)、ITS2标准全序列(65~268 bp)、大亚基核糖体核糖核酸基因的部分序列(269~409 bp)。分析发现和田玫瑰与英国、意大利等欧洲产玫瑰Rosa sherardii距离最近,共同亲本是大马士革玫瑰,和田玫瑰于20000多年前开始出现。结论:和田玫瑰ITS2标准序列为204 bp,起源于欧洲大马士革玫瑰,分歧进化时间约为29000年,与《中华人民共和国药典》2020年版收录玫瑰花的ITS2序列二级结构的区别主要在Ⅲ螺旋区部位。 展开更多
关键词 和田玫瑰 道地性 内转录间隔区2 系统发育 进化时间
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家独行菜栽培适应性研究
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作者 宁慧霞 海白尔·火加艾合买提 刘戈宇 《现代中药研究与实践》 CAS 2020年第6期6-9,共4页
目的研究家独行菜引种栽培适应性。方法以药材市场上购买的家独行菜种子为研究材料,以采集的野生材料为对照进行栽培种植(株行距10 cm×35 cm),调查物候期、株高、丛幅(长×宽,cm^2)、主茎有效分枝数、抗逆性等农艺性状。结果... 目的研究家独行菜引种栽培适应性。方法以药材市场上购买的家独行菜种子为研究材料,以采集的野生材料为对照进行栽培种植(株行距10 cm×35 cm),调查物候期、株高、丛幅(长×宽,cm^2)、主茎有效分枝数、抗逆性等农艺性状。结果栽培型家独行菜株高45.8~60.4 cm,丛幅(长×宽,cm^2)200.2~368.7 cm^2,主茎有效分枝数5~9个;野生型株高20~40 cm,丛幅(长×宽,cm^2)98.2~238.5 cm^2,主茎有效分枝数3~7个。经统计分析,栽培型与野生型差别有统计学意义,栽培型家独行菜在株高、丛幅等农艺性状均显著高于野生型,且在引种栽培区域未发生病虫害。结论家独行菜在和田地区栽培有较好的适宜性及抗逆性。 展开更多
关键词 家独行菜 栽培 适应性 生长特性
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Study on Cultivation Techniques for Vernonia anthelmintica Willd
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作者 Nurbolat Aidarhan Abulimiti Yili +2 位作者 haibaier huojiaaihemaiti Chunfang LU Haji Akber Aisa 《Medicinal Plant》 CAS 2020年第4期64-65,共2页
This article focuses on the cultivation techniques and harvesting of Vernonia anthelmintica Willd.At present,the domestic cultivation of V.anthelmintica Willd has not formed a scale,characterized with low seed yield a... This article focuses on the cultivation techniques and harvesting of Vernonia anthelmintica Willd.At present,the domestic cultivation of V.anthelmintica Willd has not formed a scale,characterized with low seed yield and high harvesting cost.Therefore,it is of great significance to carry out research on the cultivation techniques and yield improvement of V.anthelmintica Willd. 展开更多
关键词 Vernonia anthelmintica Willd Medicinal value Chemical composition Pharmacological action Cultivation technique
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Effect of Sowing Rate,Fertilizer Amount and Light on Yield of Cichorum glandulosum Boiss. Et Huet.
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作者 Yanrui CHEN Juling WANG +4 位作者 Nurbolat Aidarhan Xu BAI Geyu LIU haibaier huojiaaihemaiti Abulimiti Yili 《Asian Agricultural Research》 2021年第3期57-59,共3页
[Objectives]This study aimed to investigate the effect of sowing rate,fertilizer amount and light on yield of Cichorium glandulosum Boiss.et Huet.[Methods]The Latin square design was adopted to investigate the effect ... [Objectives]This study aimed to investigate the effect of sowing rate,fertilizer amount and light on yield of Cichorium glandulosum Boiss.et Huet.[Methods]The Latin square design was adopted to investigate the effect of sowing rate+fertilizer amount,sowing rate+light on root and seed yield of C.glandulosum Boiss.et Huet.The changes in the seed and root yield of C.glandulosum Boiss.et Huet.under the influence of sowing rate,N application rate and light were compared and analyzed.[Results]In Moyu,Hotan and similar areas,the suitable sowing rate of C.glandulosum Boiss.et Huet.was 4.5-15.0 kg/ha.Without applying fertilizer,if the main purpose is to harvest the roots,the sowing rate of C.glandulosum Boiss.et Huet.is recommended to be 7.5-15.0 kg/ha;and if the main purpose is to harvest the seeds,the sowing rate is recommended to be 4.5-7.5 kg/ha.Shading will affect the root and seed yield.At the fertilizer amount of 450 kg/ha,the optimal sowing rate of C.glandulosum Boiss.et Huet.is 4.5 kg/ha.[Conclusions]This study provides a certain reference basis for artificial cultivation of C.glandulosum Boiss.et Huet. 展开更多
关键词 Cichorium glandulosum Boiss.et Huet. Sowing rate Nitrogen application amount LIGHT YIELD
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Study on Cultivation Technology of Rosa rugosa Thunb.
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作者 Nurbolat AIDARHAN Yanrui CHEN +2 位作者 haibaier huojiaaihemaiti Abulimiti YILI Haiji Akber AISA 《Agricultural Biotechnology》 CAS 2020年第3期44-46,共3页
Rosa rugosa Thunb. is a traditional medicinal and food plant in Hotan region,which has important medicinal and economic value. In recent years,the planting area in Hotan region has been expanding. This paper described... Rosa rugosa Thunb. is a traditional medicinal and food plant in Hotan region,which has important medicinal and economic value. In recent years,the planting area in Hotan region has been expanding. This paper described the cultivation techniques,planting models and field management,pest control,harvesting and processing of R. rugosa. Based on the analysis of the advantages and cultivation techniques of developing the rose industry in Hotan region,the paper discussed how to accelerate the development of rose cultivation and deep processing industry in Hotan region. 展开更多
关键词 Rosa rugosa Thunb. Medicinal and edible plant CULTIVATION Biological characteristics
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