Terpene extract from the stem of Celastrus orbiculatus inhibits actin cytoskeleton remodelling in gastric cancer cells by regulating the protein interaction between PTBP1 and ACTN4

Adjuvant chemoradiotherapy,molecular targeted therapy,and immunotherapy are frequently employed to extend the survival of patients with advanced gastric cancer(GC).However,most of these treatments have toxic side effects,drug resistance,and limited improvements in survival and quality of life.Therefore,it is crucial to discover and develop new medications targeting GC that are highly effective and have minimal toxicity.In previous studies,the total terpene extract from the stem of Celastrus orbiculatus demonstrated anti-GC activity;however,the specific mechanism was unclear.Our research utilising coimmunoprecipitation-mass spectrometry(Co-IP-MS),polypyrimidine tract binding protein 1(ptbp1)clustered regularly interspaced short palindromic repeat-associated protein 9(Cas9)-knockout(KO)mouse model,tissue microarray,and functional experiments suggests that alpha actinin-4(ACTN4)could be a significant biomarker of GC.PTBP1 influences actin cytoskeleton restructuring in GC cells by interacting with ACTN4.Celastrus orbiculatus stem extract(COE)may directly target ACTN4 and affect the interaction between PTBP1 and ACTN4,thereby exerting anti-GC effects.

The role of polyurethane foam compressible layer in the mechanical behaviour of multi-layer yielding supports for deep soft rock tunnels

The polyurethane foam(PU)compressible layer is a viable solution to the problem of damage to the secondary lining in squeezing tunnels.Nevertheless,the mechanical behaviour of the multi-layer yielding supports has not been thoroughly investigated.To fill this gap,large-scale model tests were conducted in this study.The synergistic load-bearing mechanics were analyzed using the convergenceconfinement method.Two types of multi-layer yielding supports with different thicknesses(2.5 cm,3.75 cm and 5 cm)of PU compressible layers were investigated respectively.Digital image correlation(DIC)analysis and acoustic emission(AE)techniques were used for detecting the deformation fields and damage evolution of the multi-layer yielding supports in real-time.Results indicated that the loaddisplacement relationship of the multi-layer yielding supports could be divided into the crack initiation,crack propagation,strain-hardening,and failure stages.Compared with those of the stiff support,the toughness,deformability and ultimate load of the yielding supports were increased by an average of 225%,61%and 32%,respectively.Additionally,the PU compressible layer is positioned between two primary linings to allow the yielding support to have greater mechanical properties.The analysis of the synergistic bearing effect suggested that the thickness of PU compressible layer and its location significantly affect the mechanical properties of the yielding supports.The use of yielding supports with a compressible layer positioned between the primary and secondary linings is recommended to mitigate the effects of high geo-stress in squeezing tunnels.

The impact of N-terminal modification of PAA with different chain lengths on the structure,thermal stability and pH sensitivity of succinylated collagen

The limitations of native collagen,such as thermal stability and solubility in physiological environments,can be improved by applying bioconjugation and synthetic chemistry techniques.However,the exquisite control of the modification site of collagen remains a challenge.In this work,pH-responsive poly(acrylic acid)(PAA)with dif-ferent chain lengths was attached to the N-terminal α-amino groups of succinylated collagen using a site-specific modification strategy.Additionally,the structure,thermal stability,and pH sensitivity of succinylated collagen were explored.The modification rate of amino groups in the succinylated collagen-PAA bioconjugate(SPSC-PAA)was eval-uated by the 2,4,6-trinitrobenzene sulfonic acid assay.The impact of N-terminal modification of PAA and its chain length on the thermal stability of collagen was explored by CD and DSC.These techniques revealed that the ther-mal stability of SPSC-Col is pH-responsive and closely related to the chain length of grafted PAA.The pH sensitivity of SPSC-PAA was further explored by rheology and turbidity.Subsquently,the critical pH and isoelectric point of SPSC-PAAs were also examined by turbidity and isoelectric point titration,respectively.This work provides a new insight into the N-terminal modification of collagen on its properties.

Research on Sleeve Grouting Density Detection Based on the Impact Echo Method

Grouting defects are an inherent challenge in construction practices,exerting a considerable impact on the operational structural integrity of connections.This investigation employed the impact-echo technique for the detection of grouting anomalies within connections,enhancing its precision through the integration of wavelet packet energy principles for damage identification purposes.A series of grouting completeness assessments were meticulously conducted,taking into account variables such as the divergent material properties of the sleeves and the configuration of adjacent reinforcement.The findings revealed that:(i)the energy distribution for the highstrength concrete cohort predominantly occupied the frequency bands 42,44,45,and 47,whereas for other groups,it was concentrated within the 37 to 40 frequency band;(ii)the delineation of empty sleeves was effectively discernible by examining the wavelet packet energy ratios across the spectrum of frequencies,albeit distinguishing between sleeves with 50%and full grouting density proved challenging;and(iii)the wavelet packet energy analysis yielded variable detection outcomes contingent on the material attributes of the sleeves,demonstrating heightened sensitivity when applied to ultrahigh-performance concrete matrices and GFRP-reinforced steel bars.

Sixty years of ethical evolution: The 2024 revision of the Declaration of Helsinki (DoH)

On October 19,2024,the 75th General Assembly of the World Medical Association(WMA)in Finland adopted the latest version of the Declaration of Helsinki(DoH)—Ethical Principles for Medical Research Involving Human Participants[1](hereafter referred to as“the Declaration”).This revision process took 30 months,with the working group comprising representatives from medical associations in 19 countries and regions.From April 2022 to September 2024,the working group held eight regional expert meetings and two times global consultations,gathering suggestions from both experts and the general public[2].Besides,the working group developed regular online meeting working mechanisms.

Empowering health care innovations:A look forward into 2024

As we unveil the first issue of Health Care Science in 2024,it's a moment of reflection and anticipation.Under the astute guidance of our leadership and the collective wisdom of our distinguished Editorial Board Members,we've journeyed through an extraordinary year,marking significant milestones and embracing challenges with resilience and innovation.We believe our progress reflects not just a testament to our commitment but also an indication of our role in driving healthcare innovation and excellence.

How Do College Students Plan Their Future Career Direction under the Global Economic Turmoil

Under the situation of global economic turmoil,the future career planning has become an important issue for college students to think about urgently.At present,the global economy is affected by multiple factors,and traditional industries are facing recession,while emerging industries are booming.In this context,how to choose a university major and plan the future career path has become a key issue.By analyzing the impact of economic turmoil on the career market,this paper discusses how future college students should pay attention to emerging industries and career trends when choosing university majors.At the same time,combined with factors such as digital transformation and globalization trend,the future career direction and skill requirements are clarified,so as to provide college students with planning ideas to deal with the complex economic situation.

3D Seismic Acquisition Technology and Effect in HX Volcanic Area in Liaohe Depression

Multi-stages volcanic are available in HX area, shielding the seismic waves. Previous seismic acquisitions of large size bin, less fold coverage and narrow azimuth result in indistinct fault images, low S/N ratio and the difficulty of multi-stages volcanic characterization. In reference to the successful experience of domestic and overseas volcanic exploration, the low frequency excitation and receiving, and survey with wide range, high coverage, wide azimuth should be paid more attention, associated with two-dimensional and three-dimensional wave equation forward modeling and real data processing contrast analysis method. The image of underlying strata and fault are remarkably improved in the new method, according to the processing results of new seismic data. The new method will provide technical reference for the similar volcanic development area in the future seismic acquisition design.

含磷六元醇的制备及改性水性聚氨酯的结构与性能

以三氯氧磷、丙烯酸羟乙酯和二乙醇胺为原料,合成了一种含磷六羟基多元醇(NP6),将NP6作为内交联剂,异氟尔酮二异氰酸酯、聚丙二醇及二羟甲基丙酸等原料,合成了一系列NP6含量不同的聚氨酯乳液(WPU)。采用红外光谱、核磁共振对NP6和WPU的结构进行了表征,测试了乳液粒径、乳液稳定性和胶膜力学性能,采用热失重分析对乳胶膜的耐热性能进行了分析。结果表明,合成了与设计结构相符的NP6,制备的WPU具有良好的储存稳定性;乳液粒径随NP6含量增加先减小后增大,NP6的加入使乳胶膜的初始模量显著增加;加入NP6后乳胶膜的起始分解温度降低,分解后的残渣量增加。

聚氨酯离子凝胶传感器的制备及性能

以聚氨酯为聚合物网络、离子液体(1-乙基-3-甲基咪唑二氰胺,EMIM:DCA)为导电介质,通过共混后干燥成膜的方式制备了具有不同EMIM:DCA含量的导电离子凝胶。采用万能试验机、电化学工作站和差示扫描量热仪对离子凝胶进行了表征。结果表明,随着EMIM:DCA含量的增加,离子凝胶的力学性能下降,离子电导率上升。其中EMIM:DCA质量分数为30%的离子凝胶(WPU/IL-30)的综合性能最佳,拉伸强度为0.85 MPa,断裂伸长率为264%,离子电导率为0.5 mS/cm,并且具有出色的抗冻性能(Tg=-27.6℃)。利用便携式传感测试仪研究了离子凝胶的应变传感性能,结果表明,WPU/IL-30传感器能够规律且稳定地将人体的运动状态转换为电信号传输到手机终端,且各项性能不会受到储存时间和环境温度的影响。此外,传感器在经过200次50%的循环拉伸后,传感性能依旧保持稳定,具有良好的耐疲劳性。

The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding

Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs.

Effect of terrain on landscape patterns and ecological effects by a gradient-based RS and GIS analysis

Terrain is an important environment factor that plays a vital role in human development by influencing the spatial layout of land use patterns. The terrain niche index, combined with slope and elevation, can comprehensively present detailed information about spatial differences in terrain and is superior to single terrain factors. We applied remote sensing and geographical information system to terrain gradient, used the non-dimensional distribution index to examine spatial distribution characteristics of various landscape types, and analyzed the ecological effects of landscape were quantitatively on terrain gradients. Eco-environment quality (EEQ) was evaluated using the forestry operation area as the evaluation unit. The spatial distributions in various landscapes were significantly influenced by terrain factors, especially by bodies of water, cropland and residential land. The spatial distribution varied greatly in different terrain gradients for similar landscape types. The areas associated with good, intermediate and poor EEQ were occupied primarily by natural landscape, semi-natural and largely artificial landscapes, respectively.

Protein phosphatase PP4 is overexpressed in human breast and lung tumors

Dear Editor, Reversible protein phosphorylation by kinases and phosphatases is one of the most common mechanisms in controlling most, if not all, cellular processes [ 1 ]. Dephos- phorylation of serine/threonine residues is regulated by two distinct groups of functionally diverse serine/threonine protein phosphatases, the PPM family （which includes PP2C） and the PPP family, which includes the type 1 （PP1） and the type 2A （PP2A, PP2B, PP3, PP4, PP5, PP6, and PP7） protein phosphatases. Members of the type 2A group ofphosphatases （PP2A, PP4, and PP6） are the most closely related in sequence. They are sensitive to inhibition by low doses of okadaic acid （OA） or fostriecin, which distinguishes them from PP 1 [ 1 ]. The regulation and function of PP2A have been thoroughly investigated. PP2A is composed of a core complex containing the catalytic subunit （C） and the scaffold subunit （A）, which interacts with a wide variety of regulatory subunits and interacting proteins that target the AC core dimer to specific substrates and subcellular locations [ 1 ]. The assembly of many different types of heterotrimeric holoenzymes accounts for the ability of PP2A, and, possibly, PP4 and PP6, to regulate a wide range of biological orocesses.

PerfMon: Measuring Application-Level Performance in a Large-Scale Campus Wireless Network

WiFi has become one of the most popular ways to access the Internet.However,in large-scale campus wireless networks,it is challenging for network administrators to provide optimized access quality without knowledge on fine-grained traffic characteristics and real network performance.In this paper,we implement PerfMon,a network performance measurement and diagnosis system,which integrates collected multi-source datasets and analysis methods.Based on PerfMon,we first conduct a comprehensive measurement on application-level traffic patterns and behaviors from multiple dimensions in the wireless network of T university(TWLAN),which is one of the largest campus wireless networks.Then we systematically study the application-level network performance.We observe that the application-level traffic behaviors and performance vary greatly across different locations and device types.The performance is far from satisfactory in some cases.To diagnose these problems,we distinguish locations and device types,and further locate the most crucial factors that affect the performance.The results of case studies show that the influential factors can effectively characterize performance changes and explain for performance degradation.

Prediction of Eight Earings in Deep Drawing of 5754O Aluminum Alloy Sheet

Earings appear easily during deep drawing of cylindrical parts owing to the anisotropic properties of materials.However,current methods cannot fully utilize the mechanical properties of material,and the number of earings obtained differ with the simulation methods.In order to predict the eight-earing problem in the cylindrical deep drawing of 5754O aluminum alloy sheet,a new method of combining the yield stress and anisotropy index(r-value)to solve the parameters of the Hil 148 yield function is proposed.The general formula for the yield stress and r-value in any direction is presented.Taking a 5754O aluminum alloy sheet as an example in this study,the deformation area in deep drawing is divided into several equal sectorial regions based on the anisotropy.The parameters of the Hill48 yield function are solved based on the yield stress and r-value simultaneously for the corresponding deformation area.Finite element simulations of deep drawing based on new and existing methods are carried out for comparison with experimental results.This study provides a convenient and reliable way to predict the formation of eight earings in the deep drawing process,which is expected to be useful in industrial applications.The results of this study lay the foundation for the optimization of the cylindrical deep drawing process,including the optimization of the blank shape to eliminate earing defects on the final product,which is of great importance in the actual production process.

Effect of Metaxenia on Sugars and Organic Acids and Taste Quality of Bagged Fuji Apple Fruits

In order to provide reference for the selection of appropriate pollination varieties facilitating the improvement of the flavor quality of bagged Fuji apple, the metaxenia effects of 5 pollination varieties on sugars, organic acids and flavor quality of bagged Fuji fruit were studied. The results showed that the 5 pollination varieties had significant metaxenia effects on sugars, organic acids and flavor quality of bagged Fuji fruit. For the sugar components, the fruits pollinated with Golden Delicious and Jinxiuhaitang showed higher glucose contents, and the fruit pollinated with Gala had the highest sucrose content; the fruits pollinated by Gala, Golden Delicious and Ruby exhibited higher malic acids, and the oxalic acid content and the succinic acid content were higher in the fruits pollinated with Jinxiuhaitang and Ruby ; and the fruit pollinated with Gala had the highest total sugar content, and the total acid content was higher in the fruits pollinated by Jinxiuhaitang and Ruby. The sugar-acid ratio was higher in the fruits pollinated with Gala and Golden Delicious, and the lowest in the fruit pollinated with Ruby. The senso- ry evaluation of fruits showed that the fruit pollinated with Ruby tasted sour-sweet, while the fruits pollinated with other 4 pollination varieties were sour and sweet moderately.

Theoretical analysis on quasi-static lateral compression of elliptical tube between two rigid plates

Based on the rigid plastic theory; the load-deflection functions with and without considering the effect of strain hardening are respectively derived for an elliptical tube under quasi-static compression by two parallel rigid plates. The non-dimensional load-deflection responses predicted by the present theory and the finite element simula- tions are compared, and the favorable agreement is found. The results show that strain hardening may have a noticeable influence on the load-deflection curves of an elliptical tube under quasi-static compression. Compared with the circular counterpart, the ellip- tical tube exhibits different energy absorption behavior due to the difference between the major axis and the minor axis. When loaded along the major axis of a slightly oval tube, a relative even and long plateau region of the load-deflection curve is achieved, which is especially desirable for the design of energy absorbers.

Theoretical model for elliptical tube laterally impacted by two parallel rigid plates

An analytical model is developed to study the crushing behavior and energy absorption capability of a single elliptical tube impacted by two parallel rigid plates, with and without consideration of the strain hardening effect. The four-hinge collapse mechanism is used, and the governing equation is derived from Lagrange equations of the second kind. The numerical simulation of the dynamic response of the elliptical tube under impact using the finite element explicit code LS-DYNA is performed. The reaction force-displacement curve and displacement-time curve of the plate obtained from the two methods are in good agreement.

Effect of Low Temperature Treatment on Agrobacterium-mediated Transformation of Maize Seed Buds

[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud.

Characterization of Regional Combustion Efficiency usingΔXCO:ΔXCO_(2)Observed by a Portable Fourier-Transform Spectrometer at an Urban Site in Beijing

Measurements of column-averaged dry-air mole fractions of carbon dioxide and carbon monoxide,CO_(2)(XCO_(2))and CO(XCO),were performed throughout 2019 at an urban site in Beijing using a compact Fourier Transform Spectrometer(FTS)EM27/SUN.This data set is used to assess the characteristics of combustion-related CO_(2)emissions of urban Beijing by analyzing the correlated daily anomalies of XCO and XCO_(2)(e.g.,ΔXCO andΔXCO_(2)).The EM27/SUN measurements were calibrated to a 125HR-FTS at the Xianghe station by an extra EM27/SUN instrument transferred between two sites.The ratio ofΔXCO overΔXCO_(2)(ΔXCO:ΔXCO_(2))is used to estimate the combustion efficiency in the Beijing region.A high correlation coefficient(0.86)betweenΔXCO andΔXCO_(2)is observed.The CO:CO_(2)emission ratio estimated from inventories is higher than the observedΔXCO:ΔXCO_(2)(10.46±0.11 ppb ppm^(−1))by 42.54%-101.15%,indicating an underestimation in combustion efficiency in the inventories.DailyΔXCO:ΔXCO_(2)are influenced by transportation governed by weather conditions,except for days in summer when the correlation is low due to the terrestrial biotic activity.By convolving the column footprint[ppm(μmol m-2 s-1)-1]generated by the Weather Research and Forecasting-X-Stochastic Time-Inverted Lagrangian Transport models(WRF-X-STILT)with two fossil-fuel emission inventories(the Multi-resolution Emission Inventory for China(MEIC)and the Peking University(PKU)inventory),the observed enhancements of CO_(2)and CO were used to evaluate the regional emissions.The CO_(2)emissions appear to be underestimated by 11%and 49%for the MEIC and PKU inventories,respectively,while CO emissions were overestimated by MEIC(30%)and PKU(35%)in the Beijing area.