Evaluation and analysis of neurocognitive dysfunction in patients with colorectal cancer after radical resection: A retrospective study

BACKGROUND With the continuous progress of colorectal cancer treatment technology,the survival rate of patients has improved significantly,but the problem of postoperative neurocognitive dysfunction has gradually attracted attention.AIM To analyze the risk factors for delayed postoperative neurocognitive recovery(DNR)after laparoscopic colorectal cancer surgery and constructed a risk prediction model to provide an evidence-based reference for the prevention and treatment of DNR after laparoscopic colorectal cancer surgery.METHODS The clinical data of 227 patients with colorectal cancer who underwent laparoscopic surgery and regional cerebral saturation oxygenation(rScO2)monitoring at our hospital from March 2020 to July 2022 were retrospectively analyzed.Common factors and potential factors affecting postoperative DNR were used as analysis variables,and univariate analysis and multifactor analysis were carried out step by step to determine the predictors of the model and construct a risk prediction model.The predictive performance of the model was assessed by the receiver operating characteristic(ROC)curve,the calibration curve was used to assess the fit of the model to the data,and a nomogram was drawn.In addition,30 patients who met the inclusion and exclusion criteria from January 2023 to July 2023 were selected for external verification of the prediction model.RESULTS The incidence of postoperative DNR in the modeling group was 15.4%(35/227).Multivariate analysis revealed that age,years of education,diabetes status,and the lowest rScO2 value were the independent influencing factors of postoperative DNR(all P<0.05).Accordingly,a DNR risk prediction model was constructed after laparoscopic colorectal cancer surgery.The area under the ROC curve of the model was 0.757(95%CI:0.676-0.839,P<0.001),and the Hosmer-Lemeshow test of the calibration curve suggested that the model was well fitted(P=0.516).The C-index for external validation of the row was 0.617.CONCLUSION The DNR risk prediction model associated with rScO2 monitoring can be used for individualized assessment of patients undergoing laparoscopic colorectal cancer surgery and provides a clinical basis for the prevention of DNR after surgery.

大展弦比巡飞弹挂固定翼载机投放研究

大展弦比巡飞弹由于其长航时的优势,在近年的局部冲突中越来越多地得以使用。巡飞弹由固定翼载机挂载投放是其重要的作战方式,由于速度差别,在投放过程中可能面临分离安全问题。本文分析了大展弦比巡飞弹挂固定翼载机投放时减速过程的特性,对基于减速伞的常规减速方式、基于发射筒/减速伞组合的减速方式及弹翼分阶段展开的变构型滑翔减速方式进行仿真分析,为机载巡飞弹投放方式设计提供了思路。

三角形截面长杆弹垂直侵彻半无限靶的成坑分析

为研究三角形截面弹体在垂直侵彻半无限靶时的成坑特性,通过分析三角形截面弹体头部接触面上的横向力,得到弹体头部碎渣颗粒的运动状态。根据侵彻速度关系式推断弹体瞬时速度对弹坑截面形状及大小的影响规律。采用ANSYS-DYNA有限元软件对不同速度的三角形截面弹体垂直侵彻半无限靶全过程进行数值模拟,并进行实验验证。仿真结果与实验结果一致。结果显示,三角形截面弹体在撞击平面上的质点横向应力分布不均匀,3个角方向上的力最大,边长中点位置处最小,总体呈U形分布。弹体瞬时速度逐渐减小,弹坑形状由近似规则圆形逐渐变为近似三角形的3瓣圆弧,且其截面积不断减小。在1 400~1 800 m/s的速度范围内进行了长杆弹撞击实验。速度为1 790 m/s时,弹坑在靶板表面的直径和深度分别为40 mm和7 mm,弹坑形状近似圆形。速度为1 510 m/s时,弹坑在靶板表面的直径和深度分别为38 mm和5 mm,弹坑截面表现为圆弧三角形。

The new X-ray imaging and biomedical application beamline BL13HB at SSRF

A new X-ray imaging and biomedical application beamline(BL13HB)has been implemented at the Shanghai Radiation Synchrotron Facility(SSRF)as an upgrade to the old X-ray imaging and biomedical application beamline(BL13W1).This is part of the Phase II construction project of the SSRF.The BL13HB is dedicated to 2D and 3D static and dynamic X-ray imaging,with a field of view of up to 48.5 mm×5.2 mm and spatial resolution as high as 0.8μm.A super-bending magnet is used as the X-ray source in BL13HB,which has a maximum magnetic field of 2.293 T.The energy range of monochromatic X-ray photons from a double-multiplayer monochromator was 8–40 keV,and the white beam mode was provided on the beamline for dynamic X-ray imaging and dynamic X-ray micro-CT.While maintaining the previous experimental setup of BL13W1,new equipment was added to the beamline experimental station.The beamline is equipped with different sets of X-ray imaging detectors for several experimental methods such as micro-CT,dynamic micro-CT,and pair distribution function.The experimental station of BL13HB is designed specifically for various in situ dynamic experiments,and BL13HB has been open to users since June 2021.

不同补光灯对冬季设施辣椒生长发育及产量的影响

为了解决冬季设施辣椒生产中的光照不足问题,通过引进2种不同补光灯,研究补光对冬季设施辣椒生长发育及产量的影响。结果表明:补光能够促进辣椒生育期的提前,在株高、茎粗、叶片数产量及效益方面均表现为C2(杭州佳遇补光灯)>C1(西安同凯补光灯)>CK(对照),说明补光灯的应用促进了对冬季设施辣椒的生长发育及产量的提高,且C2最优。

近30年思想政治教育载体研究演变探析——基于中国知网和人大复印报刊资料《思想政治教育》的分析

中国共产党一贯重视思想政治教育,将其视作推动中国特色社会主义建设的重要力量。思想政治教育载体是思想政治教育体系中不可或缺的重要因素,是教育者向教育对象传递教育信息、实现教育目标的关键。随着信息技术的发展和应用,思想政治教育载体也在随之发生变化。文章以1992—2018年中国知网CSSCI来源期刊和人大复印报刊资料《思想政治教育》中所刊载的研究思想政治教育载体的文章为研究对象,按照活动载体、文化载体、管理载体和大众传播载体的分类方式,采用计量统计方法,分析各类载体的文章数量和研究频率的变化规律,在此基础上探析思想政治教育载体研究的演变特征、发展趋势及趋势成因。

基于SolidWorks的全地形多用途推车式种树挖坑机结构设计

目前,种树挖坑机存在工作噪音大、使用率低、稳定性差、劳动强度高等问题,大型种树挖坑设备还受到地形及种植要求的限制,不利于推广使用。为解决上述技术问题,利用SolidWorks三维建模并完成全地形多用途推车式种树挖坑机的总体结构设计,该机构具有机动灵活、适应性强的特点,对我国植树造林和果木种植业的发展有一定的实际应用价值。

Genome-wide Analysis of MYB Gene Family in Potato Provides Insights into Tissue-specific Regulation of Anthocyanin Biosynthesis

The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In this study,A total of 217 MYB genes,including 901R-MYBs,124 R2R3-MYBs,and 3 R1R2R3-MYBs have been identified from the potato genome.The 1R-MYB and R2R3-MYB family members could be divided into 20 and 35 subgroups respectively.Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization,further supporting the results of phylogenetic analysis.Potato is an ideal plant to reveal the tissue-specific anthocyanins biosynthesis regulated by MYB,as the anthocyanins could be accumulated in different tissues,showing colorful phenotypes.Five pairs of colored and colorless tissues,stigma,petal,stem,leaf,and tuber flesh,were applied to the transcriptomic analysis.A total of 70 MYB genes were found to be differentially expressed between colored and colorless tissues,and these differentially expressed genes were suspected to regulate the biosynthesis of anthocyanin of different tissues.Co-expression analysis identified numerous potential interactive regulators of anthocyanins biosynthesis,involving 39 MYBs,24 bHLHs,2 WD-repeats,and 29 biosynthesis genes.Genome-wide association study(GWAS)of tuber flesh color revealed amajor signal at the end of Chromosome 10,which was co-localized with reported I gene(StMYB88),controlling tuber peel color.Analyses of DEGs(Differentially Expression Genes)revealed that both StMYB88 and StMYB89 were closely related to regulating anthocyanin biosynthesis of tuber flesh.This work offers a comprehensive overview of the MYB family in potato and will lay a foundation for the functional validation of these genes in the tissue-specific regulation of anthocyanin biosynthesis.

Methodology development and application of X-ray imaging beamline at SSRF

This paper introduces some latest developments regarding the X-ray imaging methodology and applications of the X-ray imaging and biomedical application beamline(BL13W1)at Shanghai Synchrotron Radiation Facility in the past 5 years.The photon energy range of the beamline is 8–72.5 keV.Several sets of X-ray imaging detectors with different pixel sizes(0.19–24 lm)are used to realize X-ray microcomputed tomography(X-ray micro-CT)and X-ray in-line phase-contrast imaging.To satisfy the requirements of user experiments,new X-ray imaging methods and image processing techniques are developed.In vivo dynamic micro-CT experiments with living insects are performed in 0.5 s(sampling rate of 2 Hz,2 tomograms/s)with a monochromatic beam from a wiggler source and in 40 ms(sampling rate of 25 Hz,25 tomograms/s)with a white beam from a bending magnet source.A new X-ray imaging method known as move contrast X-ray imaging is proposed,with which blood flow and moving tissues in raw images can be distinguished according to their moving frequencies in the time domain.Furthermore,X-ray speckle-tracking imaging with twice exposures to eliminate the edge enhancement effect is developed.A high-precision quantification method is realized to measure complex three-dimensional blood vessels obtained via X-ray micro-CT.X-ray imaging methods such as three-dimensional X-ray diffraction microscopy,small-angle X-ray scattering CT,and X-ray fluorescence CT are developed,in which the X-ray micro-CT imaging method is combined with other contrast mechanisms such as diffraction,scattering,and fluorescence contrasts respectively.Moreover,an X-ray nano-CT experiment is performed with a 100 nm spatial resolution.Typical user experimental results from the fields of material science,biomedicine,paleontology,physics,chemistry,and environmental science obtained on the beamline are provided.

Expression of IL-26 predicts prognosis of patients with hepatocellular carcinoma after surgical resection

Background: There is no data regarding prognostic impact of interleukin(IL)-26 on outcomes of patients with hepatocellular carcinoma(HCC). The present study aimed to evaluate the prognostic impact of IL-26 on HCC patients undergoing liver resection. Methods: From 2003 to 2008, 122 patients with HCC who received surgical curative resection were enrolled. Patients were stratified into IL-26-upper and-lower groups according to the median expression level from immunohistochemical staining of resected specimens. Prognostic impact of IL-26 was estimated using Kaplan–Meier curves. Univariate and multivariate analyses were performed to evaluate timedependent prognostic impact and independency of IL-26. Demographic and clinical factors that were associated with IL-26 were comprehensively identified. Results: Prognosis of the patients with high level of IL-26 revealed to be significantly unfavorable in both cumulative recurrence-free survival( P < 0.001) and overall survival( P = 0.002). Upper expression of IL-26(HR: 1.643;95% CI: 1.021 to 2.644;P = 0.041) and microvascular invasion(HR: 3.303;95% CI: 1.255 to 8.696;P = 0.016) were identified as significant independent prognostic factors for overall survival in the multivariable analysis. Conclusions: IL-26 is a novel prognostic factor for HCC after resection. Evaluation of IL-26 expression may be potentially valuable in clinical therapy when planning individualized follow-up schedule and evaluating candidates for prophylactic adjuvant treatment to prevent recurrence.

疏水性银纳米线/纤维素复合纸的制备及电磁屏蔽性能

利用简便、高效的浸渍工艺使银纳米线(AgNW)附着于纤维素纸表面,通过表面疏水改性,成功制备出疏水性AgNW/纤维素复合纸。研究发现,AgNW在纤维素纸表面形成了致密的导电网络,在低填料含量下赋予纤维素纸超高的导电性能。当浸渍10次(AgNW体积分数仅为1.18%)时,复合纸面内方向导电率可达33.69 S/cm。由于2个高导电性外表面对电磁波的多次反射和散射,所得到的复合材料对X波段(8.2~12.4 GHz)的总电磁屏蔽效能(EMI SE)高达40 dB(材料厚度仅为0.2 mm)。此外,外部疏水涂层使复合纸的表面水接触角高达140°,显示出良好的防水抗污和对AgNW的耐氧防护功能。该复合纸可望在高湿度环境下应用于具有自清洁功能的电子器件屏蔽包装。

Regioisomeric Polymer Semiconductors Based on Cyano-Functionalized Dialkoxybithiophenes:Structure-Property Relationship and Photovoltaic Performance

Cyano substitution is vital to the molecular design of polymer semiconductors toward highly efficient organic solar cells.However,how regioselectivity impacts relevant optoelectronic properties in cyano-substituted bithiophene systems remain poorly understood.Three regioisomeric cyano-functionalized dialkoxybithiophenes BT_(HH),BT_(HT),and BT_(TT) with headto-head,head-to-tail,and tail-to-tail linkage,respectively,were synthesized and characterized in this work.The resulting polymer semiconductors(PBDTBTs)based on these building blocks were prepared accordingly.The regiochemistry and property relationships of PBDTBTs were investigated in detail.The BTHH moiety has a higher torsional barrier than the analogs BT_(HT) and BT_(TT),and the regiochemistry of dialkoxybithiophenes leads to fine modulation in the optoelectronic properties of these polymers,such as optical absorption,band gap,and energy levels of frontier molecular orbitals.Organic field-effect transistors based on PBDTBT_(HH) had higher hole mobility(4.4×10^(-3) cm^(2)/(V·s))than those(ca.10^(-4) cm^(2)/(V·s))of the other two polymer analogs.Significantly different short-circuit current densities and fill factors were obtained in polymer solar cells using PBDTBTs as the electron donors.Such difference was probed in greater detail by performing space-charge-limited current mobility,thin-film morphology,and transient photocurrent/photovoltage characterizations.The findings highlight that the BTHH unit is a promising building block for the construction of polymer donors for highperformance organic photovoltaic cells.

Hexagonal arrangement of phospholipids in bilayer membranes

The phospholipid membrane plays a key role in myriad biological processes and phenomena, and the arrangement structure of membrane determines its function. However, the molecular arrangement structure of phospholipids in cell membranes is difficult to detect experimentally. On the basis of molecular dynamic simulations both in a non-destructive way and at native environment, we observed and confirmed that the phospholipids self-assemble to a hexagonal arrangement structure under physiological conditions. The underlying mechanism was revealed to be that there are hexagonal arrangement regions with a lower free energy around each lipid molecule. The findings potentially advance the understanding of biological functions of phospholipid bilayers.

Evolutionary impacts of purine metabolism genes on mammalian oxidative stress adaptation

Many mammals risk damage from oxidative stress stemming from frequent dives(i.e., cycles of ischemia/reperfusion and hypoxia/reoxygenation),high altitude and subterranean environments, or powered flight. Purine metabolism is an essential response to oxidative stress, and an imbalance between purine salvage and de novo biosynthesis pathways can generate damaging reactive oxygen species(ROS). Here, we examined the evolution of 117 purine metabolism-related genes to explore the accompanying molecular mechanisms of enhanced purine metabolism in mammals under high oxidative stress. We found that positively selected genes,convergent changes, and nonparallel amino acid substitutions are possibly associated with adaptation to oxidative stress in mammals. In particular, the evolution of convergent genes with c AMP and c GMP regulation roles may protect mammals from oxidative damage. Additionally, 32 genes were identified as under positive selection in cetaceans, including key purine salvage enzymes(i.e., HPRT1), suggesting improved re-utilization of non-recyclable purines avoid hypoxanthine accumulation and reduce oxidative stress. Most intriguingly, we found that six unique substitutions in cetacean xanthine dehydrogenase(XDH), an enzyme that regulates the generation of the ROS precursor xanthine oxidase(XO) during ischemic/hypoxic conditions, show enhanced enzyme activity and thermal stability and diminished XO conversion activity. These functional adaptations are likely beneficial for cetaceans by reducing radical oxygen species production during diving. In summary, our findings offer insights into the molecular and functional evolution of purine metabolism genes in mammalian oxidative stress adaptations.

种树机减速器用齿轮坯热锻模具磨损的有限元仿真分析

以某型种树机减速器用齿轮坯为研究对象,利用DEFORM-3D有限元数值模拟软件对其精密塑性成形过程进行仿真,分析了预热温度摩擦因子、压机工作速度对模具最大磨损量的影响。在预热温度300℃,摩擦因子0.3,压机工作速度50 mm/s的情况下进行了模具生产试制。试制发现,模具磨损量较小,锻件成形质量良好,模具寿命较长。

安徽省重点监控用水单位监督管理工作研究

本文归纳分析了安徽省重点监控用水单位监督管理工作中名录管理制度、取用水计量监控措施、用水效率监控、节水用水激励约束机制等方面现状及存在的问题,提出了相关建议,为系统推进和规范重点监控用水单位的监督管理工作提供参考。

原子钼催化剂配位结构的动态演变促进氢分子在浆态床加氢裂化过程中的高效活化

开发具有高原子利用率和高转化频率的高效催化剂,用于减压渣油浆态床加氢裂化过程中H2分子的活化至关重要.本文采用“聚合-热解-原位硫化”策略,首次报道了一种高效稳定的单原子钼催化剂用于减压渣油浆态床加氢裂化反应,并发现了单原子Mo活性位点的原子配位结构动态演化机制.在VR加氢裂化过程中,与Mo配位的O原子逐渐被S原子取代,从而导致O/S交换过程.单原子钼的配位结构从反应前的Mo-O3S1演变为反应后的Mo-O1S3配位构型,有效促进了H2分子均裂活化为H自由基物种.所演变的单原子钼催化剂表现出优异的催化加氢活性和循环稳定性,其对减压渣油的单程转化率为65 wt.%,轻油收率为93 wt.%,焦炭产率仅为0.63 wt.%,按照所有金属计算的转化频率为0.35 s^(-1).理论计算表明,H2相互作用前后Mo 4d占据态的显著变化与单原子钼催化剂结构的动态演变有直接关系,并且Mo-O1S3位点较低的d带中心表明原子H扩散容易,有利于催化加氢.本文研究结果对于开发高原子经济性催化剂用于重油改质技术的工业应用具有重要意义.

Artificial cilia for soft and stable surface covalent immobilization of bone morphogenetic protein-2

Preservation of growth factor sensitivity and bioactivity(e.g.,bone morphogenetic protein-2(BMP-2))post-immobilization to tissue engineering scaffolds remains a great challenge.Here,we develop a stable and soft surface modification strategy to address this issue.BMP-2(a model growth factor)is covalently immobilized onto homogeneous poly(glycidyl methacrylate)(PGMA)polymer brushes which are grafted onto substrate surfaces(Au,quartz glass,silica wafer,or common biomaterials)via surface-initiated atom transfer radical polymerization.This surface modification method multiplies the functionalized interfacial area;it is simple,fast,gentle,and has little effect on the loaded protein owing to the cilia motility.The immobilized BMP-2(i-BMP-2)on the surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity(-87%bioactivity of free BMP-2 in vitro and 20%-50%higher than scaffolds with free BMP-2 in vivo),with conformation and secondary structure well-preserved after covalent immobilization and ethanol sterilization.Moreover,the osteogenic activity of i-BMP-2 on the nanoline pattern(PGMA-poly(N-isopropylacrylamide))shows-110%bioactivity of free BMP-2.This is superior compared to conventional protein covalent immobilization strategies in terms of both bioactivity preservation and therapeutic efficacy.PGMA polymer brushes can be used to modify surfaces of different tissue-engineered scaffolds,which facilitates in situ immobilization of growth factors,and accelerates repair of a wide range of tissue types.

Wip1 inhibits neutrophil extracellular traps to promote abscess formation in mice by directly dephosphorylating Coronin-1a

Neutrophil extracellular traps (NETs) participate in the rapid inhibition and clearance of pathogens during infection;however, the molecular regulation of NET formation remains poorly understood. In the current study, we found that inhibition of the wild-type p53-induced phosphatase 1 (Wip1) significantly suppressed the activity of Staphylococcus aureus (S. aureus) and accelerated abscess healing in S. aureus-induced abscess model mice by enhancing NET formation. A Wip1 inhibitor significantly enhanced NET formation in mouse and human neutrophils in vitro. High-resolution mass spectrometry and biochemical assays demonstrated that Coro1a is a substrate of Wip1. Further experiments also revealed that Wip1 preferentially and directly interacts with phosphorylated Coro1a than compared to unphosphorylated inactivated Coro1a. The phosphorylated Ser426 site of Coro1a and the 28–90 aa domain of Wip1 are essential for the direct interaction of Coro1a and Wip1 and for Wip1 dephosphorylation of p-Coro1a Ser426. Wip1 deletion or inhibition in neutrophils significantly upregulated the phosphorylation of Coro1a-Ser426, which activated phospholipase C and subsequently the calcium pathway, the latter of which promoted NET formation after infection or lipopolysaccharide stimulation. This study revealed Coro1a to be a novel substrate of Wip1 and showed that Wip1 is a negative regulator of NET formation during infection. These results support the potential application of Wip1 inhibitors to treat bacterial infections.

CCL5 deficiency promotes liver repair by improving inflammation resolution and liver regeneration through M2 macrophage polarization

Despite the diverse etiologies of drug-induced liver injury(DILI),innate immunity activation is a common feature involved in DILI progression.However,the involvement of innate immunity regulation in inflammation resolution and liver regeneration in DILI remains obscure.Herein,we identified the chemokine CCL5 as a central mediator of innate immunity regulation in the pathogenesis of DILI.First,we showed that serum and hepatic CCL5 levels are elevated in both DILI patients and an APAP-induced liver injury(AILI)mouse model.Interestingly,both nonparenchymal cells and stressed hepatocytes are cell sources of CCL5 induction in response to liver injury.Functional experiments showed that CCL5 deficiency has no effect on the early phase of AILI but promotes liver repair in the late phase mainly by promoting inflammation resolution and liver regeneration,which are associated with an increased number of hepatic M2 macrophages.Mechanistically,CCL5 can directly activate M1 polarization and impede M2 polarization through the CCR1-and CCR5-mediated activation of the MAPK and NF-κB pathways.We then showed that CCL5 inhibition mediated by either a CCL5-neutralizing antibody or the antagonist Met-CCL5 can greatly alleviate liver injury and improve survival in an AILI mouse model.Our data demonstrate CCL5 induction during DILI,identify CCL5 as a novel innate immunity regulator in macrophage polarization,and suggest that CCL5 blockage is a promising therapeutic strategy for the treatment of DILI.