A Comparison of Co-temporal Vector Magnetograms Obtained with HMI/SDO and SP/Hinode

Accurate measurement of magnetic fields is very important for understanding the formation and evolution of solar magnetic fields.Currently,there are two types of solar magnetic field measurement instruments:filter-based magnetographs and Stokes polarimeters.The former gives high temporal resolution magnetograms and the latter provides more accurate measurements of magnetic fields.Calibrating the magnetograms obtained by filter-based magnetographs with those obtained by Stokes polarimeters is a good way to combine the advantages of the two types.Our previous studies have shown that,compared to the magnetograms obtained by the Spectro-Polarimeter(SP)on board Hinode,those magnetograms obtained by both the filter-based Solar Magnetic Field Telescope(SMFT)of the Huairou Solar Observing Station and by the filter-based Michelson Doppler Imager(MDI)aboard SOHO have underestimated the flux densities in their magnetograms and systematic center-to-limb variations present in the magnetograms of both instruments.Here,using a sample of 75 vector magnetograms of stable alpha sunspots,we compare the vector magnetograms obtained by the Helioseismic and Magnetic Imager(HMI)aboard Solar Dynamics Observatory(SDO)with co-temporal vector magnetograms acquired by SP/Hinode.Our analysis shows that both the longitudinal and transverse flux densities in the HMI/SDO magnetograms are very close to those in the SP/Hinode magnetograms and the systematic center-to-limb variations in the HMI/SDO magnetograms are very minor.Our study suggests that using a filter-based magnetograph to construct a low spectral resolution Stokes profile,as done by HMI/SDO,can largely remove the disadvantages of the filter-type measurements and yet still possess the advantage of high temporal resolution.

DFDVis:A Visual Analytics System for Understanding the Semantics of Data Flow Diagram

Data flow diagram(DFD),as a special kind of data,is a design artifact in both requirement analysis and structured analysis in software development.However,rigorous analysis of DFD requires a formal semantics.Formal representation of DFD and its formal semantics will help to reduce inconsistencies and confusion.The logical structure of DFD can be described using formalism of Calculus of Communicating System(CCS).With a finite number of states based on CCS,state space methods will help a lot in analysis and verification of the behavior of the systems.But the number of states of even a relatively small system is often very great that is called state explosion.In this paper,we present a visual system which combines Formal methods and visualization techniques so as to help the researchers to understand and analyze the system described by the DFD regardless of the problem of state explosion.

Establishing cell suitability for high-level production of licorice triterpenoids in yeast

Yeast has been an indispensable host for synthesizing complex plant-derived naturalcompounds, yet the yields remained largely constrained. This limitation mainly arises from overlookingthe importance of cell and pathway suitability during the optimization of enzymes and pathways. Herein,beyond conventional enzyme engineering, we dissected metabolic suitability with a framework forsimultaneously augmenting cofactors and carbon flux to enhance the biosynthesis of heterogenoustriterpenoids. We further developed phospholipid microenvironment engineering strategies, dramaticallyimproving yeast’s suitability for the high performance of endoplasmic reticulum (ER)-localized, ratelimitingplant P450s. Combining metabolic and microenvironment suitability by manipulating only threegenes, NHMGR (NADH-dependent HMG-CoA reductase), SIP4 (a DNA-binding transcription factor)andGPP1 (Glycerol-1-phosphate phosphohydrolase 1), we enabled the high-level production of 4.92 g/L rarelicorice triterpenoids derived from consecutive oxidation of b-amyrin by two P450 enzymes afterfermentation optimization. This production holds substantial commercial value, highlighting the criticalrole of establishing cell suitability in enhancing triterpenoid biosynthesis and offering a versatileframework applicable to various plant natural product biosynthetic pathways.

SARS-CoV-2 variants,immune escape,and countermeasures

Coronavirus disease 2019(COVID-19)has become a global pandemic disease.SARS-CoV-2 variants have aroused great concern and are expected to continue spreading.Although many countries have promoted rollout vaccination,the immune barrier has not yet been fully established,indicating that populations remain susceptible to infection.In this review,we summarize the literature on variants of concern and focus on the changes in their transmissibility,pathogenicity,and resistance to the immunity constructed by current vaccines.Furthermore,we analyzed relationships between variants and breakthrough infections,as well as the paradigm of new variants in countries with high vaccination rates.Terminating transmission,continuing to strengthen variant surveillance,and combining nonpharmaceutical intervention measures and vaccines are necessary to control these variants.

Catalytic Benzylation Reactions: From C-H Bond Activation to C-N Bond Activation

Transition-metal mediated activation of inert chemical bonds is an ongoing topic in homogeneous catalysis.In view of the abundance and accessibility of alkylarenes and benzylamines,the use of them as benzyl source in catalytic benzylation reactions via benzylic C-H and C-N bond activation is highly desirable.Indeed,compared with the traditional approaches with benzyl halide as the substrates,benzylation reactions via C-H and C-N bond cleavage provide more efficient,atom-economic strategies to access myriads of synthetically important molecules.In this account,our group's efforts on catalytic benzylation reactions via directed C-H activation,nondirected C-H activation and C-N bond activation are summarized.

Palladium-Catalyzed Aminomethylation of Nitrodienes and Dienones via Double C-N Bond Activation

Main observation and conclusion A new strategy for the generation of the active Pd-alkyl species from aminal via C-N bond activation has been established,in which the formation of zwitterionic intermediate through aza-Michael addition of aminal to nitrodienes or dienones is identified as a key step for the activation of the C-N bond.The efficient strategy has enabled a new palladium-catalyzed a-aminomethylation of nitro-dienes and dienones via double C-N bond activation.The scope and versatility of the reaction were demonstrated and a broad range of substrates bearing electron-donating and-withdrawing groups on the aromatic rings were all compatible with this reaction to furnish the desired a-aminomethylated products in moderate to good yields with excellent regioselectivities and E/Z selectivities.