Spatial Structure of Tourism Resources in Liaoning Province

Selecting fifty one 4A grade scenic spots in Liaoning Province as sample scenic spots,this paper analyzes spatial structure of tourism resources of Liaoning Province in quantitative and qualitative aspects by means of geographical and mathematical spatial analysis method. Results indicate that spatial distribution of resource type scenic spots is agglomerative,and the balance of regional distribution is not high; the degree of centralized distribution is relatively low,but it is still centralized distribution,the distribution uniformity is relatively low. Areas with high density of resource type scenic spot distribution include Shenyang,Dalian,and Dandong cities in Liaoning Province.

Mutations in the miRNA165/166 binding site of the HB2 gene result in pleiotropic effects on morphological traits in wheat

Leaf,spike,stem,and root morphologies are key factors that determine crop growth,development,and productivity.Multiple genes that control these morphological traits have been identified in Arabidopsis,rice,maize,and other plant species.However,little is known about the genomic regions and genes associated with morphological traits in wheat.Here,we identified the ethyl methanesulfonate-derived mutant wheat line M133 that displays multiple morphological changes that include upward-curled leaves,paired spikelets,dwarfism,and delayed heading.Using bulked segregant RNA sequencing(BSR-seq)and a high-resolution genetic map,we identified TraesCS1D02G155200(HBD2)as a potential candidate gene.HB-D2 encodes a class III homeodomain-leucine zipper(HD-ZIP III)transcription factor,and the mutation was located in the miRNA165/166 complementary site,resulting in a resistant allele designated rHb-D2.The relative expression of rHb2 in the mutant plants was significantly higher(P<0.01)than in plants homozygous for the WT allele.Independent resistant mutations that disrupt the miRNA165/166 complementary sites in the A-(rHb-A2)and B-genome(rHb-B2)homoeologs showed similar phenotypic alterations,but the relative intensity of the effects was different.Transgenic plants expressing rHb-D2 gene driven by the maize UBIQUITIN(UBI)promoter showed similar phenotypes to the rHb-D2 mutant.These results confirmed that HB-D2 is the causal gene responsible for the mutant phenotypes.Finally,a survey of 1397 wheat accessions showed that the complementary sites for miRNA165/166 in all three HB2 homoeologs are highly conserved.Our results suggest that HB2 plays an important role in regulating growth and development in wheat.

Distribution of antibiotic resistant bacteria in different soil types following manure application

ARB was investigated in different soil types following manure application.CTC-manure induced more resistance of soil indigenous microbes in fluvo-aquic soil.Lactobacillus,Dyella,Ralstonia,and Bacillus were the key different genera.Manure control is an effective way to reduce the risk of soil ARB.

High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1

Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.

An Analysis on Rural Tourism Development Model in Yi County of Huangshan City

The Yi County's rural tourism model has changed a lot,gradually transferring from the single model to the composite model,which integrates sightseeing,touring,entertainment,leisure,vocation,and shopping. This article mainly focuses on the rural tourism development model. Yi County is a typical case for others,which gives a new development model.

The Spatial Structure of Rural Recreational Tourism Destinations

We select 149 rural recreational tourism destinations in Liaoning Province as the samples, and use geographical mathematical method to analyze the travel spatial structure of rural recreational tourism destinations from aggregation degree of spatial distribution, the balance of spatial distribution, spatial connection and spatial accessibility. The results show that the spatial distribution of tourism destinations shows the gathering distribution pattern, and the evenness of the distribution is very low, displaying strong neighboring positive effect; the accessibility of traffic network is high, and basically the dense tourist traffic network is formed; the nodes with the best accessibility are mainly concentrated in the city groups of central Liaoning and the southern regions of Liaoning, while the nodes with poor accessibility are mainly concentrated in the city groups of western Liaoning and the eastern regions of Liaoning.

Analysis on Development of Rural Financial Management Informationization

With the constant development of China's economy,rural financial management information gradually plays a major role in China's economic development. This paper mainly studied related management information of accounting information system and main characteristics of rural management. On the basis of analysis,it came up with pertinent recommendations,including improving software for financial informationization,improving the quality of rural financial personnel,strengthening government support for rural financial management informationization,and enhancing popularization of accounting computerized software.

Mutations in wheat TaAPA2 gene result in pleiotropic effects on plant architecture

Dear Editor,Bread wheat(Triticum aestivum L.)is a globally important cereal providing~20% of the calories and proteins for>4.5billion people.Plant architecture,including morphologies of leaves,spikes,stems,and roots,has great impact on plant development and productivity,and thus has been extensively investigated in various plant species(Jiang et al.,2023;Zhang et al.,2017).

A near-complete genome sequence of einkorn wheat provides insight into the evolution of wheat A subgenomes

Dear Editor,Triticum monococcum subsp.monococcum(2n=2x=14,AmAm),commonly known as einkorn wheat,is one of the oldest cereal crops and was domesticated in southeastern Turkey about 12000 years ago(Heun et al.,1997).This species played a significant role in the development of agriculture and was widely cultivated for centuries in the Middle East,Europe,Central Asia,and Africa before being replaced by free-threshing polyploid wheats.Triticum monococcum is closely related to T.urartu(genome Au Au),the donor of the A genome in durum and bread wheat(Dvorak et al.,1988).