The wall wettability of microchannels plays an important role in the gas-liquid mass transfer dynamics under Taylor flow.In this study,we regulated the contact angle of the wall surface through surface chemical grafti...The wall wettability of microchannels plays an important role in the gas-liquid mass transfer dynamics under Taylor flow.In this study,we regulated the contact angle of the wall surface through surface chemical grafting polymerization under controlled experimental conditions.The dynamic changes of CO_(2)bubbles flowing along the microchannel were captured by a high-speed video camera mounted on a stereo microscope,whilst a unit cell model was employed to theoretically investigate the gas-liquid mass transfer dynamics.We quantitatively characterized the effects of wall wettability,specifically the contact angle,on the formation mechanism of gas bubbles and mass transfer process experimentally.The results revealed that the gas bubble velocity,the overall volumetric liquid phase mass transfer coefficients(kLa),and the specific interfacial area(a)all increased with the increase of the contact angle.Conversely,gas bubble length and leakage flow decreased.Furthermore,we proposed a new modified model to predict the gas-liquid two-phase mass transfer performance,based on van Baten’s and Yao’s models.Our proposed model was observed to agree reasonably well with experimental observations.展开更多
Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affectin...Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affecting plant growth and development.In this study,the effects of paclobutrazol on the model diatom Phaeodactylum tricornutum were investigated.Results show that 2.5-mg/L and 10-mg/L paclobutrazol significantly inhibited the algal growth by inhibiting chlorophyll synthesis,which affects photosynthesis.The antioxidant system,including catalase(CAT)and glutathione peroxidase(GPx)was severely damaged.Chrysolaminarin content was significantly elevated and doubled up to 127 mg/g dry cell weight(DCW)by 10-mg/L paclobutrazol treatment.In combination with transcriptomic analysis,paclobutrazol was demonstrated to play a regulatory role in the accumulation of chrysolaminarin and neutral lipids.展开更多
The dinoflagellates Alexandrium pacificum and Prorocentrum donghaiense are two well-known harmful algal blooms(HABs)-forming species,both were usually found in the same sea areas in form of HABs in China.To date,there...The dinoflagellates Alexandrium pacificum and Prorocentrum donghaiense are two well-known harmful algal blooms(HABs)-forming species,both were usually found in the same sea areas in form of HABs in China.To date,there is no mechanistic model that can fully explain the occurrence of P.donghaiense blooms with A.pacificum.We found that different strains of P.donghaiense had different intrinsic growth rates of 0.107-0.215/d,and these strains exhibited different responses to the allelopathic action from A.pacificum.Some strains of P.donghaiense could grow well despite some degrees of inhibition in a short period,suggesting the two algal species P.donghaiense and A.pacificum could coexist,even if A.pacificum was allelopathic.Our findings may advance the understanding of phenotypes in P.donghaiense and provide a potential mechanism involved in the coexistence of P.donghaiense and A.pacificum in the same area.展开更多
Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rate...Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rates.Negative pressure wound therapy(NPWT)is one of the most effective techniques for the treatment of diabetic foot wounds and great progress,both in terms of research and its clinical application,has been made in the last 20 years of its development.However,due to the complex pathogenesis and management of diabetic foot,irregular application of NPWT often leads to complications,such as infection,bleeding and necrosis,that seriously affect its treatment outcomes.In 2020,under the leadership of Burns,Trauma and Tissue Repair Committee of the Cross-Straits Medicine Exchange Association,the writing group for‘Consensus on the application of negative pressure wound therapy of diabetic foot wounds’was established with the participation of scholars from the specialized areas of burns,endocrinology,vascular surgery,orthopedics and wound repair.Drawing on evidence-based practice suggested by the latest clinical research,this consensus proposes the best clinical practice guidelines for the application and prognostic evaluation of NPWT for diabetic foot.The consensus aims to support the formation of standardized treatment schemes that clinicians can refer to when treating cases of diabetic foot.展开更多
Science and technology talent evaluation is an important part of the talent development system and mechanism,and the state's emphasis on science and technology talent evaluation is reflect-ed in the national polic...Science and technology talent evaluation is an important part of the talent development system and mechanism,and the state's emphasis on science and technology talent evaluation is reflect-ed in the national policy.This paper takes 49 policy documents on science and technology tal-ent evaluation issued by the state from 1978 to 2022 as the object,and uses policy text analysis,word frequency analysis and co-word network analysis to interpret the content of the policies.These policy texts are divided into four stages.200 high-frequency words were extracted from the policies in each stage after word segmentation was made,and the co-word matrixes and co-word networks were constructed for each stage.The research draws the following conclu-sions:1)The content of Chinese science and technology talent evaluation policies is gradually becoming concrete,and the concept of science and technology talent evaluation is gradually clear;2)The policies have transitioned from"scientific and technological development"to"sci-entific and technological innovation";3)The distribution of policies with different strengths is uneven;4)The cooperation between all departments involved in policy formulation is not e-nough.展开更多
Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of...Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.展开更多
Phyllosticta species associated with diseases of four commercial Citrus species grown in China are reported.Totally,496 Phyllosticta strains were isolated from mandarins(Citrus reticulata),pomeloes(C.maxima),oranges(C...Phyllosticta species associated with diseases of four commercial Citrus species grown in China are reported.Totally,496 Phyllosticta strains were isolated from mandarins(Citrus reticulata),pomeloes(C.maxima),oranges(C.sinensis)and lemons(C.limon)in the main citrus producing regions across China,and 74 strains were selected for phylogenetic analysis.Analyses inferred from the sequences of internal transcribed spacer region(ITS1,5.8S nrDNA and ITS2),partial translation elongation factor 1-alpha(TEF1)and partial actin gene(ACT),showed these representative Phyllosticta isolates clustered in four distinct clades corresponding to three known,and one undescribed species.The newly resolved taxon,Phyllosticta citrichinaensis was isolated from leaves and fruits of all four Citrus species and is introduced in this paper.This taxon caused minor damage,showing irregular spots or freckles.Phyllosticta citriasiana,associated with tan spot of pomeloes,was isolated only from pomeloes,and never from lemons,mandarins and oranges.Phyllosticta citricarpa,the citrus black spot pathogen,which is presently subjected to phytosanitary legislation in the EU and United States,was isolated from lemons,mandarins and oranges,but never from pomeloes.The isolates of P.citricarpa clustered in two subclades,one from mandarins,the other from oranges and lemons.P.capitalensis was isolated from all four Citrus species as an endophyte,causing false melanose,or together with P.citricarpa or P.citriasiana.Morphological,cultural and biochemical characters were consistent with the results of phylogenetic analysis.In addition,a specific primer pair Pca8/ITS4 was designed and selected,and its corresponding PCR procedure was developed for the detection of P.citriasiana in this study.展开更多
We fabricate a pair of fiber Bragg gratings(FBGs)by a visible femtosecond laser phase mask scanning technique on passive large-mode-area double-cladding fibers for multi-kilowatt fiber oscillators.The bandwidth of hig...We fabricate a pair of fiber Bragg gratings(FBGs)by a visible femtosecond laser phase mask scanning technique on passive large-mode-area double-cladding fibers for multi-kilowatt fiber oscillators.The bandwidth of high-reflection(HR)and lowreflection(LR)FBG is~1.6 nm and 0.3 nm,respectively.The reflection of the HR-FBG is higher than 99%,and that of the LR-FBG is about 10%.A bidirectional pumped all-fiber oscillator is constructed using this pair of FBGs,a record output power of 5027 W located in the signal core is achieved with a slope efficiency of~82.1%,and the beam quality factor M2is measured to be~1.6 at the maximum power.The FBGs are simply fixed on a water cooling plate without a special package,and the thermal efficiency of the HR-FBG and the LR-FBG is 2.76℃/kW and 1℃/kW,respectively.Our research provides an effective solution for robust high-power all-fiber laser oscillators.展开更多
Side pumping combiners are widely used in fiber laser schemes for their high coupling efficiency, low insertion loss, and multi-point pumping capability. However, side pumping combiners perform differently in coupling...Side pumping combiners are widely used in fiber laser schemes for their high coupling efficiency, low insertion loss, and multi-point pumping capability. However, side pumping combiners perform differently in coupling efficiency when pumping with a laser diode(LD) and a high-brightness 1018 nm Yb-doped fiber laser(YDFL). In this paper, for the first time, to the best of our knowledge, we investigated the different parameters to fabricate the(2 + 1) × 1 combiner with high coupling efficiency when pumping with an LD and a YDFL, respectively. After optimization, the maximum coupled pump power from one single-pump port of the combiner was 1200 W and 2730 W when pumping with a LD and a YDFL, respectively.展开更多
We propose here a novel method for position fixing in the micron scale by combining the convolutional neural network(CNN) architecture and speckle patterns generated in a multimode fiber. By varying the splice offset ...We propose here a novel method for position fixing in the micron scale by combining the convolutional neural network(CNN) architecture and speckle patterns generated in a multimode fiber. By varying the splice offset between a single mode fiber and a multimode fiber, speckles with different patterns can be generated at the output of the multimode fiber. The CNN is utilized to learn these specklegrams and then predict the offset coordinate. Simulation results show that predicted positions with the precision of 2 μm account for 98.55%.This work provides a potential high-precision two-dimensional positioning method.展开更多
Microalgae harvesting remains a challenging step in microalgae industrialization,thereby provoking the necessity to explore sustainable and economically feasible approaches.This research investigated the use of magnet...Microalgae harvesting remains a challenging step in microalgae industrialization,thereby provoking the necessity to explore sustainable and economically feasible approaches.This research investigated the use of magnetic flocculated nanoparticles in the harvesting of the common microalgae Chlorella pyrenoidosa and Scenedesmus obliquus.The results showed that magnetic flocculated nanoparticles efficiently adsorbed negatively charged microalgae cells,and a magnetic field could adsorb the magnetic flocculated nanoparticles,thereby harvesting the microalgae cells.Harvesting efficiency was remarkably increased at the optimum magnetic field strength of 0.5 T with the magnetic flocculated nanoparticles at 0.738 g/L,and microalgae broth at pH 9.0,whereas the recovery rates of both C.pyrenoidosa and S.obliquus were around 97%and the sedimentation speed of both was above 2.63 cm/min.This study exemplified the magnetic flocculated nanoparticles-based approach to effectively harvest the microalgae cells.展开更多
基金the financial supports from National Natural Science Foundation of China(21978250,22208278)Natural Science Foundation of Shandong Province(ZR2020KB013,ZR2020QE211,2019KJC012).
文摘The wall wettability of microchannels plays an important role in the gas-liquid mass transfer dynamics under Taylor flow.In this study,we regulated the contact angle of the wall surface through surface chemical grafting polymerization under controlled experimental conditions.The dynamic changes of CO_(2)bubbles flowing along the microchannel were captured by a high-speed video camera mounted on a stereo microscope,whilst a unit cell model was employed to theoretically investigate the gas-liquid mass transfer dynamics.We quantitatively characterized the effects of wall wettability,specifically the contact angle,on the formation mechanism of gas bubbles and mass transfer process experimentally.The results revealed that the gas bubble velocity,the overall volumetric liquid phase mass transfer coefficients(kLa),and the specific interfacial area(a)all increased with the increase of the contact angle.Conversely,gas bubble length and leakage flow decreased.Furthermore,we proposed a new modified model to predict the gas-liquid two-phase mass transfer performance,based on van Baten’s and Yao’s models.Our proposed model was observed to agree reasonably well with experimental observations.
基金Supported by the National Natural Science Foundation of China (Nos.31870027,42006125)the Guangdong Natural Science Foundation (No.2019B1515120062)。
文摘Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affecting plant growth and development.In this study,the effects of paclobutrazol on the model diatom Phaeodactylum tricornutum were investigated.Results show that 2.5-mg/L and 10-mg/L paclobutrazol significantly inhibited the algal growth by inhibiting chlorophyll synthesis,which affects photosynthesis.The antioxidant system,including catalase(CAT)and glutathione peroxidase(GPx)was severely damaged.Chrysolaminarin content was significantly elevated and doubled up to 127 mg/g dry cell weight(DCW)by 10-mg/L paclobutrazol treatment.In combination with transcriptomic analysis,paclobutrazol was demonstrated to play a regulatory role in the accumulation of chrysolaminarin and neutral lipids.
基金Supported by the National Key Research and Development Program of China(No.2017YFC1404301)the National Natural Science Foundation of China(Nos.41230961,41176088)。
文摘The dinoflagellates Alexandrium pacificum and Prorocentrum donghaiense are two well-known harmful algal blooms(HABs)-forming species,both were usually found in the same sea areas in form of HABs in China.To date,there is no mechanistic model that can fully explain the occurrence of P.donghaiense blooms with A.pacificum.We found that different strains of P.donghaiense had different intrinsic growth rates of 0.107-0.215/d,and these strains exhibited different responses to the allelopathic action from A.pacificum.Some strains of P.donghaiense could grow well despite some degrees of inhibition in a short period,suggesting the two algal species P.donghaiense and A.pacificum could coexist,even if A.pacificum was allelopathic.Our findings may advance the understanding of phenotypes in P.donghaiense and provide a potential mechanism involved in the coexistence of P.donghaiense and A.pacificum in the same area.
基金Research on in situ skin repair and regeneration based on micro-tissue engineering technology and 3D printing.(The National Key R&D Program of China,Grant Number 2019YFA0110503).The study on natural living micro-amniotic scaffolds to dynamic regulate immune inflammation and reconstruct wound repairing.(National Natural Science Foundation of China,Grant Number 81971836).The systemic study of miR-23b_24-1 cluster in the prevention and treatment of MODS caused by sepsis after burns.(National Natural Science Foundation of China,Grant Number 81930057).The experimental study on regulating the immune inflammatory microenvironment of burn wounds and promoting repair and regeneration based on micro-tissue engineering technology.(National Natural Science Foundation of China,Grant Number 81871559).
文摘Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rates.Negative pressure wound therapy(NPWT)is one of the most effective techniques for the treatment of diabetic foot wounds and great progress,both in terms of research and its clinical application,has been made in the last 20 years of its development.However,due to the complex pathogenesis and management of diabetic foot,irregular application of NPWT often leads to complications,such as infection,bleeding and necrosis,that seriously affect its treatment outcomes.In 2020,under the leadership of Burns,Trauma and Tissue Repair Committee of the Cross-Straits Medicine Exchange Association,the writing group for‘Consensus on the application of negative pressure wound therapy of diabetic foot wounds’was established with the participation of scholars from the specialized areas of burns,endocrinology,vascular surgery,orthopedics and wound repair.Drawing on evidence-based practice suggested by the latest clinical research,this consensus proposes the best clinical practice guidelines for the application and prognostic evaluation of NPWT for diabetic foot.The consensus aims to support the formation of standardized treatment schemes that clinicians can refer to when treating cases of diabetic foot.
文摘Science and technology talent evaluation is an important part of the talent development system and mechanism,and the state's emphasis on science and technology talent evaluation is reflect-ed in the national policy.This paper takes 49 policy documents on science and technology tal-ent evaluation issued by the state from 1978 to 2022 as the object,and uses policy text analysis,word frequency analysis and co-word network analysis to interpret the content of the policies.These policy texts are divided into four stages.200 high-frequency words were extracted from the policies in each stage after word segmentation was made,and the co-word matrixes and co-word networks were constructed for each stage.The research draws the following conclu-sions:1)The content of Chinese science and technology talent evaluation policies is gradually becoming concrete,and the concept of science and technology talent evaluation is gradually clear;2)The policies have transitioned from"scientific and technological development"to"sci-entific and technological innovation";3)The distribution of policies with different strengths is uneven;4)The cooperation between all departments involved in policy formulation is not e-nough.
基金supported by China Agriculture Research System(CARS-27)Research Programs for Nonprofit Technology and Application of Zhejiang Province,China(2009CB119203)。
文摘Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.
基金This work was supported by the earmarked fund for Modern Agro-industry Technology Research System(MATRS)of China,the National Foundation of Natural Science of China(31071649)The Global Research Network for Fungal Biology and King Saud University are thanked for supporting this research.MFLU awarded grant No 53101020017 to study the genus Phyllosticta in northern Thailand and the National Research Council of Thailand awarded grant No 54201020004 to study the genus Phyllosticta in Thailand.
文摘Phyllosticta species associated with diseases of four commercial Citrus species grown in China are reported.Totally,496 Phyllosticta strains were isolated from mandarins(Citrus reticulata),pomeloes(C.maxima),oranges(C.sinensis)and lemons(C.limon)in the main citrus producing regions across China,and 74 strains were selected for phylogenetic analysis.Analyses inferred from the sequences of internal transcribed spacer region(ITS1,5.8S nrDNA and ITS2),partial translation elongation factor 1-alpha(TEF1)and partial actin gene(ACT),showed these representative Phyllosticta isolates clustered in four distinct clades corresponding to three known,and one undescribed species.The newly resolved taxon,Phyllosticta citrichinaensis was isolated from leaves and fruits of all four Citrus species and is introduced in this paper.This taxon caused minor damage,showing irregular spots or freckles.Phyllosticta citriasiana,associated with tan spot of pomeloes,was isolated only from pomeloes,and never from lemons,mandarins and oranges.Phyllosticta citricarpa,the citrus black spot pathogen,which is presently subjected to phytosanitary legislation in the EU and United States,was isolated from lemons,mandarins and oranges,but never from pomeloes.The isolates of P.citricarpa clustered in two subclades,one from mandarins,the other from oranges and lemons.P.capitalensis was isolated from all four Citrus species as an endophyte,causing false melanose,or together with P.citricarpa or P.citriasiana.Morphological,cultural and biochemical characters were consistent with the results of phylogenetic analysis.In addition,a specific primer pair Pca8/ITS4 was designed and selected,and its corresponding PCR procedure was developed for the detection of P.citriasiana in this study.
基金supported by the National Natural Science Foundation of China(NSFC)(Nos.11974427 and 12004431)State Key Laboratory of Pulsed Power Laser(Nos.SKL-2020ZR05 and SKL2021ZR01)Postgraduate Scientific Research Innovation Project of Hunan Province(No.CX20200046)。
文摘We fabricate a pair of fiber Bragg gratings(FBGs)by a visible femtosecond laser phase mask scanning technique on passive large-mode-area double-cladding fibers for multi-kilowatt fiber oscillators.The bandwidth of high-reflection(HR)and lowreflection(LR)FBG is~1.6 nm and 0.3 nm,respectively.The reflection of the HR-FBG is higher than 99%,and that of the LR-FBG is about 10%.A bidirectional pumped all-fiber oscillator is constructed using this pair of FBGs,a record output power of 5027 W located in the signal core is achieved with a slope efficiency of~82.1%,and the beam quality factor M2is measured to be~1.6 at the maximum power.The FBGs are simply fixed on a water cooling plate without a special package,and the thermal efficiency of the HR-FBG and the LR-FBG is 2.76℃/kW and 1℃/kW,respectively.Our research provides an effective solution for robust high-power all-fiber laser oscillators.
文摘Side pumping combiners are widely used in fiber laser schemes for their high coupling efficiency, low insertion loss, and multi-point pumping capability. However, side pumping combiners perform differently in coupling efficiency when pumping with a laser diode(LD) and a high-brightness 1018 nm Yb-doped fiber laser(YDFL). In this paper, for the first time, to the best of our knowledge, we investigated the different parameters to fabricate the(2 + 1) × 1 combiner with high coupling efficiency when pumping with an LD and a YDFL, respectively. After optimization, the maximum coupled pump power from one single-pump port of the combiner was 1200 W and 2730 W when pumping with a LD and a YDFL, respectively.
基金the Out standing Youth Science Fund of Hunan Provincial Natural Science Foundation(No.2019JJ20023)the National Natural Science Foundation of China(NSFC)(No.11974427).
文摘We propose here a novel method for position fixing in the micron scale by combining the convolutional neural network(CNN) architecture and speckle patterns generated in a multimode fiber. By varying the splice offset between a single mode fiber and a multimode fiber, speckles with different patterns can be generated at the output of the multimode fiber. The CNN is utilized to learn these specklegrams and then predict the offset coordinate. Simulation results show that predicted positions with the precision of 2 μm account for 98.55%.This work provides a potential high-precision two-dimensional positioning method.
基金supported by the Guangdong Basic and Applied Basis Research Foundation(Grant No.2019B 1515120062).
文摘Microalgae harvesting remains a challenging step in microalgae industrialization,thereby provoking the necessity to explore sustainable and economically feasible approaches.This research investigated the use of magnetic flocculated nanoparticles in the harvesting of the common microalgae Chlorella pyrenoidosa and Scenedesmus obliquus.The results showed that magnetic flocculated nanoparticles efficiently adsorbed negatively charged microalgae cells,and a magnetic field could adsorb the magnetic flocculated nanoparticles,thereby harvesting the microalgae cells.Harvesting efficiency was remarkably increased at the optimum magnetic field strength of 0.5 T with the magnetic flocculated nanoparticles at 0.738 g/L,and microalgae broth at pH 9.0,whereas the recovery rates of both C.pyrenoidosa and S.obliquus were around 97%and the sedimentation speed of both was above 2.63 cm/min.This study exemplified the magnetic flocculated nanoparticles-based approach to effectively harvest the microalgae cells.