The microbial diversity in Wuli Area, Qinghai-Tibetan Plateau was investigated using 16S rRNA gene phylogenetic analyses. A total of 117 bacterial and 66 archaeal 16S rRNA gene clones were obtained from the Wuli cold ...The microbial diversity in Wuli Area, Qinghai-Tibetan Plateau was investigated using 16S rRNA gene phylogenetic analyses. A total of 117 bacterial and 66 archaeal 16S rRNA gene clones were obtained from the Wuli cold springs, The bacterial clones could be classified into Proteobacteria, Acid- obacteria, Deinococci, Sphingobacteria, Flavobacteria, Nitrospirae, Actinobacteria, Gemmatimona- detes, and unclassified-bacteria; and the archaeal clones could be classified into Crenarchaeota and Thaumarchaeota. Among the major groups, Proteobacteria and Crenarchaeota were dominant in the bacterial and archaeal 16S rRNA gene clone libraries, respectively. The clone sequences obtained in Wuli cold springs were closely related to those from cold habitats, such as snow/ice/soils on high mountains or at high latitude. Especially, the microbial community composition of Wuli Area was more similar to that in Tibetan glaciers than cold environments of other locations. The data presented in this study have impli- cations for a better understanding of microbial diversity in cold springs on the Qinghai-Tibetan Plateau.展开更多
基金supported by grants from National Science Foundation of China(Grant Nos.41030211 and 41002123)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Edncation Ministry,Research Fund for the Doctoral Program of Higher Education of China(Grant No. 20100022120009)+1 种基金the Fundamental Research Funds for the Central Universities(Grant Nos.2010ZY16 and 2011YXL03)the Teaching Laboratory Funds from China University of Geosciences,Beijing
文摘The microbial diversity in Wuli Area, Qinghai-Tibetan Plateau was investigated using 16S rRNA gene phylogenetic analyses. A total of 117 bacterial and 66 archaeal 16S rRNA gene clones were obtained from the Wuli cold springs, The bacterial clones could be classified into Proteobacteria, Acid- obacteria, Deinococci, Sphingobacteria, Flavobacteria, Nitrospirae, Actinobacteria, Gemmatimona- detes, and unclassified-bacteria; and the archaeal clones could be classified into Crenarchaeota and Thaumarchaeota. Among the major groups, Proteobacteria and Crenarchaeota were dominant in the bacterial and archaeal 16S rRNA gene clone libraries, respectively. The clone sequences obtained in Wuli cold springs were closely related to those from cold habitats, such as snow/ice/soils on high mountains or at high latitude. Especially, the microbial community composition of Wuli Area was more similar to that in Tibetan glaciers than cold environments of other locations. The data presented in this study have impli- cations for a better understanding of microbial diversity in cold springs on the Qinghai-Tibetan Plateau.
