Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant species.Employing molecular breeding technology for the development of cotton vari...Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant species.Employing molecular breeding technology for the development of cotton varieties with heightened resistance to VW stands out as one of the most efficacious protective measures.In this study,we successfully generated two stable transgenic lines of cotton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2,using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis confirmed the presence of a single-copy insertion in each line.Microscopic examination showed marked reductions in the colonization and spread of Vd-mCherry in the roots of VdThit-RNAi cotton compared to wild type(WT).The corresponding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant reductions.Real-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi cotton.Small RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic lines.Additionally,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in Vd.Under field conditions,VdThit-RNAi transgenic cotton exhibited significantly enhanced disease resistance and yield compared with WT.In summary,our findings underscore the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in cotton,thereby potentially enabling the development of cotton breeding as a promising strategy for managing VW.展开更多
Umbilical cord mesenchymal stem cells(UCMSCs)transplantation has been proposed as a promising treatment modality for myocardial infarction(MI),but the low retention rate remains a considerable challenge.Injectable nat...Umbilical cord mesenchymal stem cells(UCMSCs)transplantation has been proposed as a promising treatment modality for myocardial infarction(MI),but the low retention rate remains a considerable challenge.Injectable natural polymer hydrogels with conductivity ability are highly desirable as cell delivery vehicles to repair infarct myocardium and restore the cardiac function.In this work,we developed a hydrogel system based on gelatin methacrylate(GelMA)and oxidized dextran(ODEX)as cell delivery vehicles for MI.And dopamine could be used as a reductant of graphene oxide(GO)to form reductive GO(rGO).By adjusting the amount of rGO,the conductivity of hydrogels with 0.5 mg/mL rGO concentration(≈10^(-4)S/cm)was similar to that of natural heart tissue.In vitro cell experiments showed that the prepared hydrogels had excellent biocompatibility and cell delivery ability of UCMSCs.More importantly,GelMA-O5/rGO hydrogel could promote UCMSCs growth and proliferation,improve the myocardial differentiation ability of UCMSCs,and up-regulate the expression of cTnI and Cx43.Further in vivo experiments demonstrated that GelMA-O5/rGO/UCMSCs Hydrogel could significantly improve the ejection fraction(EF)of rats and significantly reduce myocardial infarct area compared to PBS group,promote the survival of UCMSCs,enhance the expression level of cTnI and Cx43,and decrease the expression level of caspase-3.The findings of this study suggested that the injectable conductive GelMA-O5/rGO hydrogel encapsulating UCMSCs could improve damaged myocardial tissue and reconstruct myocardial function,which will be a promising therapeutic strategy for cardiac repair.展开更多
Objective:This study aimed to compare the mood state of patients with severe pelvic organ prolapse(POP)before and after surgery.Methods:This prospective study enrolled 100 patients with severe POP who were surgically ...Objective:This study aimed to compare the mood state of patients with severe pelvic organ prolapse(POP)before and after surgery.Methods:This prospective study enrolled 100 patients with severe POP who were surgically treated between October 2016 and February 2019.The clinical severity of POP was evaluated using the POP Quantification(POPQ)System.Mood state evaluation,using the Hamilton anxiety(HAMA)and the Hamilton depression(HAMD)scale,was performed preoperatively and at 3 and 6 months postoperatively.Results:The POP-Q stage was significantly improved postoperatively(P<0.05).Preoperatively,the patients had varying degrees of anxiety symptoms(mean HAMA score,11.945.59 points)and depression symptoms(mean HAMD score,5.562.58 points);these scores were significantly decreased at 3 months(mean HAMA score,4.672.02 points;mean HAMD score,3.581.61 points)and 6 months postoperatively(mean HAMA score,4.882.57 points;mean HAMD score,3.381.60 points)(P<0.05).There were no differences in the HAMD and HAMA scores in both traditional prolapse surgery patients and mesh pelvic reconstruction patients preoperatively and at 3 and 6 months postoperatively(P>0.05).Conclusions:Patients with severe POP have different anxiety and depression symptoms preoperatively.Successful individualized surgical treatment is effective for improving the clinical severity of severe POP and might alleviate or improve POP-related anxiety or depression symptoms.展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200300)the National Natural Science Foundation of China(32072376 and 32372515)+3 种基金Winall Hi-tech Seed Co.,Ltd.,China(GMLM2023)the Nanfan Special Project of Chinese Academy of Agricultural Sciences(CAAS)(ZDXM2303 and YBXM2415)the Natural Science Foundation of Hebei Province,China(C2022204205)the Agricultural Science and Technology Innovation Program of CAAS。
文摘Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant species.Employing molecular breeding technology for the development of cotton varieties with heightened resistance to VW stands out as one of the most efficacious protective measures.In this study,we successfully generated two stable transgenic lines of cotton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2,using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis confirmed the presence of a single-copy insertion in each line.Microscopic examination showed marked reductions in the colonization and spread of Vd-mCherry in the roots of VdThit-RNAi cotton compared to wild type(WT).The corresponding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant reductions.Real-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi cotton.Small RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic lines.Additionally,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in Vd.Under field conditions,VdThit-RNAi transgenic cotton exhibited significantly enhanced disease resistance and yield compared with WT.In summary,our findings underscore the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in cotton,thereby potentially enabling the development of cotton breeding as a promising strategy for managing VW.
基金This work was supported by National Key Research and Development Program of China(2018YFA0108700,2017YFA0105602)NSFC Projects of International Cooperation and Exchanges(81720108004)+3 种基金National Natural Science Foundation of China(81974019)the Research Team Project of Natural Science Foundation of Guangdong Province of China(2017A030312007)the key program of guangzhou science research plan(201904020047)the Special Project of Dengfeng Program of Guangdong Provincial People’s Hospital(DFJH201812,KJ012019119,KJ012019423).
文摘Umbilical cord mesenchymal stem cells(UCMSCs)transplantation has been proposed as a promising treatment modality for myocardial infarction(MI),but the low retention rate remains a considerable challenge.Injectable natural polymer hydrogels with conductivity ability are highly desirable as cell delivery vehicles to repair infarct myocardium and restore the cardiac function.In this work,we developed a hydrogel system based on gelatin methacrylate(GelMA)and oxidized dextran(ODEX)as cell delivery vehicles for MI.And dopamine could be used as a reductant of graphene oxide(GO)to form reductive GO(rGO).By adjusting the amount of rGO,the conductivity of hydrogels with 0.5 mg/mL rGO concentration(≈10^(-4)S/cm)was similar to that of natural heart tissue.In vitro cell experiments showed that the prepared hydrogels had excellent biocompatibility and cell delivery ability of UCMSCs.More importantly,GelMA-O5/rGO hydrogel could promote UCMSCs growth and proliferation,improve the myocardial differentiation ability of UCMSCs,and up-regulate the expression of cTnI and Cx43.Further in vivo experiments demonstrated that GelMA-O5/rGO/UCMSCs Hydrogel could significantly improve the ejection fraction(EF)of rats and significantly reduce myocardial infarct area compared to PBS group,promote the survival of UCMSCs,enhance the expression level of cTnI and Cx43,and decrease the expression level of caspase-3.The findings of this study suggested that the injectable conductive GelMA-O5/rGO hydrogel encapsulating UCMSCs could improve damaged myocardial tissue and reconstruct myocardial function,which will be a promising therapeutic strategy for cardiac repair.
文摘Objective:This study aimed to compare the mood state of patients with severe pelvic organ prolapse(POP)before and after surgery.Methods:This prospective study enrolled 100 patients with severe POP who were surgically treated between October 2016 and February 2019.The clinical severity of POP was evaluated using the POP Quantification(POPQ)System.Mood state evaluation,using the Hamilton anxiety(HAMA)and the Hamilton depression(HAMD)scale,was performed preoperatively and at 3 and 6 months postoperatively.Results:The POP-Q stage was significantly improved postoperatively(P<0.05).Preoperatively,the patients had varying degrees of anxiety symptoms(mean HAMA score,11.945.59 points)and depression symptoms(mean HAMD score,5.562.58 points);these scores were significantly decreased at 3 months(mean HAMA score,4.672.02 points;mean HAMD score,3.581.61 points)and 6 months postoperatively(mean HAMA score,4.882.57 points;mean HAMD score,3.381.60 points)(P<0.05).There were no differences in the HAMD and HAMA scores in both traditional prolapse surgery patients and mesh pelvic reconstruction patients preoperatively and at 3 and 6 months postoperatively(P>0.05).Conclusions:Patients with severe POP have different anxiety and depression symptoms preoperatively.Successful individualized surgical treatment is effective for improving the clinical severity of severe POP and might alleviate or improve POP-related anxiety or depression symptoms.
