Soil shrinkage is an important factor in slope destabilization in granitic areas, which is also one of the most important conditions for the formation of permanent gullies. This study explored the effect of soil shrin...Soil shrinkage is an important factor in slope destabilization in granitic areas, which is also one of the most important conditions for the formation of permanent gullies. This study explored the effect of soil shrinkage on permanent gullies, and Benggang erosion in granitic areas in southeastern China was used as an example. Three types of Benggang in granitic area were selected to examine the soil shrinkage of three soil layers(the lateritic, transitional and sandy layers) and their effect on the development of Benggang erosion was studied. The results show that the maximum values of COLEH and COLEV(coefficient of linear extensibility in horizontal and vertical directions, respectively) are 3.09% and 1.60% in the laterite layers, 2.71% and 2.13% in transition layers, and 1.10% and 1.82% in sandy layers, indicating that the shrinkage potential of the soil layers exhibits the following order, from highest to lowest: the laterite layer, transition layer, and sandy layer. With a decreasing volumetric water content, the linear shrinkage ratio increases gradually and eventually stabilizes, and in the laterite, transition and sandy layers, the average values of the maximum linear shrinkage are 1.50%, 2.09%, and 1.74%, respectively. Axial shrinkage is most obvious in the transition layers, in which the volume change in the form of subsidence is greater than that in other layers. The soil shrinkage curves fit the trilinear model(R2>0.9), and the soil shrinkage characteristic curves were divided into structural, basic, and residual sections. The correlation analysis shows that the soil shrinkage rate is positively correlated with clay and Fe2 O3 content and negatively correlated with sand content. Clay and sand contents are the most important factors influencing soil shrinkage. Soil oxides can influence soil shrinkage by affecting the particle composition of the soil, so soil shrinkage is closely related to soil clay minerals. Our findings can provide a theoretical basis for revealing the mechanism of Benggang erosion and its control.展开更多
Polymerase chain reaction （PCR） was used to amplify a 600-base pair （bp） sequence of plasmid pGEX-2T DNA bound on soil colloidal particles from Brown soil （Alfisol） and Red soil （Ultisol）, and three different ...Polymerase chain reaction （PCR） was used to amplify a 600-base pair （bp） sequence of plasmid pGEX-2T DNA bound on soil colloidal particles from Brown soil （Alfisol） and Red soil （Ultisol）, and three different minerals （goethite, kaolinite, montmorillonite）. DNA bound on soil colloids, kaolinite, and montmorillonite was not amplified when the complexes were used directly but amplification occurred when the soil colloid or kaolinite-DNA complex was diluted, 10- and 20-fold. The montmorillonite-DNA complex required at least 100-fold dilution before amplification could be detected. DNA bound on goethite was amplified irrespective of whether the complex was used directly, or diluted 10- and 20-fold. The amplification of mineral-bound plasmid DNA by PCR is, therefore, markedly influenced by the type and concentration of minerals used. This information is of fundamental importance to soil molecular microbial ecology with particular reference to monitoring the fate of genetically engineered microorganisms and their recombinant DNA in soil environments.展开更多
基金This study was supported by the National Natural Science Foundation of China,(Grant No.42007055,41630858)。
文摘Soil shrinkage is an important factor in slope destabilization in granitic areas, which is also one of the most important conditions for the formation of permanent gullies. This study explored the effect of soil shrinkage on permanent gullies, and Benggang erosion in granitic areas in southeastern China was used as an example. Three types of Benggang in granitic area were selected to examine the soil shrinkage of three soil layers(the lateritic, transitional and sandy layers) and their effect on the development of Benggang erosion was studied. The results show that the maximum values of COLEH and COLEV(coefficient of linear extensibility in horizontal and vertical directions, respectively) are 3.09% and 1.60% in the laterite layers, 2.71% and 2.13% in transition layers, and 1.10% and 1.82% in sandy layers, indicating that the shrinkage potential of the soil layers exhibits the following order, from highest to lowest: the laterite layer, transition layer, and sandy layer. With a decreasing volumetric water content, the linear shrinkage ratio increases gradually and eventually stabilizes, and in the laterite, transition and sandy layers, the average values of the maximum linear shrinkage are 1.50%, 2.09%, and 1.74%, respectively. Axial shrinkage is most obvious in the transition layers, in which the volume change in the form of subsidence is greater than that in other layers. The soil shrinkage curves fit the trilinear model(R2>0.9), and the soil shrinkage characteristic curves were divided into structural, basic, and residual sections. The correlation analysis shows that the soil shrinkage rate is positively correlated with clay and Fe2 O3 content and negatively correlated with sand content. Clay and sand contents are the most important factors influencing soil shrinkage. Soil oxides can influence soil shrinkage by affecting the particle composition of the soil, so soil shrinkage is closely related to soil clay minerals. Our findings can provide a theoretical basis for revealing the mechanism of Benggang erosion and its control.
基金Project supported by the National Natural Science Foundation of China(No.40271064)
文摘Polymerase chain reaction （PCR） was used to amplify a 600-base pair （bp） sequence of plasmid pGEX-2T DNA bound on soil colloidal particles from Brown soil （Alfisol） and Red soil （Ultisol）, and three different minerals （goethite, kaolinite, montmorillonite）. DNA bound on soil colloids, kaolinite, and montmorillonite was not amplified when the complexes were used directly but amplification occurred when the soil colloid or kaolinite-DNA complex was diluted, 10- and 20-fold. The montmorillonite-DNA complex required at least 100-fold dilution before amplification could be detected. DNA bound on goethite was amplified irrespective of whether the complex was used directly, or diluted 10- and 20-fold. The amplification of mineral-bound plasmid DNA by PCR is, therefore, markedly influenced by the type and concentration of minerals used. This information is of fundamental importance to soil molecular microbial ecology with particular reference to monitoring the fate of genetically engineered microorganisms and their recombinant DNA in soil environments.
