Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheime...Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.展开更多
We propose an efficient scheme to produce ultrahigh-brightness tens of MeV electron beams by designing a density-tailored plasma to induce a wakefield in the weakly nonlinear regime with a moderate laser energy of 120...We propose an efficient scheme to produce ultrahigh-brightness tens of MeV electron beams by designing a density-tailored plasma to induce a wakefield in the weakly nonlinear regime with a moderate laser energy of 120 mJ.In this scheme,the second bucket of the wakefield can have a much lower phase velocity at the steep plasma density down-ramp than the first bucket and can be exploited to implement longitudinal electron injection at a lower laser intensity,leading to the generation of bright electron beams with ultralow emittance together with low energy spread.Three-dimensional particle-in-cell simulations are carried out and demonstrate that high-quality electron beams with a peak energy of 50 MeV,ultralow emittance of28 nm rad,energy spread of 1%,charge of 4.4 pC,and short duration less than 5 fs can be obtained within a 1-mm-long tailored plasma density,resulting in an ultrahigh six-dimensional brightness B6D,n of2×1017 A/m2/0.1%.By changing the density parameters,tunable bright electron beams with peak energies ranging from 5 to 70 MeV,a small emittance of B0.1 mm mrad,and a low energy spread at a few-percent level can be obtained.These bright MeV-class electron beams have a variety of potential applications,for example,as ultrafast electron probes for diffraction and imaging,in laboratory astrophysics,in coherent radiation source generation,and as injectors for GeV particle accelerators.展开更多
To date,the benchmark Bi_(2)Te_(3)-based alloys are still the only commercial material system used for ther-moelectric solid-state refrigeration.Nonetheless,the conspicuous performance imbalance between the p-type Bi_...To date,the benchmark Bi_(2)Te_(3)-based alloys are still the only commercial material system used for ther-moelectric solid-state refrigeration.Nonetheless,the conspicuous performance imbalance between the p-type Bi_(2-x)Sb_(x)Te_(3)and n-type Bi_(2)Te_(3-x)Se_(x) legs has become a major obstacle for the improvement of cooling devices to achieve higher efficiency.In our previous study,novel n-type Bi_(2-x)Sb_(x)Te_(3)alloy has been pro-posed via manipulating donor-like effect as an alternative to mainstream n-type Bi_(2)Te_(3-x)Se_(x).However,the narrow bandgap of Bi_(2-x)Sb_(x)Te_(3)provoked severe bipolar effect that constrained the further improvement of zT near room temperature.Herein,we have implemented band gap engineering in n-type Bi_(1.5)Sb_(0.5)Te_(3)by employing isovalent Se substitution to inhibit the undesired intrinsic excitation and achieve the dis-tinguished room-temperature zT.First,the preferential occupancy of Se at Te^(2)site appropriately enlarges the band gap,thereby concurrently improving the Seebeck coefficient and depressing the bipolar thermal conductivity.In addition,the Se alloying mildly suppresses the compensation mechanism and essentially preserves the already optimized carrier concentration,which maintains the peak zT near room tempera-ture.Moreover,the large strain field and mass fluctuation generated by Se alloying leads to the remark-able reduction of lattice thermal conductivity.Accordingly,the zT value of Bi_(1.5)Sb_(0.5)Te_(2.8)Se_(0.2)reaches 1.0 at 300 K and peaks 1.1 at 360 K,which surpasses that of most well-known room-temperature n-type thermoelectric materials.These results pave the way for n-type Bi_(2-x)Sb_(x)Te_(3)alloys to become a new and promising top candidate for large-scale solid-state cooling applications.展开更多
Soft robotics has several promising properties for aquatic applications, such as safe interaction with environments, lightweight, low cost, etc. In this paper, we proposed the kinematic modeling and hydrodynamics expe...Soft robotics has several promising properties for aquatic applications, such as safe interaction with environments, lightweight, low cost, etc. In this paper, we proposed the kinematic modeling and hydrodynamics experiments of a soft robotic arm with 3D locomotion capacity. We developed a mathematical model that incorporates the angle correction, as well as the open-loop model-based motion control. The model could precisely predict the three-dimensional （3D） movement, and the location error is less than 5.7 mm in different attitudes. Furthermore, we performed the hydrodynamic investigations and simultaneously measured the hydrodynamic forces and the wake flows at different amplitudes （50 mm, 100 mm, 150 mm, 200 mm） and frequencies （0.3 Hz, 0.4 Hz, 0.5 Hz） of the soft arm. Surprisingly, we found that the magnitudes of the hydrodynamic force （〈1 N） and the torques （〈0.08 N-m） of dynamically moving soft arm were tiny, which leads to negligible inertial effect for the underwater vehicle than those of the traditional rigid underwater manipulator. Finally, we demonstrated underwater picking and placing tasks of the soft manipulator by using a computer program that controls the tip attitude and velocity. This study may inspire future underwater manipulators that have properties of low-inertial, low power cost and can safely interact with the aauatic environments.展开更多
In recent years, luminescent metal-organic frameworks （MOFs） as a new type of sensing material are receiving enormous attention for their superior performance in chemical sensors and biosensors [1-2]. Taking account...In recent years, luminescent metal-organic frameworks （MOFs） as a new type of sensing material are receiving enormous attention for their superior performance in chemical sensors and biosensors [1-2]. Taking account of the excellent optical properties such as large Stokes shifts and high color purity of lanthanide MOFs （LnMOFs）, a great deal of important investigations on LnMOFs have been carried out. And then, their promising abilities in detecting temperature, metal ions, oxygen, explosives and polychlorizated benzenes with high sensitivity and se- lectivity have also been exploited successfuUy [3-22]. Ratiometric luminescent sensors can provide a self-cali- brated analyte concentration readout, which is unaffected by fluctuations of sensor concentration and/or instru- mental parameters. However, it is noticed that a majority of related reports are limited to the detection of analyte using single emission, although ratiometric sensors based on dual-emission are more reliable and accurate than the sensors based on single emission. For ratiometric sensors, not only are two significantly different emissions neces- sary, but two emissions need distinctive response to the analyte. From this point of view, LnMOFs with ligand and Ln3＋ emissions are very attractive, because organic ligands and Ln3＋ ions as the luminescent centers possess completely different physical and chemical properties and they would produce different interactions with analytes. Thus, LnMOFs have been extensively applied to construct ratiometric sensors recently [3,22].展开更多
A metal-organic framework [Eu_3L_3(CH_3COO)_2(H_2O)_2(μ_3-OH)]·3 DMF,(EuL, H_2L=9H-carbazole-2,7-dicarboxylic acid,DMF=N,N-dimethylformamide) has been synthesized under solvothermal conditions and structurally c...A metal-organic framework [Eu_3L_3(CH_3COO)_2(H_2O)_2(μ_3-OH)]·3 DMF,(EuL, H_2L=9H-carbazole-2,7-dicarboxylic acid,DMF=N,N-dimethylformamide) has been synthesized under solvothermal conditions and structurally characterized. In EuL,Eu_6O_8 clusters are four-bridged by carboxylates to form parallel-aligned Eu–O–C chains, which are further linked by the carbazole moieties of L^(2-) ligands to form the three-dimensional framework with rhombic channels. The EuL material with characteristic emission of Eu^(3+) ion exhibits significant luminescence quenching response for picric acid(PA) and the linear Stern-Volmer plot was observed in the concentration range of 0.05–0.15 mM with K_(sv) of 98074 M^(-1). As far as we know, this Ksv is among the highest values for COFs and MOFs in detection of PA. The excellent anti-interference ability and repeatability were also verified by experiments. Lastly, we investigated the luminescence quenching mechanism in the EuL sensing system.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82171194 and 81974155(both to JL)the Shanghai Municipal Science and Technology Commission Medical Guide Project,No.16411969200(to WZ)Shanghai Municipal Science and Technology Commission Biomedical Science and Technology Project,No.22S31902600(to JL)。
文摘Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.11974251,12105180,12074397,11904377,and 12005137)the Innovation Program of Shanghai Municipal Education Commission(Grant No.2021-01-07-00-02-E00118)the National Key Research and Development Program(Grant No.2023YFA1406804).
文摘We propose an efficient scheme to produce ultrahigh-brightness tens of MeV electron beams by designing a density-tailored plasma to induce a wakefield in the weakly nonlinear regime with a moderate laser energy of 120 mJ.In this scheme,the second bucket of the wakefield can have a much lower phase velocity at the steep plasma density down-ramp than the first bucket and can be exploited to implement longitudinal electron injection at a lower laser intensity,leading to the generation of bright electron beams with ultralow emittance together with low energy spread.Three-dimensional particle-in-cell simulations are carried out and demonstrate that high-quality electron beams with a peak energy of 50 MeV,ultralow emittance of28 nm rad,energy spread of 1%,charge of 4.4 pC,and short duration less than 5 fs can be obtained within a 1-mm-long tailored plasma density,resulting in an ultrahigh six-dimensional brightness B6D,n of2×1017 A/m2/0.1%.By changing the density parameters,tunable bright electron beams with peak energies ranging from 5 to 70 MeV,a small emittance of B0.1 mm mrad,and a low energy spread at a few-percent level can be obtained.These bright MeV-class electron beams have a variety of potential applications,for example,as ultrafast electron probes for diffraction and imaging,in laboratory astrophysics,in coherent radiation source generation,and as injectors for GeV particle accelerators.
基金supported by the National Natural Science Foundation of China(52071218)Shenzhen Science and Technology Innovation Commission(20200731215211001 and 20200814110413001)Guangdong Basic and Applied Basic Research Foundation(2022A1515012492)。
基金The work is supported by the National Natural Science Foundation of China(No.52071218)Shenzhen Science and Technology Innovation Commission(Nos.20200731215211001,20200814110413001)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515012492).The authors also appreciate the Instrumental Analysis Center of Shenzhen University.
文摘To date,the benchmark Bi_(2)Te_(3)-based alloys are still the only commercial material system used for ther-moelectric solid-state refrigeration.Nonetheless,the conspicuous performance imbalance between the p-type Bi_(2-x)Sb_(x)Te_(3)and n-type Bi_(2)Te_(3-x)Se_(x) legs has become a major obstacle for the improvement of cooling devices to achieve higher efficiency.In our previous study,novel n-type Bi_(2-x)Sb_(x)Te_(3)alloy has been pro-posed via manipulating donor-like effect as an alternative to mainstream n-type Bi_(2)Te_(3-x)Se_(x).However,the narrow bandgap of Bi_(2-x)Sb_(x)Te_(3)provoked severe bipolar effect that constrained the further improvement of zT near room temperature.Herein,we have implemented band gap engineering in n-type Bi_(1.5)Sb_(0.5)Te_(3)by employing isovalent Se substitution to inhibit the undesired intrinsic excitation and achieve the dis-tinguished room-temperature zT.First,the preferential occupancy of Se at Te^(2)site appropriately enlarges the band gap,thereby concurrently improving the Seebeck coefficient and depressing the bipolar thermal conductivity.In addition,the Se alloying mildly suppresses the compensation mechanism and essentially preserves the already optimized carrier concentration,which maintains the peak zT near room tempera-ture.Moreover,the large strain field and mass fluctuation generated by Se alloying leads to the remark-able reduction of lattice thermal conductivity.Accordingly,the zT value of Bi_(1.5)Sb_(0.5)Te_(2.8)Se_(0.2)reaches 1.0 at 300 K and peaks 1.1 at 360 K,which surpasses that of most well-known room-temperature n-type thermoelectric materials.These results pave the way for n-type Bi_(2-x)Sb_(x)Te_(3)alloys to become a new and promising top candidate for large-scale solid-state cooling applications.
基金Acknowledgment We thank Yufei Hao and Guangyao Huang for their help on this work. This work was supported by the National Science Foundation support key projects, China, under contract numbers 61633004 and 61333016.
文摘Soft robotics has several promising properties for aquatic applications, such as safe interaction with environments, lightweight, low cost, etc. In this paper, we proposed the kinematic modeling and hydrodynamics experiments of a soft robotic arm with 3D locomotion capacity. We developed a mathematical model that incorporates the angle correction, as well as the open-loop model-based motion control. The model could precisely predict the three-dimensional （3D） movement, and the location error is less than 5.7 mm in different attitudes. Furthermore, we performed the hydrodynamic investigations and simultaneously measured the hydrodynamic forces and the wake flows at different amplitudes （50 mm, 100 mm, 150 mm, 200 mm） and frequencies （0.3 Hz, 0.4 Hz, 0.5 Hz） of the soft arm. Surprisingly, we found that the magnitudes of the hydrodynamic force （〈1 N） and the torques （〈0.08 N-m） of dynamically moving soft arm were tiny, which leads to negligible inertial effect for the underwater vehicle than those of the traditional rigid underwater manipulator. Finally, we demonstrated underwater picking and placing tasks of the soft manipulator by using a computer program that controls the tip attitude and velocity. This study may inspire future underwater manipulators that have properties of low-inertial, low power cost and can safely interact with the aauatic environments.
基金supported by the National Natural Science Foundation of China (21271143)Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) (YX03001)+1 种基金Jiangsu Province Double Innovation Talent Program (090300014001)Nanjing University of Posts & Telecommunications (NY212004)
文摘In recent years, luminescent metal-organic frameworks （MOFs） as a new type of sensing material are receiving enormous attention for their superior performance in chemical sensors and biosensors [1-2]. Taking account of the excellent optical properties such as large Stokes shifts and high color purity of lanthanide MOFs （LnMOFs）, a great deal of important investigations on LnMOFs have been carried out. And then, their promising abilities in detecting temperature, metal ions, oxygen, explosives and polychlorizated benzenes with high sensitivity and se- lectivity have also been exploited successfuUy [3-22]. Ratiometric luminescent sensors can provide a self-cali- brated analyte concentration readout, which is unaffected by fluctuations of sensor concentration and/or instru- mental parameters. However, it is noticed that a majority of related reports are limited to the detection of analyte using single emission, although ratiometric sensors based on dual-emission are more reliable and accurate than the sensors based on single emission. For ratiometric sensors, not only are two significantly different emissions neces- sary, but two emissions need distinctive response to the analyte. From this point of view, LnMOFs with ligand and Ln3＋ emissions are very attractive, because organic ligands and Ln3＋ ions as the luminescent centers possess completely different physical and chemical properties and they would produce different interactions with analytes. Thus, LnMOFs have been extensively applied to construct ratiometric sensors recently [3,22].
基金supported by the National Natural Science Foundation of China (61575096)Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) (YX03001)+2 种基金Jiangsu Province Double Innovation Talent Program (090300014001)Nanjing University of Posts & Telecommunications (NY212004, NY217074)Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX17_0748 and KYCX18_0857)
文摘A metal-organic framework [Eu_3L_3(CH_3COO)_2(H_2O)_2(μ_3-OH)]·3 DMF,(EuL, H_2L=9H-carbazole-2,7-dicarboxylic acid,DMF=N,N-dimethylformamide) has been synthesized under solvothermal conditions and structurally characterized. In EuL,Eu_6O_8 clusters are four-bridged by carboxylates to form parallel-aligned Eu–O–C chains, which are further linked by the carbazole moieties of L^(2-) ligands to form the three-dimensional framework with rhombic channels. The EuL material with characteristic emission of Eu^(3+) ion exhibits significant luminescence quenching response for picric acid(PA) and the linear Stern-Volmer plot was observed in the concentration range of 0.05–0.15 mM with K_(sv) of 98074 M^(-1). As far as we know, this Ksv is among the highest values for COFs and MOFs in detection of PA. The excellent anti-interference ability and repeatability were also verified by experiments. Lastly, we investigated the luminescence quenching mechanism in the EuL sensing system.
