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Plutonium utilization in a small modular molten-salt reactor based on a batch fuel reprocessing scheme
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作者 Xue-Chao Zhao Rui Yan +4 位作者 Gui-Feng Zhu Ya-Fen Liu jian guo Xiang-Zhou Cai Yang Zou 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第4期15-28,共14页
A molten salt reactor(MSR)has outstanding features considering the application of thorium fuel,inherent safety,sustainability,and resistance to proliferation.However,fissile material^(233)U is significantly rare at th... A molten salt reactor(MSR)has outstanding features considering the application of thorium fuel,inherent safety,sustainability,and resistance to proliferation.However,fissile material^(233)U is significantly rare at the current stage,thus it is difficult for MSR to achieve a pure thorium-uranium fuel cycle.Therefore,using plutonium or enriched uranium as the initial fuel for MSR is more practical.In this study,we aim to verify the feasibility of a small modular MSR that utilizes plutonium as the starting fuel(SM-MSR-Pu),and highlight its advantages and disadvantages.First,the structural design and fuel management scheme of the SM-MSR-Pu were presented.Second,the neutronic characteristics,such as the graphite-irradiation lifetime,burn-up performance,and coefficient of temperature reactivity were calculated to analyze the physical characteristics of the SM-MSR-Pu.The results indicate that plutonium is a feasible and advantageous starting fuel for a SM-MSR;however,there are certain shortcomings that need to be solved.In a 250 MWth SM-MSR-Pu,approximately 288.64 kg^(233)U of plutonium with a purity of greater than 90% is produced while 978.00 kg is burned every ten years.The temperature reactivity coefficient decreases from -4.0 to -6.5 pcm K^(-1) over the 50-year operating time,which ensures a long-term safe operation.However,the amount of plutonium and accumulation of minor actinides(MAs)would increase as the burn-up time increases,and the annual production and purity of^(233)U will decrease.To achieve an optimal burn-up performance,setting the entire operation time to 30 years is advisable.Regardless,more than 3600 kg of plutonium eventually accumulate in the core.Further research is required to effectively utilize this accumulated plutonium. 展开更多
关键词 Molten salt fuel Plutonium utilization ^(233)U TRUs mole fraction Temperature feedback coefficient
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Impaired pericyte-Müller glia interaction via PDGFRβ suppression aggravates photoreceptor loss in a rodent model of light-induced retinal injury
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作者 Wei Xu Li-Jin Cui +3 位作者 Xiao-Ying Yang Xiao-Yuan Cui jian guo guo-Xing Xu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第10期1800-1808,共9页
AIM:To investigate the involvement of pericyte-Müller glia interaction in retinal damage repair and assess the influence of suppressing the platelet-derived growth factor receptorβ(PDGFRβ)signaling pathway in r... AIM:To investigate the involvement of pericyte-Müller glia interaction in retinal damage repair and assess the influence of suppressing the platelet-derived growth factor receptorβ(PDGFRβ)signaling pathway in retinal pericytes on photoreceptor loss and Müller glial response.METHODS:Sprague-Dawley rats were exposed to intense light to induce retinal injury.Neutralizing antibody against PDGFRβwere deployed to block the signaling pathway in retinal pericytes through intravitreal injection.Retinal histology and Müller glial reaction were assessed following light injury.In vitro,normal and PDGFRβ-blocked retinal pericytes were cocultured with Müller cell line(rMC-1)to examine morphological and protein expression changes upon supplementation with light-injured supernatants of homogenized retinas(SHRs).RESULTS:PDGFRβblockage 24h prior to intense light exposure resulted in a significant exacerbation of photoreceptor loss.The upregulation of GFAP and p-STAT3,observed after intense light exposure,was significantly inhibited in the PDGFRβblockage group.Fur ther upregulation of cytokines monocyte chemoattractant protein 1(MCP-1)and interleukin-1β(IL-1β)was also observed following PDGFRβinhibition.In the in vitro coculture system,the addition of light-injured SHRs induced pericyte deformation and upregulation of proliferating cell nuclear antigen(PCNA)expression,while Müller cells exhibited neuron-like morphology and expressed Nestin.However,PDGFRβblockage in retinal pericytes abolished these cellular responses to light-induced damage,consistent with the in vivo PDGFRβblockage findings.CONCLUSION:Pericyte-Müller glia interaction plays a potential role in the endogenous repair process of retinal injury.Impairment of this interaction exacerbates photoreceptor degeneration in light-induced retinal injury. 展开更多
关键词 PERICYTE Müller glia light-induced retinal injury platelet-derived growth factor receptorβ signal pathway
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Study on Evacuation Strategy of Commercial High-Rise Building under Fire Based on FDS and Pathfinder
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作者 Zheng Yan Ying Wang +1 位作者 Longxiao Chao jian guo 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第2期1077-1102,共26页
With the development of economy and society and the growth of population,the high-rise and multi-function of commercial buildings have become an international trend.But it also poses huge fire hazards.Most of the exis... With the development of economy and society and the growth of population,the high-rise and multi-function of commercial buildings have become an international trend.But it also poses huge fire hazards.Most of the existing studies’research objects are predominantly high-rise residential buildings,without considering the impact of different functional zones(Standard floor,entertainment zone,office zone,equipment room and so on)and personnel distribution of commercial buildings evacuation.And the influence of using elevators to carry evacuees on the refuge floor on personnel evacuation is rarely studied.In this work,the fire scenario of the Yangtze River InternationalConferenceCenter,a high-rise commercial building,is simulated with the Pyrosim programto get the necessary parameters under various fire scenarios and to calculate the available evacuation time TASET.At the same time,according to the complex functional zone of the commercial high-rise building and the distribution of people in different time periods,a reasonable evacuation strategy is developed and simulated by Pathfinder software.The results indicate that unorganized evacuation will lead individuals to take the erroneous evacuation route,resulting in a vast region of congestion;comprehensive consideration of the time staggering and the reasonable distribution of evacuation routes can significantly improve evacuation efficiency,and the TRSET of night and working hours is 36.6%–55.3%and 49.9%–79.6%of unorganized evacuation,respectively.For the night fire,60%of the people use elevator-refuge floor to evacuate is the optimal strategy;for the fire during working hours,half of the people on standard floors use the elevator to evacuate and people on multifunctional floors evacuate in four batches is the best plan.The results of this study can provide viable solutions and a foundation for analyzing the fire evacuation and safety of big commercial high-rise buildings. 展开更多
关键词 High-rise building fire personnel evacuation refuge floor safety analysis
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MgO对赤铁矿焙烧压团组成结构与强度的影响 被引量:3
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作者 朱建国 孙晨晨 +1 位作者 罗果萍 王永斌 《有色金属(冶炼部分)》 CAS 北大核心 2018年第10期5-9,共5页
在赤铁矿中添加MgO制备焙烧压团,利用矿相显微分析、扫描电镜分析、抗压强度检测和FactSage热力学计算相结合的方法,研究了MgO添加量对焙烧压团组成结构与抗压强度的影响机理。结果表明,随着MgO的增加,压团孔隙率降低较为明显;焙烧压团... 在赤铁矿中添加MgO制备焙烧压团,利用矿相显微分析、扫描电镜分析、抗压强度检测和FactSage热力学计算相结合的方法,研究了MgO添加量对焙烧压团组成结构与抗压强度的影响机理。结果表明,随着MgO的增加,压团孔隙率降低较为明显;焙烧压团未氧化的磁铁矿含量升高,赤铁矿含量降低,且随着MgO添加量增加,焙烧压团具有很好的综合显微力学性能,在压团氧化焙烧过程中,应适当增加磁铁矿的含量,以增加压团抗压强度;当MgO添加量为2%和4%时,焙烧压团磁铁矿含量分别达到18.97%和24.16%,在不配加粘结剂的情况下,每个压团抗压强度可以达到1.924kN和1.993kN。 展开更多
关键词 MGO 赤铁矿 磁铁矿 结构 抗压强度
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低成本小型无线自动太阳敏感器设计与实现 被引量:5
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作者 冯邈 于晓洲 +2 位作者 白博 周军 jian guo 《系统工程与电子技术》 EI CSCD 北大核心 2019年第8期1852-1857,共6页
针对微小卫星姿态确定与控制系统的需求,西北工业大学与荷兰代尔夫特理工大学共同研发了低成本小型无线自动太阳敏感器(Micro Wireless Automatic Sun Sensor,μWASS)。该敏感器采用四象限探测器作为感光部件,使用集成ZigBee通信协议的... 针对微小卫星姿态确定与控制系统的需求,西北工业大学与荷兰代尔夫特理工大学共同研发了低成本小型无线自动太阳敏感器(Micro Wireless Automatic Sun Sensor,μWASS)。该敏感器采用四象限探测器作为感光部件,使用集成ZigBee通信协议的低功耗51单片机内核作为处理器,利用三结砷化镓太阳能电池片产生电能为系统供电。在能量储存方面,创造性地使用了超级电容作为储能部件。同时,在系统设计中考虑了空间环境辐射与高低温变化,增加了额外保护电路。μWASS视场角为120°×120°,在整个视场范围内精度小于0.5°(1σ),平均功耗为30 mW。与目前现有的模拟式太阳敏感器相比,μWASS的测量数据通过无线通信方式传输,不需要专门接插件以及外部模拟/数字转换电路,可以实现即插即用,降低了太阳敏感器在卫星上的安装难度,提高了系统可靠性。μWASS实现了太阳敏感器的低成本、微型化和无线化,未来可以广泛用于各种微小卫星。 展开更多
关键词 无线 太阳敏感器 四象限探测器 超级电容
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普鲁卡因对结肠癌细胞中孕酮及脂联素受体3甲基化的影响 被引量:1
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作者 梁田田 李日恒 +8 位作者 张爱民 郑三龙 丁丽坤 彭鑫宇 问明 郗欣 郭剑 王伟森 李杰 《中国现代医学杂志》 CAS 2020年第4期16-20,共5页
目的研究孕酮及脂联素受体3(PAQR3)在结肠癌细胞中的表达水平及甲基化状态,检测普鲁卡因逆转PAQR3甲基化的作用。方法通过RT-PCR检测PAQR3在结肠癌细胞株(SW620、COLO205及SW480)中的表达水平;甲基化特异性聚合酶链反应检测PAQR3在结直... 目的研究孕酮及脂联素受体3(PAQR3)在结肠癌细胞中的表达水平及甲基化状态,检测普鲁卡因逆转PAQR3甲基化的作用。方法通过RT-PCR检测PAQR3在结肠癌细胞株(SW620、COLO205及SW480)中的表达水平;甲基化特异性聚合酶链反应检测PAQR3在结直肠癌细胞中的甲基化状态及药物处理后的甲基化状态;Western blotting检测不同浓度普鲁卡因处理SW480细胞中PAQR3蛋白的表达。结果PAQR3在SW620结肠癌细胞中的表达量高于COLO205和SW480(P<0.05)。3种结肠癌细胞株可见PAQR3基因甲基化特异性扩增产物条带,且甲基化百分比比较,差异有统计学意义(P<0.05)。SW620细胞较SW480和COLO205细胞中PAQR3甲基化百分比高(P<0.05);普鲁卡因处理后3种结肠癌细胞株可见甲基化特异性扩增产物条带和非甲基化特异性扩增条带,且3种细胞中PAQR3甲基化百分比降低(P<0.05)。对照组与实验组PAQR3蛋白相对表达量比较,差异有统计学意义(P<0.05)。与对照组比较,实验组PAQR3蛋白相对表达量升高(P<0.05)。结论PAQR3在结直肠癌中低表达,可能与其发生启动子甲基化有关。普鲁卡因可部分逆转结直肠癌细胞中PAQR3甲基化状态,上调PAQR3蛋白表达量。 展开更多
关键词 结直肠肿瘤 甲基化 基因表达
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Effects of warming and clipping on plant and soil properties of an alpine meadow in the Qinghai-Tibetan Plateau, China 被引量:15
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作者 Man Hou XU Fei PENG +4 位作者 Quan Gang YOU jian guo Xia Fei TIAN Min LIU Xian XUE 《Journal of Arid Land》 SCIE CSCD 2015年第2期189-204,共16页
Climate warming and livestock grazing are known to have great influences on alpine ecosystems like those of the Qinghai-Tibetan Plateau (QTP) in China. However, it is lacking of studies on the effects of warming and... Climate warming and livestock grazing are known to have great influences on alpine ecosystems like those of the Qinghai-Tibetan Plateau (QTP) in China. However, it is lacking of studies on the effects of warming and grazing on plant and soil properties in these alpine ecosystems. In this study, we reported the related research from manipulative experiment in 2010-2012 in the QTP. The aim of this study was to investigate the individual and combined effects of warming and clipping on plant and soil properties in the alpine meadow ecosystem. Infrared radiators were used to simulate climate warming starting in July 2010, while clipping was performed once in Octo- ber 2011 to simulate the local livestock grazing. The experiment was designed as a randomized block consisting of five replications and four treatments: control (CK), warming (W), clipping (C) and warming+clipping combination (WC). The plant and soil properties were investigated in the growing season of the alpine meadow in 2012. The results showed that W and WC treatments significantly decreased relative humidity at 20-cm height above ground as well as significantly increases air temperature at the same height, surface temperature, and soil temperature at the depth of 0-30 cm. However, the C treatment did not significantly decrease soil moisture and soil temperature at the depth of 0-60 cm. Relative to CK, vegetation height and species number increased significantly in W and WC treatment, respectively, while vegetation aboveground biomass decreased significantly in C treatment in the early growing season. However, vegetation cover, species diversity, belowground biomass and soil properties at the depth of 0-30 cm did not differ significantly in W, C and WC treatments. Soil moisture increased at the depth of 40-100 cm in W and WC treatments, while belowground biomass, soil activated carbon, organic carbon and total nitrogen increased in the 30-50 cm soil layer in W, C and WC treatments. Although the initial responses of plant and soil properties to experimental warming and clipping were slow and weak, the drought induced by the down- ward shift of soil moisture in the upper soil layers may induce plant belowground biomass to transfer to the deeper soil layers. This movement would modify the distributions of soil activated carbon, organic carbon and total nitrogen However, long-term data collection is needed to further explain this interesting phenomenon. 展开更多
关键词 simulated warming OVERGRAZING soil property plant property alpine meadow ecosystem Qinghai-Tibetan Plateau
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Differences in brain-derived neurotrophic factor gene polymorphisms between acute ischemic stroke patients and healthy controls in the Han population of southwest China 被引量:7
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作者 Jie Zhou Meng-Meng Ma +4 位作者 Jing-Huan Fang Lei Zhao Mu-Ke Zhou jian guo Li He 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第8期1404-1411,共8页
Single-nucleotide polymorphisms in the brain-derived neurotrophic factor gene may affect the secretion and function of brain-derived neurotrophic factor, thereby affecting the occurrence, severity and prognosis of isc... Single-nucleotide polymorphisms in the brain-derived neurotrophic factor gene may affect the secretion and function of brain-derived neurotrophic factor, thereby affecting the occurrence, severity and prognosis of ischemic stroke. This case-control study included 778 patients (475 males and 303 females, mean age of 64.0 ± 12.6 years) in the acute phase of ischemic stroke and 865 control subjects (438 males and 427 females, mean age of 51.7 ± 14.7 years) from the Department of Neurology, Wes: China Hospital, Sichuan University, China between September 2011 and December 2014. The patients' severities of neurological defici:s in the acute phase were assessed using the National Institutes of Health Stroke Scale immediately after admission to hospital. The ischemic stroke patients were divided into different subtypes according to the Trial of Org 10172 in Acute Stroke Treatment classification. Early prognosis was evaluated using the Modified Rankin Scale when the patients were discharged. Genomic DNA was extracted from peripheral blood of participants. Genotyping of rs7124442 and rs6265 was performed using Kompetitive Allele Specific polymerase chain reaction genotyping technology. Our results demonstrated that patients who carried the C allele of the rs7124442 locus had a lower risk of poor prognosis than the T allele carriers (odds ratio [OR]= 0.67;95% confidence interval [CI]: 0.45-1.00;P = 0.048). The patients with the CC or TC genotype also exhibited lower risk than TT carriers (OR = 0.65;95% CI: 0.42-1.00;P = 0.049). The AA genotype at the rs6265 locus was associated with the occurrence of ischemic stroke in patients with large-artery atherosclerosis (OR = 0.5& 95% CI: 0.37-0.90;P = 0.015). We found that the C allele (CC and TC genotypes) at the rs7124442 locus may be protective for the prognosis of ischemic stroke. The AA genotype at the rs6265 locus is likely a protective factor against the occurrence of ischemic stroke in patients with large-artery atherosclerosis. The study protocol was approved by the Ethics Committee of West China Hospital of Sichuan University, China (approval ID number 2008,4]) on July 25, 2008. 展开更多
关键词 nerve REGENERATION ischemic STROKE BRAIN-DERIVED NEUROTROPHIC factor single-nucleotide polymorphism risk STROKE severity prognosis rs6265 rs7124442 neural REGENERATION
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Thermodynamic modeling and phase diagram prediction of salt lake brine systems.Ⅰ. Aqueous Mg^2+–Ca^2+–Cl^- binary and ternary systems 被引量:10
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作者 Huan Zhou Xiaolong Gu +4 位作者 Yaping Dai Jingjing Tang jian guo Guangbi Li Xiaoqin Bai 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2020年第9期2391-2408,共18页
Salt lake brine is a complex salt-water system under natural environment.Although many models can express the thermodynamic properties and phase equilibrium of electrolyte aqueous solution,the multi-temperature charac... Salt lake brine is a complex salt-water system under natural environment.Although many models can express the thermodynamic properties and phase equilibrium of electrolyte aqueous solution,the multi-temperature characteristics and predictability are still the goals of model development.In this study,a comprehensive thermodynamic model system is re-established based on the eNRTL model and some improvements:(1) new expression of long-range electrostatic term with symmetrical reference state is proposed to handle the electrolyte solution covering entire concentration range;(2) the temperature dependence of the binary interaction parameters is formulated with a Gibbs Helmholtz expression containing three temperature coefficients,the liquid parameters,which associated with Gibbs energy,enthalpy,and heat capacity contribution;and(3) liquid parameters and solid species data are regressed from properties and solubility data at full temperature range.Together the activity coefficient model,property models and parameters of liquid and solid offer a comprehensive thermodynamic model system for the typical bittern of MgCl2-CaCl2-H2 O binary and ternary systems,and it shows excellent agreement with the literature data for the ternary and binary systems.The successful prediction of complete phase diagram of ternary system shows that the model has the ability to deal with high concentration and high non-idealitv system,and the ability to extrapolate the temperature. 展开更多
关键词 Aqueous electrolytes Comprehensive thermodynamic model MgCl2-CaCl2-H2O Phase diagram Thermodynamic properties
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Risk Factors for Strangulated Ovarian Hernia in Female Infants:the Role of Ovarian Volume 被引量:6
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作者 Yu CHEN Xiang-zhi PENG +6 位作者 Wei LU Kai ZHENG jian guo Hua NIE Xiao-jie SONG Yan ZHANG Jing YANG 《Current Medical Science》 SCIE CAS 2018年第6期1032-1037,共6页
The risk factors associated with strangulated ovarian hernia (SOH)in female patients (<1 year old)were identified.A retrospective analysis was conducted regarding the data from 2006 to 2017.The patients were divide... The risk factors associated with strangulated ovarian hernia (SOH)in female patients (<1 year old)were identified.A retrospective analysis was conducted regarding the data from 2006 to 2017.The patients were divided into 2groups:SOH group (n=9)and non-SOH group (n=23). Patient demographics,clinical signs,preoperative examinations and intraoperative findings were compared between the two groups,and risk factors for SOH were tested using a binary logistic regression model.To explore whether greater ovary was more likely to be twisted,leading to SOH, all the patients were divided into ovary volume <5cm^3 and ≥5cm^3 groups and the association between ovarian volume and ovary torsion was assessed.Among a total of 32 female patients (<1year old)with incarcerated ovarian herniation,9 patients developed SOH.The single variate analysis revealed that times of manual reduction,ovarian volume,ovary with or without multiple cysts,ovary torsion or not and angle of ovary torsion were found to be significant factors associated with SOH.The multivariate analysis showed ovarian volume was evidenced as an independent risk factor for SOH.Furthermore,the incidence of ovary torsion was significantly higher in ovarian volume ≥5cm^3 group than in ovarian volume <5cm^3 group,indicating that larger ovary was more likely to result in ovary torsion,leading to SOH.Our study demonstrated that the odds of SOH increased with increasing ovarian volume in female patients (<1 year old)because the relatively greater ovary at this age was more likely to be incarcerated and twisted,leading to SOH. 展开更多
关键词 INGUINAL HERNIA INCARCERATION OVARY STRANGULATION risk factors PEDIATRICS
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小而密低密度脂蛋白、游离脂肪酸水平与2型糖尿病患者合并动脉粥样硬化的相关性研究 被引量:15
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作者 吴义忠 蹇国 张婷 《检验医学与临床》 CAS 2020年第14期2007-2010,共4页
目的探讨血清小而密低密度脂蛋白(sd-LDL)、游离脂肪酸(FFA)水平与2型糖尿病(T2DM)患者合并动脉粥样硬化的相关性。方法选取广元市中心医院已确诊T2DM的患者80例作为T2DM组,以及同期40例健康体检者作为对照组。采用雅培c16000全自动生... 目的探讨血清小而密低密度脂蛋白(sd-LDL)、游离脂肪酸(FFA)水平与2型糖尿病(T2DM)患者合并动脉粥样硬化的相关性。方法选取广元市中心医院已确诊T2DM的患者80例作为T2DM组,以及同期40例健康体检者作为对照组。采用雅培c16000全自动生化分析仪测定高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、sd-LDL、FFA和空腹血糖(FPG)的水平,采用高效液相色谱法测定糖化血红蛋白(HbA1c)水平。采用飞利浦HD15型彩色超声仪检测颈总动脉内膜-中层厚度(IMT),并据此将患者分为IMT正常组和IMT增厚组。结果T2DM组LDL-C、sd-LDL、FFA、FPG和HbA1c水平均高于对照组,差异有统计学意义(P<0.05)。T2DM组HDL-C水平低于对照组,差异有统计学意义(P<0.05)。IMT增厚组与IMT正常组比较,LDL-C、sd-LDL、FFA和HbA1c水平差异均有统计学意义(P<0.05),HDL-C、FPG水平差异无统计学意义(P>0.05)。IMT增厚组sd-LDL水平与LDL-C水平呈极强相关(r=0.826,P<0.05),与FFA水平呈弱相关(r=0.378,P<0.05);FFA水平与HDL-C水平呈中等相关(r=-0.478,P<0.05)。结论血清LDL-C、sd-LDL、FFA、FPG和HbA1c水平与T2DM的发生和发展密切相关;T2DM患者血清LDL-C、sd-LDL、FFA和HbA1c水平与其动脉粥样硬化的发生有一定的关系;同时检测sd-LDL、LDL-C和FFA水平,对于预测T2DM并发动脉粥样硬化具有一定的临床价值。 展开更多
关键词 2型糖尿病 动脉粥样硬化 小而密低密度脂蛋白 游离脂肪酸
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血清学指标联合检测在类风湿关节炎诊断中的应用 被引量:4
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作者 吴义忠 李海军 +1 位作者 蹇国 罗清松 《实用检验医师杂志》 2021年第1期49-52,共4页
目的探讨血清学指标抗环瓜氨酸肽抗体(Anti-CCP)、血清淀粉样蛋白A(SAA)、类风湿因子(RF)和C-反应蛋白(CRP)联合检测在类风湿关节炎(RA)诊断中的应用。方法选择2018年1月—2019年6月广元市中心医院收治的136例确诊RA患者作为RA组,另选... 目的探讨血清学指标抗环瓜氨酸肽抗体(Anti-CCP)、血清淀粉样蛋白A(SAA)、类风湿因子(RF)和C-反应蛋白(CRP)联合检测在类风湿关节炎(RA)诊断中的应用。方法选择2018年1月—2019年6月广元市中心医院收治的136例确诊RA患者作为RA组,另选择同期30例确诊为其他自身免疫性疾病患者作为非RA组,以同期30名健康体检者作为正常对照组。收集所有受检对象的血清样本,采用免疫比浊法测定SAA、RF、CRP水平,采用化学发光微粒子免疫检测法测定Anti-CCP水平;比较不同指标单独检测与联合检测对RA的诊断效能,采用Pearson相关性分析进行不同指标间的相关性分析。结果RA组Anti-CCP、SAA、RF、CRP水平均明显高于非RA组和正常对照组〔Anti-CCP(kU/L):55.40(3.90,183.15)比0.75(0.50,1.60)、0.50(0.50,1.25),SAA(mg/L):18.00(4.60,122.93)比9.40(4.73,15.73)、2.00(0.55,2.80),RF(kU/L):82.50(31.75,283.75)比17.50(14.25,25.25)、13.00(8.00,14.00),CRP(mg/L):7.25(6.48,37.98)比5.45(4.38,12.60)、0.70(0.30,1.28)〕,差异均有统计学意义(与非RA组比较,均P<0.05;与正常对照组比较,均P<0.01)。非RA组SAA、RF和CRP水平均明显高于正常对照组(均P<0.05),Anti-CCP水平与正常对照组比较差异无统计学意义(P>0.05)。4个指标联合检测RA的敏感度、准确度和阴性预测值均明显高于Anti-CCP、SAA、RF、CRP单独检测时(敏感度:97.8%比72.8%、49.3%、86.0%、56.6%,准确度:94.9%比79.1%、56.6%、84.2%、61.2%,阴性预测值:94.6%比60.2%、38.9%、71.6%、42.2%,均P<0.05);Anti-CCP单独检测的特异度和阳性预测值均明显高于SAA、RF、CRP单独检测(特异度:93.3%比73.3%、80.0%、71.7%,阳性预测值:96.1%比80.7%、90.7%、81.9%,均P<0.05)。RA组患者血清SAA与CRP水平呈中等相关性(y=1.365x+37.361,r=0.545,P=0.000),其余指标间均无相关性。结论联合检测血清Anti-CCP、SAA、RF、CRP来诊断RA可以减少误诊和漏诊,具有十分重要的临床应用价值。 展开更多
关键词 类风湿关节炎 抗环瓜氨酸肽抗体 血清淀粉样蛋白A 类风湿因子 C-反应蛋白
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Aquaporin-1 down regulation associated with inhibiting cell viability and inducing apoptosis of human lens epithelial cells 被引量:5
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作者 Hong-Hua Zheng guo-Xing Xu +2 位作者 jian guo Li-Cheng Fu Yao Yao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第1期15-20,共6页
AIM:To investigate the role of Aquaporin-1(AQP-1)in lens epithelial cells(LECs)and its potential target genes.AQP-1 is specifically expressed in LECs of eyes and is significant for lens homeostasis and transparen... AIM:To investigate the role of Aquaporin-1(AQP-1)in lens epithelial cells(LECs)and its potential target genes.AQP-1 is specifically expressed in LECs of eyes and is significant for lens homeostasis and transparency maintenance.Herein,AQP-1 expression in LECs was investigated to evaluate its influence on cell survival in association with its potential role in cataract formation.·M ETHODS:LECs were transfected with lentivirus carrying AQP-1 small interfering RNA(si RNA).Real-time polymerase chain reaction(PCR)and Western blotting were conducted to detect AQP-1 expression in LECs from different groups.Meanwhile,cell counting kit-8(CCK-8)assay and flow cytometry were performed to measure LEC proliferation and apoptosis,respectively.·RESULTS:AQP-1 expression was significantly reduced in LECs,both at m RNA and protein levels(〈0.05),after si RNA treatment.Decreased cell viability was detected by CCK-8 assay in LECs with si RNA interference,compared to control cells(〈0.05).The apoptosis rate significantly increased in cells after si RNA interference(〈0.05).·CONCLUSION:The decreased cell viability following AQP-1 down regulation is largely due to its induction of apoptosis of LECs.AQP-1 reduction might lead to changes of physiological functions in LECs,which might be associated with the occurrence and development of cataracts. 展开更多
关键词 AQUAPORIN-1 small interfering RNA lensepithelial cells proliferation APOPTOSIS cell counting kit-8 flow cytometry
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Effects and mechanism of adenovirus-mediated phosphatase and tension homologue deleted on chromosome ten gene on collagen deposition in rat liver fibrosis 被引量:4
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作者 Shu-rui Xie Jun-yan An +4 位作者 Li-bo Zheng Xiao-Xia Huo jian guo David Shih Xiao-Lan Zhang 《World Journal of Gastroenterology》 SCIE CAS 2017年第32期5904-5912,共9页
AIM To evaluate the effects of phosphatase and tension homologue deleted on chromosome ten(PTEN) gene on collagen metabolism in hepatic fibrosis and the underlying mechanisms.METHODS rat primary hepatic stellate cells... AIM To evaluate the effects of phosphatase and tension homologue deleted on chromosome ten(PTEN) gene on collagen metabolism in hepatic fibrosis and the underlying mechanisms.METHODS rat primary hepatic stellate cells(HSCs) and human LX-2 cells were transfected with adenovirus containing c DNA constructs encoding wild-type PTEN(Ad-PTEN), PTEN mutant G129 E gene(Ad-G129E), and r NA interference constructs targeting the PTEN sequence PTEN short hairpin r NA to up-regulate and downregulate the expression of PTEN. HSCs were assayed using fluorescent microscopy, real-time polymerase chain reaction, and western blotting. Moreover, a CCl_4-induced rat hepatic fibrosis model was established to investigate the in vivo effects. Hematoxylin and eosin, and Masson's trichrome were used to assess the histological changes. The expression of collagen Ⅰ and Ⅲ was assessed using immunohistochemistry and western blot analysis.RESULTS Elevated expression of PTEN gene reduced serum levels of alanine transaminase and aspartate transaminase, decreased collagen deposition in the liver, and reduced hepatocyte necrosis. In contrast, knockdown of PTEN expression had an opposite effect, such as increased collagen deposition in the liver, and was molecularly characterized by the increased expression of matrix metalloproteinase(MMP)-13(P < 0.01) and MMP-2(P < 0.01), as well as decreased expression of the tissue inhibitor of metalloproteinase(TIMP)-1(P < 0.01) and TIMP-2(P < 0.01).CONCLUSION These data indicated that gene therapy using recombinant adenovirus encoding PTEN might be a novel way of treating hepatic fibrosis. 展开更多
关键词 Collagen metabolism Hepatic stellate cells Phosphatase and tension homologue deleted on chromosome ten PTEN Gene therapy Hepatic fibrosis
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Integrated system of comprehensive utilizing the concentrated brine of Yuncheng salt-lake basing on salt-forming diagram 被引量:3
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作者 Huan Zhou Jingjing Tang +3 位作者 jian guo Yaping Dai Guangbi Li Bo Yan 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2019年第1期182-190,共9页
The comprehensive utilization and environment-friendliness of processes for recovering fresh water or valuable salt from seawater, salt-lakes, or mineral deposits are of utmost importance for sustainable development.O... The comprehensive utilization and environment-friendliness of processes for recovering fresh water or valuable salt from seawater, salt-lakes, or mineral deposits are of utmost importance for sustainable development.One primitive sustainable process for recovering salt from sodium-sulfate-type brine in Yuncheng salt lake had been considered one of the greatest inventions of ancient China, however, the replaced process of mass extraction of single Na_2SO_4 in recent years, has reduced a large amount of residual brine.In this research, relying on the salt-forming diagram in the non-equilibrium state, the technical secrets of ancient salt processes were uncovered, and a new comprehensive utilization system was proposed and tested experimentally.The new system includes a vacuum salt-making process and a normal pressure kieserite process, which can gradually eliminate the existed waste liquid and aid in the sustainable development of the Yuncheng salt-lake.The continuous experiment of salt-making process running stably in the double salt region without double salt formation, which proves the feasibility of salt-forming diagram applied in industrial process.Thus salt-forming diagram would be extremely valuable to industry process design and control, especially, the treatment of concentrated brine. 展开更多
关键词 Phase DIAGRAM BRINE Salt-forming NONEQUILIBRIUM state COMPREHENSIVE utilization
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Reentry Attitude Tracking Control for Hypersonic Vehicle with Reaction Control Systems via Improved Model Predictive Control Approach 被引量:4
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作者 Kai Liu Zheng Hou +2 位作者 Zhiyong She jian guo 《Computer Modeling in Engineering & Sciences》 SCIE EI 2020年第1期131-148,共18页
This paper studies the reentry attitude tracking control problem for hypersonic vehicles(HSV)equipped with reaction control systems(RCS)and aerodynamic surfaces.The attitude dynamical model of the hypersonic vehicles ... This paper studies the reentry attitude tracking control problem for hypersonic vehicles(HSV)equipped with reaction control systems(RCS)and aerodynamic surfaces.The attitude dynamical model of the hypersonic vehicles is established,and the simplified longitudinal and lateral dynamic models are obtained,respectively.Then,the compound control allocation strategy is provided and the model predictive controller is designed for the pitch channel.Furthermore,considering the complicated jet interaction effect of HSV during RCS is working,an improved model predictive control approach is presented by introducing the online parameter estimation of the jet interaction coefficient for dealing with the uncertainty and disturbance.Moreover,considering the strong coupling effect between the yaw channel and roll channel,a coupled model predictive controller is designed by introducing the feedback of sideslip angle into the roll control channel to eliminate the coupling effect.Finally,the comparison simulations using the classical control method,MPC and IMPC approach are given to demonstrate the effectiveness and efficiency of the presented IMPC scheme. 展开更多
关键词 Hypersonic vehicle reentry attitude control mode predictive control jet interference factor reaction control systems
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Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis 被引量:3
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作者 jian guo Mu Wang +1 位作者 Jun-Ping Wang Chang-Xin Wu 《World Journal of Gastroenterology》 SCIE CAS 2019年第43期6386-6403,共18页
BACKGROUND Hepatocellular carcinoma(HCC)is now the most common primary liver malignancy worldwide,and multiple risk factors attribute to the occurrence and development of HCC.Recently,increasing studies suggest that u... BACKGROUND Hepatocellular carcinoma(HCC)is now the most common primary liver malignancy worldwide,and multiple risk factors attribute to the occurrence and development of HCC.Recently,increasing studies suggest that ubiquitinconjugating enzyme E2T(UBE2T)serves as a promising prognostic factor in human cancers,although the molecular mechanism of UBE2T in HCC remains unclear.AIM To investigate the clinical relevance and role of UBE2T in HCC development.METHODS UBE2T expression in HCC tissues from the TCGA database and its association with patient survival were analyzed.A lentivirus-mediated strategy was used to knock down UBE2T in HCC cells.qRT-PCR and Western blot assays were performed to check the effect of UBE2T silencing in HCC cells.Cell growth in vitro and in vivo was analyzed by multiparametric high-content screening and the xenograft tumorigenicity assay,respectively.Cell cycle distribution and apoptosis were determined by flow cytometry.The genes regulated by UBE2T were profiled by microarray assay.RESULTS UBE2T was overexpressed in HCC tissues compared with paired and non-paired normal tissues.High expression of UBE2T predicted a poor overall survival in HCC patients.In vitro,lentivirus-mediated UBE2T knockdown significantly reduced the viability of both SMMC-7721 and BEL-7404 cells.In vivo,the xenograft tumorigenesis of SMMC-7721 cells was largely attenuated by UBE2T silencing.The cell cycle was arrested at G1/S phase in SMMC-7721 and BEL-7404 cells with UBE2T knockdown.Furthermore,apoptosis was increased by UBE2T knockdown.At the molecular level,numerous genes were dysregulated after UBE2T silencing,including IL-1B,FOSL1,PTGS2,and BMP6.CONCLUSION UBE2T plays an important role in cell cycle progression,apoptosis,and HCC development. 展开更多
关键词 HEPATOCELLULAR carcinoma Ubiquitin-conjugating enzyme E2T Cell cycle APOPTOSIS TUMORIGENESIS
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Mechanical Properties of Lime-Fly Ash-Sulphate Aluminum Cement Stabilized Loess 被引量:5
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作者 Liang Jia Chunxiang Li jian guo 《Journal of Renewable Materials》 SCIE EI 2020年第10期1357-1373,共17页
Lime-fly ash stabilized loess has a poor early strength,which results in a later traffic opening time when it is used as road-base materials.Consideration of the significant early strength characteristics of sulphate ... Lime-fly ash stabilized loess has a poor early strength,which results in a later traffic opening time when it is used as road-base materials.Consideration of the significant early strength characteristics of sulphate aluminum cement(SAC),it is always added into the lime-fly ash mixtures to improve the early strength of stabilized loess.However,there is a scarcity of research on the mechanical behavior of lime-fly ash-SAC stabilized loess and there is a lack of quantitative evaluation of loess stabilized with binder materials.This research explored the effects of the amount of binder materials,curing time and porosity on the unconfined compressive strength(UCS),splitting tensile strength(STS),cohesion(c)and friction angle(φ)of lime-fly ash-SAC stabilized loess by a series of unconfined compressive tests(UCT)and splitting tensile tests(STT).The results indicate that an increase in curing time and a decrease in porosity lead to a continuous increase in the UCS and STS for lime-fly ash-SAC stabilized loess.The addition of SAC has a prominent enhancement in the early strength of lime-fly ash-SAC stabilized loess.When the curing time,porosity,and binder content were constant,the UCS and STS increase with increasing SAC content;For a stabilized loess with 30%binder content and 5%SAC content after 1 day of curing,the UCS was greater than 0.7 MPa,which meets the requirement of opening traffic,so lime-fly ash-SAC stabilized loess could be used as an excellent maintenance material for road-base;In accordance with the analysis of testing data,empirical relationships between the UCS and STS of lime-fly-SAC stabilized loess and key effect factors(binder materials content,curing time and porosity)were developed,which can provide references for reasonably selecting the amount of binder materials,compaction degree and curing period to meet the required strength of practical engineering.Finally,based on the Mohr-Coulomb theory and the above empirical relationships,a simpler method for calculating the c andφof stabilized loess was proposed,with which,the shear strength parameters can be determined only by UCT or STT. 展开更多
关键词 LOESS sulphate aluminum cement unconfined compressive strength splitting tensile strength
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Expression of nerve growth factor precursor, mature nerve growth factor and their receptors during cerebral ischemia-reperfusion injury 被引量:3
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作者 guoqian He jian guo +4 位作者 Jiachuan Duan Wenming Xu Ning Chen Hongxia Li Li He 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第22期1701-1708,共8页
We investigated nerve growth factor precursor (proNGF) and mature NGF expression in ischemic and non-ischemic cortices after cerebral ischemia-reperfusion injury. In both ischemic and non-ischemic cortices, proNGF w... We investigated nerve growth factor precursor (proNGF) and mature NGF expression in ischemic and non-ischemic cortices after cerebral ischemia-reperfusion injury. In both ischemic and non-ischemic cortices, proNGF was found to be present in the extracellular space and cytoplasm. In addition, mature NGF was expressed in extracellular space, but with a very low signal. In ischemic cortex only, proNGF was significantly decreased, reaching a minimal level at 1 day. Mature NGF was increased at 4 hours, then reached a minimal level at 3 days. The p75 neurotrophin receptor (p75NTR) was significantly decreased after ischemia, and increased at 3 days after ischemia. These results confirmed that proNGF was the predominant form of NGF during the pathological process of cerebral ischemia-repeffusion injury. In addition, our findings suggest that ischemic injury may influence the conversion of proNGF to mature NGF, and that proNGF/p75NTR may be involved in reperfusion injury. 展开更多
关键词 cerebral ischemia-reperfusion injury nerve growth factor precursor mature nerve growth factor p75 neurotrophin receptor cell apoptosis
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Experimental investigation of erosion rate for gas-solid two-phase flow in 304 stainless/L245 carbon steel 被引量:3
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作者 Bingyuan Hong Yanbo Li +6 位作者 Xiaoping Li Gen Li ong Huang Shuaipeng Ji Weidong Li Jing Gong jian guo 《Petroleum Science》 SCIE CAS CSCD 2022年第3期1347-1360,共14页
Erosion is one of the most concerning issues in pipeline flow assurance for the Oil&Gas pipeline industries,which can easily lead to wall thinning,perforation leakage,and other crucial safety risks to the steady o... Erosion is one of the most concerning issues in pipeline flow assurance for the Oil&Gas pipeline industries,which can easily lead to wall thinning,perforation leakage,and other crucial safety risks to the steady operation of pipelines.In this research,a novel experimental device is designed to investigate the erosion characteristics of 304 stainless and L245 carbon steel in the gas-solid two-phase flow.Regarding the impacts on erosion rate,the typical factors such as gas velocity,impact angle,erosion time,particle material and target material are individually observed and comprehensive analyzed with the assistance of apparent morphology characterized via Scanning Electron Microscope.Experimental results show that the severest erosion occurs when the angle reaches approximate 30°whether eroded by type I or type II particles,which is observed in both two types of steel.Concretely,304 stainless steel and L245 carbon steel appear to be cut at low angles,and impacted at high angles to form erosion pits.In the steady operational state,the erosion rate is insensitive to the short erosion time and free from the influences caused by the“erosion latent period”.Based on the comparison between experimental data and numerical results generated by existing erosion models,a modified model with low tolerance(<3%),high feasibility and strong consistency is proposed to make an accurate prediction of the erosion in terms of two types of steel under various industrial conditions. 展开更多
关键词 Gas-solid flow EROSION 304 stainless L245 carbon steel Erosion model
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