BACKGROUND Myocardial ischemia-reperfusion(I/R) is a serious and irreversible injury. Bone marrow-derived mesenchymal stem cells(MSCs) is considered to be a potential therapy for I/R injury due to the paracrine effect...BACKGROUND Myocardial ischemia-reperfusion(I/R) is a serious and irreversible injury. Bone marrow-derived mesenchymal stem cells(MSCs) is considered to be a potential therapy for I/R injury due to the paracrine effects. High-mobility group box1(HMGB1) is a novel mediator in MSC and regulates the response of inflammation injury. Signal Transduction and Transcription Activator 3(STAT3) is a critical transcription factor and important for release of paracrine factors. However, the relationship between HMGB1 and STAT3 in paracrine effect of MSC remains unknown.METHODS In vitro, hypoxia/reoxygenation injury model was established by Anaero Pack System and examined by Annexin V flow cytometry, CCK8 assay and morphology observation. Detection of apoptotic proteins and protein expression of HMGB1and STAT3 by Western blot.RESULTS The conditioned medium of MSCs with or without LPS pretreatment was cocultured with H9C2 cells for 24 h before hypoxia treatment and MSC showed obvious cardiomyocytes protect role, as evidence by decreased apoptosis rate and improved cells viability, and LPS pretreated MSC exhibited better protect role than untreated MSC. However, such effect was abolished in HMGB1 deficiency group, silencing HMGB1 decreased the secretion of vascular endothelial growth factor(VEGF), hepatocyte growth factor(HGF), insulin growth factor(IGF), cell viability, and the expression of STAT3. Furthermore, STAT3 silence attenuated the protective effect of LPS in MSC.CONCLUSIONS These findings suggested that LPS improved MSC-mediated cardiomyocytes protection by HMGB1/STAT3signaling.展开更多
OOV term translation plays an important role in natural language processing. Although many researchers in the past have endeavored to solve the OOV term translation problems, but none existing methods offer definition...OOV term translation plays an important role in natural language processing. Although many researchers in the past have endeavored to solve the OOV term translation problems, but none existing methods offer definition or context information of OOV terms. Furthermore, non-existing methods focus on cross-language definition retrieval for OOV terms. Never the less, it has always been so difficult to evaluate the correctness of an OOV term translation without domain specific knowledge and correct references. Our English definition ranking method differentiate the types of OOV terms, and applies different methods for translation extraction. Our English definition ranking method also extracts multilingual context information and monolingual definitions of OOV terms. In addition, we propose a novel cross-language definition retrieval system for OOV terms. Never the less, we propose an auto re-evaluation method to evaluate the correctness of OOV translations and definitions. Our methods achieve high performances against existing methods.展开更多
The optical properties of coatings pigmented with different black colorants were systematically investigated and their surface temperatures and cooling energy savings were estimated. The black coatings pigmented with ...The optical properties of coatings pigmented with different black colorants were systematically investigated and their surface temperatures and cooling energy savings were estimated. The black coatings pigmented with chromite iron nickel black and manganese ferrite black spinel colorants are not cool enough to be energy efficient cool black coatings. The cool black coatings pigmented with NIR-transmitting perylene black and dioxazine purple colorants possess a green shade and a violet shade, respectively. The estimated surface temperature reduction values and annual cooling energy savings in Beijing range from 3.0°C and 1.21 kWhm-2yr-1 for the black coating pigmented with chromite iron nickel colorant to 13.8°C and 5.52 kWhm-2yr-1 for the black coating pigmented with dioxazine purple colorant, respectively.展开更多
To the Editor:Primary familial brain calcification (PFBC) is a rare genetically degenerative disease that is generally characterized by symmetrical,bilateral calcinosis in the basal ganglia,thalamus,dentate nuclei,and...To the Editor:Primary familial brain calcification (PFBC) is a rare genetically degenerative disease that is generally characterized by symmetrical,bilateral calcinosis in the basal ganglia,thalamus,dentate nuclei,and other brain regions and mainly manifests with neurological and psychiatric symptoms. A growing number of studies have reported associations between this condition and pathogenic mutations,such as those in the SLC2OA2,PDGFRB,PDGFB,XPR1,and MYORG genes, Few studies have evaluated familial clinical characteristics, such as the volume of basal ganglia calcification (VBGC), and the results of clinical tests to determine serum levels of calcium (Ca),magnesium (Mg),phosphorus (P),iron (Fe), aluminum (A1),arsenic (As),cobalt (Co),parathyroid hormone (PTH),and calcitonin (Ct),or the relationship between patient age and the VBGC in PFBC.展开更多
基金supported by grants from the Natural Science Foundation of Liaoning Province(No.2023-MS-268)Educational Committee Foundation of Liaoning Province(LJKZ0868)+1 种基金Funds for young and middle-aged disciplinary backbones of the Second Hospital of Dalian Medical University(No.dy2yxkgg 202002)partially supported by United Fund of the Second Hospital of Dalian Medical University and Dalian Institute of Chemical physics Chinese Academy of Sciences(No.UF-QN-202005).
文摘BACKGROUND Myocardial ischemia-reperfusion(I/R) is a serious and irreversible injury. Bone marrow-derived mesenchymal stem cells(MSCs) is considered to be a potential therapy for I/R injury due to the paracrine effects. High-mobility group box1(HMGB1) is a novel mediator in MSC and regulates the response of inflammation injury. Signal Transduction and Transcription Activator 3(STAT3) is a critical transcription factor and important for release of paracrine factors. However, the relationship between HMGB1 and STAT3 in paracrine effect of MSC remains unknown.METHODS In vitro, hypoxia/reoxygenation injury model was established by Anaero Pack System and examined by Annexin V flow cytometry, CCK8 assay and morphology observation. Detection of apoptotic proteins and protein expression of HMGB1and STAT3 by Western blot.RESULTS The conditioned medium of MSCs with or without LPS pretreatment was cocultured with H9C2 cells for 24 h before hypoxia treatment and MSC showed obvious cardiomyocytes protect role, as evidence by decreased apoptosis rate and improved cells viability, and LPS pretreated MSC exhibited better protect role than untreated MSC. However, such effect was abolished in HMGB1 deficiency group, silencing HMGB1 decreased the secretion of vascular endothelial growth factor(VEGF), hepatocyte growth factor(HGF), insulin growth factor(IGF), cell viability, and the expression of STAT3. Furthermore, STAT3 silence attenuated the protective effect of LPS in MSC.CONCLUSIONS These findings suggested that LPS improved MSC-mediated cardiomyocytes protection by HMGB1/STAT3signaling.
文摘OOV term translation plays an important role in natural language processing. Although many researchers in the past have endeavored to solve the OOV term translation problems, but none existing methods offer definition or context information of OOV terms. Furthermore, non-existing methods focus on cross-language definition retrieval for OOV terms. Never the less, it has always been so difficult to evaluate the correctness of an OOV term translation without domain specific knowledge and correct references. Our English definition ranking method differentiate the types of OOV terms, and applies different methods for translation extraction. Our English definition ranking method also extracts multilingual context information and monolingual definitions of OOV terms. In addition, we propose a novel cross-language definition retrieval system for OOV terms. Never the less, we propose an auto re-evaluation method to evaluate the correctness of OOV translations and definitions. Our methods achieve high performances against existing methods.
文摘The optical properties of coatings pigmented with different black colorants were systematically investigated and their surface temperatures and cooling energy savings were estimated. The black coatings pigmented with chromite iron nickel black and manganese ferrite black spinel colorants are not cool enough to be energy efficient cool black coatings. The cool black coatings pigmented with NIR-transmitting perylene black and dioxazine purple colorants possess a green shade and a violet shade, respectively. The estimated surface temperature reduction values and annual cooling energy savings in Beijing range from 3.0°C and 1.21 kWhm-2yr-1 for the black coating pigmented with chromite iron nickel colorant to 13.8°C and 5.52 kWhm-2yr-1 for the black coating pigmented with dioxazine purple colorant, respectively.
基金the grants from the Health and Family Planning Commission of Hunan Province (No.B2015-106)the Natural Science Foundation of Hunan Province of China (No.2018JJ6012).
文摘To the Editor:Primary familial brain calcification (PFBC) is a rare genetically degenerative disease that is generally characterized by symmetrical,bilateral calcinosis in the basal ganglia,thalamus,dentate nuclei,and other brain regions and mainly manifests with neurological and psychiatric symptoms. A growing number of studies have reported associations between this condition and pathogenic mutations,such as those in the SLC2OA2,PDGFRB,PDGFB,XPR1,and MYORG genes, Few studies have evaluated familial clinical characteristics, such as the volume of basal ganglia calcification (VBGC), and the results of clinical tests to determine serum levels of calcium (Ca),magnesium (Mg),phosphorus (P),iron (Fe), aluminum (A1),arsenic (As),cobalt (Co),parathyroid hormone (PTH),and calcitonin (Ct),or the relationship between patient age and the VBGC in PFBC.
