Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle weakness.It is one of the most common genetic causes of mortality among infants aged less than 2 years.Biomar...Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle weakness.It is one of the most common genetic causes of mortality among infants aged less than 2 years.Biomarker research is currently receiving more attention,and new candidate biomarkers are constantly being discovered.This review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and cons.We also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific biomarkers.This review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future research.Furthermore,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.展开更多
Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease progression.The proteins,DNA,mRNA,and lipids carried by exosomes reflect...Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease progression.The proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of conditions.In this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s disease.Using a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western blot.The results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease severity.Furthermore,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control cells.Additionally,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated cells.Taken together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced neurotoxicity,thus providing a new therapeutic target for Parkinson’s disease.展开更多
BACKGROUND Flat bone metastases are common in patients with advanced cancers,often resulting in severe pain,limited mobility,and reduced quality of life(QOL).Traditional treatment options,such as radiotherapy or syste...BACKGROUND Flat bone metastases are common in patients with advanced cancers,often resulting in severe pain,limited mobility,and reduced quality of life(QOL).Traditional treatment options,such as radiotherapy or systemic therapies,often fail to provide sufficient pain relief or improve functional outcomes in these patients.Microwave ablation(MWA)offers advantages,such as shorter pro-cedure times and larger ablation zones,while percutaneous osteoplasty(PO)enhances bone stability and prevents pathological fractures.Despite these be-nefits,the combination of these techniques for treating flat bone metastases re-mains underexplored.AIM To evaluate the efficacy and safety of C-arm computed tomography(CT)-guided MWA combined with PO for managing painful flat bone metastases,focusing on pain relief,functional improvement,and QOL enhancement.METHODS A total of 45 patients with refractory moderate-to-severe pain resulting from flat bone metastases who underwent C-arm CT-guided MWA combined with PO between January 2015 and January 2021 were included.The efficacy of the pro-cedure was assessed by changes in the visual analog scale(VAS),Oswestry disability index(ODI),and QOL,as well as the occurrence of complications.Tumor response was evaluated using RECIST v1.1 and mRECIST criteria,with overall response rate(ORR)and disease control rate(DCR)as the primary end-points.RESULTS No serious complications were observed in any of the patients.A significant reduction in VAS and ODI was noted at 1 week,1 month,and 3 months post-procedure.A marked improvement in QOL was observed at all follow-up points.Bone cement extravasation was observed in 10 patients;however,none exhibited significant clinical symptoms.Based on RECIST v1.1,the ORR was 26.7%and the DCR was 88.9%.The mRECIST evaluation revealed a higher ORR of 51.1%and DCR of 88.9%.CONCLUSION C-arm CT-guided MWA with PO provides a dependable and effective strategy for managing flat bone metastases.It demonstrates significant pain relief,improved functional outcomes,and enhanced QOL.This treatment combination also shows promising tumor response rates with a low complication profile.展开更多
We are now in the era of big data for biodiversity science.More and larger datasets on species geographic distributions,community composition,and functional traits are now becoming more increasingly than ever before.C...We are now in the era of big data for biodiversity science.More and larger datasets on species geographic distributions,community composition,and functional traits are now becoming more increasingly than ever before.Correctly applying taxon names is a prerequisite for robust biodiversity studies of all taxonomic groups.展开更多
The incidence of in-hospital cardiac arrest (IHCA) has increased over the past decade,with more than half occurring in intensive care units (ICUs).^([1])ICU cardiac arrest (ICU-CA)presents unique challenges,with worse...The incidence of in-hospital cardiac arrest (IHCA) has increased over the past decade,with more than half occurring in intensive care units (ICUs).^([1])ICU cardiac arrest (ICU-CA)presents unique challenges,with worse outcomes than those in monitored wards,highlighting the need for early detection and intervention.^([2])Up to 80%of patients exhibit signs of deterioration hours before IHCA.^([3])Although early warning scores based on vital signs are useful,their eff ectiveness in ICUs is limited due to abnormal physiological parameters.^([4])Laboratory markers,such as sodium,potassium,and lactate,are predictive of poor outcomes,^([5])but static measurements may not capture the patient’s trajectory.Trends in laboratory indicators,such as variability and extremes,may offer better predictive value.^([6])This study aimed to evaluate ICU-CA predictive factors,with a focus on vital signs and trends of laboratory indicators.展开更多
Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and t...Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).展开更多
A comparative comparative study on the detrital mineral composition of stream sediments of the Yangtze River (Changjiang) and Yellow River (Huanghe) shows that, light minerals of the Yangtze River basin were mainly qu...A comparative comparative study on the detrital mineral composition of stream sediments of the Yangtze River (Changjiang) and Yellow River (Huanghe) shows that, light minerals of the Yangtze River basin were mainly quartz, feldspar, and detritus, the compositional characteristics of light minerals differed among tributaries, the main stream had a generally higher maturity index than tributaries;heavy mineral content tended to decrease progressively from the upper stream to lower stream of the Yangtze River, the primary assemblage was magnetite-hornblende-augite-garnet-epidote, and diagnostic minerals of different river basins were capable of indicating the nature and distribution of the source rock. Detrital mineral assemblages in sediments of tributaries and the main stream of the Yellow River were basically similar, Primary heavy mineral assemblage was opaque mineral-garnet-epidote-carbonate mineral and alteration mineral. Variations in the contents of garnet, opaque mineral, and hornblende mainly reflected the degree of sedimentary differentiation in suspended sediment and the hydrodynamic intensity of a drainage system. The heavy mineral differentiation index F revealed sedimentary differentiation of diagnostic detrital mineral composition due to changes in regional hydrodynamic intensity and can serve as an indicator for studying the dynamic sedimentary environment of a single-provenance river and the degree of sedimentary differentiation of its detrital minerals. Changes in detrital mineral content of the Yellow River was not completely controlled by provenance but reflected gravity sorting of the detrital mineral due to variations in the ephemeral river hydrodynamic intensity and sedimentary environment, however the index changing of Yangtze River were mainly influenced by the complex sediment sources. Therefore caution must be exercised in using the detrital mineral composition of marginal sea to determine the contribution of the Yangtze River and Yellow River.展开更多
Gold,iron,copper,lead-zinc and other mineral exploration in West Tianshan,Xinjiang Uygur Autonomous Region,has made remarkable progress in recent years.However,due to the dispute on the tectonic division of West Tians...Gold,iron,copper,lead-zinc and other mineral exploration in West Tianshan,Xinjiang Uygur Autonomous Region,has made remarkable progress in recent years.However,due to the dispute on the tectonic division of West Tianshan,the ore-controlling factors and the regional metallogenic laws are controversial.The authors analyze regional gravity data and notice that the high-value region corresponds to the Yili ancient continent,thus the southeastern boundary of the Yili ancient continent is delineated.Comparative analysis of gravity,aeromagnetic and geologic data reveals that the Tulasu basin,where some medium to large epithermal gold deposits locate,lies above the Yili ancient continent;the Yili Carboniferous-Permian rift extends in E-W direction,numbers of copper deposits have been found in the mid-west section of the rift which lies above the Yili ancient continent,whereas few copper deposits have been discovered in the east section which is outside the Yili ancient continent.Accordingly,the Yili ancient continent may be rich in gold,copper and other metal elements;the metal-bearing hydrothermal solution moves up with the activity of magmatism,and deposits in the favorable places(the Tulasu basin and the Yili Carboniferous-Permian rift),forming numerous small and medium gold,copper deposits,as well as some large and super-large gold deposits.Therefore,the tectonic-magmatic hydrothermal zone above the Yili ancient continent should be the prospective area for epithermal gold and copper polymetallic deposits.展开更多
Zhenjiang has always been a city of heroes,where revolutionary heroes shed their blood and red genes are passed down from generation to generation.In terms of the Zhenjiang region,a hundred years of struggle to cast ...Zhenjiang has always been a city of heroes,where revolutionary heroes shed their blood and red genes are passed down from generation to generation.In terms of the Zhenjiang region,a hundred years of struggle to cast the“Iron Army Spirit”of the New Fourth Army in Mao Mountain Anti-Japanese Base Area,the“Yafu Spirit”that lived up to the people’s expectations of fighting for the city’s glory and poverty alleviation,and the“war spirit”of southern Jiangsu,which was first encountered under Zhenjiang City and was decapitated by the small Lou Lan and other“Zhenjiang symbols”series of Chinese Communist Party spiritual spectrum.The red elements of“Zhenjiang symbols”consists of the blood,tears,and wisdom of the revolutionary predecessors,and shine with the glorious deeds of revolutionary battles.Compared with other schools,vocational colleges are colleges with applied application,practical,and close to nature.How to integrate the red elements of“Zhenjiang symbols”into the classroom of local vocational colleges and a give role to the function of red education has become an important subject of ideological and political education in vocational colleges.展开更多
Tin(IV)oxide(Sn_(3)O_(4))is layered tin and exhibits mixed valence states.It has emerged as a highly promising visible-light pho-tocatalyst,attracting considerable attention.This comprehensive review is aimed at provi...Tin(IV)oxide(Sn_(3)O_(4))is layered tin and exhibits mixed valence states.It has emerged as a highly promising visible-light pho-tocatalyst,attracting considerable attention.This comprehensive review is aimed at providing a detailed overview of the latest advance-ments in research,applications,advantages,and challenges associated with Sn_(3)O_(4)photocatalytic nanomaterials.The fundamental con-cepts and principles of Sn_(3)O_(4)are introduced.Sn_(3)O_(4)possesses a unique crystal structure and optoelectronic properties that allow it to ab-sorb visible light efficiently and generate photoexcited charge carriers that drive photocatalytic reactions.Subsequently,strategies for the control and improved performance of Sn_(3)O_(4)photocatalytic nanomaterials are discussed.Morphology control,ion doping,and hetero-structure construction are widely employed in the optimization of the photocatalytic performance of Sn_(3)O_(4)materials.The effective imple-mentation of these strategies improves the photocatalytic activity and stability of Sn_(3)O_(4)nanomaterials.Furthermore,the review explores the diverse applications of Sn_(3)O_(4)photocatalytic nanomaterials in various fields,such as photocatalytic degradation,photocatalytic hydro-gen production,photocatalytic reduction of carbon dioxide,solar cells,photocatalytic sterilization,and optoelectronic sensors.The discus-sion focuses on the potential of Sn_(3)O_(4)-based nanomaterials in these applications,highlighting their unique attributes and functionalities.Finally,the review provides an outlook on the future development directions in the field and offers guidance for the exploration and de-velopment of novel and efficient Sn_(3)O_(4)-based nanomaterials.Through the identification of emerging research areas and potential avenues for improvement,this review aims to stimulate further advancements in Sn_(3)O_(4)-based photocatalysis and facilitate the translation of this promising technology into practical applications.展开更多
AIM:To explore the usage of choroidal thickness measured by swept-source optical coherence tomography(SS-OCT)to detect myopic macular degeneration(MMD)in high myopic participants.METHODS:Participants with bilateral hi...AIM:To explore the usage of choroidal thickness measured by swept-source optical coherence tomography(SS-OCT)to detect myopic macular degeneration(MMD)in high myopic participants.METHODS:Participants with bilateral high myopia(≤−6 diopters)were recruited from a subset of the Guangzhou Zhongshan Ophthalmic Center-Brien Holden Vision Institute High Myopia Cohort Study.SS-OCT was performed to determine the choroidal thickness,and myopic maculopathy was graded by the International Meta-Analysis for Pathologic Myopia(META-PM)Classification.Presence of MMD was defined as META-PM category 2 or above.RESULTS:A total of 568 right eyes were included for analysis.Eyes with MMD(n=106,18.7%)were found to have older age,longer axial lengths(AL),higher myopic spherical equivalents(SE),and reduced choroidal thickness in each Early Treatment Diabetic Retinopathy Study(ETDRS)grid sector(P<0.001).The area under the receiver operating characteristic(ROC)curves(AUC)for subfoveal choroidal thickness(0.907)was greater than that of the model,including age,AL,and SE at 0.6249,0.8208,and 0.8205,respectively.The choroidal thickness of the inner and outer nasal sectors was the most accurate indicator of MMD(AUC of 0.928 and 0.923,respectively).An outer nasal sector choroidal thickness of less than 74μm demonstrated the highest odds of predicting MMD(OR=33.8).CONCLUSION:Choroidal thickness detects the presence of MMD with high agreement,particularly of the inner and outer nasal sectors of the posterior pole,which appears to be a biometric parameter more precise than age,AL,or SE.展开更多
Free amino acid(FAA)is the important component of vinegar that infl uences quality perception and consumer acceptance.FAA is one of the major metabolites produced by microorganisms;however,the microbial metabolic netw...Free amino acid(FAA)is the important component of vinegar that infl uences quality perception and consumer acceptance.FAA is one of the major metabolites produced by microorganisms;however,the microbial metabolic network on FAA biosynthesis remains unclear.Through metagenomic analysis,this work aimed to elucidate the roles of microbes in FAA biosynthesis during Monascus rice vinegar fermentation.Taxonomic profiles from functional analyses showed 14 dominant genera with high contributions to the metabolism pathways.The metabolic network for FAA biosynthesis was then constructed,and the microbial distribution in different metabolic pathways was illuminated.The results revealed that 5 functional genera were closely involved in FAA biosynthesis.This study illuminated the metabolic roles of microorganisms in FAA biosynthesis and provided crucial insights into the functional attributes of microbiota in vinegar fermentation.展开更多
BACKGROUND Uridine diphosphate glucuronosyltransferase 1A1(UGT1A1)plays a crucial role in metabolizing and detoxifying endogenous and exogenous substances.However,its contribution to the progression of liver damage re...BACKGROUND Uridine diphosphate glucuronosyltransferase 1A1(UGT1A1)plays a crucial role in metabolizing and detoxifying endogenous and exogenous substances.However,its contribution to the progression of liver damage remains unclear.AIM To determine the role and mechanism of UGT1A1 in liver damage progression.METHODS We investigated the relationship between UGT1A1 expression and liver injury through clinical research.Additionally,the impact and mechanism of UGT1A1 on the progression of liver injury was analyzed through a mouse model study.RESULTS Patients with UGT1A1 gene mutations showed varying degrees of liver damage,while patients with acute-onchronic liver failure(ACLF)exhibited relatively reduced levels of UGT1A1 protein in the liver as compared to patients with chronic hepatitis.This suggests that low UGT1A1 levels may be associated with the progression of liver damage.In mouse models of liver injury induced by carbon tetrachloride(CCl_(4))and concanavalin A(ConA),the hepatic levels of UGT1A1 protein were found to be increased.In mice with lipopolysaccharide or liver steatosis-mediated liver-injury progression,the hepatic protein levels of UGT1A1 were decreased,which is consistent with the observations in patients with ACLF.UGT1A1 knockout exacerbated CCl_(4)-and ConA-induced liver injury,hepatocyte apoptosis and necroptosis in mice,intensified hepatocyte endoplasmic reticulum(ER)stress and oxidative stress,and disrupted lipid metabolism.CONCLUSION UGT1A1 is upregulated as a compensatory response during liver injury,and interference with this upregulation process may worsen liver injury.UGT1A1 reduces ER stress,oxidative stress,and lipid metabolism disorder,thereby mitigating hepatocyte apoptosis and necroptosis.展开更多
BACKGROUND Laparoscopic radical gastrectomy is widely used,and perioperative complications have become a highly concerned issue.AIM To develop a predictive model for complications in laparoscopic radical gastrectomy f...BACKGROUND Laparoscopic radical gastrectomy is widely used,and perioperative complications have become a highly concerned issue.AIM To develop a predictive model for complications in laparoscopic radical gastrectomy for gastric cancer to better predict the likelihood of complications in gastric cancer patients within 30 days after surgery,guide perioperative treatment strategies for gastric cancer patients,and prevent serious complications.METHODS In total,998 patients who underwent laparoscopic radical gastrectomy for gastric cancer at 16 Chinese medical centers were included in the training group for the complication model,and 398 patients were included in the validation group.The clinicopathological data and 30-d postoperative complications of gastric cancer patients were collected.Three machine learning methods,lasso regression,random forest,and artificial neural networks,were used to construct postoperative complication prediction models for laparoscopic distal gastrectomy and laparoscopic total gastrectomy,and their prediction efficacy and accuracy were evaluated.RESULTS The constructed complication model,particularly the random forest model,could better predict serious complications in gastric cancer patients undergoing laparoscopic radical gastrectomy.It exhibited stable performance in external validation and is worthy of further promotion in more centers.CONCLUSION Using the risk factors identified in multicenter datasets,highly sensitive risk prediction models for complications following laparoscopic radical gastrectomy were established.We hope to facilitate the diagnosis and treatment of preoperative and postoperative decision-making by using these models.展开更多
基金supported by the Collaborative Innovation Center for Clinical and Translational Science by Chinese Ministry of Education&Shanghai,No.CCTS-2022205the“Double World-Class Project”of Shanghai Jiaotong University School of Medicine(both to JZ)。
文摘Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle weakness.It is one of the most common genetic causes of mortality among infants aged less than 2 years.Biomarker research is currently receiving more attention,and new candidate biomarkers are constantly being discovered.This review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and cons.We also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific biomarkers.This review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future research.Furthermore,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.
基金supported by the Science and Technology(S&T)Program of Hebei Province,No.22377798D(to YZ).
文摘Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease progression.The proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of conditions.In this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s disease.Using a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western blot.The results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease severity.Furthermore,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control cells.Additionally,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated cells.Taken together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced neurotoxicity,thus providing a new therapeutic target for Parkinson’s disease.
文摘BACKGROUND Flat bone metastases are common in patients with advanced cancers,often resulting in severe pain,limited mobility,and reduced quality of life(QOL).Traditional treatment options,such as radiotherapy or systemic therapies,often fail to provide sufficient pain relief or improve functional outcomes in these patients.Microwave ablation(MWA)offers advantages,such as shorter pro-cedure times and larger ablation zones,while percutaneous osteoplasty(PO)enhances bone stability and prevents pathological fractures.Despite these be-nefits,the combination of these techniques for treating flat bone metastases re-mains underexplored.AIM To evaluate the efficacy and safety of C-arm computed tomography(CT)-guided MWA combined with PO for managing painful flat bone metastases,focusing on pain relief,functional improvement,and QOL enhancement.METHODS A total of 45 patients with refractory moderate-to-severe pain resulting from flat bone metastases who underwent C-arm CT-guided MWA combined with PO between January 2015 and January 2021 were included.The efficacy of the pro-cedure was assessed by changes in the visual analog scale(VAS),Oswestry disability index(ODI),and QOL,as well as the occurrence of complications.Tumor response was evaluated using RECIST v1.1 and mRECIST criteria,with overall response rate(ORR)and disease control rate(DCR)as the primary end-points.RESULTS No serious complications were observed in any of the patients.A significant reduction in VAS and ODI was noted at 1 week,1 month,and 3 months post-procedure.A marked improvement in QOL was observed at all follow-up points.Bone cement extravasation was observed in 10 patients;however,none exhibited significant clinical symptoms.Based on RECIST v1.1,the ORR was 26.7%and the DCR was 88.9%.The mRECIST evaluation revealed a higher ORR of 51.1%and DCR of 88.9%.CONCLUSION C-arm CT-guided MWA with PO provides a dependable and effective strategy for managing flat bone metastases.It demonstrates significant pain relief,improved functional outcomes,and enhanced QOL.This treatment combination also shows promising tumor response rates with a low complication profile.
基金supported by the Innovation Program of Shanghai Municipal Education Commission(No.2023ZKZD36).
文摘We are now in the era of big data for biodiversity science.More and larger datasets on species geographic distributions,community composition,and functional traits are now becoming more increasingly than ever before.Correctly applying taxon names is a prerequisite for robust biodiversity studies of all taxonomic groups.
基金supported by grants from the Key R&D Program of Shandong Province (2021ZLGX02)the National Science Foundation of China (81901934, 82325031)+1 种基金the National Key R&D Program of China (2020YFC1512700, 2020YFC1512705, 2020YFC1512703)the Clinical Research Center of Shandong University (2020SDUCRCC025)。
文摘The incidence of in-hospital cardiac arrest (IHCA) has increased over the past decade,with more than half occurring in intensive care units (ICUs).^([1])ICU cardiac arrest (ICU-CA)presents unique challenges,with worse outcomes than those in monitored wards,highlighting the need for early detection and intervention.^([2])Up to 80%of patients exhibit signs of deterioration hours before IHCA.^([3])Although early warning scores based on vital signs are useful,their eff ectiveness in ICUs is limited due to abnormal physiological parameters.^([4])Laboratory markers,such as sodium,potassium,and lactate,are predictive of poor outcomes,^([5])but static measurements may not capture the patient’s trajectory.Trends in laboratory indicators,such as variability and extremes,may offer better predictive value.^([6])This study aimed to evaluate ICU-CA predictive factors,with a focus on vital signs and trends of laboratory indicators.
基金supported by the Genetic analysis of important quality and traits of ginseng and basic research on molecular design breeding(Grant No.U21A20405)。
文摘Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).
基金jointly supported by the National Natural Science Foundation of China (41876059, 41730531)China Geological Survey (GZH201400201, DD20160137, DD20190208, DD20190377)+1 种基金China-ASEAN Maritime Cooperation Fund (121201005000151110)Open Found of Shandong Provincial Key Laboratory of Depositional Mineralization&Sedimentary Mineral (DMSM2017024).
文摘A comparative comparative study on the detrital mineral composition of stream sediments of the Yangtze River (Changjiang) and Yellow River (Huanghe) shows that, light minerals of the Yangtze River basin were mainly quartz, feldspar, and detritus, the compositional characteristics of light minerals differed among tributaries, the main stream had a generally higher maturity index than tributaries;heavy mineral content tended to decrease progressively from the upper stream to lower stream of the Yangtze River, the primary assemblage was magnetite-hornblende-augite-garnet-epidote, and diagnostic minerals of different river basins were capable of indicating the nature and distribution of the source rock. Detrital mineral assemblages in sediments of tributaries and the main stream of the Yellow River were basically similar, Primary heavy mineral assemblage was opaque mineral-garnet-epidote-carbonate mineral and alteration mineral. Variations in the contents of garnet, opaque mineral, and hornblende mainly reflected the degree of sedimentary differentiation in suspended sediment and the hydrodynamic intensity of a drainage system. The heavy mineral differentiation index F revealed sedimentary differentiation of diagnostic detrital mineral composition due to changes in regional hydrodynamic intensity and can serve as an indicator for studying the dynamic sedimentary environment of a single-provenance river and the degree of sedimentary differentiation of its detrital minerals. Changes in detrital mineral content of the Yellow River was not completely controlled by provenance but reflected gravity sorting of the detrital mineral due to variations in the ephemeral river hydrodynamic intensity and sedimentary environment, however the index changing of Yangtze River were mainly influenced by the complex sediment sources. Therefore caution must be exercised in using the detrital mineral composition of marginal sea to determine the contribution of the Yangtze River and Yellow River.
基金This research is financially supported by China Geological Survey project“Comprehensive Monitoring of Resources and Environment Bearing Capacity and Digital Platform Construction of Xiongan New Area”(121201003000172401)“Aero Geophysical Survey of Qinling and Tianshan metallogenetic belt”(121201003000150006).
文摘Gold,iron,copper,lead-zinc and other mineral exploration in West Tianshan,Xinjiang Uygur Autonomous Region,has made remarkable progress in recent years.However,due to the dispute on the tectonic division of West Tianshan,the ore-controlling factors and the regional metallogenic laws are controversial.The authors analyze regional gravity data and notice that the high-value region corresponds to the Yili ancient continent,thus the southeastern boundary of the Yili ancient continent is delineated.Comparative analysis of gravity,aeromagnetic and geologic data reveals that the Tulasu basin,where some medium to large epithermal gold deposits locate,lies above the Yili ancient continent;the Yili Carboniferous-Permian rift extends in E-W direction,numbers of copper deposits have been found in the mid-west section of the rift which lies above the Yili ancient continent,whereas few copper deposits have been discovered in the east section which is outside the Yili ancient continent.Accordingly,the Yili ancient continent may be rich in gold,copper and other metal elements;the metal-bearing hydrothermal solution moves up with the activity of magmatism,and deposits in the favorable places(the Tulasu basin and the Yili Carboniferous-Permian rift),forming numerous small and medium gold,copper deposits,as well as some large and super-large gold deposits.Therefore,the tectonic-magmatic hydrothermal zone above the Yili ancient continent should be the prospective area for epithermal gold and copper polymetallic deposits.
基金supported by Zhenjiang City 2022 Annual Social Science Applied Research Project(Project Number:2022jy043).
文摘Zhenjiang has always been a city of heroes,where revolutionary heroes shed their blood and red genes are passed down from generation to generation.In terms of the Zhenjiang region,a hundred years of struggle to cast the“Iron Army Spirit”of the New Fourth Army in Mao Mountain Anti-Japanese Base Area,the“Yafu Spirit”that lived up to the people’s expectations of fighting for the city’s glory and poverty alleviation,and the“war spirit”of southern Jiangsu,which was first encountered under Zhenjiang City and was decapitated by the small Lou Lan and other“Zhenjiang symbols”series of Chinese Communist Party spiritual spectrum.The red elements of“Zhenjiang symbols”consists of the blood,tears,and wisdom of the revolutionary predecessors,and shine with the glorious deeds of revolutionary battles.Compared with other schools,vocational colleges are colleges with applied application,practical,and close to nature.How to integrate the red elements of“Zhenjiang symbols”into the classroom of local vocational colleges and a give role to the function of red education has become an important subject of ideological and political education in vocational colleges.
基金the financial support for this work from the National Natural Science Foundation of China(No.52205334)the Natural Science Foundation of Hunan Province,China(No.2022JJ40495)+2 种基金the Changsha Key Research and Development Project,China(No.kh2201275)the Changsha Municipal Natural Science Foundation,China(No.kq2202196)the Tribology Science Fund of State Key Laboratory of Tribology in Advanced Equipment,China(No.SKLTKF21B08)。
基金the National Natural Science Foundation of China(No.52272212)the Natural Science Foundation of Shandong Province(Nos.ZR2022JQ20 and ZR2023MB126)+2 种基金the Taishan Scholar Project of Shandong Province(No.tsqn202211168)the Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE(No.M2022-7)the STIEI scientific research funding project(No.GCC2023036).
文摘Tin(IV)oxide(Sn_(3)O_(4))is layered tin and exhibits mixed valence states.It has emerged as a highly promising visible-light pho-tocatalyst,attracting considerable attention.This comprehensive review is aimed at providing a detailed overview of the latest advance-ments in research,applications,advantages,and challenges associated with Sn_(3)O_(4)photocatalytic nanomaterials.The fundamental con-cepts and principles of Sn_(3)O_(4)are introduced.Sn_(3)O_(4)possesses a unique crystal structure and optoelectronic properties that allow it to ab-sorb visible light efficiently and generate photoexcited charge carriers that drive photocatalytic reactions.Subsequently,strategies for the control and improved performance of Sn_(3)O_(4)photocatalytic nanomaterials are discussed.Morphology control,ion doping,and hetero-structure construction are widely employed in the optimization of the photocatalytic performance of Sn_(3)O_(4)materials.The effective imple-mentation of these strategies improves the photocatalytic activity and stability of Sn_(3)O_(4)nanomaterials.Furthermore,the review explores the diverse applications of Sn_(3)O_(4)photocatalytic nanomaterials in various fields,such as photocatalytic degradation,photocatalytic hydro-gen production,photocatalytic reduction of carbon dioxide,solar cells,photocatalytic sterilization,and optoelectronic sensors.The discus-sion focuses on the potential of Sn_(3)O_(4)-based nanomaterials in these applications,highlighting their unique attributes and functionalities.Finally,the review provides an outlook on the future development directions in the field and offers guidance for the exploration and de-velopment of novel and efficient Sn_(3)O_(4)-based nanomaterials.Through the identification of emerging research areas and potential avenues for improvement,this review aims to stimulate further advancements in Sn_(3)O_(4)-based photocatalysis and facilitate the translation of this promising technology into practical applications.
基金Supported by the National Natural Science Foundation of China(No.82301249,No.82371086)the Science and Technology Projects in Guangzhou(No.SL2024A04J01756)the Fundamental Research Funds of the State Key Laboratory of Ophthalmology(No.83000-32030003).
文摘AIM:To explore the usage of choroidal thickness measured by swept-source optical coherence tomography(SS-OCT)to detect myopic macular degeneration(MMD)in high myopic participants.METHODS:Participants with bilateral high myopia(≤−6 diopters)were recruited from a subset of the Guangzhou Zhongshan Ophthalmic Center-Brien Holden Vision Institute High Myopia Cohort Study.SS-OCT was performed to determine the choroidal thickness,and myopic maculopathy was graded by the International Meta-Analysis for Pathologic Myopia(META-PM)Classification.Presence of MMD was defined as META-PM category 2 or above.RESULTS:A total of 568 right eyes were included for analysis.Eyes with MMD(n=106,18.7%)were found to have older age,longer axial lengths(AL),higher myopic spherical equivalents(SE),and reduced choroidal thickness in each Early Treatment Diabetic Retinopathy Study(ETDRS)grid sector(P<0.001).The area under the receiver operating characteristic(ROC)curves(AUC)for subfoveal choroidal thickness(0.907)was greater than that of the model,including age,AL,and SE at 0.6249,0.8208,and 0.8205,respectively.The choroidal thickness of the inner and outer nasal sectors was the most accurate indicator of MMD(AUC of 0.928 and 0.923,respectively).An outer nasal sector choroidal thickness of less than 74μm demonstrated the highest odds of predicting MMD(OR=33.8).CONCLUSION:Choroidal thickness detects the presence of MMD with high agreement,particularly of the inner and outer nasal sectors of the posterior pole,which appears to be a biometric parameter more precise than age,AL,or SE.
基金The authors are grateful for the financial support from National Natural Science Foundation of China(32001728).
文摘Free amino acid(FAA)is the important component of vinegar that infl uences quality perception and consumer acceptance.FAA is one of the major metabolites produced by microorganisms;however,the microbial metabolic network on FAA biosynthesis remains unclear.Through metagenomic analysis,this work aimed to elucidate the roles of microbes in FAA biosynthesis during Monascus rice vinegar fermentation.Taxonomic profiles from functional analyses showed 14 dominant genera with high contributions to the metabolism pathways.The metabolic network for FAA biosynthesis was then constructed,and the microbial distribution in different metabolic pathways was illuminated.The results revealed that 5 functional genera were closely involved in FAA biosynthesis.This study illuminated the metabolic roles of microorganisms in FAA biosynthesis and provided crucial insights into the functional attributes of microbiota in vinegar fermentation.
基金the Science and Technology Research Foundations of Guizhou Province,No.QKHJC-ZK(2022)YB642Zunyi Science and Technology Plan Project,No.ZSKHHZ(2022)344,No.ZSKHHZ(2022)360,and No.ZYK160+2 种基金Hubei Province Central Leading Local Science and Technology Development Special Project,No.2022BCE030Changzhou Science and Technology Projects,No.CE20225054Bijie City Science and Planning Bureau,No.BKH(2022)8.
文摘BACKGROUND Uridine diphosphate glucuronosyltransferase 1A1(UGT1A1)plays a crucial role in metabolizing and detoxifying endogenous and exogenous substances.However,its contribution to the progression of liver damage remains unclear.AIM To determine the role and mechanism of UGT1A1 in liver damage progression.METHODS We investigated the relationship between UGT1A1 expression and liver injury through clinical research.Additionally,the impact and mechanism of UGT1A1 on the progression of liver injury was analyzed through a mouse model study.RESULTS Patients with UGT1A1 gene mutations showed varying degrees of liver damage,while patients with acute-onchronic liver failure(ACLF)exhibited relatively reduced levels of UGT1A1 protein in the liver as compared to patients with chronic hepatitis.This suggests that low UGT1A1 levels may be associated with the progression of liver damage.In mouse models of liver injury induced by carbon tetrachloride(CCl_(4))and concanavalin A(ConA),the hepatic levels of UGT1A1 protein were found to be increased.In mice with lipopolysaccharide or liver steatosis-mediated liver-injury progression,the hepatic protein levels of UGT1A1 were decreased,which is consistent with the observations in patients with ACLF.UGT1A1 knockout exacerbated CCl_(4)-and ConA-induced liver injury,hepatocyte apoptosis and necroptosis in mice,intensified hepatocyte endoplasmic reticulum(ER)stress and oxidative stress,and disrupted lipid metabolism.CONCLUSION UGT1A1 is upregulated as a compensatory response during liver injury,and interference with this upregulation process may worsen liver injury.UGT1A1 reduces ER stress,oxidative stress,and lipid metabolism disorder,thereby mitigating hepatocyte apoptosis and necroptosis.
基金Natural Science Foundation of Fujian Province,No.2021J011360,and No.2020J011230Natural Science Foundation of Xiamen,China,No.3502Z20214ZD1018,and No.3502Z20227096+2 种基金Medical Innovation Project of Fujian Provincial Health Commission,No.2021CXB019Youth Scientific Research Project of Fujian Provincial Health Commission,No.2022QNB013Bethune Charitable Foundation,No.HZB-20190528-10.
文摘BACKGROUND Laparoscopic radical gastrectomy is widely used,and perioperative complications have become a highly concerned issue.AIM To develop a predictive model for complications in laparoscopic radical gastrectomy for gastric cancer to better predict the likelihood of complications in gastric cancer patients within 30 days after surgery,guide perioperative treatment strategies for gastric cancer patients,and prevent serious complications.METHODS In total,998 patients who underwent laparoscopic radical gastrectomy for gastric cancer at 16 Chinese medical centers were included in the training group for the complication model,and 398 patients were included in the validation group.The clinicopathological data and 30-d postoperative complications of gastric cancer patients were collected.Three machine learning methods,lasso regression,random forest,and artificial neural networks,were used to construct postoperative complication prediction models for laparoscopic distal gastrectomy and laparoscopic total gastrectomy,and their prediction efficacy and accuracy were evaluated.RESULTS The constructed complication model,particularly the random forest model,could better predict serious complications in gastric cancer patients undergoing laparoscopic radical gastrectomy.It exhibited stable performance in external validation and is worthy of further promotion in more centers.CONCLUSION Using the risk factors identified in multicenter datasets,highly sensitive risk prediction models for complications following laparoscopic radical gastrectomy were established.We hope to facilitate the diagnosis and treatment of preoperative and postoperative decision-making by using these models.
