Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,a...Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection.WB test was used to measure the levels of cyclinD1,p27 and FOXO3.Meanwhile.QPCR was used to detect miR-184expression in glioma cell line.glioma tissues and adjacent tissues.Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3.Results:QPCR results showed a significaat lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue.MTT and plate cloning experiments have shown that after overexpressing of miR-184,the cell proliferation capacity of glioma U87 and T98G was signifieantly increased,which was significantly inhibited after the inhibition of miN-184.WB results showed a lower expression level of p27 in U87 and T98G cells,and a higher expression level of cyclind1after over-expressing of miR-184 wss observed.However.a lower expression level of eyelinD1and a higher expression level of p27 after the inhibition of miR-184.The luciferase activity was inhibited after the over-expressing of miR-184.Conclusions:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein.It plays an important role in the occurrence and development of gliomas.展开更多
There are some curved interfaces in ocean acoustic waveguides. To compute wave propagation along the range with some marching methods, a flattening of the internal interfaces and a transforming equation are needed. In...There are some curved interfaces in ocean acoustic waveguides. To compute wave propagation along the range with some marching methods, a flattening of the internal interfaces and a transforming equation are needed. In this paper a local orthogonal coordinate transform and an equation transformation are constructed to flatten interfaces and change the Helmholtz equation as a solvable form. For a waveguide with a flat top, a fiat bottom and n curved interfaces, the coefficients of the transformed Helmholtz equation are given in a closed formulation which can be thought of as an extension of the formal work related to the equation transformation with two curved internal interfaces. In the transformed horizontally stratified waveguide, the one-way reformulation based on the Dirichlet-to-Neumann (DtN) map is then used to reduce the boundary value problem to an initial value problem. Numerical implementation of the resulting operator Riccati equation uses a large range step method to discretize the range variable and a truncated local eigenfunction expansion to approximate the operators. This method is particularly useful for solving long range wave propagation problems in slowly varying waveguides. Furthermore, the method can also be applied to wave propagation problems in acoustic waveguides associated with varied density.展开更多
AIM: To study the morphology and ontogeny of dendritic cells of Peyer's patches in rats at different development periods. METHODS: The morphometric and flow cytometric analyses were performed to detect all the para...AIM: To study the morphology and ontogeny of dendritic cells of Peyer's patches in rats at different development periods. METHODS: The morphometric and flow cytometric analyses were performed to detect all the parameters of villous-crypts axis and the number of OX62+DC, OX62+CD4+SIRP+DC, and OX62+CD4-SIRP-DC in the small intestine in different groups of rats. The relationship between the parameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS: All morphometric parameters changed significantly with the development of pups in the different age groups (F = 10.751, 12.374, 16.527, 5.291, 3.486; P = 0.000, 0.000, 0.000, 0.001, 0.015). Villous height levels were unstable and increased from 115.24μm to 140.43 μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum, peeked up to 13817.60 tam2 at 7 wk postpartum. Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80 + 1.01 to 4.54 =1= 1.56, 9-11 wk postpartum. The expression of OX62+DC increased from 33.30%±5.80% to 80%± 17.30%, 3-11 wk postpartum (F =5.536, P = 0.0013). OX62+CD4+SIRP+DC subset levels detected in single-cell suspensions of rat total Peyer's patch dendritic cells (PP-DCs) increased significantly from 30.73% ± 5.16% to 35.50% ± 4.08%, 5-7 wk postpartum and from 34.20% ±1.35% to 43.60% ± 2.07% 9-11 wk postpartum (F = 7.216, P = 0.005). CONCLUSION: This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine. Simultaneously, there are also development and maturation in rat PP-DCs phenotypic expression. Furthermore, the morphological changes of intestinal mucosa and the development of immune cells (especially DC) peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.展开更多
Modeling of f-electron systems is challenging due to the complex interplay of the effects of spin–orbit coupling,electron–electron interactions,and the hybridization of the localized f-electrons with itinerant condu...Modeling of f-electron systems is challenging due to the complex interplay of the effects of spin–orbit coupling,electron–electron interactions,and the hybridization of the localized f-electrons with itinerant conduction electrons.This complexity drives not only the richness of electronic properties but also makes these materials suitable for diverse technological applications.In this context,we propose and implement a data-driven approach to aid the materials discovery process.By deploying state-of-the-art algorithms and query tools,we train our learning models using a large,simulated dataset based on existing actinide and lanthanide compounds.The machine-learned models so obtained can then be used to search for new classes of stable materials with desired electronic and physical properties.We discuss the basic structure of our f-electron database,and our approach towards cleaning and correcting the structure data files.Illustrative examples of the applications of our database include successful prediction of stable superstructures of double perovskites and identification of a number of physically-relevant trends in strongly correlated features of f-electron based materials.展开更多
In this paper, the optical wave propagation in lossy waveguides is described by the Helmholtz equation with the complex refractive-index, and the Chebyshev pseudospectral method is used to discretize the transverse op...In this paper, the optical wave propagation in lossy waveguides is described by the Helmholtz equation with the complex refractive-index, and the Chebyshev pseudospectral method is used to discretize the transverse operator of the equation. Meanwhile, an operator marching method, a one-way re-formulation based on the Dirichlet- to-Neumann (DtN) map, is improved to solve the equation. NumericM examples show that our treatment is more efficient.展开更多
基金supported by National Science Foundation(Grant No.81372721)
文摘Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection.WB test was used to measure the levels of cyclinD1,p27 and FOXO3.Meanwhile.QPCR was used to detect miR-184expression in glioma cell line.glioma tissues and adjacent tissues.Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3.Results:QPCR results showed a significaat lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue.MTT and plate cloning experiments have shown that after overexpressing of miR-184,the cell proliferation capacity of glioma U87 and T98G was signifieantly increased,which was significantly inhibited after the inhibition of miN-184.WB results showed a lower expression level of p27 in U87 and T98G cells,and a higher expression level of cyclind1after over-expressing of miR-184 wss observed.However.a lower expression level of eyelinD1and a higher expression level of p27 after the inhibition of miR-184.The luciferase activity was inhibited after the over-expressing of miR-184.Conclusions:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein.It plays an important role in the occurrence and development of gliomas.
基金the National Natural Science Foundation of China (No. 10571162)the Natural Science Foundation of Zheji-ang Province, China (No. Y605181)
文摘There are some curved interfaces in ocean acoustic waveguides. To compute wave propagation along the range with some marching methods, a flattening of the internal interfaces and a transforming equation are needed. In this paper a local orthogonal coordinate transform and an equation transformation are constructed to flatten interfaces and change the Helmholtz equation as a solvable form. For a waveguide with a flat top, a fiat bottom and n curved interfaces, the coefficients of the transformed Helmholtz equation are given in a closed formulation which can be thought of as an extension of the formal work related to the equation transformation with two curved internal interfaces. In the transformed horizontally stratified waveguide, the one-way reformulation based on the Dirichlet-to-Neumann (DtN) map is then used to reduce the boundary value problem to an initial value problem. Numerical implementation of the resulting operator Riccati equation uses a large range step method to discretize the range variable and a truncated local eigenfunction expansion to approximate the operators. This method is particularly useful for solving long range wave propagation problems in slowly varying waveguides. Furthermore, the method can also be applied to wave propagation problems in acoustic waveguides associated with varied density.
基金Supported by Grants from the National Natural Science Foundation of China,No.30571979
文摘AIM: To study the morphology and ontogeny of dendritic cells of Peyer's patches in rats at different development periods. METHODS: The morphometric and flow cytometric analyses were performed to detect all the parameters of villous-crypts axis and the number of OX62+DC, OX62+CD4+SIRP+DC, and OX62+CD4-SIRP-DC in the small intestine in different groups of rats. The relationship between the parameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS: All morphometric parameters changed significantly with the development of pups in the different age groups (F = 10.751, 12.374, 16.527, 5.291, 3.486; P = 0.000, 0.000, 0.000, 0.001, 0.015). Villous height levels were unstable and increased from 115.24μm to 140.43 μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum, peeked up to 13817.60 tam2 at 7 wk postpartum. Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80 + 1.01 to 4.54 =1= 1.56, 9-11 wk postpartum. The expression of OX62+DC increased from 33.30%±5.80% to 80%± 17.30%, 3-11 wk postpartum (F =5.536, P = 0.0013). OX62+CD4+SIRP+DC subset levels detected in single-cell suspensions of rat total Peyer's patch dendritic cells (PP-DCs) increased significantly from 30.73% ± 5.16% to 35.50% ± 4.08%, 5-7 wk postpartum and from 34.20% ±1.35% to 43.60% ± 2.07% 9-11 wk postpartum (F = 7.216, P = 0.005). CONCLUSION: This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine. Simultaneously, there are also development and maturation in rat PP-DCs phenotypic expression. Furthermore, the morphological changes of intestinal mucosa and the development of immune cells (especially DC) peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.
基金This work is supported by the Institute for Materials Sciences(IMS),NSEC at LANL and by the U.S.D.O.E at LANL under Project No.20170680ER(T.A.)through the LANL LDRD program.Work at LANL was supported in part by U.S.DOE Basic Energy Sciences Core Program LANL E3B5(J.-X.Z.and A.V.B.)The work at Northeastern University is supported by the US Department of Energy,Office of Science,Basic Energy Sciences grant number DE-FG02-07ER46352benefitted from Northeastern University’s Advanced Scientific Computation Center(ASCC)and the NERSC supercomputing center through DOE grant number DE-AC02-05CH11231.
文摘Modeling of f-electron systems is challenging due to the complex interplay of the effects of spin–orbit coupling,electron–electron interactions,and the hybridization of the localized f-electrons with itinerant conduction electrons.This complexity drives not only the richness of electronic properties but also makes these materials suitable for diverse technological applications.In this context,we propose and implement a data-driven approach to aid the materials discovery process.By deploying state-of-the-art algorithms and query tools,we train our learning models using a large,simulated dataset based on existing actinide and lanthanide compounds.The machine-learned models so obtained can then be used to search for new classes of stable materials with desired electronic and physical properties.We discuss the basic structure of our f-electron database,and our approach towards cleaning and correcting the structure data files.Illustrative examples of the applications of our database include successful prediction of stable superstructures of double perovskites and identification of a number of physically-relevant trends in strongly correlated features of f-electron based materials.
基金supported by the National Natural Science Foundation of China(No.11371319)the Zhejiang Provincial Natural Science Foundation of China(No.LY13A010002)
文摘In this paper, the optical wave propagation in lossy waveguides is described by the Helmholtz equation with the complex refractive-index, and the Chebyshev pseudospectral method is used to discretize the transverse operator of the equation. Meanwhile, an operator marching method, a one-way re-formulation based on the Dirichlet- to-Neumann (DtN) map, is improved to solve the equation. NumericM examples show that our treatment is more efficient.