DEAR EDITOR, Despite the vulnerability of primates to the negative impacts of human activities and climate change, there is still room for optimism. Notably, years of conservation efforts may have paid off for the gol...DEAR EDITOR, Despite the vulnerability of primates to the negative impacts of human activities and climate change, there is still room for optimism. Notably, years of conservation efforts may have paid off for the golden snub-nosed monkey(Rhinopithecus roxellana). Our field surveys confirmed the existence of 188 to 220 wild multilevel societies(MLS) of R. roxellana, with an estimated 22 710 to 26 130 individuals in 2019。展开更多
Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatme...Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatment.Staphyloxanthin is a crucial virulence factor of S.aureus.Blocking staphyloxanthin production could help the host immune system counteract the invading S.aureus cells.In this study,we first screened for staphyloxanthin inhibitors using a virtual screening method.The outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in S.aureus ATCC 29213,S.aureus Newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,respectively.The outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of S.aureus.Moreover,the survival rate of S.aureus ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,respectively.The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme.Taken together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S.aureus especially MRSA infections.展开更多
Infected wounds pose a significant global health challenge due to the persistence of bacterial biofilms and limited tissue self-repair.Nitric oxide(NO)functions as a potent antimicrobial agent,demonstrating a dual cap...Infected wounds pose a significant global health challenge due to the persistence of bacterial biofilms and limited tissue self-repair.Nitric oxide(NO)functions as a potent antimicrobial agent,demonstrating a dual capacity for both antimicrobial action and tissue rejuvenation across varying concentrations.However,achieving controlled NO release at distinct stages of infected wound progression,simultaneously targeting biofilm removal and wound recovery,remains a formidable challenge.In this work,we introduce a smart electrospun fibrous membrane,featuring an interior laden with NO-loaded HKUST-1 particles and a porous external surface.Notably,the results reveal the photothermal property of HKUST-1 when exposed to near-infrared(NIR)light,enabling precise management of NO release contingent upon light conditions.During the initial phase of infection treatment,a significant NO release is triggered by near-infrared photothermal stimulation,synergistically complementing photothermal therapy to effectively eliminate bacterial biofilms.Subsequently,in the wound-healing phase,NO is released from the degrading fibrous membrane in a controlled and gradual manner,synergizing with trace amounts of copper ions released during MOF degradation.This collaborative mechanism accelerates the formation of blood vessels within the wound,thereby facilitating the healing process.This study suggests a promising and innovative approach for the effective treatment of infected wounds.展开更多
During the long term evolution of globular clusters(GCs), some member stars are lost to the field. The recently found nitrogen-rich(N-rich) metal-poor field stars are promising candidates of these GC escapees, since N...During the long term evolution of globular clusters(GCs), some member stars are lost to the field. The recently found nitrogen-rich(N-rich) metal-poor field stars are promising candidates of these GC escapees, since N enhancement is the fingerprint of chemically enhanced populations in GCs. In this work, we discuss the possibility of identifying N-rich metal-poor field stars with the upcoming Chinese Space Station Telescope(CSST). We focus on the main survey camera with NUV, u, g, r, i, z, y filters and slitless spectrograph with a resolution about 200.The combination of UV sensitive equipment and prominent N-related molecular lines in the UV band bodes well for the identification: the color–color diagram of(u-g) versus(g-r) is capable of separating N-rich field stars from normal halo stars, if metallicity can be estimated without using the information on u-band photometry.Besides, the synthetic spectra show that a signal-to-noise ratio of 10 is sufficient to identify N-rich field stars. In the near future, a large sample of N-rich field stars found by CSST, combined with state-of-the-art N-body simulations will be crucial to deciphering GC-Galaxy co-evolution.展开更多
Population genomic data could provide valuable information for conservation efforts;however,limited studies have been conducted to investigate the genetic status of threatened pheasants.Reeves’s Pheasant(Syrmaticus r...Population genomic data could provide valuable information for conservation efforts;however,limited studies have been conducted to investigate the genetic status of threatened pheasants.Reeves’s Pheasant(Syrmaticus reevesii)is facing population decline,attributed to increases in habitat loss.There is a knowledge gap in understanding the genomic status and genetic basis underlying the local adaptation of this threatened bird.Here,we used population genomic data to assess population structure,genetic diversity,inbreeding patterns,and genetic divergence.Furthermore,we identified candidate genes linked with adaptation across the current distribution of Reeves’s Pheasant.The present study assembled the first de novo genome sequence of Reeves’s Pheasant and annotated 19,458 genes.We also sequenced 30 individuals from three populations(Dabie Mountain,Shennongjia,Qinling Mountain)and found that there was clear population structure among those populations.By comparing with other threatened species,we found that Reeves’s Pheasants have low genetic diversity.Runs of homozygosity suggest that the Shennongjia population has experienced serious inbreeding.The demographic history results indicated that three populations experienced several declines during the glacial period.Local adaptative analysis among the populations identified 241 candidate genes under directional selection.They are involved in a large variety of processes,including the immune response and pigmentation.Our results suggest that the three populations should be considered as three different conservation units.The current study provides genetic evidence for conserving the threatened Reeves’s Pheasant and provides genomic resources for global biodiversity management.展开更多
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA23080000,XDB31000000,XDA19050000)National Natural Science Foundation of China(31821001,31872248,32070450,32171487,32001097)+2 种基金Ministry of Ecology and Environment of China(2019HB2096001006)Sichuan Science and Technology Program(2021JDRC0024)。
文摘DEAR EDITOR, Despite the vulnerability of primates to the negative impacts of human activities and climate change, there is still room for optimism. Notably, years of conservation efforts may have paid off for the golden snub-nosed monkey(Rhinopithecus roxellana). Our field surveys confirmed the existence of 188 to 220 wild multilevel societies(MLS) of R. roxellana, with an estimated 22 710 to 26 130 individuals in 2019。
基金supported by the National Natural Science Foundation of China (31972169 and 32001798)。
文摘Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatment.Staphyloxanthin is a crucial virulence factor of S.aureus.Blocking staphyloxanthin production could help the host immune system counteract the invading S.aureus cells.In this study,we first screened for staphyloxanthin inhibitors using a virtual screening method.The outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in S.aureus ATCC 29213,S.aureus Newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,respectively.The outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of S.aureus.Moreover,the survival rate of S.aureus ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,respectively.The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme.Taken together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S.aureus especially MRSA infections.
基金supported by the National Natural Science Foundation of China(Grant No.32271386)Zhejiang Engineering Research Center for Tissue Repair Materials(Grant No:WIUCASZZXF21001)+4 种基金Wenzhou Science and Technology Major Project(ZY2022028)Wenzhou Science and Technology Project(Y20220142)the seed grants from the Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2020013,WIUCASQD2021030)the Opening Project of State Key Laboratory of High Performance Ceramics and Superfine Microstructure(Grant Nos:SKL-202112SIC,SKL202213SIC)the founding from First Affiliated Hospital of Wenzhou Medical University.
文摘Infected wounds pose a significant global health challenge due to the persistence of bacterial biofilms and limited tissue self-repair.Nitric oxide(NO)functions as a potent antimicrobial agent,demonstrating a dual capacity for both antimicrobial action and tissue rejuvenation across varying concentrations.However,achieving controlled NO release at distinct stages of infected wound progression,simultaneously targeting biofilm removal and wound recovery,remains a formidable challenge.In this work,we introduce a smart electrospun fibrous membrane,featuring an interior laden with NO-loaded HKUST-1 particles and a porous external surface.Notably,the results reveal the photothermal property of HKUST-1 when exposed to near-infrared(NIR)light,enabling precise management of NO release contingent upon light conditions.During the initial phase of infection treatment,a significant NO release is triggered by near-infrared photothermal stimulation,synergistically complementing photothermal therapy to effectively eliminate bacterial biofilms.Subsequently,in the wound-healing phase,NO is released from the degrading fibrous membrane in a controlled and gradual manner,synergizing with trace amounts of copper ions released during MOF degradation.This collaborative mechanism accelerates the formation of blood vessels within the wound,thereby facilitating the healing process.This study suggests a promising and innovative approach for the effective treatment of infected wounds.
基金support from the China Manned Space Project Nos. CMS-CSST-2021-B03,CMS-CSST-2021-A08, and________the National Natural Science Foundation of China under grant No. 12233013,12073090+8 种基金the Natural Science Foundation of Guangdong Province under grant No. 2022A1515010732grant support provided by Proyecto Fondecyt Iniciación No. 11220340ANID Concurso de Fomento a la Vinculación Internacional para Instituciones de Investigación Regionales (Modalidad corta duración) Proyecto No. FOVI210020Joint Committee ESO-Government of Chile 2021 (ORP 023/2021)Becas Santander Movilidad Internacional Profesores 2022, Banco Santander Chilesupport from the National Natural Science Foundation of China through grant 21BAA00619the one-hundred-talent project of Sun Yat-sen Universitythe Fundamental Research Funds for the Central UniversitiesSun Yat-sen University (22hytd09)。
文摘During the long term evolution of globular clusters(GCs), some member stars are lost to the field. The recently found nitrogen-rich(N-rich) metal-poor field stars are promising candidates of these GC escapees, since N enhancement is the fingerprint of chemically enhanced populations in GCs. In this work, we discuss the possibility of identifying N-rich metal-poor field stars with the upcoming Chinese Space Station Telescope(CSST). We focus on the main survey camera with NUV, u, g, r, i, z, y filters and slitless spectrograph with a resolution about 200.The combination of UV sensitive equipment and prominent N-related molecular lines in the UV band bodes well for the identification: the color–color diagram of(u-g) versus(g-r) is capable of separating N-rich field stars from normal halo stars, if metallicity can be estimated without using the information on u-band photometry.Besides, the synthetic spectra show that a signal-to-noise ratio of 10 is sufficient to identify N-rich field stars. In the near future, a large sample of N-rich field stars found by CSST, combined with state-of-the-art N-body simulations will be crucial to deciphering GC-Galaxy co-evolution.
基金supported by the Biodiversity Survey,Monitoring and Assessment Project(2019–2023)of the Ministry of Ecology and EnvironmentChina(No.2019HB2096001006 to ZZ)+2 种基金the National Natural Science Foundation of China(31672319)Endangered Species Scientific Commission of China(No.2022–331)supported by the China Scholarship Council,China。
文摘Population genomic data could provide valuable information for conservation efforts;however,limited studies have been conducted to investigate the genetic status of threatened pheasants.Reeves’s Pheasant(Syrmaticus reevesii)is facing population decline,attributed to increases in habitat loss.There is a knowledge gap in understanding the genomic status and genetic basis underlying the local adaptation of this threatened bird.Here,we used population genomic data to assess population structure,genetic diversity,inbreeding patterns,and genetic divergence.Furthermore,we identified candidate genes linked with adaptation across the current distribution of Reeves’s Pheasant.The present study assembled the first de novo genome sequence of Reeves’s Pheasant and annotated 19,458 genes.We also sequenced 30 individuals from three populations(Dabie Mountain,Shennongjia,Qinling Mountain)and found that there was clear population structure among those populations.By comparing with other threatened species,we found that Reeves’s Pheasants have low genetic diversity.Runs of homozygosity suggest that the Shennongjia population has experienced serious inbreeding.The demographic history results indicated that three populations experienced several declines during the glacial period.Local adaptative analysis among the populations identified 241 candidate genes under directional selection.They are involved in a large variety of processes,including the immune response and pigmentation.Our results suggest that the three populations should be considered as three different conservation units.The current study provides genetic evidence for conserving the threatened Reeves’s Pheasant and provides genomic resources for global biodiversity management.
