Duchesnea Phenolic Fraction Inhibits Tumor Growth through Restoring the Th1/Th2 Balance in U14 Cervical Cancer Bearing Mice

Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.

Effect of sodium starch octenyl succinate-based Pickering emulsion on the physicochemical properties of hairtail myofibrillar protein gel subjected to multiple freeze-thaw cycles

A Pickering emulsion based on sodium starch octenyl succinate(SSOS)was prepared and its effects on the physicochemical properties of hairtail myofibrillar protein gels(MPGs)subjected to multiple freeze-thaw(F-T)cycles were investigated.The whiteness,water-holding capacity,storage modulus(G')and texture properties of the MPGs were significantly improved by adding 1%-2%Pickering emulsion(P<0.05).Meanwhile,Raman spectral analysis demonstrated that Pickering emulsion promoted the transformation of secondary structure,enhanced hydrogen bonds and hydrophobic interactions,and promoted the transition of disulfide bond conformation from g-g-g to g-g-t and t-g-t.At an emulsion concentration of 2%,theα-helix content decreased by 10.37%,while theβ-sheet content increased by 7.94%,compared to the control.After F-T cycles,the structure of the MPGs was destroyed,with an increase in hardness and a decrease in whiteness and water-holding capacity,however,the quality degradation of MPGs was reduced with 1%-2%Pickering emulsion.These findings demonstrated that SSOS-Pickering emulsions,as potential fat substitutes,can enhance the gel properties and the F-T stability of MPGs.

Virtual screening,molecular docking and identification of umami peptides derived from Oncorhynchus mykiss

Oncorhynchus mykiss is delicious and contains abundant flavor substances.However,few studies focused on umami peptides of O.mykiss.In the current work,umami peptides derived from O.mykiss were identified using virtual screening,molecular docking,and electronic tongue analysis.First,the O.mykiss protein was hydrolyzed using the PeptideCutter online enzymolysis program.Subsequently,water-soluble and toxicity screening were performed by Innovagen and ToxinPred software,respectively.The potential peptides were docked with umami receptor T1R1/T1R3.Furthermore,taste properties of potential peptides were validated by electronic tongue.Docking results suggested that the three tetrapeptide EANK,EEAK,and EMQK could enter the binding pocket in the T1R1 cavity,wherein Arg151,Asp147,Gln52,and Arg277 may play key roles in the production of umami taste.Electronic tongue results showed that the umami value of EANK,EEAK,and EMQK were stronger than monosodium glutamate.This work provides a new insight for the screening of umami peptides in O.mykiss.

Identification and molecular mechanism of angiotensin-converting enzyme inhibitory peptides from Larimichthys crocea titin

This study aimed to identify novel ACEI peptides from Larimichthys crocea titin using in silico approaches and to clarify the molecular interaction mechanism.The hydrolyzed peptides of titin were compared with known ACEI peptides in the AHTPDB and BIOPEP-UWM database.Furthermore,peptides were evaluated for their solubility,ADMET properties,ΔG(kcal/mol)values,and in vitro ACEI activity.Molecular mechanism of ACE-peptide was performed by molecular interactions and binding orientation study.The results revealed that IC50 values of Trp-Ala-Arg(WAR)and Trp-Gln-Arg(WQR)were(31.2±0.8)and(231.33±0.02)mol/L,respectively.The docking interactions result suggested that ACE-WAR and ACEWQR complexes have same binding site,including the residues LYS511,TYR520,TYR523,HIS353,and HIS513.Molecular docking of two tripeptides WAR and WQR with ACE studies predicted their binding site and clarified the interaction between ACE and its inhibitors.The molecular docking data are consistent with the ACE inhibitory activity of the studied peptides.The results showed that Larimichthys crocea titin may be a valuable source for developing nutraceutical food.

Biological evaluation and interaction mechanism of beta-site APP cleaving enzyme 1 inhibitory pentapeptide from egg albumin

Inhibition of beta-site APP cleaving enzyme1(BACE1)is one of the most promising therapeutic approaches for Alzheimer’s disease.To find natural products for the treatment of Alzheimer’s disease,absorption,distribution,metabolism,excretion and toxicity(ADMET)properties and in vitro BACE1 inhibitory activity of the peptides isolated from egg albumin were evaluated.Then,molecular docking and molecular dynamics simulation were used to explain the molecular mechanism of the interactions between BACE1 and peptides.The IC50 value of peptide KLPGF,with satisfactory ADMET properties,against BACE1 was(8.30±0.56)mmol/L.Molecular docking revealed that KLPGF contacted with the residues of BACE1’s active sites through twelve hydrogen bonds interactions,two hydrophobic interactions,one electrostatic interaction,and two Pi-cation interactions.The 5 ns molecular dynamics simulations confirmed that the structure of KLPGF with BACE1 was stable.Peptide KLPGF contacted the residues Lys321,Asp228,and Asn233 with stable hydrogen bonds.KLPGF may be a potential anti-BACE1 candidate.

造纸废水处理的PLC控制系统的应用

采用厌氧IC反应器处理废水能够有效节约运行成本和时间,但相对而言,对自动控制的要求也较高。基于造纸工业废水及其工艺流程的特点,从系统结构、软硬件配置和实现功能几方面阐述了自动控制系统的应用。并着重论证了IC反应器进水的自动控制,指出自动控制系统应是紧密结合工艺,集控制功能、管理功能和数据采集处理功能于一体的系统。这样才能保证预期的工艺效果。

Microfluidic systems for axonal growth and regeneration research

Damage to the adult mammalian central nervous system （CNS） often results in persistent neurological deficits with limited recovery of functions. The past decade has seen in- creasing research efforts in neural regeneration research with the ultimate goal of achieving functional recovery. Many studies have focused on prevention of further neural damage and restoration of functional connections that are com- promised after iniurY or pathological damage.

DWARF and SMALL SEED1,a Novel Allele of OsDWARF,Controls Rice Plant Architecture,Seed Size,and Chlorophyll Biosynthesis

Plant architecture is a vital agronomic trait to control yield in rice(Oryza sativa L.).A dwarf and small seed 1(dss1)mutant were obtained from the ethyl methanesulfonate(EMS)mutagenized progeny of a Guizhou glutinous landrace cultivar,Lipingzabianhe.The dss1 mutant displayed phenotypes similar to those of brassinosteroid(BR)deficient mutants,such as dwarfing,dark green and rugose erect leaves,small seeds,and loner neck internode panicles with primary branching.In our previous study,the underlying DSS1 gene was isolated,a novel allele of OsDWARF(OsBR6ox)that encodes a cytochrome P450 protein involved in the BR biosynthetic pathway by MutMap technology.In this work,we confirmed that a Thr335Ile amino acid substitution residing in DSS1/OsDWARF was responsible for the dwarf,panicle architecture,and small seed phenotypes in the dss1 mutants by genetic transformation experiments.The overexpression of OsDWARF in the dss1 mutant background could not only recover dss1 to the normal plant height and panicle architecture but also rescued normal leaf angles,seed size,and leaf color.Thus,the specific mutation in DSS1/OsDWARF influenced plant architecture,seed size,and chlorophyll biosynthesis.

Identification and Characterization of a Novel Yellow Leaf Mutant yl1 in Rice

Leaf-color mutants play an important role in the study of chlorophyll metabolism,chloroplast development,and photosynthesis system.In this study,the yellow leaf 1(yl1)rice mutant was identified from the ethyl methane sulfonate-treated mutant progeny of Lailong,a glutinous japonica rice landrace cultivated in Guizhou Province,China.Results showed that yl1 exhibited yellow leaves with decreased chlorophyll content throughout the growth period.Chloroplast development in the yl1 mutant was disrupted,and the grana lamellae was loosely packed and disordered.RNA sequencing and real-time quantitative polymerase chain reaction(qRT-PCR)analysis revealed that the chlorophyll synthesis-related genes OsCHLH,OsCHLM,OsCHLG,PORB,and YGL8,as well as the chloroplast development-related genes FtsZ,OsRpoTp,and RbcL,were down-regulated in the yl1 mutant.Genetic analysis revealed that the yellow leaf phenotype of yl1 was controlled by recessive nuclear gene.By employing the MutMap method,the mutation responsible for the phenotype was mapped to a 6.17 Mb region between 17.34 and 23.51 Mb on chromosome 3.Two non-synonymous single-nucleotide polymorphisms(SNPs)located in the gene locus LOC_Os03g31210 and LOC_Os03g36760 were detected in this region.The two SNPs were further confirmed by PCR and Sanger sequencing.The expression patterns of the two candidate genes indicated that LOC_Os03g36760 showed greater potential for functional verification.Subcellular protein localization revealed that the encoded product of LOC_Os03g36760 was localized in the nucleus,cytoplasm,and plasma membrane.These results will be useful for further characterization and cloning of the yl1 gene,and for research on the molecular mechanisms controlling biogenesis and chloroplast biochemical processes.

A stable Co(Ⅱ)-based metal-organic framework with dual-functional pyrazolate-carboxylate ligand:Construction and CO_(2) selective adsorption and fixation

By taking the functional adva ntages of both pyrazolate and carboxylate ligands,a unique dual-functional pyrazolate-carboxylate ligand acid,4-(3,6-di(pyrazol-4-yl)-9-carbazol-9-yl)benzoic acid(H3PCBA) was designed and synthesized.Using it,a new Co(Ⅱ)-based metal-organic framework(MOF),Co_(3)(PCBA)_(2)(H_(2)O)_(2)(BUT-75) has been constructed.It revealed a(3,6)-connected net based on the 6-connected linear trinuclear metal node,and showed good chemical stability in a wide pH range from 3 to 12 at room temperature,as well as in boiling water.Due to the presence of rich exposed Co(Ⅱ) sites in pores,BUT-75 presented high selective CO_(2) adsorption capacity over N2 at 298 K.Simultaneously,it demonstrated fine catalytic performance for the cycloaddition of CO_(2) with epoxides into cyclic carbonates under ambient conditions.This work has not only enriched the MOF community through integrating diverse functio nalities into one ligand but also contributed a versatile platform for CO_(2) fixation,thereby pushing MOF chemistry forward by stability enhancement and application expansion.

A Highly Selective and Sensitive Fluorescent Probe Recognition for Co^2＋ in Aqueous Media Based on 8-Hydroxyquinolin-2- carbaldehyde-2-pyridylformylhydrazone Derivative

A new （8-hydroxyquinolin-2-yl）methylene picolinohydrazide derivative （L） has been successfully synthesized and characterized. The probe L displays high selectivity to Co^2＋ in CH3CN/HEPES （1：1, V/ V, 10 mmol.L^-1 pH=7.4） with a fluorescence ＂ON-OFF＂ response. The Co^2＋ ion recognition event possesses some distinct features including rapid response, high selectivity and sensitivity, good anti-interference ability and being applicable within a wide pH range. Based on job＇s plot and ESI-MS studies, the 1 ： 1 binding mode was proposed. The binding constant of L and Co2＋ is 1.63×10^8 L·mol^-1 and the detection limit is 1.15μmol·L^-1. Natural water samples experiments revealed that probe L can be potentially applied to the detection of Co^2＋ in real environment.

regeneration of thermally deactivated commercial V-W-Ti catalysts

An effective method for the regeneration of thermally deactivated commercial monolith SCR catalysts was investigated. Two types of regenerated solutions, namely NH4C1 （l mol/L） and dilute H2SO4 （0.5 tool/L）, were employed to treat the used catalyst. The effects of temperature and the regeneration process on the structural and textural properties of the catalysts were determined by X-ray diffraction, scanning electron microscopy, N2 adsorption/desorption, elemental analysis and Fourier transform infrared spectroscopy. The results suggest that the anatase phase of the used catalyst is maintained after exposure to high temperatures. Some of the catalytic activity was restored after regeneration. The catalyst regenerated by aqueous NH4C1 had a higher activity than that of the catalyst treated by dilute H2SO4. The main reason is that the NH3 generated from the decomposition of NH4C1 at high temperatures can be adsorbed onto the catalyst which promotes the reaction. The aggregated V205 were partially re-dispersed during the regeneration process, and the intrinsic oxidation of ammonia with high concentrations of O2 is a factorthat suppresses the catalytic activity.

An anionic In(Ⅲ)-based metal-organic framework with Lewis basic sites for the selective adsorption and separation of organic cationic dyes

In this paper, a new anionic metal-organic framework, [In(PBPTTBA)][(CH_3)_2 NH_2](BUT-29) has been synthesized through the reaction of tetratopic acid ligand with double Lewis pyridine sites, 4,4',4'',4'''-(4,4'-(1,4-phenylene)bis(pyridine-6,4,2-triyl)) tetrabenzoic acid(H_4 PBPTTBA) and In(NO_3)_2·5H_2O and fully characterized by single-crystal X-ray diffraction(SXRD), powder X-ray diffraction(PXRD),thermogravimetric analysis(TGA), infrared spectroscopy(IR), and elemental analysis(EA). BUT-29 can be used as an efficient adsorbent for the selective removal of organic cationic dyes in N,N0-dimethylformamide(DMF) solution. The adsorption capacities of BUT-29 toward methylene blue and crystal violet at 298 K can reach 1119 mg/g and 832 mg/g, respectively. Moreover, the adsorbed dyes can be released in the DMF solution of LiNO_3 gradually.

A new insight into the unique magneto-optical effect of layered perovskite(C_(6)H_(5)C_(2)H_(3)FNH_(3))_(2)MnCl_(4)

Magnet-optical materials embracing coupled magnetic and photoluminescent properties in single phase are promising in microelectronics and optoelectronic devices.However,the current research mainly focuses on traditional inorganic materials,and there are few reports on molecule materials.Recently,we synthesized an organic–inorganic hybrid complex((C_(6)H_(5)C_(2)H_(3)FNH_(3))_(2)MnCl_(4)(1)with perovskite structure.Physical measurements show that 1 not only behaves as an antiferromagnet with spin canting but also exhibits unusual fluorescent properties.Importantly,under the magnetic field at different temperatures,the luminous intensity of 1 changed,and a red-shift occurred with obviously optical hysteresis.These phenomena directly prove the existence of magneto-optical coupling in 1.More interestingly,the optical hysteresis can be observed in both low and high field,which is unprecedented in other molecular materials.Even in traditional inorganic materials,it can only be observed in strong field.This special function provides the possibility for the application of low energy consumption optoelectronic devices.

为了未来而记录历史——冯骥才的田野写作与文化思考研究

一切依据田野,来自田野,忠实田野……冯骥才先生的写作既是一位作家的创造性文学创作,也是一位"文化人"抢救文化遗产的真实的创造性的思想记录、历程纪实——其意义何在?冯骥才的写作有一个重要的特点:他借助虚构的文学的创意写作方式记录文化、又以非虚构的文化的写作记录非物质文化遗产,形成了典型的"双非写作"特色——冯骥才先生将他的写作追求和成果称之为"田野写作学"。

通向先祖心灵的探索之门——庆阳祁秀梅剪纸艺术及其传承

祁秀梅被联合国教科文组织认定为"人类口头和非物质文化遗产代表性传承人",是我国当代民间原生态剪纸最有影响的艺术家。乔晓光认为:"祁秀梅创造了剪纸的传奇,也创造了一个乡村女人的传奇。"并将她列入"中国民间剪纸天才传承者"之一。

庆阳民间绘画钩沉

“庆阳民间画”作为黄土高原黄河中游原陕甘宁边区的文化品牌,正在聚力复苏,其历程值得回顾。庆阳民间艺术在全国有重要地位,其民间画曾经在全国有过重要影响。在现代农民画的发展中,甘肃庆阳市曾是全国典范,起过领头作用。却未能像户县农民画那样保持历史荣誉,形成地方文化品牌。改革开放以来也没有能够像安塞农民画、宜君农民画那样,发展成为全国民间画典范。近年,随着非物质文化遗产保护和文化产业的发展,庆阳市民间文艺家协会致力于庆阳民间画复兴事业,带领庆阳民间艺术家展开新的实践,民间画得以新生,融入现代城市生活。同时甘肃农民书画研究会在庆阳建立培训基地,开展人才培训学术研讨和展览评比活动,获得令人欣喜的成绩。

经天营地说窑洞--论陇东窑洞民居抢救保护的价值

本文将陇东窑洞和窑洞营造技艺的保护传承放在文化生态与自然生态整体保护的思想理论下审视,结合农业文化遗产的保护,认为陇东窑洞村落文化属于华夏文明的特定区域,为先周文化传承典型的农耕文明成果,是丝绸之路东路最具特色的人文地理资源。通过从信仰、礼仪、审美、安居等层面论述分析了窑洞的历史意义及其价值,认为通过保护与提升,让窑洞建筑发挥其艺术之美、生活之美、文化之美,让人们充分认识和尊重窑洞作为人类文化艺术的价值和地位,并意识到窑洞保护不是孤立的“营造技艺”的保护,它是传统村落保护,是文化遗产与非物质文化遗产、农业文化遗产和自然文化遗产并重的多重保护共同体。在整体的生态保护前提下,选择典型的窑洞村落形成连锁旅游的乡村振兴文旅体系是陇东窑洞保护的可行方式。

歌谣、谚语与江南农事——浙江民间文献里的季候文化钩沉

民间艺术有着丰富的农耕文化与季候节气文化内涵,反映季候规律的二十四节气和相关的故事、歌谣、谚语,同时也是民间艺术创作的重要题材。本文以浙江民间文献为切入点,从季候文化的角度,以二十四节气作为线索,将其间的歌谣和谚语予以梳理。这些歌谣与谚语与当地的农业生产制度、农民的实际生产与生活,有着具体的对应性,能够反映当时我国农业生产制度的形成的季候、物候方面的因素,加深我们对农耕文化的深层认识。