Comprehensive Evaluation of Distributed PV Grid-Connected Based on Combined Weighting Weights and TOPSIS-RSR Method

To effectively quantify the impact of distributed photovoltaic(PV)access on the distribution network,this paper proposes a comprehensive evaluation method of distributed PV grid connection combining subjective and objective combination of assignment and technique for order preference by similarity to an ideal solution(TOPSIS)—rank sum ratio(RSR)(TOPSIS-RSR)method.Based on the traditional distribution network evaluation system,a comprehensive evaluation system has been constructed.It fully considers the new development requirements of distributed PV access on the environmental friendliness and absorptive capacity of the distribution grid and comprehensively reflects the impact of distributed PV grid connection.The analytic hierarchy process(AHP)was used to determine the subjective weights of the primary indicators,and the Spearman consistency test was combined to determine the weights of the secondary indicators based on three objective assignment methods.The subjective and objective combination weights of each assessment indicator were calculated through the principle of minimum entropy.Calculate the distance between the indicators to be evaluated and the positive and negative ideal solutions,the relative closeness ranking,and qualitative binning by TOPSIS-RSR method to obtain the comprehensive evaluation results of different scenarios.By setting up different PV grid-connected scenarios and utilizing the IEEE33 node simulation algorithm,the correctness and effectiveness of the proposed subject-object combination assignment and integrated assessment method are verified.

高灵敏乙型肝炎病毒表面抗原和DNA检测的临床应用价值

慢性乙型肝炎抗病毒治疗适应证逐渐扩大,对各种程度治愈(部分治愈、功能性治愈、完全治愈)的追求不断提高,使得临床上对于提高乙型肝炎病毒表面抗原(HBsAg)和乙型肝炎病毒(HBV)DNA检测灵敏度的需求越来越迫切。该文从目前国内外高灵敏HBsAg和HBV DNA检测试剂的上市情况,及其在HBV感染诊断、抗病毒治疗适应证和治疗方案的选择、疗效预测和停药指导、疗效判定和疾病结局的预测等方面的应用,指出高灵敏检测技术在慢性HBV感染治疗的全程管理中具有重要作用。

Multiomics analysis provides new insights into the regulatory mechanism of carotenoid biosynthesis in yellow peach peel

Carotenoids,as natural tetraterpenes,play a pivotal role in the yellow coloration of peaches and contribute to human dietary health.Despite a relatively clear understanding of the carotenoid biosynthesis pathway,the regulatory mechanism of miRNAs involved in carotenoid synthesis in yellow peaches remain poorly elucidated.This study investigated a total of 14 carotenoids and 40 xanthophyll lipids,including six differentially accumulated carotenoids:violaxanthin,neoxanthin,lutein,zeaxanthin,cryptoxanthin,and(E/Z)-phytoene.An integrated analysis of RNA-seq,miRNA-seq and degradome sequencing revealed that miRNAs could modulate structural genes such as PSY2,CRTISO,ZDS1,CHYB,VDE,ZEP,NCED1,NCED3 and the transcription factors NAC,ARF,WRKY,MYB,and bZIP,thereby participating in carotenoid biosynthesis and metabolism.The authenticity of miRNAs and target gene was corroborated through quantitative real-time PCR.Moreover,through weighted gene coexpression network analysis and a phylogenetic evolutionary study,coexpressed genes and MYB transcription factors potentially implicated in carotenoid synthesis were identified.The results of transient expression experiments indicated that mdm-miR858 inhibited the expression of PpMYB9 through targeted cleavage.Building upon these findings,a regulatory network governing miRNA-mediated carotenoid synthesis was proposed.In summary,this study comprehensively identified miRNAs engaged in carotenoid biosynthesis and their putative target genes,thus enhancing the understanding of carotenoid accumulation and regulatory mechanism in yellow peach peel and expanding the gene regulatory network of carotenoid synthesis.

eALT-F:A New Non-Invasive Staging Method to Identify Medium to High-Risk Patients with HCC from Ultra-High HBV Viral Load Population—China,2010-2023

Background:The objective of this study was to examine the clinical characteristics of individuals with ultra-high hepatitis B virus(HBV)viral load and develop a novel staging method for chronic hepatitis B(CHB)that can more effectively identify patients with medium to high hepatocellular carcinoma(HCC)risk.Methods:A total of 2,118 patients with HBV DNA>1×10^(7) IU/mL who visited Peking University People’s Hospital between January 2010 and March 2023 were enrolled retrospectively.Clinical data from the first visit were obtained and analyzed.The traditional phases and new‘eALT-F’stages were compared to evaluate the risk of HCC.Results:In the overall patients,more than onethird of the patients were under 30 years old.Additionally,a small proportion of older people(>60 years)also had ultra-high HBV viral load(4.3%).9.1%and 6.7%of individuals with ultra-high HBV viral load showed FIB-4>3.25 and aMAP≥50,respectively.In the traditional stages of CHB,which are based on HBeAg and alanine aminotransferase(ALT)[the upper limit of normal(ULN)ALT level at 40 IU/L for both men and women],regardless of phase,a certain proportion of patients were at risk of developing HCC(4.1%,6.4%,25.0%,and 20.3%).However,in the new‘eALT-F’stages,which are based on HBeAg,ALT(the ULN of ALT level at 30 IU/L for men and 19 IU/L for women),and/or FIB-4 levels(>1.45),aMAP≥50 was only observed in chronic hepatitis patients with positive or negative HBeAg(6.4%and 22.1%,respectively).Conclusions:The‘eALT-F’staging method,based on HBeAg,ALT(males:the ULN of ALT was 30 IU/L,females:19 IU/L)and/or FIB-4 levels,was more effective in identifying medium to high-risk patients with HCC from patients with ultra-high HBV viral load than the traditional staging methods.