Total flavonoids of Astragalus membranaceus protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity in mice by inhibiting ferroptosis through SLC7A11/GPX-4 signaling pathway

Parkinson’s disease(PD)is a common neurodegenerative disorder with no cure.Astragalus membranaceus is used in Chinese culture as a food supplement to boost immunity.The present study aimed to explore the neuroprotective effects of total flavonoids extracted from A.membranaceus(TFA)and their protective mechanisms.TFA offered neuroprotection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)in the mouse model of Parkinsonism,by improving behavior performance in the gait analysis and pole test,and inhibiting the decline of tyrosine hydroxylase(TH)positive neurons and TH protein expression in substantia nigra of mice.TFA also prevented 1-methyl-4-phenylpyridinium(MPP+)induced neurotoxicity in SHSY5Y cells,by increasing GSH and GSH/GSSG ratio,and reducing reactive oxygen species.In addition,the neuroprotective effects of TFA were associated with its ability to restore MPTP/MPP+induced downregulation of SLC7A11 and glutathione peroxidase 4(GPX-4).In conclusion,we demonstrated that TFA exerted significant neuroprotection against MPTP/MPP+induced neurodegeneration by inhibiting ferroptosis through the regulation of SLC7A11/GPX-4 axis,suggesting the use of TFA as a possible food supplement in the prevention of PD.

虾青素对河蟹生长性能、色泽及其品质的影响

开展了虾青素对河蟹生长性能、色泽及其品质的影响试验。分别设置雌、雄2个试验组和雌雄2个对照组。试验组投喂含虾青素的饲料,对照组投喂普通商品饲料,6—8月连续投喂10 d/月。结果表明,在饲料中添加虾青素,可显著提高雄蟹肝胰腺的亮度、红色与黄色饱和度以及背甲的黄色保护度(P<0.05);显著提高雌蟹背甲的亮度(P<0.05);显著提高雄蟹的鲜味、甜味、肉香味、细嫩度、咀嚼性、黏附性和整体喜好性,减少氨味(P<0.05);显著提高雌雄河蟹的过氧化氢酶与谷胱甘肽过氧化物酶含量(P<0.05),降低丙二醛含量(P<0.05);降低雄蟹的超氧化物歧化酶含量(P<0.05),提高雌蟹的总抗氧化能力含量(P<0.05)。

Electromagnetic responses on microstructures of duplex stainless steels based on 3D cellular and electromagnetic sensor finite element models

Microstructures determine mechanical properties of steels,but in actual steel product process it is difficult to accurately control the microstructure to meet the requirements.General microstructure characterization methods are time consuming and results are not rep-resentative for overall quality level as only a fraction of steel sample was selected to be examined.In this paper,a macro and micro coupled 3D model was developed for nondestructively characterization of steel microstructures.For electromagnetic signals analysis,the relative permeability value computed by the micro cellular model can be used in the macro electromagnetic sensor model.The effects of different microstructure components on the relative permeability of duplex stainless steel(grain size,phase fraction,and phase distribu-tion)were discussed.The output inductance of an electromagnetic sensor was determined by relative permeability values and can be val-idated experimentally.The findings indicate that the inductance value of an electromagnetic sensor at low frequency can distinguish dif-ferent microstructures.This method can be applied to real-time on-line characterize steel microstructures in process of steel rolling.

Helicobacter pylori infection may result in poor gastric cleanliness in magnetically controlled capsule gastroscopy examination: A singlecenter retrospective study

Objective:Magnetically controlled capsule gastroscopy(MCCG)is an effective method for screening gastric diseases;however,its performance may be affected by gastric cleanliness.We aimed to explore the correlation between Helicobacter pylori infection and the degree of gastric cleanliness in the MCCG.Methods:This retrospective study enrolled 297 participants from October 2020 to April 2024 at Sir Run Run Shaw Hospital,Zhejiang University School of Medicine.Participant characteristics,MCCG examination results,and(13)C-urea breath test(C13-UBT)results were collected.The gastric cleanliness in MCCG examinations was assessed using a gastric cleanliness score.Binary logistic regression was used to analyze the relationships among participant characteristics,H.pylori infection,and gastric cleanliness.Chi-square tests and Fisher's exact tests were used to analyze the relationships among gastric lesions,H.pylori infection,and gastric cleanliness.Results:Among the participants,24.2%had H.pylori infection,and 17.5%had poor gastric cleanliness.Hypertension(odds ratio[OR]:2.63;95%confidence interval[CI]:1.36e5.09;p?0.004)was associated with a greater likelihood of H.pylori infection.H.pylori infection(OR:3.76;95%CI:1.99e7.09;p<0.001)was an independent risk factor for poor gastric cleanliness in the MCCG.A significant disparity was noted in the prevalence of focal erosions(p<0.001),gastric ulcers(p?0.001),and positive gastric lesions(p?0.027)between the 2 groups with and without H.pylori infection.The proportion of positive gastric lesions was not significantly different between the good gastric cleanliness group and the poor gastric cleanliness group(25.7%vs.21.2%;p?0.490).Conclusion:The findings of this study revealed that H.pylori infection was associated with hypertension.H.pylori infection may lead to poor gastric cleanliness.Institutions are advised to perform C13-UBT before MCCG,and participants should be informed of the risk of poor gastric cleanliness if the results are positive.The decision to perform H.pylori eradication before MCCG should take into account patient willingness and the benefit-to-risk ratio.

Effects of Thickness and Anisotropic Strain on Polarization Switching Properties of Sub-10nm Epitaxial Hf_(0.5)Zr_(0.5)O_(2)Thin Films

Doped HfO_(2)-based ferroelectric(FE)films are emerging as leading contenders for next-generation FE nonvolatile memories due to their excellent compatibility with complementary metal oxide semiconductor processes and robust ferroelectricity at nanoscale dimensions.Despite the considerable attention paid to the FE properties of HfO_(2)-based films in recent years,enhancing their polarization switching speed remains a critical research challenge.We demonstrate the strong ferroelectricity of sub-10nm Hf_(0.5)Zr_(0.5)O_(2)(HZO)thin films and show that the polarization switching speed of these thin films can be significantly affected by HZO thickness and anisotropically strained La_(0.67)Sr_(0.33)MO_(3)-buffered layer.Our observations indicate that the HZO thin film thickness and anisotropically strained La_(0.67)Sr_(0.33)MO_(3)layer influence the nucleation of reverse domains by altering the phase composition of the HZO thin film,thereby reducing the polarization switching time.Although the increase in HZO thickness and anisotropic compressive strain hinder the formation of the FE phase,they can enable faster switching.Our findings suggest that FE HZO ultrathin films with polar orthorhombic structures have broad application prospects in microelectronic devices.These insights into novel methods for increasing polarization switching speed are poised to advance the development of high-performance FE devices.

“枫桥经验”在医疗纠纷调解领域的实践与发展——基于甘肃第三方医疗纠纷人民调解委员会的调研

“依靠群众就地化解矛盾”是“概桥经验”的人民调解的精髓,研究、实践如何把人民调解精髓引入医疗纠纷调解领域,不仅对抓好医疗纠纷风险防范和医疗纠纷人民调解、服务保障和改善民生有着重大意义,也对推动人民调解工作、实现基层预防化解矛盾与基层社会治理联动,提高国家治理体系与治理能力现代化具有极为重要的现实意义。本文基于甘肃第三方医疗纠纷人民调解委员会的实践调硏,详细分析了医疗纠纷产生的原因,对第三方人民调解的内涵、范围、特点和执行要点进行了深入阐释,证明在医疗纠纷调解领域坚持发展“枫桥经验”具备理论、实践双重价值。

Modified laparoscopic Sugarbaker repair of parastomal hernia with a three-point anchoring technique

AIMTo retrospectively evaluate the safety and feasibility of a new modified laparoscopic Sugarbaker repair in patients with parastomal hernias.METHODSA retrospective study was performed to analyze eight patients who underwent parastomal hernia repair between June 2016 and January 2018. All of these patients received modified laparoscopic Sugarbakerhernia repair treatment. This modifed technique included an innovative three-point anchoring and complete su-turing technique to fix the mesh. All procedures were performed by a skilled hernia surgeon. Demographic data and perioperative outcomes were collected to eva-luate the safety and effcacy of this modifed technique.RESULTSOf these eight patients, two had concomitant incisional hernias. All the hernias were repaired by the modifed laparoscopic Sugarbaker technique with no conversion to laparotomy. Three patients had in-situ reconstruc-tion of intestinal stoma. The median mesh size was 300 cm2, and the mean operative time was 205.6 min. The mean postoperative hospitalization time was 10.4 d, with a median pain score of 1 （visual analog scale method） at postoperative day 1. Two patientsdeveloped postoperative complications. One patient had a pocket of effusion surrounding the biologic mesh, and one patient experienced an infection around the reconstructed stoma. Both patients recovered after conservative management. There was no recurrence during the follow-up period （6-22 mo, average 13 mo）.CONCLUSIONThe modifed laparoscopic Sugarbaker repair could fx the mesh reliably with mild postoperative pain and a low recurrence rate. The technique is safe and feasible for parastomal hernias.

Quantitative prediction process and evaluation method for seafloor polymetallic sulfide resources

Seafloor polymetallic sulfide resources exhibit significant development potential. In 2011, China received the exploration rights for 10,000 km2 of a polymetallic sulfides area in the Southwest Indian Ocean; China will be permitted to retain only 25% of the area in 2021. However, an exploration of seafioor hydrothermal sulfide deposits in China remains in the initial stage. According to the quantitative prediction theory and the exploration status of seafloor sulfides, this paper systematically proposes a quantitative prediction evaluation process of oceanic polymetallic sulfide resources and divides it into three stages： prediction in a large area, prediction in the prospecting region, and the verification and evaluation of targets. The first two stages of the prediction process have been employed in seafloor sulfides prospecting of the Chinese contract area. The results of stage one suggest that the Chinese contract area is located in the high posterior probability area, which indicates good prospecting potential area in the Indian Ocean. In stage two, the Chinese contract area of 48^-52~E has the highest posterior probability value, which can be selected as the reserved region for additional exploration. In stage three, the method of numerical simulation is employed to reproduce the ore-forming process of sulfides to verify the accuracy of the reserved targets obtained from the three-stage prediction. By narrowing the exploration area and gradually improving the exploration accuracy, the prediction will provide a basis for the exploration and exploitation of seafloor polymetallic sulfide resources.

Electrophoretically deposited binder-free 3-D carbon/sulfur nanocomposite cathode for high-performance Li–S batteries

In the present study,the electrophoretic deposition method was successfully applied as a binder-free and scalable approach to deposit carbonaceous nanomaterials on carbon fiber paper(CFP)for cathode applications in Li-S batteries.The microstructural studies of the EPD-CNT film using scanning electron microscopy(SEM)revealed the formation of a crack-free and porous layer of CNTs being uniformly distributed on the CFP surface.The EPD:CFP/CNT/S cathode delivered a capacity around 2.2 times higher than that obtained in the absence of the EPD-CNT film(CFP/S cell)after 50 cycles and a capacity of935 mAh g^-1 after 100 cycles at 0.1 C.The EPD method was then employed to fabricate layer-by-layer structures where the EPD-CNT film was decorated with carbon black particles.The initial capacity as well as the reversible capacity after 100 cycles was further increased by the EPD:CFP/CNT/KB/S layer-by-layer structure to 1473 and 1033 mAh g^-1,respectively,indicating effective suppression of the shuttle effect.In addition,the rate performance of CFP/S was improved by depositing the EPD-CNT and EPD-CNT/carbon black architectures on CFP surface,and even further enhanced through the co-deposition of CNT and Pt nanoparticles by EPD,delivering a specific capacity of around 730 mAh g^-1 at 1 C.Finally,the cathodes fabricated by EPD were observed to outperform those made by the conventional casting method in terms of cycling performance,internal resistance,and polarization.This difference was ascribed to the non-uniform microstructure of the Cast-CNT film,which resulted in poor interfacial connection between the CNT agglomerates,hindering uniform sulfide/sulfur deposition during cycling.The obtained results suggested that the binder-free C/S nanocomposite cathode made by EPD is key to further enhance the specific capacity and energy density of Li-S batteries.

论新发展理念的传统文化意蕴

新发展理念是习近平新时代中国特色社会主义思想的重要组成部分,也是党关于发展理论的重大创新。它的提出既源于以习近平同志为核心的党中央对当前国内外发展大势的深入把握,也根植于优秀传统文化,具有深厚的传统文化意蕴。其中,创新发展理念是对传统“日新”思想深入而全面的传承与弘扬;协调发展理念是对传统“贵和”思想的继承与超越;绿色发展理念是对传统“天人合一”思想的科学发展与升华;开放发展理念是对传统“重交”观念的弘扬与丰富;共享发展理念是对传统“大同”理想的现代阐释与创新发展。

Optimal design of vibration absorber using minimax criterion with simplified constraints

In this paper, a minimax design of damped dynamic vibration absorber for a damped primary system is investigated to minimize the vibration magnitude peaks. Moreover, to reduce the sensitivity of the primary system response to variations of the forcing frequency for a two- degree-of-freedom system, the primary system should have two equal resonance magnitude peaks. To meet this re- quirement, a set of simplified constraint equations includ- ing distribution characteristics of the resonant frequencies of the primary system is established for the minimax objective function. The modified constraint equations have less un- known variables than those by other authors, which not only simplifies the computation but also improves the accuracy of the optimal values. The advantage of the proposed method is illustrated through numerical simulations.

明清乡村生态资源管理规约及其现代价值

明清时期,乡村面临着日益严峻的生态资源压力。乡村社会素有诉诸乡规民约开展自治的历史传统,乡村生态资源管理规约也由此生成。乡村生态资源管理规约主张协力同办、共育共治资源,公平严切、和谐共享资源,以时节取、严行保护资源,追求人与人团结共治,人与自然和谐共生。在实践路径上,乡村生态资源管理规约以一定地域的乡村民众尤其是民众组织或群体为主体,以内部精英为主导与核心,以成员的共同认可、自觉遵守为基础,以官方意志和力量的介入为重要时代特征。当前,乡村生态资源管理规约可以为乡村生态文明制度体系,提供地方性传统知识和规则惯习的补充与支撑。

全球贫困的治理:人道主义还是正义?——从安格斯·迪顿对贫困援助的反思谈起

针对全球贫困治理问题,存在着两种较有影响力的理论,即人道主义理论和全球正义理论。虽然这两种理论立场不同,但都主张对贫困人口进行援助,而且两者可以互相补充,具有同等的道德重要性。安格斯·迪顿对“援助错觉”和“两难困境”的解释,揭示了贫困治理中人道主义和正义之间存在冲突。迪顿认为,对贫困人口进行人道主义援助的目的是让受助者受益,但这种援助却往往成为阻碍穷人改善生活的一块绊脚石,因而是一件不正义的事情。贫困治理中人道主义和正义的冲突,也是我国贫困治理中面临的一个重要问题,这要求我们对贫困者的界定应从泛泛而论转向具体剖析,了解贫困者的特殊性表现,从而实施更有针对性的援助项目。

Minimally invasive method to treat a rare wrist injury with simultaneous fractures of the scaphoid and hook of hamate: A case report and literature review

Concomitant fractures of the hook of hamate and scaphoid are rare injuries to the wrist. Whenever these fractures cannot be effectively managed, nonunion or osteonecrosis is encountered. Subsequent treatment is thus much more challenging for a hand surgeon or orthopedist. Minimally invasive percutaneous internal fixation is an optimal treatment with the potential to minimize injury and reduce fractures. However, the control of iatrogenic injuries, including possible damage to the adjacent vital tissue, is a challenge that needs to be addressed. Herein,we describe the case of a 26-year-old man who presented with fractures to the scaphoid and hook of hamate in his left wrist. Minimally invasive treatment-with closed reduction and percutaneous fixation of the scaphoid, wrist arthroscopy, and closed reduction and percutaneous fixation of the hook of hamate-was performed at our institution. The Mayo score of the wrist, visual analog scale(VAS) for pain, grip strength, pinch strength, and wrist motion in radial/ulnar and flexion/extension deviation were recorded. Primary healing was achieved in both fractures. At the final follow-up, the Mayo score of the wrist was 100(excellent), the VAS score was 0(no pain),and the grip and pinch strength of his injured hand were 90.9% and 83.3%, respectively, compared with the contralateral hand(grip strength: left, 50 kg;right, 55 kg. Pinch strength: left, 20 kg;right, 24 kg). The radialulnar, flexion-extension, and forearm pronation-supination directions were 30°, 140°, and 90°, respectively.Minimally invasive closed reduction with percutaneous internal fixation is an optimal technique with satisfactory outcomes for simultaneous fractures of the hook of hamate and scaphoid. Provided in this paper are details of the technique and technical suggestions for performing the procedure.

Zinc finger-inspired peptide-metal-phenolic nanointerface enhances bone-implant integration under bacterial infection microenvironment through immune modulation and osteogenesis promotion

Orthopedic and dental implantations under bacterial infection microenvironment face significant challenges in achieving high-quality bone-implant integration. Designing implant coatings that incorporate both immune defense and anti-inflammation is difficult in conventional single-functional coatings. We introduce a multifunctional nanointerface using a zinc finger-inspired peptide-metal-phenolic nanocoating, designed to enhance implant osseointegration under such conditions. Abaloparatide (ABL), a second-generation anabolic drug for treating osteoporosis, can be integrated into the design of a zinc-phenolic network constructed on the implant surface (ABL@ZnTA). Importantly, the phenolic-coordinated Zn2+ ions in ABL@ZnTA can act as zinc finger motif to co-stabilize the configuration of ABL through multiple molecular interactions, enabling high bioactivity, high loading capacity (1.36 times), and long-term release (>7 days) of ABL. Our results showed that ABL@ZnTA can modulate macrophage polarization from the pro-inflammatory M1 towards the anti-inflammatory M2 phenotype, promoting immune osteogenesis with increased OCN, ALP, and SOD 1 expression. Furthermore, the ABL@ZnTA significantly reduces inflammatory fibrous tissue encapsulation and enhances the long-term stability of the implants, indicated by enhanced binding strength (6 times) and functional connectivity (1.5−3 times) in the rat bone defect model infected by S. aureus. Overall, our research offers a nano-enabled synergistic strategy that balances infection defense and osteogenesis promotion in orthopedic and dental implantations.

A pilot study on Paxlovid therapy for hemodialysis patients with severe acute respiratory syndrome coronavirus 2 infections

We aimed to investigate the safety and efficacy of nirmatrelvir/ritonavir(Paxlovid)therapy for hemodialysis-dependent patients with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Thirteen hemodialysis patients infected with the Omicron variant of SARS-CoV-2 from April 3 to May 30,2022,were recruited.Laboratory parameters and chest CT(computed tomography)imaging were analyzed.The treatment group included six patients who received 150 mg/100 mg of Paxlovid orally once daily for 5 days,whereas the control group included seven patients who received basic treatment.No serious adverse reactions or safety events were recorded.Four control patients progressed to moderate disease,and none in the treatment group showed progression of chest CT findings(P<0.05).Paxlovid therapy tended toward early viral clearance and low viral load on Day 8.Moreover,83.3%of the patients in the treatment group and 57.1%of the patients in the control group turned negative within 22 days.In the Paxlovid treatment group,we found significantly increased levels of lymphocytes(P=0.03)and eosinophils(P=0.02)and decreased levels of D-dimer on Day 8 compared with those on Day 1.Paxlovid therapy showed a potential therapeutic effect with good tolerance in hemodialysis patients.The optimal dose and effectiveness evaluation must be further investigated in a largeer cohort.

Bacterial community network complexity and role of stochasticity decrease during primary succession

In microbial ecology,there is limited understanding of the mechanisms governing patterns in community structure across space and time.Here,we studied the changes of bacterial co-occurrence network structure over four primary successional soils after glacier retreat,including a sand dune system and three glacier foreland series,varying in timescale from centuries to millennia.We found that in all series,network structure was most complex in the earliest stages of succession,and became simpler over time.Richness and abundance of keystone species and network stability also declined over time.It appears that with less productive,nutrient poor and physiologically extreme conditions of early succession,closer interactions among bacterial species are ecologically selected for.These may take the form of consortia(with positive interactions)or stronger niche exclusion(with negative interactions).Additionally,we quantified the relative roles of different structuring processes on bacterial community using a bin-based null model analysis(iCAMP).With each successional series,community composition was initially governed by stochasticity,but as succession proceeded there was a progressive increase in deterministic selection over time,correlated with decreasing pH.Overall,our results show a consistency among the four series in long-term processes of community succession,with more integrated networks and greater stochasticity in early stages.

棘突间动态稳定装置Coflex治疗腰椎管狭窄症的10年以上随访观察

目的评估棘突间动态稳定系统Coflex治疗腰椎管狭窄症10年以上的临床疗效及对关节突退变的影响。方法收集我院自2009年1月至2013年6月采用减压联合Coflex棘突间动态稳定手术治疗的22例腰椎管狭窄症患者,其中男10例,女12例;年龄40~76岁,平均(59.6±12.6)岁。所有患者均完善影像学和临床评估。影像学评估包括:手术节段及其邻近节段腰椎活动度(range of motion,ROM),椎间隙高度(intervertebral space height,ISH),椎间孔高度(intervertebral foraminal height,IFH)和关节突退变情况及邻近节段退变(adjacent segment degeneration,ASD)发生率;临床评估包括:患者术前及末次随访时疼痛视觉模拟评分(visual analogue scale,VAS),Owestry功能障碍指数(Owestry disability index,ODI)及日本骨科协会(Japanese Orthopaedic Association,JOA)评分,同时记录手术相关并发症。结果共收集22例腰椎管狭窄症患者,随访时间121~175个月,平均156个月。临床疗效方面,术前和末次随访时腰痛VAS、腿痛VAS、ODI、JOA评分分别为7.36±0.67、7.09±0.70、50.45±6.46、12.82±1.33和1.82±0.98、1.64±0.81、9.09±5.39、22.82±2.93,末次随访时所有评分均较术前改善,与术前相比差异有统计学意义(P<0.01)。影像学评估方面,无论手术节段还是相邻上下节段,ISH及IFH术后与术前相比差异有统计学意义(P<0.05);末次随访时,ISH及IFH与术前相比差异无统计学意义(P>0.05)。末次随访时,手术节段ROM与术前相比差异有统计学意义(P<0.05);但对相邻上下节段,ROM与术前相比差异无统计学意义(P>0.05)。关节突退变方面,末次随访时,相邻上节段与术前相比差异有统计学意义(P<0.05),手术节段及相邻下节段与术前相比差异无统计学意义(P>0.05)。结论Coflex治疗腰椎管狭窄症10年以上临床疗效肯定。同时,Coflex能够有效维持ISH及IFH,保留了椎间活动度,此外,还能有效减缓手术节段关节突关节退变。

2008—2017年绍兴市青春期女性卵巢肿瘤133例临床资料分析

目的分析2008—2017年绍兴市青春期女性卵巢肿瘤临床资料,为青春期卵巢肿瘤的早期筛查提供医学建议。方法回顾性分析2008年1月—2017年12月绍兴市妇幼保健院收治的133例青春期卵巢肿瘤患者的发病情况、临床表现、辅助检查、病理特点及治疗情况。结果13岁后,卵巢肿瘤发病率逐渐升高,15岁后明显升高。初潮后1~5年共发病118例(88.72%)。月经异常和腹痛、腹胀为主要的首诊症状。卵巢巨大肿瘤(肿瘤最大径线>10 cm)的比例较高(30/148)。恶性肿瘤2例,均为无性细胞瘤,均行手术治疗+化疗;良性肿瘤125例,以成熟型畸胎瘤最多见(79例)。结论早期筛查有利于早期发现卵巢肿瘤并及时治疗,减少严重并发症的发生,保留生育功能,维护心理健康。

2084例宫颈不能明确意义的非典型鳞状上皮细胞改变患者的结果分析

目的了解绍兴地区宫颈液基细胞学(LBC)检查诊断为不能明确意义的非典型鳞状上皮细胞改变(ASC-US)患者的高危型人乳头瘤病毒(HR-HPV)感染、宫颈鳞状上皮内瘤变(CIN)和宫颈癌的患病情况.方法选取2015年11月至2017年11月在绍兴市妇幼保健院行LBC检查诊断为ASC-US并同时接受HR-HPV检测、阴道镜检查及组织病理学检查的2 084例患者为研究对象,对其结果进行回顾性分析.结果在2 084例ASC-US患者中,宫颈病理学检查阴性共1 651例,占79.22%,CINⅡ~Ⅲ及宫颈癌236例,占11.32%;HR-HPV感染者共1 334例,HPV感染率为64.01%.病理阴性者HR-HPV感染率为55.72%(920/1 651),而CIN及宫颈癌患者高达95.61%(414/433),两者相比差异有统计学意义(x2=236.922,P<0.01).<45岁者中,≥CINⅡ者占16.12%(137/850),≥45岁者中,≥CINⅡ者占18.60%(90/484),两组差异有统计学意义(x2=10.401,P<0.05).HR-HPV感染率随CIN级别增高呈上升趋势(x2趋势=208.499,P<0.01).结论 ASC-US患者中HR-HPV感染率和宫颈病理确诊阴性者占比均较高.HR-HPV感染与CIN及宫颈癌存在相关性.