Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis

Osteoarthritis(OA)is a common degenerative disease worldwide and new therapeutics that target inflammation and the crosstalk between immunocytes and chondrocytes are being developed to prevent and treat OA.These attempts involve repolarizing pro-inflammatory M1 macrophages into the anti-inflammatory M2 phenotype in synovium.In this study,we found that phosphoglycerate mutase 5(PGAM5)significantly increased in macrophages in OA synovium compared to controls based on histology of human samples and single-cell RNA sequencing results of mice models.To address the role of PGAM5 in macrophages in OA,we found conditional knockout of PGAM5 in macrophages greatly alleviated OA symptoms and promoted anabolic metabolism of chondrocytes in vitro and in vivo.Mechanistically,we found that PGAM5 enhanced M1 polarization via AKT-mTOR/p38/ERK pathways,whereas inhibited M2 polarization via STAT6-PPARγpathway in murine bone marrow-derived macrophages.Furthermore,we found that PGAM5 directly dephosphorylated Dishevelled Segment Polarity Protein 2(DVL2)which resulted in the inhibition ofβ-catenin and repolarization of M2 macrophages into M1 macrophages.Conditional knockout of both PGAM5 andβ-catenin in macrophages significantly exacerbated osteoarthritis compared to PGAM5-deficient mice.Motivated by these findings,we successfully designed mannose modified fluoropolymers combined with siPGAM5 to inhibit PGAM5 specifically in synovial macrophages via intra-articular injection,which possessed desired targeting abilities of synovial macrophages and greatly attenuated murine osteoarthritis.Collectively,these findings defined a key role for PGAM5 in orchestrating macrophage polarization and provides insights into novel macrophage-targeted strategy for treating OA.

Chaotic CS Encryption:An Efficient Image Encryption Algorithm Based on Chebyshev Chaotic System and Compressive Sensing

Images are the most important carrier of human information. Moreover, how to safely transmit digital imagesthrough public channels has become an urgent problem. In this paper, we propose a novel image encryptionalgorithm, called chaotic compressive sensing (CS) encryption (CCSE), which can not only improve the efficiencyof image transmission but also introduce the high security of the chaotic system. Specifically, the proposed CCSEcan fully leverage the advantages of the Chebyshev chaotic system and CS, enabling it to withstand various attacks,such as differential attacks, and exhibit robustness. First, we use a sparse trans-form to sparse the plaintext imageand then use theArnold transformto perturb the image pixels. After that,we elaborate aChebyshev Toeplitz chaoticsensing matrix for CCSE. By using this Toeplitz matrix, the perturbed image is compressed and sampled to reducethe transmission bandwidth and the amount of data. Finally, a bilateral diffusion operator and a chaotic encryptionoperator are used to perturb and expand the image pixels to change the pixel position and value of the compressedimage, and ultimately obtain an encrypted image. Experimental results show that our method can be resistant tovarious attacks, such as the statistical attack and noise attack, and can outperform its current competitors.

Literature classification and its applications in condensed matter physics and materials science by natural language processing

The exponential growth of literature is constraining researchers’access to comprehensive information in related fields.While natural language processing(NLP)may offer an effective solution to literature classification,it remains hindered by the lack of labelled dataset.In this article,we introduce a novel method for generating literature classification models through semi-supervised learning,which can generate labelled dataset iteratively with limited human input.We apply this method to train NLP models for classifying literatures related to several research directions,i.e.,battery,superconductor,topological material,and artificial intelligence(AI)in materials science.The trained NLP‘battery’model applied on a larger dataset different from the training and testing dataset can achieve F1 score of 0.738,which indicates the accuracy and reliability of this scheme.Furthermore,our approach demonstrates that even with insufficient data,the not-well-trained model in the first few cycles can identify the relationships among different research fields and facilitate the discovery and understanding of interdisciplinary directions.

Burden of gastrointestinal cancers among working-age population over past thirty years in China

BACKGROUND Although gastrointestinal(GI)cancers have been becoming a great public health concern in China,there is currently a lack of comprehensive literature on the overall burden and changing trends of GI cancers in the working-age population.AIM To assess the burden of GI cancers and to examine the overall,age-and genderspecific trends among the working-age population in China from 1990 to 2019.METHODS Data were extracted from the Global Burden of Disease Study 2019.The burden of GI cancers was indicated by incidence,mortality,disability-adjusted life-years(DALYs),age-standardized incidence rate(ASIR),age-standardized mortality rate,and age-standardized DALYs rate.Trends in the burden of GI cancers from 1990 to 2019 were examined using annual percent change and average annual percent change with Joinpoint regression models.RESULTS For overall GI cancers,a declining trend was observed in the ASIR,age-standardized mortality rate,and agestandardized DALYs rate,with reductions of 0.74%,2.23%,and 2.22%,respectively,from 1999 to 2019 in the Chinese working-age population.However,an increasing trend was observed in the ASIR for overall GI cancers from 2016-2019.The number of either incident cases,mortality cases,and DALYs was higher for colon/rectum cancer and liver cancer in younger participants but lower for esophageal,gallbladder,biliary tract,pancreatic,and stomach cancer among older subjects.Moreover,sex disparity in the GI cancers burden was also examined over 30 years.CONCLUSION The total burden of GI cancers remained heavy among the working-age population in China,although declining trends were observed from 1999 to 2019.Disparities in the GI cancers burden existed between sexes,age groups,and cancer types.Population-based precision prevention strategies are needed to tackle GI cancers among working-age individuals,considering the age,sex,and cancer type disparities in China.

Simulation-Based Investigation of Crack Formation in Forged MB15 Magnesium Alloy

In the process of riveting an MB15 forging die,cracks were discovered emerging along the longitudinal direction and near the riveting hole.Through fracture analysis,microscopic observation,energy spectrum analysis,metallographic examination,and hardness test,the properties and causes of the cracks are discussed.The results indicate that the cracking type is intergranular brittle cracking,occurring during the forging stage.Furthermore,the recrystallization at the crack site is found to be incomplete,which is attributed to the low deformation temperature.

胜坨油田无短板长寿井举升技术研究与应用

胜利采油厂胜坨油田油井举升系统矛盾突出,躺井率一度高达8.9%,严重制约了油田的经济开发。胜坨油田强化技术攻关,优化资金投入,倾力打造无短板长寿井,延长油井生产周期。通过打造无短板长寿井,油田躺井率由2010年的8.9%下降至2017年的2.1%,效果显著。使油田开发工作进入良性循环。

体育公共服务公众评价实证研究

采用文献资料法、问卷调查法、数理统计法等方法,对体育公共服务期望值与满意度评价进行实证研究。研究结果表明:不同性别、年龄、职业对体育公共服务的期望值与满意程度存在差异。建议:政府需要重新界定行政部门角色、明确政府职能、建立健全相应法律法规等,从而建设服务型政府。

基于反卷积算法的超声图像优化方法

超声成像技术因其无侵入、低成本、快速、可便携化的特性,成为医学成像领域的一大研究热点.然而,受限于声波的传播特性、成像算法的弊端以及硬件发展,超声图像存在成像深度小、大量伪影、分辨率低等问题.针对超声图像中的目标混叠和分辨率低下问题,提出了一种基于信号处理的超声图像优化方法,通过反卷积算法对超声探头采集到的原始信号进行处理,再将处理后的信号按照延时求和成像算法重建为图像.提出的方法可以减轻信号间的混叠,最终减轻图像中的混叠,令图像中原本难以辨认的微小结构和细节信息得以展现.通过仿真和实验证明,经过处理后的信号所重建的图像质量优于原始信号所重建的图像,验证了提出的信号处理方法的有效性.

脑梗死患者Cysc、Hcy水平与疾病严重程度及预后的关系

目的探讨脑梗死患者胱抑素C(Cysc)、同型半胱氨酸(Hcy)水平与疾病严重程度以及预后的关系。方法选取脑梗死患者110例,运用神经功能缺损程度评分(NIHSS)评定患者脑梗死严重程度,根据评分结果将其分为3组,其中轻型缺损(A组)55例,中型缺损(B组)41例,重型缺损(C组)14例;预后效果采用改良Rankin量表进行统计,根据评分结果分为良好组59例、不良组51例。于病发第2、14天时采用免疫比浊法测定所有患者的Cysc、Hcy水平,同时对Cysc、Hcy水平与疾病严重程度及预后的关系进行相关性分析。结果脑梗死患者病发第14天Cysc、Hcy水平低于病发第2天水平;C组患者Cysc、Hcy水平明显高于A、B组(P<0.05),3组两两比较差异有统计学意义(P<0.05);良好组Cysc、Hcy水平明显低于不良组(P<0.05);Cysc、Hcy水平与疾病严重程度呈正相关(P<0.05),与预后情况呈负相关(P<0.05)。结论 Cysc、Hcy水平与疾病严重程度及预后具有一定相关性,在患者病情进展预测方面具有一定的参考价值。

2型糖尿病患者抑郁与血同型半胱氨酸、血脂及颈动脉粥样硬化的关系研究

目的探讨2型糖尿病(T2DM)患者抑郁与血同型半胱氨酸(Hcy)、血脂及颈动脉粥样硬化(CAS)之间的关系。方法选取2015年11月-2016年11月华北理工大学附属唐山市工人医院住院治疗的146例T2DM患者,根据汉密顿抑郁量表及抑郁症诊断标准将其分为T2DM非抑郁组105例和T2DM合并抑郁组41例,分别测定两组空腹血糖(FBG)、Hcy、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A1(Apo A1)、载脂蛋白B1(Apo B1)、脂蛋白a(LP-a)水平,应用彩色多普勒超声仪行颈动脉内膜-中层厚度检查,记录有无颈动脉硬化发生。结果 (1)T2DM合并抑郁组颈动脉硬化检出率75.6%(31例)较T2DM非抑郁组55.2%(58例)高。(2)与T2DM非抑郁组比较,T2DM合并抑郁组Hcy、TG均有升高,而LDL-C、LP-a低于T2DM非抑郁组。两组患者FBG、TC、Apo A1、Apo B1差异无统计学意义(P>0.05)。(3)Logistic多因素回归分析示Hcy和TG为T2DM患者合并抑郁的独立相关因素。(4)Spearman相关性分析示T2DM患者Hcy和TG与HAMD量表评分呈正相关。结论医务人员应重视T2DM患者血脂和同型半胱氨酸水平,评估颈动脉硬化情况,积极预防糖尿病患者抑郁的发生,促进其心理健康。

阻塞性睡眠呼吸暂停综合征对视野及视神经纤维层厚度的影响

目的:探讨阻塞性睡眠呼吸暂停综合征(OSAS)对患者视野及视网膜神经纤维层(RNFL)厚度的影响。方法:OSAS患者74例74眼及72名健康体检者(72眼)作为对照组被纳入研究。采用Humphrey自动视野计检测视野敏感度(VFS),采用频域光学相干断层扫描仪(OCT)测量视盘周围RNFL厚度。VFS及视盘周围RNFL被划分为上方、下方、颞侧及鼻侧区域。按照多导睡眠图(PSG)监测获得的呼吸暂停低通气指数(AHI)对OSAS病情进行分级。将29例轻度、25例中度及20例重度OSAS患者上方、下方、颞侧和鼻侧区域的VFS及RNFL厚度分别与对照组进行统计学比较。结果:OSAS组与对照组上方、下方、颞侧和鼻侧区域的VFS均无差异(P>0.05)。OSAS组尤其是重度OSAS患者视盘鼻侧RNFL厚度较对照组降低(P=0.047)。Pearson相关性分析显示鼻侧RNFL厚度与OSAS患者病情呈负相关(r=-0.9998,P=0.0138)。结论:重度OSAS可导致鼻侧RNFL厚度减少,RNFL厚度可作为评估OSAS病情严重程度的指标之一。

衰老过程中行为和认知功能退化的调控机制研究

随着人类预期寿命延长,人口老龄化问题越来越严重。过去几十年关于衰老的研究使人们对长寿的生物学机理有了一定的认识,然而延长寿命应以保持老年个体健康的行为和认知功能为前提,近期研究显示延长寿命不一定延缓衰老过程中的行为和认知功能退化。衰老相关行为退化的调控机制目前知道的还很少,如何实现老年人口健康的衰老是现代社会极具挑战也是迫切需要解决的问题。衰老过程伴随着明显的认知等行为功能的退化,过去的研究对这些功能的退化进行了比较详细的描述,包括情节记忆、工作记忆、信息处理速度等认知功能的衰退,运动能力降低,节律紊乱等。随着神经科学与技术的发展,越来越多的研究集中到大脑的结构和功能随衰老的改变。本文在简单描述衰老过程中行为功能退化现象的基础上,主要对大脑结构和网络连接、神经元形态和功能、大脑基因表达以及一些保守的生物学信号通路等方面在衰老过程中的改变的研究进展展开综述性介绍,重点关注这些变化与行为和认知功能退化之间的联系。目前大部分的研究结果还只建立了这些变化与行为和认知功能退化的相关关系,因果关系的确立还有待进一步的研究。相信更多对衰老过程中行为和认知功能退化的调控机制的研究将对改善老年人的生活质量有极大帮助,同时对寻找预防神经退行性疾病发生的方法也有指示作用。

A Nano-Micro Engineering Nanofiber for Electromagnetic Absorber,Green Shielding and Sensor

It is extremely unattainable for a material to simultaneously obtain efficient electromagnetic(EM)absorption and green shielding performance,which has not been reported due to the competition between conduction loss and reflection.Herein,by tailoring the internal structure through nano-micro engineering,a NiCo2O4 nanofiber with integrated EM absorbing and green shielding as well as strain sensing functions is obtained.With the improvement of charge transport capability of the nanofiber,the performance can be converted from EM absorption to shielding,or even coexist.Particularly,as the conductivity rising,the reflection loss declines from −52.72 to −10.5 dB,while the EM interference shielding effectiveness increases to 13.4 dB,suggesting the coexistence of the two EM functions.Furthermore,based on the high EM absorption,a strain sensor is designed through the resonance coupling of the patterned NiCo2O4 structure.These strategies for tuning EM performance and constructing devices can be extended to other EM functional materials to promote the development of electromagnetic driven devices.

Developing MXenes from Wireless Communication to Electromagnetic Attenuation

There is an urgent global need for wireless communication utilizing materials that can provide simultaneous flexibility and high conductivity.Avoiding the harmful effects of electromagnetic(EM)radiation from wireless communication is a persistent research hot spot.Two-dimensional(2D)materials are the preferred choice as wireless communication and EM attenuation materials as they are lightweight with high aspect ratios and possess distinguished electronic properties.MXenes,as a novel family of 2D materials,have shown excellent properties in various fields,owing to their excellent electrical conductivity,mechanical stability,high flexibility,and ease of processability.To date,research on the utility of MXenes for wireless communication has been actively pursued.Moreover,MXenes have become the leading materials for EM attenuation.Herein,we systematically review the recent advances in MXene-based materials with different structural designs for wireless communication,electromagnetic interference(EMI)shielding,and EM wave absorption.The relationship governing the structural design and the effectiveness for wireless communication,EMI shielding,and EM wave absorption is clearly revealed.Furthermore,our review mainly focuses on future challenges and guidelines for designing MXene-based materials for industrial application and foundational research.

Validation of the six-and-twelve criteria among patients with hepatocellular carcinoma and performance score 1 receiving transarterial chemoembolization

BACKGROUND Transarterial chemoembolization(TACE)is recommended for patients with intermediate hepatocellular carcinoma(HCC)according to treatment guidelines.However,a large number of patients with advanced HCC also receive TACE in clinical practice,especially for those with liver-confined HCC and Eastern Cooperative Oncology Group score(ECOG)1.In view of previous studies,such patients have different prognoses from advanced HCC patients with macrovascular invasion or extrahepatic spread;therefore,patients with ECOG 1 alone might be classified into the intermediate stage and benefit from TACE treatment,but a study particularly focusing on such patients and exploring the effectiveness of TACE therapy is lacking.AIM To investigate treatment outcomes of TACE in HCC patients with ECOG 1 alone and propose a specific prognostic model.METHODS Patients from 24 Chinese tertiary hospitals were selected in this nationwide multicenter observational study from January 2010 to May 2016.Overall survival(OS)was estimated using Kaplan–Meier curves and compared by the log-rank test.Multivariate Cox regression was used to develop the potential prognostic models.The discriminatory ability of the models was compared and validated in various patient subgroups.The individual survival prediction for six-and-twelve(6&12)criteria,defined as the algebraic sum of tumor size(cm)and tumor number,was illustrated by contour plot of 3-year survival probability and nomogram.RESULTS A total of 792 eligible patients were included.During follow-up,median OS reached 18.9 mo[95%confidence interval(CI):16.9-21.0].Three independent multivariate analyses demonstrated that tumor size,tumor number,α-fetoprotein level,albumin–bilirubin grade and total bilirubin were prognostic factors of OS(P<0.05).The previously proposed 6&12 criteria was comparable or even better than currently proposed with the highest predictive ability.In addition,the 6&12 criteria was correlated with OS in various subgroups of patients.The patients were stratified into three strata with score≤6,>6 but≤12,and>12 with different median OS of 39.8 mo(95%CI:23.9-55.7),21.1 mo(95%CI:18.4-23.8)and 9.8 mo(95%CI:8.3-11.3),respectively(P<0.001).CONCLUSION TACE is effective for advanced HCC patients with ECOG 1 alone,and the 6&12 criteria may help with clinical decision-making.

Validation and evaluation of clinical prediction systems for first and repeated transarterial chemoembolization in unresectable hepatocellular carcinoma: A Chinese multicenter retrospective study

BACKGROUND The treatment outcome of transarterial chemoembolization(TACE)in unresectable hepatocellular carcinoma(HCC)varies greatly due to the clinical heterogeneity of the patients.Therefore,several prognostic systems have been proposed for risk stratification and candidate identification for first TACE and repeated TACE(re-TACE).AIM To investigate the correlations between prognostic systems and radiological response,compare the predictive abilities,and integrate them in sequence for outcome prediction.METHODS This nationwide multicenter retrospective cohort consisted of 1107 unresectable HCC patients in 15 Chinese tertiary hospitals from January 2010 to May 2016.The Hepatoma Arterial-embolization Prognostic(HAP)score system and its modified versions(mHAP,mHAP2 and mHAP3),as well as the six-and-twelve criteria were compared in terms of their correlations with radiological response and overall survival(OS)prediction for first TACE.The same analyses were conducted in 912 patients receiving re-TACE to evaluate the ART(assessment for re-treatment with TACE)and ABCR(alpha-fetoprotein,Barcelona Clinic Liver Cancer,Child-Pugh and Response)systems for post re-TACE survival(PRTS).RESULTS All the prognostic systems were correlated with radiological response achieved by first TACE,and the six-and-twelve criteria exhibited the highest correlation(Spearman R=0.39,P=0.026)and consistency(Kappa=0.14,P=0.019),with optimal performance by area under the receiver operating characteristic curve of 0.71[95%confidence interval(CI):0.68-0.74].With regard to the prediction of OS,the mHAP3 system identified patients with a favorable outcome with the highest concordance(C)-index of 0.60(95%CI:0.57-0.62)and the best area under the receiver operating characteristic curve at any time point during follow-up;whereas,PRTS was well-predicted by the ABCR system with a C-index of 0.61(95%CI:0.59-0.63),rather than ART.Finally,combining the mHAP3 and ABCR systems identified candidates suitable for TACE with an improved median PRTS of 36.6 mo,compared with non-candidates with a median PRTS of 20.0 mo(logrank test P<0.001).CONCLUSION Radiological response to TACE is closely associated with tumor burden,but superior prognostic prediction could be achieved with the combination of mHAP3 and ABCR in patients with unresectable liver-confined HCC.

Tunable superconductivity in parent cuprate Pr_2CuO_(4±δ) thin films

We studied the role of oxygen in Pr2 CuO_(4±δ) thin films fabricated by the polymer assisted deposition method. The magnetoresistance and Hall resistivity of Pr2 CuO_(4±δ) samples were systematically investigated. It was found that with decreasing oxygen content, the low-temperature Hall coefficient(RH) and magnetoresistance changed from negative to positive, similar to those with the increase of Ce-doped concentration in R_(2-x)Ce_x CuO_4 (R = La, Nd, Pr, Sm, Eu). In addition, we observed that the dependence of the superconducting critical temperature Tc with RH for the Pr_(2-x) Ce_x CuO_4 perfectly overlapped with that of Pr_2 CuO_(4±δ) . These findings point to the fact that the doped electrons induced by the oxygen removal are responsible for the superconductivity of the T-phase parent compounds.

含立构复合晶体的聚己内酯形状记忆弹性体的制备及性能

以聚己内酯(PCL)、左旋丙交酯(LLA)、右旋丙交酯(DLA)为原料,以辛酸亚锡(Sn(Oct)2)为催化剂,合成了不同LA含量的P(LLA-b-CL)和P(DLA-b-CL)嵌段共聚物。用凝胶渗透色谱、核磁共振等研究了嵌段共聚物的组成与结构。将2种嵌段共聚物按一定比例溶液共混,并通过流延法得到PCL基形状记忆弹性体薄膜,研究了其结晶性能、晶体形态、力学性能及形状记忆性能。结果表明,随LA添加量增大,弹性体薄膜中的PLLA与PDLA形成的立构复合(SC)晶体结晶度增大;同时,PLA非晶区含量提高,70℃拉伸时PLA链段产生滑移,形状回复率降低。因此,弹性体中SC晶体的结晶度越高,形状回复率越高,形状回复率最佳可达93.54%。

VEGF-C/VEGFR-3/iNOS Signaling in Osteosarcoma MG63 Cells Mediates Stimulatory Effects on Human Umbilical Vein Endothelial Cell Proliferation

Objectives To assess the effects of vascular endothelial growth factor-C(VEGF-C)/vascular endothelial growth factor receptor-3(VEGFR-3)signaling on nitric oxide(NO)production and inducible nitric oxide synthase(iNOS)expression in human osteosarcoma MG63 cells and the subsequent impact on the proliferation of human umbilical vein endothelial cells(HUVECs).Methods MG63 cells were treated with VEGF-C alone(VEGF-C group),VEGF-C+iNOS inhibitor aminoguanidine(AG;AG group),and VEGF-C+VEGFR-3 inhibitor MAZ51(MAZ51 group);untreated MG63 cells were used as controls.NO production was evaluated by a colorimetric method involving nitrate reductase.Meanwhile,mRNA and protein levels of iNOS were examined by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.To explore the effect of VEGF-C/VEGFR-3/iNOS signaling of MG63 cells on proliferation of HUVECs,we set up six groups:HUVECs,HUVECs+MG63,HUVECs+VEGF-C,HUVECs+MG63+VEGF-C,HUVECs+MG63+VEGF-C+AG,and HUVECs+MG63+VEGF-C+MAZ51 groups.The proliferation of HUVEC cells was assessed by Cell Counting Kit-8(CCK-8),5-ethynyl-2'-deoxyuridine(EdU)incorporation assay,and proliferating cell nuclear antigen(PCNA)expression quantitation.Results VEGF-C treatment enhanced iNOS expression at both gene and protein levels(mRNA:LSD-t=4.152,P<0.01;protein:LSD-t=3.486,P<0.01)and increased NO release of MG63 cells(LSD-t=3.774,P<0.01);treatment with either AG or MAZ51 decreased these effects(mRNA:LSD-t=9.183,P<0.001;LSD-t=8.639,P<0.001;protein:LSD-t=5.170,P<0.001;LSD-t=7.25S,P<0.001;NO production:LSD-t=10.326,P<0.001;LSD-t=l0.540,P<0.001).Interestingly,co-incubation of HUVECs with MG63 cells and/or VEGF-C significantly promoted HUVEC proliferation(EdU:LSD-t=5.374,P<0.001;LSD-t=2.984,P<0.05;LSD-t=8.526,P<0.001;PCNA:LSD-t=9.267,P<0.001;LSD-t=5.515,P<0.001;LSD-t=14.873,P<0.001).The proliferation effects of HUVEC induced by MG63 cells and VEGF-C attenuated by the treatment of AG(EdU:LSD-t=10.770,P<0.001;PCNA:LSD-t=19.94O,P<0.001)or MAZ51(EdU:LSD-t=6.950,P<0.001;PCNA:LSD-t=14.001,P<0.001).Conclusion In human osteosarcoma MG63 cells,activation of VEGFR-3 by VEGF-C promotes iNOS expression and NO production,which subsequendy induces HUVEC proliferation.

Optimization of large-area YBa_(2)Cu_(3)O_(7-δ)thin films by pulsed laser deposition for planar microwave devices

This paper presents high quality YBa_(2)Cu_(3)O_(7-δ)(YBCO)thin films on LaAlO_(3)substrate for microwave devices prepared by pulsed laser deposition(PLD).The double-sided YBCO films cover a large area and have been optimized for key parameters relevant to microwave device applications,such as surface morphology and surface resistance(R_(s)).This was achieved by improving the target quality and increasing the oxygen pressure during deposition,respectively.To evaluate the suitability of the YBCO films for microwave devices,a pair of microwave filters based on microstrip fabricated on films from this work and a commercial company were compared.The results show that the YBCO films in this work could completely meet the requirements for microwave devices.