Effect of Sonic hedgehog gene-modified bone marrow mesenchymal stem cells on graft-induced retinal gliosis and retinal ganglion cells survival in diabetic mice

AIM:To investigate the effects of Sonic hedgehog(Shh)gene-modified bone marrow mesenchymal stem cells(MSCs)on graft-induced retinal gliosis and retinal ganglion cells(RGCs)survival in diabetic mice.METHODS:Bone marrow-derived MSCs were genetically modified with the Shh gene to generate a stably transfected cell line of Shh-modified MSCs(MSC-Shh).Intravitreal injections of MSC-Shh and green fluorescent protein-modified MSCs(MSC-Gfp;control)were administered in diabetic mice.After 4wk,the effects of MSC-Shh on retinal gliosis were evaluated using fundus photography,and markers of gliosis were examined by immunofluorescence and Western blotting.The neurotrophic factors expression and RGCs survival in the host retina were evaluated using Western blotting and immunofluorescence.The mechanisms underlying the effects of MSC-Shh was investigated.RESULTS:A significant reduction of proliferative vitreoretinopathy(PVR)was observed after intravitreal injection of MSC-Shh compared to MSC-Gfp.Significant downregulation of glial fibrillary acidic protein(GFAP)was demonstrated in the host retina after MSC-Shh administration compared to MSC-Gfp.The extracellular signal-regulated kinase 1/2(ERK1/2),protein kinase B(AKT)and phosphatidylin-ositol-3-kinase(PI3K)pathways were significantly downregulated after MSC-Shh administration compared to MSC-Gfp.Brain-derived neurotrophic factor(BDNF)and ciliary neurotrophic factor(CNTF)levels were significantly increased in the host retina,and RGCs loss was significantly prevented after MSC-Shh administration.CONCLUSION:MSC-Shh administration reduces graft-induced reactive gliosis following intravitreal injection in diabetic mice.The ERK1/2,AKT and PI3K pathways are involved in this process.MSC-Shh also increases the levels of neurotrophic factors in the host retina and promoted RGCs survival in diabetic mice.

Simulation Analysis of Deformation Control for Magnetic Soft Medical Robots

Dear Editor,This letter presents a biocompatible cross-shaped magnetic soft robot and investigates its deformation mode control strategy through COMSOL modeling and simulation.Magnetic soft robots offer novel avenues for precise treatment within intricate regions of the human body.

A newly discovered major fault of the 1976 Tangshan earthquake in China

In the field investigations of the great 1976 Tangshan earthquake in China, several large subsidence areas with remarkable boundary fssures were reported as phenomena of the seismic event. Recently a re-interpretation of aerial photos taken immediately after the quake revealed that the areas were controlled by a giant fault of about 40 km in length; and subsidences were brought about by the movement of the fault during the shock, not being a consequence of soil liquefaction as suggested in some of the investigations. This discovery has been confirmed by the results of fine geophysical explorations carried out by the Jidong Oilfield Company, PetroChina. The newly discovered fault, named Fuzhuang-Xihe fault, is much longer than the one prevailingly accepted as the seismic fault of the Tangshan earthquake. Moreover, the former seems to be a normal fault, while the latter is regarded as a strike-slip one.

Thoughts on Present Situation of Development of Rural Animal Husbandry and Its Relationship with Construction of Ecological Agriculture and Revitalization of Rural Industry

After several rounds of phased ups and downs of development,rural animal husbandry finally presents a shrinking and depressed development situation under the comprehensive action of the failure of transformation and upgrading and the increasing production costs and breeding risks.Actively exploring and guiding the healthy and sound development of rural animal husbandry is not only an important measure to promote the construction of ecological agriculture,but also the main starting point to realize the revitalization of rural industry.This paper analyzes the present situation of the development of rural animal husbandry in recent years,the influence of animal husbandry on the construction of ecological agriculture and the revitalization of rural industry,and puts forward the corresponding measures,in order to promote the steady and efficient development of animal husbandry in the grand strategy of revitalizing rural industry.

Exploration and Discussion on Drug Management in the Operating Room

Operating room drug management work directly related to the safety of medication and the scientific nature of medication,the most important content of operating room drug management is to ensure the rational and safe use of drugs,to avoid drug abuse and misuse.This paper mainly explores the problems existing in the current operating room drug management process,points out the specific drug management model,and hopes to give full play to the role of drug management to ensure that drugs can be applied scientifically.

Construction Technology and Quality Control of Double Layer Continuous Paving of Cement Stabilized Macadam Base

The construction method of continuous paving of the upper and lower base of the water stable macadam base can improve the construction efficiency,overcome the quality problems of the traditional layered paving of the upper and lower base,such as untimely curing,slow construction,poor interlayer bonding,contraction joint and interlayer,so as to ensure the construction quality of the water stable macadam.It is also the promotion and application of new technology.

Phylogeny,molecular evolution,and dating of divergences in Lagerstroemia using plastome sequences

Lagerstroemia L.(Lythraceae)is a widely distributed genus of trees and shrubs native to tropical and subtropical environments from Southeast Asia to Australia,with numerous species highly valued as ornamentals.Although the plastomes of many species in this genus have been sequenced,the rates of functional gene evolution and their effect on phylogenetic analyses have not been thoroughly examined.We compared three plastome sequence matrices to elucidate how differences in these datasets affected phylogenetic analyses.Robust phylogenetic relationships for Lagerstroemia species were reconstructed based on different plastome sequence partitions and multiple phylogenetic methods.Identification of single-nucleotide variants within different genes also provides basic data on the patterns of functional gene evolution in Lagerstroemia and may provide insights into how those mutations affect protein structure and potentially drive divergence via cytonuclear incompatibility.These results as well as analyses of non-synonymous and synonymous mutations,indicate that heterotachic modes of evolution are present in functional plastome genes and should be accounted for in the analyses of molecular evolution.In addition,divergence events within the Lagerstroemia were dated for the first time.Several of the divergence estimates corresponded to well-known Earth history events,such as the reduction in global temperatures at the Eocene/Oligocene boundary.Our analyses conducted in Lagerstroemia here dissects the various patterns in the divergence of Lagerstroemia and may provide a useful guide to help plant breeders,as well as the necessity of using plastomic data and as possible as to combine evidence from morphological characteristics to investigate the complicated interspecies relationship and the evolutionary dynamics of species.

Analysis of Application Status and Recommendations for Shade-Tolerant Lawns and Ground Cover Plants in the West Lake Scenic Area

Taking 8 parks in Xihu Scenic Area as the survey objects, this study found that there are 77 kinds of shade-tolerant lawns and ground cover plants under the forest in good growth condition, and there are 3 types of application forms: dense forest, sparse forest and forest edge. In response to the existing problems, it is proposed that it is necessary to adhere to principles of ecology, adapt to local conditions and plant suitable varieties in accordance with local conditions, develop and use new varieties of wild shade-tolerant lawns and ground cover plants, and strengthen the promotion of the mixed planting model of shade-tolerant lawns and ground cover plants, so as to provide guidance for the better use of shade-tolerant lawns and ground cover under the forest in the West Lake Scenic Area in the future.

穴位注射与鼓室注射治疗特发性耳鸣的疗效比较

目的评估并分析穴位注射与鼓室注射治疗特发性耳鸣的疗效。方法选取2017年2月—2018年1月云南省昆明市第一人民医院耳鼻喉科收治的特发性耳鸣患者100例,将穴位注射给药患者作为穴位注射组,经鼓室注射给药患者作为鼓室注射组,应用耳鸣致残量表(THI)和视觉模拟量表(VAS)对患者手术前后耳鸣情况进行评估,比较两组患者术后耳鸣缓解情况。结果两组患者治疗后THI、VAS评分比较,差异有统计学意义(P <0.05),多数患者耳鸣有缓解,穴位注射组治疗有效率为82%,鼓室注射组为62%,差异有统计学意义(P<0.05)。结论穴位注射和鼓室注射都能有效治疗特发性耳鸣,但穴位注射给药方法有效率更高,且能更好地缓解患者耳鸣症状,疗效更好。

基于卷积神经网络的语种识别系统

从给定语音中提取有效语音段表示是语种识别的关键点。近年来深度学习在语种识别应用中有重要的进展,通过深度神经网络可以提取音素相关特征,并有效提升系统性能。基于深度学习的端对端语种识别系统也表现出其优异的识别性能。本文针对语种识别任务提出了基于卷积神经网络的端对端语种识别系统,利用神经网络强大的特征提取能力及区分性建模能力,提取具有语种区分性的基本单元,再通过池化层得到有效语音段表示,最后输入全连接层得到识别结果。实验表明,在NIST LRE 2009数据集上,相比于现阶段国际主流语种识别系统,提出的系统在30 s,10 s和3 s等语音段上错误率分别相对下降了1.35%,12.79%和29.84%,且平均错误代价在3种时长上均相对下降30%以上。

The evolution of microstructure and texture of AZ80 Mg alloy cup-shaped pieces processed by rotating backward extrusion

This study proposed an effective plastic deformation technique,rotating backward extrusion(RBE),for producing high performance AZ80 magnesium alloy cup-shaped pieces.The RBE process was carried on the Gleeble-3500 test machine at 653 K,and the conventional backward extrusion(CBE)was also conducted for comparison.A detailed microstructure analysis was performed using the optical microscopy(OM)and electron back-scatter diffraction(EBSD).The results shown that the equivalent strain and deformation uniformity of the cup pieces could be substantially increased by the RBE process compared with the CBE process.Furthermore,the RBE process could significantly improve the grain refining capacity and the proportion of dynamic recrystallization(DRX),of which the maximum reduction of grain size was 88.60%,and the maximum increase of DRX proportion was 55.30%in the cup bottom.The main deformation mechanism of the RBE process was the discontinuous DRX(DDRX),while the continuous DRX(CDRX)was also occurred in the cup transition.Compared with the CBE sample,the texture of the cup bottom was weakened for the RBE sample.The microhardness value of the RBE sample was higher than that of the CBE sample,which can be attributed to the grain refinement strengthening.

Longitudinal Measurement of Hemodynamic Changes within the Posterior Optic Nerve Head in Rodent Nonarteritic Anterior Ischemic Optic Neuropathy

ObjectiveTo assess the in vivo dynamic blood flow features of posterior optic nerve head (ONH) in rat model of nonarteritic anterior ischemic optic neuropathy (rNAION). MethodsrNAION was established with Rose Bengal and argon green laser in Sprague-Dawley rats. Fundus photography and fundus fluorescein angiography (FFA) were performed to assess the dynamic changes of optic disc in morphology in 90 days and in blood perfusion in 3 hours after the induction of disease. Histological examinations were performed to evaluate the success of modeling. Thedynamic blood flow kinetics of posterior ONH in rNAION were measured by Laser Doppler Flowmetry (LDF) on the day 3, 7, 14, 21, and 40 after the disease induction. One-way ANOVA, Student'st-test and Bonferroni adjustment were used for multiple comparisons of kinetic measurements of blood flow. ResultsOptic disc edema and subsequent resolution associated with the development of optic disc pallor were observed in rNAION. FFA showed that the optic disc was hypofluorescence in the early phase and hyperfluorescence in the late phase. Histological studies suggested edema and loosened tissues of ONH, loss of retinal ganglion cells (RGCs), optic nerve substance and gliosis. Compared to the naive rats, the blood flow kinetics of posterior ONH in rNAION significant reduced at each time point after modeling (F=175.06,P<0.0001). The reductions were specifically remarkable in 14 days after the disease induction (AllP<0.01). Conclusions Continuous blood perfusion reduction was found in rNAION, with significant alteration in 14 days after disease induction. Our results provided important information for understanding the hemodynamic changes in rNAION.

Self-reported Taste and Smell Disorders in Patients with COVID-19:Distinct Features in China

Last December 2019,a cluster of viral pneumonia cases identified as coronavirus disease 2019(COVID-19)was reported in Wuhan,China.We aimed to explore the frequencies of nasal symptoms in patients with COVID-19,including loss of smell and taste,as well as their presentation as the first symptom of the disease and their association with the severity of COVID-19.In this retrospective study,1206 laboratory-confirmed COVID-19 patients were included and followed up by telephone one month after discharged from Tongji Hospital,Wuhan.Demographic data,laboratory values,comorbidities,symptoms,and numerical rating scale scores(0–10)of nasal symptoms were extracted from the hospital medical records,and confirmed or reevaluated by the telephone follow-up.From patients(n=1172)completing follow-up,199(17%)subjects had severe COVID-19 and 342(29.2%)reported nasal symptoms.20.6%COVID-19 patients had loss of taste(median score=6),while 11.4%had loss of smell(median score=5).Loss of taste scores,but not loss of smell scores,were significantly increased in severe vs.nonsevere COVID-19 patients.Interleukin(IL)-6 and lactose dehydrogenase(LDH)serum levels were positively correlated with loss of taste scores.About 80%of COVID-19 patients recovered from smell and taste dysfunction in 2 weeks.In this cohort,only 1 out of 10 hospital admitted patients had loss of smell while 1 out of 5 reported loss of taste which was associated to severity of COVID-19.Most patients recovered smell and taste dysfunctions in 2 weeks.

Correlations between the optic nerve head morphology and ocular biometrics in highly myopic eyes

AIM： To analyze peripapillary atrophy β/γ zone （PPA-β/γ） and the optic disc ovality index, and to assess their associations with the axial length （AL）, refractive error, best corrected visual acuity （BCVA）, choroidal thickness （CT）, and age in highly myopic eyes. METHODS： This was a retrospective observational case series. The study included 667 patients consecutively examined for highly myopic eyes [spherical equivalent ≤-6.0 diopters （D） and AL≥26 mm] with or without myopic retinopathy. Each patient went through a comprehensive ophthalmological examination that included spectral domain optical coherence tomography （SD-OCT） of the macula, A-mode ultrasonography, and a cycloplegic refraction test. The ovality index and PPA-β/γ area were measured from optic disc photographs. RESULTS： A significant association was seen between PPA-β/γ area and the ovality index （P=0.000, r=-0.232）. The PPA-β/γ area increased significantly with a longer AL, older age, worse BCVA, higher refractive error, and thinner choroid （P〈0.01）. The oval disc was significantly correlated with a longer AL, older age, worse BCVA, higher refractive error, larger PPA-β/γ area, and thinner choroid （P〈0.01）. CONCLUSION： The PPA-β/γ zone and ovality index in highly myopic eyes show distinct associations with the AL, refractive error, BCVA, age, and CT.

博弈视角下不同规模企业的研发创新行为研究——基于中国啤酒企业的案例分析

针对企业规模与研发创新的驱动关系,本文从博弈论的视角出发,透过无博弈下企业创新、博弈下大企业创新与小企业剧烈创新的三种情景,深入剖析不同规模企业博弈下创新驱动的内在行为机理,最后以中国啤酒企业的数据进行实证分析。结果表明:在相互博弈基础上,拥有技术优势的大企业在一定程度上缺乏创新动力,会借助研发创新成果排除外在竞争者;小企业只有通过偶发的剧烈创新才有可能赶超大企业,得出了演化博弈视角下企业研发创新行为的经验启示。

Correlation between heart rate variability and pupillary reflex in healthy adult subjects under the influence of alcohol

This study explored the association between pupil area variation, heart rate variability, and fatigue, caused by alcohol consumption. Sixteen healthy young male volunteers were enrolled in this study for a series of structured physical examinations. Individual drinking volume was strictly assessed in accordance with the normalized experimental design. The motions of the subjects＇ pupils, as well as electrocardiograms were recorded by a computerized recording system, both before and after drinking alcohol. After alcohol intake, the pupil diameter, and both low frequency and standard deviation of all heartbeat interval indices of heart rate variability showed significant variations compared with the baseline. In addition, time- and frequency-domain indices of heart rate variability were negatively associated with pupil area. Thus the current study suggests that fatigue status may be determined by significant changes in pupil area and heart rate variability.

Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen

AIM:To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells(hR VECs),and explore its regulations by integrins and RhoA/ROCK1 signal pathway.METHODS:h RVECs were cultured in collagen and treated by opticin,and cell-based bioactivity assays of cell proliferation,migration,and adhesion were performed.The expression of integrinα2,integrinβ1,Rho A and ROCK1 were examined with real-time PCR and Western blotting.RESULTS:Collagen could promote cell viability of proliferation and migration(all P<0.05),and enhance the m RNA expression of integrinα2,integrinβ1,Rho A and ROCK1(all P<0.05).Opticin could inhibit proliferation and migration ability of hR VECs cultured in collagen,and reduce the mR NA expression of integrinα2,integrinβ1,RhoA and ROCK1(all P<0.05).CONCLUSION:Collagen and opticin can affect bioactivity of hR VECs,which may be regulated byα2-,β1-integrins and RhoA/ROCK1 signal pathway.

The 100 most influential articles in myopia:a bibliometric analysis

AIM:To identify and characterize the 100 most influential articles in the field of myopia over the last decades.METHODS:Articles on myopia published between January 1975 and March 2020 were searched through the Web of Science Core Collection database.Two independent authors reviewed and determined the 100 most cited articles.The characteristics of each eligible article were recorded,including authors,institutions,countries,journals,publication date,total citations(TCs),annual citations(ACs),research focus and article type.RESULTS:The top 100 most influential articles were published between 1983 and 2016,with 1999 as the most prolific year.The mean number of TCs was 288(range:193-537)and the mean number of ACs was 19(range:7-109).Treatment and epidemiology of myopia were the most important research focus.These articles were published in 21 journals led by Ophthalmology(29%)followed by Investigative Ophthalmology&Visual Science(23%).The number of ACs for articles published in the last ten years was significantly higher than that for the other most-cited articles(44 vs 16,Mann-Whitney U test P<0.01).There is no difference in the number of TCs between original articles and review articles,while the number of ACs for review articles was significantly higher than that for original articles(22 vs 17,Mann-Whitney U test P<0.05).CONCLUSION:This bibliometric analysis can provide us with concise information about the development trend of research in the field of myopia in the past few decades,and provide an important reference for researchers to guide future research.

Study on Torque Ripple of Wound-Field Doubly Salient Generator with No Load

Wound-field Doubly Salient Generator (WFDSG) has the outstanding advantages of simple structure, high reliability and controllable excitation. However, there exists obvious torque ripple in generator’s input torque, which has restricted its applications. Based on the concept of electromagnetic torque of WFDSG, the nonlinear formula under no load condition has been derived. Furthermore, it is proved to be reasonable theoretically in comparison with linear and approximate nonlinear formulas. Based on the 12/8 salient prototype, the correctness of nonlinear formula has been verified by comparing the results of virtual displacement method with the results of finite element analysis. At last, the simulation results have been validated by experiments.

“一带一路”背景下新疆哈萨克语专业课程设置问题与思考

基于"一带一路"大背景,以新疆师范高等专科学校哈萨克语专业为例,通过对现有哈萨克语专业课程体系的审视,提出把培养适应"一带一路"需求的掌握熟练的哈萨克语作为人才培养的核心目标,同时新疆的哈萨克语专业人才培养应向具备国际化人才的特点聚焦。