Piezoelectricity in native bones has been well recognized as the key factor in bone regeneration.Thus,bio-piezoelectric materials have gained substantial attention in repairing damaged bone by mimicking the tissue’s ...Piezoelectricity in native bones has been well recognized as the key factor in bone regeneration.Thus,bio-piezoelectric materials have gained substantial attention in repairing damaged bone by mimicking the tissue’s electrical microenvironment(EM).However,traditional manufacturing strategies still encounter limitations in creating personalized bio-piezoelectric scaffolds,hindering their clinical applications.Three-dimensional(3D)/four-dimensional(4D)printing technology based on the principle of layer-by-layer forming and stacking of discrete materials has demonstrated outstanding advantages in fabricating bio-piezoelectric scaffolds in a more complex-shaped structure.Notably,4D printing functionality-shifting bio-piezoelectric scaffolds can provide a time-dependent programmable tissue EM in response to external stimuli for bone regeneration.In this review,we first summarize the physicochemical properties of commonly used bio-piezoelectric materials(including polymers,ceramics,and their composites)and representative biological findings for bone regeneration.Then,we discuss the latest research advances in the 3D printing of bio-piezoelectric scaffolds in terms of feedstock selection,printing process,induction strategies,and potential applications.Besides,some related challenges such as feedstock scalability,printing resolution,stress-to-polarization conversion efficiency,and non-invasive induction ability after implantation have been put forward.Finally,we highlight the potential of shape/property/functionality-shifting smart 4D bio-piezoelectric scaffolds in bone tissue engineering(BTE).Taken together,this review emphasizes the appealing utility of 3D/4D printed biological piezoelectric scaffolds as next-generation BTE implants.展开更多
Objective:To prepare the liposomes of mangrove oil,Optimization of the formulation of mangrove oil liposomes by Box Behnken response surface methodology.Methods:Preparation of Rhododendron oil liposomes by ethanol inj...Objective:To prepare the liposomes of mangrove oil,Optimization of the formulation of mangrove oil liposomes by Box Behnken response surface methodology.Methods:Preparation of Rhododendron oil liposomes by ethanol injection probe ultrasound,Determination of gemacrone by HPLC.The ratio of lecithin to cholesterol(X1),drug lipid ratio(X2)and phospholipid concentration(X3)were used as independent variables,and encapsulation efficiency(Y)was used as dependent variable,the formulation was optimized by Box Behnken response surface method,and the entrapment efficiency was predicted.The entrapment efficiency,particle size,polydispersity index(PDI),Zeta potential and drug loading of the optimized liposomes were evaluated.Results:The optimal prescription and preparation of Folium Rhododendri Daurici oil liposome was confirmed as follows:X1=7.28:1、X2=11.34:1、X3=9.32mg·mL-1,the encapsulation efficiency was(82.55±1.66)%,the particle size was(130.531±46)nm,the polydispersity index was 0.185±05,Zeta potential was(21.970±36)mV,the drug loading was(5.941±0.12)%.Conclusion:The Box Behnken response surface method is accurate to obtain the optimal formulation of mangrove oil liposomes,it has high precision and good prediction effect.And the preparation process of mangrove oil liposomes is stable and feasible.展开更多
A number of condensate reservoirs with high concentrations of H2S have been discovered in the deep dolomite reservoirs of the lower Ordovician Yingshan Formation（O1y） in the Tazhong Uplift, where the formation water...A number of condensate reservoirs with high concentrations of H2S have been discovered in the deep dolomite reservoirs of the lower Ordovician Yingshan Formation（O1y） in the Tazhong Uplift, where the formation water has a high p H value. In the O1y reservoir, the concentrations of Mg^2＋ and SO4^2-in the formation water are higher than those in the upper Ordovician formation.The concentration of H2 S in the condensate reservoirs and the concentration of Mg^2＋in the formation water correlate well in the O1y reservoirs of the Tazhong Uplift, which indicates a presumed thermochemical sulfate reduction（TSR） origin of H2S according to the oxidation theory of contact ion-pairs（CIPs）. Besides, the p H values of the formation water are positively correlated with the concentration of H2S in the condensate reservoirs, which may indicate that high p H might be another factor to promote and maintain TSR. Oil–source correlation of biomarkers in the sulfuretted condensates indicates the Cambrian source rocks could be the origin of condensates. The formation water in the condensate reservoirs of O1y is similar to that in the Cambrian; therefore, the TSR of sulfate-CIPs likelyoccurred in the Cambrian. High H2S-bearing condensates are mainly located near the No. 1 Fault and NE-SW strikeslip faults, which are the major migration pathway of deep fluids in the Tazhong Uplift. The redox between sulfateCIPs and hydrocarbons is the generation mechanism of H2S in the deep dolomite condensate reservoirs of the Tazhong Uplift. This finding should be helpful to predict the fluid properties of deep dolomite reservoirs.展开更多
BACKGROUND Drain-site hernia(DSH)has an extremely low morbidity and has rarely been reported.Small bowel obstruction is a frequent concurrent condition in most cases of DSH,which commonly occurs at the≥10 mm drain-si...BACKGROUND Drain-site hernia(DSH)has an extremely low morbidity and has rarely been reported.Small bowel obstruction is a frequent concurrent condition in most cases of DSH,which commonly occurs at the≥10 mm drain-site.Here we report a rare case of DSH at the lateral 5 mm port site one month postoperatively without visceral incarceration.Simultaneously,a brief review of the literature was conducted focusing on the risk factors,diagnosis,and prevention strategies for DSH.CASE SUMMARY A 76-year-old male patient was admitted to our institution with intermittent abdominal pain and a local abdominal mass which occurred one month after laparoscopic radical resection of rectal cancer one year ago.A computed tomography scan showed an abdominal wall hernia at the 5 mm former drain-site in the left lower quadrant,and that the content consisted of the large omentum.An elective herniorrhaphy was performed by closing the fascial defect and reinforcing the abdominal wall with a synthetic mesh simultaneously.The postoperative period was uneventful.The patient was discharged seven days after the operation without surgery-related complications at the 1-mo follow-up visit.CONCLUSION Emphasis should be placed on DSH despite the decreased use of intra-abdominal drainage.It is recommended that placement of a surgical drainage tube at the≥10 mm trocar site should be avoided.Moreover,it is advisable to have a comprehensive understanding of the risk factors for DSH and complete closure of the fascial defect at the drainage site for high-risk patients.展开更多
Donor shortages for organ transplantations are a major clinical challenge worldwide. Potential risks that are inevitably encountered with traditional methods include complications, secondary injuries, and limited sour...Donor shortages for organ transplantations are a major clinical challenge worldwide. Potential risks that are inevitably encountered with traditional methods include complications, secondary injuries, and limited source donors. Three-dimensional （3D） printing technology holds the potential to solve these limitations; it can he used to rapidly manufacture personalized tissue engineering scaffolds, repair tissue defects in situ with cells, and even directly print tissue and organs. Such printed implants and organs not only perfectly match the patient＇s damaged tissue, hut can also have engineered material microstructures and cell arrangements to promote cell growth and differentiation. Thus, such implants allow the desired tissue repair to he achieved, and could eventually solve the donor-shortage problem. This review summarizes relevant studies and recent progress on four levels, introduces different types of biomedical materials, and discusses existing problems and development issues with 3D printing that are related to materials and to the construction of extracellular matrix in vitro for medical applications.展开更多
An efficient immersed boundary-lattice Boltzmann method(IB-LBM)is proposed for fully resolved simulations of suspended solid particles in viscoelastic flows.Stress LBM based on Giesekus and Oldroyd-B constitutive equa...An efficient immersed boundary-lattice Boltzmann method(IB-LBM)is proposed for fully resolved simulations of suspended solid particles in viscoelastic flows.Stress LBM based on Giesekus and Oldroyd-B constitutive equation are used to model the viscoelastic stress tensor.A boundary thickening-based direct forcing IB method is adopted to solve the particle-fluid interactions with high accuracy for non-slip boundary conditions.A universal law is proposed to determine the diffusivity constant in a viscoelastic LBM model to balance the numerical accuracy and stability over a wide range of computa-tional parameters.An asynchronous calculation strategy is adopted to further improve the computing efficiency.The method was firstly applicated to the simulation of sedimentation of a single particle and a pair of particles after good validations in cases of the flow past a fixed cylinder and particle migration in a Couette flow against FEM and FVM methods.The determination of the asynchronous calculation strategy and the effect of viscoelastic stress distribution on the settling behaviors of one and two particles are revealed.Subsequently,504 particles settling in a closed cavity was simulated and the phenomenon that the viscoelastic stress stabilizing the Rayleigh-Taylor instabilities was observed.At last,simulations of a dense flow involving 11001 particles,the largest number of particles to date,were performed to investigate the instability behavior induced by elastic effect under hydrodynamic interactions in a viscoelastic fluid.The elasticity-induced ordering of the particle structures and fluid bubble structures in this dense flow is revealed for the first time.These simulations demonstrate the capability and prospects of the present method for aid in understanding the complex behaviors of viscoelastic particle suspensions.展开更多
Objective:To study the potential mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of cervical spondylosis based on the network pharmacological method,and to provide a basis for clinical application and phar...Objective:To study the potential mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of cervical spondylosis based on the network pharmacological method,and to provide a basis for clinical application and pharmacological research.Methods:TCMSP search was used to screen the main active compounds and related action targets of Huangqi Guizhi Wuwu Decoction,the standard names of action targets were obtained through the Uniprot database,and the"drug-target"interaction network was constructed by using Cytoscape3.6.1 software.The related targets of cervical spondylosis were obtained by searching GeneCards,OMIM,DisGeNET,and CTD databases,and the disease targets and drug targets were mapped by the Venny platform,and the intersection genes of them were the potential targets for drug treatment of this disease.Then,the intersection genes were input into the STRING database and Cytoscape 3.6.1 software,and the protein-protein interactions(PPI)network of"drug-disease intersection targets"was constructed,and we screened the core targets.Finally,GO biological function analysis and KEGG signaling pathway analysis of core targets were carried out through the DAVID database.Results:According to the screening conditions,74 effective compounds and 138 drug action targets of Huangqi Guizhi Wuwu Decoction were obtained,12179 genes related to cervical spondylosis were obtained,and the Venny platform analysis obtained 105 intersecting targets.With visual network processing by Cytoscape software,33 core targets were obtained by analyzing and calculating the core target degree values,which were JUN,PTGS2,NR3C1,TNF,IL6,TP53,EGFR,VEGFA,NOS3,IL1B,and so on.GO analysis showed that there were 133 items(P<0.05),involving adrenergic receptor activation,neurotransmitter receptor activation,nuclear receptor activation,catecholamine binding,etc.KEGG analysis obtained a total of 139 pathways(P<0.05),involving IL-17 signaling pathway,and tumor necrosis factor signaling pathway,relaxin signaling pathway,AGE-RAGE signaling pathway of diabetic complications,fluid shear stress,and atherosclerosis.Conclusion:the active components in Huangqi Guizhi Wuwu decoction may act on the core targets such as JUN,PTGS2,NR3C1,TNF,IL6,TP53,VEGFA through IL-17 signal pathway,tumor necrosis factor signal pathway,and AGE-RAGE signal pathway of diabetic complications,and exert their therapeutic effects.The research results can provide a basis for future basic and clinical research.展开更多
基金supported by grants from the National Natural Science Foundation of China(52205363)Fundamental Research Funds for the Central Universities(2019kfyRCPY044 and 2021GCRC002)+3 种基金Program for HUST Academic Frontier Youth Team(2018QYTD04)Program for Innovative Research Team of the Ministry of Education(IRT1244)Shenzhen-Hong Kong Science and Technology Innovation Cooperation Zone Shenzhen Park Project:HZQB-KCZYB-2020030the Guangdong Provincial Department of Science and Technology(Key-Area Research and Development Program of Guangdong Province)under the Grant 2020B090923002。
文摘Piezoelectricity in native bones has been well recognized as the key factor in bone regeneration.Thus,bio-piezoelectric materials have gained substantial attention in repairing damaged bone by mimicking the tissue’s electrical microenvironment(EM).However,traditional manufacturing strategies still encounter limitations in creating personalized bio-piezoelectric scaffolds,hindering their clinical applications.Three-dimensional(3D)/four-dimensional(4D)printing technology based on the principle of layer-by-layer forming and stacking of discrete materials has demonstrated outstanding advantages in fabricating bio-piezoelectric scaffolds in a more complex-shaped structure.Notably,4D printing functionality-shifting bio-piezoelectric scaffolds can provide a time-dependent programmable tissue EM in response to external stimuli for bone regeneration.In this review,we first summarize the physicochemical properties of commonly used bio-piezoelectric materials(including polymers,ceramics,and their composites)and representative biological findings for bone regeneration.Then,we discuss the latest research advances in the 3D printing of bio-piezoelectric scaffolds in terms of feedstock selection,printing process,induction strategies,and potential applications.Besides,some related challenges such as feedstock scalability,printing resolution,stress-to-polarization conversion efficiency,and non-invasive induction ability after implantation have been put forward.Finally,we highlight the potential of shape/property/functionality-shifting smart 4D bio-piezoelectric scaffolds in bone tissue engineering(BTE).Taken together,this review emphasizes the appealing utility of 3D/4D printed biological piezoelectric scaffolds as next-generation BTE implants.
基金Heilongjiang Province North Medicine and Functional Food Characteristic Discipline Construction Project(No.2018-TSXK-02)Heilongjiang Provincial Department of Education Project(No.12511574)。
文摘Objective:To prepare the liposomes of mangrove oil,Optimization of the formulation of mangrove oil liposomes by Box Behnken response surface methodology.Methods:Preparation of Rhododendron oil liposomes by ethanol injection probe ultrasound,Determination of gemacrone by HPLC.The ratio of lecithin to cholesterol(X1),drug lipid ratio(X2)and phospholipid concentration(X3)were used as independent variables,and encapsulation efficiency(Y)was used as dependent variable,the formulation was optimized by Box Behnken response surface method,and the entrapment efficiency was predicted.The entrapment efficiency,particle size,polydispersity index(PDI),Zeta potential and drug loading of the optimized liposomes were evaluated.Results:The optimal prescription and preparation of Folium Rhododendri Daurici oil liposome was confirmed as follows:X1=7.28:1、X2=11.34:1、X3=9.32mg·mL-1,the encapsulation efficiency was(82.55±1.66)%,the particle size was(130.531±46)nm,the polydispersity index was 0.185±05,Zeta potential was(21.970±36)mV,the drug loading was(5.941±0.12)%.Conclusion:The Box Behnken response surface method is accurate to obtain the optimal formulation of mangrove oil liposomes,it has high precision and good prediction effect.And the preparation process of mangrove oil liposomes is stable and feasible.
基金funded by the Natural Science Foundation of China (NSFC, Project No. 41473020)the CNPC International Cooperation Project (Grant No. 2011A-0203-01)
文摘A number of condensate reservoirs with high concentrations of H2S have been discovered in the deep dolomite reservoirs of the lower Ordovician Yingshan Formation（O1y） in the Tazhong Uplift, where the formation water has a high p H value. In the O1y reservoir, the concentrations of Mg^2＋ and SO4^2-in the formation water are higher than those in the upper Ordovician formation.The concentration of H2 S in the condensate reservoirs and the concentration of Mg^2＋in the formation water correlate well in the O1y reservoirs of the Tazhong Uplift, which indicates a presumed thermochemical sulfate reduction（TSR） origin of H2S according to the oxidation theory of contact ion-pairs（CIPs）. Besides, the p H values of the formation water are positively correlated with the concentration of H2S in the condensate reservoirs, which may indicate that high p H might be another factor to promote and maintain TSR. Oil–source correlation of biomarkers in the sulfuretted condensates indicates the Cambrian source rocks could be the origin of condensates. The formation water in the condensate reservoirs of O1y is similar to that in the Cambrian; therefore, the TSR of sulfate-CIPs likelyoccurred in the Cambrian. High H2S-bearing condensates are mainly located near the No. 1 Fault and NE-SW strikeslip faults, which are the major migration pathway of deep fluids in the Tazhong Uplift. The redox between sulfateCIPs and hydrocarbons is the generation mechanism of H2S in the deep dolomite condensate reservoirs of the Tazhong Uplift. This finding should be helpful to predict the fluid properties of deep dolomite reservoirs.
基金Supported by the Project of Health Commission of Hunan Province of ChinaNO. 20201853
文摘BACKGROUND Drain-site hernia(DSH)has an extremely low morbidity and has rarely been reported.Small bowel obstruction is a frequent concurrent condition in most cases of DSH,which commonly occurs at the≥10 mm drain-site.Here we report a rare case of DSH at the lateral 5 mm port site one month postoperatively without visceral incarceration.Simultaneously,a brief review of the literature was conducted focusing on the risk factors,diagnosis,and prevention strategies for DSH.CASE SUMMARY A 76-year-old male patient was admitted to our institution with intermittent abdominal pain and a local abdominal mass which occurred one month after laparoscopic radical resection of rectal cancer one year ago.A computed tomography scan showed an abdominal wall hernia at the 5 mm former drain-site in the left lower quadrant,and that the content consisted of the large omentum.An elective herniorrhaphy was performed by closing the fascial defect and reinforcing the abdominal wall with a synthetic mesh simultaneously.The postoperative period was uneventful.The patient was discharged seven days after the operation without surgery-related complications at the 1-mo follow-up visit.CONCLUSION Emphasis should be placed on DSH despite the decreased use of intra-abdominal drainage.It is recommended that placement of a surgical drainage tube at the≥10 mm trocar site should be avoided.Moreover,it is advisable to have a comprehensive understanding of the risk factors for DSH and complete closure of the fascial defect at the drainage site for high-risk patients.
文摘Donor shortages for organ transplantations are a major clinical challenge worldwide. Potential risks that are inevitably encountered with traditional methods include complications, secondary injuries, and limited source donors. Three-dimensional （3D） printing technology holds the potential to solve these limitations; it can he used to rapidly manufacture personalized tissue engineering scaffolds, repair tissue defects in situ with cells, and even directly print tissue and organs. Such printed implants and organs not only perfectly match the patient＇s damaged tissue, hut can also have engineered material microstructures and cell arrangements to promote cell growth and differentiation. Thus, such implants allow the desired tissue repair to he achieved, and could eventually solve the donor-shortage problem. This review summarizes relevant studies and recent progress on four levels, introduces different types of biomedical materials, and discusses existing problems and development issues with 3D printing that are related to materials and to the construction of extracellular matrix in vitro for medical applications.
基金supported by the National Natural Science Foundation of China(Grant Nos.51876075and 51876076).
文摘An efficient immersed boundary-lattice Boltzmann method(IB-LBM)is proposed for fully resolved simulations of suspended solid particles in viscoelastic flows.Stress LBM based on Giesekus and Oldroyd-B constitutive equation are used to model the viscoelastic stress tensor.A boundary thickening-based direct forcing IB method is adopted to solve the particle-fluid interactions with high accuracy for non-slip boundary conditions.A universal law is proposed to determine the diffusivity constant in a viscoelastic LBM model to balance the numerical accuracy and stability over a wide range of computa-tional parameters.An asynchronous calculation strategy is adopted to further improve the computing efficiency.The method was firstly applicated to the simulation of sedimentation of a single particle and a pair of particles after good validations in cases of the flow past a fixed cylinder and particle migration in a Couette flow against FEM and FVM methods.The determination of the asynchronous calculation strategy and the effect of viscoelastic stress distribution on the settling behaviors of one and two particles are revealed.Subsequently,504 particles settling in a closed cavity was simulated and the phenomenon that the viscoelastic stress stabilizing the Rayleigh-Taylor instabilities was observed.At last,simulations of a dense flow involving 11001 particles,the largest number of particles to date,were performed to investigate the instability behavior induced by elastic effect under hydrodynamic interactions in a viscoelastic fluid.The elasticity-induced ordering of the particle structures and fluid bubble structures in this dense flow is revealed for the first time.These simulations demonstrate the capability and prospects of the present method for aid in understanding the complex behaviors of viscoelastic particle suspensions.
文摘Objective:To study the potential mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of cervical spondylosis based on the network pharmacological method,and to provide a basis for clinical application and pharmacological research.Methods:TCMSP search was used to screen the main active compounds and related action targets of Huangqi Guizhi Wuwu Decoction,the standard names of action targets were obtained through the Uniprot database,and the"drug-target"interaction network was constructed by using Cytoscape3.6.1 software.The related targets of cervical spondylosis were obtained by searching GeneCards,OMIM,DisGeNET,and CTD databases,and the disease targets and drug targets were mapped by the Venny platform,and the intersection genes of them were the potential targets for drug treatment of this disease.Then,the intersection genes were input into the STRING database and Cytoscape 3.6.1 software,and the protein-protein interactions(PPI)network of"drug-disease intersection targets"was constructed,and we screened the core targets.Finally,GO biological function analysis and KEGG signaling pathway analysis of core targets were carried out through the DAVID database.Results:According to the screening conditions,74 effective compounds and 138 drug action targets of Huangqi Guizhi Wuwu Decoction were obtained,12179 genes related to cervical spondylosis were obtained,and the Venny platform analysis obtained 105 intersecting targets.With visual network processing by Cytoscape software,33 core targets were obtained by analyzing and calculating the core target degree values,which were JUN,PTGS2,NR3C1,TNF,IL6,TP53,EGFR,VEGFA,NOS3,IL1B,and so on.GO analysis showed that there were 133 items(P<0.05),involving adrenergic receptor activation,neurotransmitter receptor activation,nuclear receptor activation,catecholamine binding,etc.KEGG analysis obtained a total of 139 pathways(P<0.05),involving IL-17 signaling pathway,and tumor necrosis factor signaling pathway,relaxin signaling pathway,AGE-RAGE signaling pathway of diabetic complications,fluid shear stress,and atherosclerosis.Conclusion:the active components in Huangqi Guizhi Wuwu decoction may act on the core targets such as JUN,PTGS2,NR3C1,TNF,IL6,TP53,VEGFA through IL-17 signal pathway,tumor necrosis factor signal pathway,and AGE-RAGE signal pathway of diabetic complications,and exert their therapeutic effects.The research results can provide a basis for future basic and clinical research.
