miR-138调控年龄相关性白内障晶状体上皮细胞抗氧化应激作用的机制

目的:探讨miR-138对年龄相关性白内障晶状体上皮细胞抗氧化应激能力的影响及其作用机制。方法:采用实时定量PCR(RT-q PCR)检测年龄相关性白内障与正常人透明晶状体前囊膜组织中及人晶状体上皮细胞系(SRA01/04)氧化应激模型中miR-138的表达水平。利用2',7'-二氯荧光黄双乙酸盐(DCFH-DA)荧光探针检测细胞内源性活性氧(reactive oxygen species,ROS)水平。向人晶状体上皮细胞中分别转染miR-138 mimics,mimic controls,miR-138 inhibitors,inhibitor controls 72h后细胞暴露于400μmol/L H_2O_2 1h,采用RT-qPCR检测p53和Bax的mRNA表达,western blotting检测p53和Bax的蛋白表达水平,MTS法检测细胞增殖活力。结果:与正常对照组相比,年龄相关性白内障晶状体组织中与人晶状体上皮细胞氧化应激模型中miR-138的表达均显著升高,差异有统计学意义(P<0.001);人晶状体上皮细胞氧化应激模型中内源性ROS的水平明显升高,差异有统计学意义(P<0.001)。相对于对照组,miR-138 mimics组p53和Bax的mRNA、蛋白表达水平均明显升高,细胞增殖活力明显下降,差异有统计学意义(均P<0.001);miR-138 inhibitors组p53和Bax的mRNA、蛋白表达水平均明显下降,细胞增殖活力显著升高,差异有统计学意义(均P<0.001)。结论:miR-138在年龄相关性白内障囊膜组织中表达上调,通过正调控下游靶基因p53和Bax,下调人晶状体上皮细胞抗氧化应激的能力,抑制人晶状体上皮细胞增殖和修复,从而参与年龄相关性白内障的发生过程。

Circulating microRNAs,novel biomarkers of acute myocardial infarction:a systemic review

BACKGROUND:MicroRNAs(MiRNA) are a novel class of non-coding RNAs involved in the regulation of gene expression post-transcriptionally by cleavage or translational repression of their specific target miRNAs.Numerous studies have demonstrated that circulating miRNAs are stable and abundant in blood and aberrantly expressed under pathological conditions,including cardiovascular diseases.The implications of circulating miRNAs in acute myocardial infarction have recently been recognized.This review will highlight the potential role of miRNA as a novel class of biomarkers in acute myocardial infarction.METHODS:This systemic review is based on our own work and other related reports.RESULTS:During diseases circulating miRNAs are derived from not only circulating blood cells but also other tissues affected by ongoing diseases.These disease-related miRNAs in the blood can serve as potential biomarkers.CONCLUSION:The circulating miRNAs can be used as novel biomarkers potentially offering more sensitive and specific tests than those currently available for diagnosis of acute myocardial infarction.

改良ABC设计的非球面IOL植入对白内障患者术后视觉质量的影响

目的:评估改良非球面平衡曲线(ABC)设计的非球面人工晶状体(IOL)植入术后的视觉质量,同时进行临床IOL选择的影响因素分析,指导患者的IOL选择方案。方法:前瞻性病例对照研究。选取拟行白内障超声乳化联合折叠式IOL植入术的单纯性白内障患者67例74眼,其中观察组植入改良设计IOL 23眼(HOYA Vivinex XY1组),对照组植入传统设计IOL 51眼(Tecnis ZCB00组27眼,IQ SN60WF组24眼),术后1wk,1mo测量裸眼视力及最佳矫正视力、不同瞳孔直径下(3、4、5、6mm)全眼球差及彗差、不同瞳孔直径(3、4、5mm)下的调制传递函数(MTF)曲线、客观散射指数(OSI)、眼内散射光值Log(s)及对比敏感度。对所得的数据进行统计学分析。结果:三组术后1wk,1mo裸眼视力及最佳矫正视力均较术前显著提高,组间均无差异(P>0.05)。术后全眼球差值比较,术后1wk,5、6mm瞳孔直径下三组存在差异(P=0.045、0.037),术后1mo 6mm瞳孔直径下三组存在差异(P=0.042)。全眼彗差值比较,术后1wk,1mo 5、6mm瞳孔直径下三组均存在差异(P<0.05)。术后1wk,1mo,随瞳孔直径增加,Vivinex XY1组的全眼球差值增高程度小于其他两组,HOYA Vivinex XY1组在各个空间频率下的MTF值高于对照组,组间无差异(P>0.05);客观散射指数、眼内散射光值Log(s)及对比敏感度三组组间均无差异(P>0.05)。结论:改良设计的Vivinex IOL能够降低全眼球差及彗差,提高客观视觉质量,为改良式非球面IOL的选择提供了新的方案。

输尿管下段结石钬激光碎石术日间手术模式的可行性探讨

目的探讨输尿管下段结石钬激光碎石术日间手术模式的可行性。方法选取2019年1月—6月昆明医科大学第二附属医院输尿管下段结石患者85例作为研究对象,分为日间手术组(40例)和住院手术组(45例)。所有患者在硬膜外阻滞麻醉下完成手术,观察两组的等待手术时间、手术时间、住院时间、术后1个月结石清除率、住院费用、术后相关并发症和患者满意度。结果两组患者均成功完成手术,两组的等待手术时间、住院时间和住院费用比较,差异有统计学意义(P<0.05),而手术时间、术后1个月结石清除率及并发症比较,差异无统计学意义(P>0.05),日间手术组患者满意度总体比住院手术组高(P<0.05)。结论日间手术是一种安全有效的手术模式,符合目前提倡的快速康复理念。

窄带光谱成像膀胱软镜联合钬激光治疗非肌层膀胱癌的疗效观察

目的探讨窄带光谱成像膀胱软镜联合钬激光在非肌层浸润性膀胱癌(NMIBC)患者中的疗效。方法选取2017年6月—2018年6月于昆明医科大学第二附属医院泌尿外科收治的NMIBC患者60例。根据不同治疗方法随机分为窄带光谱成像组和白光成像组,各30例。比较两组手术相关指标和并发症情况,比较两组患者术后1个月、3个月、6个月及12个月的复发率,以及原位、异位肿瘤复发情况。结果窄带光谱成像组术后总复发率较白光成像组低(P<0.05)。白光成像组肿瘤原位复发率较窄带光谱成像组高(P<0.05)。术后肿瘤复发与年龄、肿瘤分级、肿瘤分期及膀胱多发肿瘤数目均呈正相关(rs=0.436、0.763、0.537和0.980,P<0.05)。两组围手术期并发症比较,差异无统计学意义(P>0.05)。两组术后1个月、3个月、6个月及12个月的复发率比较,差异无统计学意义(P>0.05)。结论窄带光谱成像膀胱软镜联合钬激光治疗NMIBC具有较好的疗效,安全性高,能够降低NMIBC患者术后复发率,具有临床应用的价值。

循环肿瘤细胞与泌尿系肿瘤关系的研究进展

循环肿瘤细胞(CTC)在肿瘤传播和转移中起重要作用,最终导致大多数癌症患者死亡。目前,从癌症患者血液中鉴定和计数CTC的技术已经将CTC确立为癌症诊断和预后的重要临床生物标志物。CTC具有巨大的潜力,作为非侵入性连续的液体活检技术,相比空间和时间限制的传统组织活检检测恶性肿瘤更精准。一旦CTC作为生物标志物用于泌尿系肿瘤患者的精确管理,CTC的临床应用将更广泛,广大癌症患者将明显受益。

An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis

MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division process remains unknown. Here, we report that an R2R3-type transcription factor gene, AtMYB59, is involved in the regulation of cell cycle progression and root growth. The AtMYB59 protein is localized in the nuclei of onion epidermal cells and has transactivation activity. Expression of AtMYB59 in yeast cells suppresses cell proliferation, and the transfor- mants have more nuclei and higher anenpioid DNA content with longer cells. Mutation in the conserved domain of AtMYB59 abolishes its effects on yeast cell growth. In synchronized Arabidopsis cell suspensions, the AtMYB59 gene is specifically expressed in the S phase during cell cycle progression. Expression and promoter-GUS analysis reveals that the AtMYB59 gene is abundantly expressed in roots. Transgenic plants overexpressing AtMYB59 have shorter roots compared with wild-type plants （Arabidopsis accession Col-0）, and around half of the mitotic cells in root tips are at metaphase. Conversely, the null mutant myb59-1 has longer roots and fewer mitotic cells at metaphase than Col, suggesting that AtMYB59 may inhibit root growth by extending the metaphase of mitotic cells. AtMYB59 regulates many downstream genes, including the CYCB1;1 gene, probably through binding to MYB-responsive elements. These results support a role forAtMYB59 in cell cycle regulation and plant root growth.

Evaluation of gastric lavage efficiency and utility using a rapid quantitative method in a swine paraquat poisoning model

BACKGROUND:Gastric lavage(GL)is one of the most critical early therapies for acute paraquat(PQ)poisoning;however,details of the treatment protocol remain to be established.METHODS:A rapid quantitative method involving sodium dithionite testing was developed.It was validated for the determination of the PQ concentrations in gastric juice and eluate samples from a swine acute PQ poisoning model with early or delay GL,or without.The vital signs,laboratory testing,and PQ plasma concentrations were collected for therapeutic effect evaluation.RESULTS:The reaction conditions of the test were optimized for two types of samples.Early GL at one hour(H1)could improve the signs and symptoms after acute PQ poisoning at 24 hours(H24).In contrast,GL at 6 hours(H6)could only partially relieve the vital signs.The H1 GL group effectively reduced the peak of the plasma PQ concentration.In addition,the PQ concentrations in the plasma and the gastric juice were significantly decreased in both the GL groups as compared to the untreated group at H24.Moreover,there was no significant difference in the washing efficiencies calculated from the total eluates between the two GL groups.However,the washing efficiency of the first 10 L eluate is superior to that of the additional 10 L eluate.CONCLUSION:GL only at early stage may it benefit PQ poisoning in an animal model.The currently used 20 L GL volume may need to be reduced in view of the low washing efficiency in the later 10 L eluate.The rapid quantitative method can be used for gastric juice sample and has a certain value for clinical GL practices.

Effects of hepatocyte growth factor on MMP-2 expression in scleral fibroblasts from a guinea pig myopia model

AIMTo investigate the effects of hepatocyte growth factor (HGF) on MMP-2 expression in scleral fibroblasts from guinea pig with LIM.

Visual function and higher order aberration after implantation of aspheric and spherical multifocal intraocular lenses:a meta-analysis

AIMTo assess the visual outcomes of aspheric multifocal intraocular lenses (IOLs) compared with spherical multifocal IOL after cataract surgery.

Effects of extracellular vesicles from mesenchymal stem cells on oxygen-glucose deprivation/reperfusioninduced neuronal injury

BACKGROUND: Small extracellular vesicles (sEVs) from bone marrow mesenchymal stemcells (BMSCs) have shown therapeutic potential for cerebral ischemic diseases. However, themechanisms by which BMSC-derived sEVs (BMSC-sEVs) protect neurons against cerebral ischemia/reperfusion (I/R) injury remain unclear. In this study, we explored the neuroprotective effects ofBMSC-sEVs in the primary culture of rat cortical neurons exposed to oxygen-glucose deprivation andreperfusion (OGD/R) injury.METHODS: The primary cortical neuron OGD/R model was established to simulate the processof cerebral I/R in vitro. Based on this model, we examined whether the mechanism through whichBMSC-sEVs could rescue OGD/R-induced neuronal injury.RESULTS: BMSC-sEVs (20 μg/mL, 40 μg/mL) significantly decreased the reactive oxygenspecies (ROS) productions, and increased the activities of superoxide dismutase (SOD) and glutathioneperoxidase (GPx). Additionally, BMSC-sEVs prevented OGD/R-induced neuronal apoptosis in vivo, asindicated by increased cell viability, reduced lactate dehydrogenase (LDH) leakage, decreased terminaldeoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining-positivecells, down-regulated cleaved caspase-3, and up-regulated Bcl-2/Bax ratio. Furthermore, Westernblot and flow cytometry analysis indicated that BMSC-sEV treatment decreased the expression ofphosphorylated calcium/calmodulin-dependent kinase II (p-CaMK II)/CaMK II, suppressed the increaseof intracellular calcium concentration ([Ca2+]i) caused by OGD/R in neurons.CONCLUSIONS: These results demonstrate that BMSC-sEVs have signifi cant neuroprotectiveeff ects against OGD/R-induced cell injury by suppressing oxidative stress and apoptosis, and Ca2+/CaMK II signaling pathways may be involved in this process.

Comparison of extracorporeal and conventional cardiopulmonary resuscitation:A meta-analysis of 2 260 patients with cardiac arrest

BACKGROUND: This meta-analysis aimed to determine whether extracorporeal cardiopulmonary resuscitation(ECPR), compared with conventional cardiopulmonary resuscitation(CCPR), improves outcomes in adult patients with cardiac arrest(CA).DATA RESOURCES: Pub Med, EMBASE, Web of Science, and China Biological Medicine Database were searched for relevant articles. The baseline information and outcome data(survival, good neurological outcome at discharge, at 3–6 months, and at 1 year after CA) were collected and extracted by two authors. Pooled risk ratios(RRs) and 95% confidence intervals(CIs) were calculated using Review Manager 5.3.RESULTS: In six studies 2 260 patients were enrolled to study the survival rate to discharge and longterm neurological outcome published since 2000. A signi? cant effect of ECPR was observed on survival rate to discharge compared to CCPR in CA patients(RR 2.37, 95%CI 1.63–3.45, P<0.001), and patients who underwent ECPR had a better long-term neurological outcome than those who received CCPR(RR 2.79, 95%CI 1.96–3.97, P<0.001). In subgroup analysis, there was a significant difference in survival to discharge favoring ECPR over CCPR group in OHCA patients(RR 2.69, 95%CI 1.48–4.91, P=0.001). However, no signi? cant difference was found in IHCA patients(RR 1.84, 95%CI 0.91–3.73, P=0.09).CONCLUSION: ECPR showed a bene? cial effect on survival rate to discharge and long-term neurological outcome over CCPR in adult patients with CA.

miR-211 regulates the antioxidant function of lens epithelial cells affected by age-related cataracts

AIM： To investigate the effects and mechanism of miR-211 in mediating the antioxidant function of lens epithelial cells affected by age-related cataracts. METHODS： Real-time quantitative polymerase chain reaction （RT-qPCR） was used to detect miR-211 expression in the anterior lens capsules of healthy people, the anterior lens capsules of patients with age-related cataracts, and human epithelial cell line （SRA01/04） cells exposed to oxidative stress. A 2＇, 7＇-dichloro-fluorescein diacetate （DCFH-DA） probe was used to measure the levels of endogenous reactive oxygen species （ROS） in human lens epithelial cells （hLECs） exposed to 400 pmol/L H2O2 for lh. SRA01/04 cells were transfected with either miR-211 mimics, mimic controls, miR-211 inhibitors or inhibitor controls. After 72h, these cells were exposed to 400 IJmollL H2O2 for lh, then p53 and Bax mRNA expression were measured using RT-qPCR. p53 and Bax protein expression were also measured by Western blotting analysis. Finally, cell viability was assessed using an MTS assay. RESULTS： Compared to the control group, expression of miR-211 in the anterior lens capsules of age-related cataract patients and in SRA01/04 cells exposed to oxidative stress was significantly increased （P〈0.001）. Levels of endogenous ROS were significantly elevated in hLECs exposed to oxidative stress （P〈0.001）. Compared to the mimic control group, the hLECs in the miR-211 mimic group expressed significantly higher levels of p53 and Bax mRNA and protein while cell viability was significantly reduced （P〈0.001）. Conversely, p53 and Bax mRNA and protein expression were significantly reduced in the miR-211 inhibitor group as compared to the control group, while the cells in this group had much higher levels of call viability （P〈0.001）. CONCLUSION： miR-211 is upregulatsd in the anterior lens capsules of age-related cataract patients, miR-211 decreased the antioxidative stress capacity of lens epithelial cells by upregulating p53 and Bax, while inhibiting cell proliferation and repair. This finding suggests that miR-211 may play a key role in the development of age-related cataracts.

A case of thyroid emergency with cardiac arrest supported by extracorporeal membrane oxygenation

Dear editor,Thyroid emergency is a rare but potentially life-threatening condition if not recognized early and managed properly.It is usually due to a severe exacerbation of a preexisting thyrotoxicosis,which later leads to decompensation in different organ systems.The treatment of thyroid emergency remains challenging even with the armamentarium of modern intensive care technologies,especially in patients with cardiac

Distance vision after bilateral implantation of AcrySof toric intraocular lenses:a randomized,controlled,prospective trial

·AIM:To evaluate the distance vision of Chinese patients with cataracts and corneal astigmatism after implantation of bilateral AcrySof toric intraocular lens (IOL) versus bilateral AcrySof spherical IOL.·METHODS:This study randomized 60 patients into equal groups to receive toric IOL or spherical IOL.IOL powers targeting emmetropia were selected for 93% of toric IOL patients and for 90% of spherical IOL patients.Assessments included monocular and binocular distance vision,with and without best correction.Patients also completed surveys about their distance vision.·RESULTS:Preoperatively,the two study groups were similar in age,in distance visual acuity,and in the magnitude of corneal astigmatism.At 6 months postoperative,binocular uncorrected distance vision was 0.06?à0.14 logMAR in the AcrySof toric IOL group,significantly better than the 0.14?à0.11 logMAR in the spherical IOL group (P<0.05).For eyes with emmetropia as a target,the equivalent of 20/20 uncorrected vision was more likely (P<0.001) in the toric IOL group (36% of eyes) than in the spherical IOL group (4% of eyes).No patients in the emmetropia/toric IOL group used distance glasses,as compared to 52% of patients in the emmetropia/spherical IOL group.All patients were satisfied or highly satisfied.Quality of distance vision was rated higher by toric IOL patients than by spherical IOL patients (P<0.05).·CONCLUSION:Bilateral AcrySof toric IOL is superior to bilateral spherical IOL in providing uncorrected distance vision to cataract patients with corneal astigmatism.·

SUMOylation and deacetylation affect NF-κB p65 activity induced by high glucose in human lens epithelial cells

AIM: To explore the effects of IκBα SUMOylation and NF-κB p65 deacetylation on NF-κB p65 activity induced by high glucose in cultured human lens epithelial cells(HLECs).METHODS: HLECs(SRA01/04) were cultured with 5.5, 25, and 50 mmol/L glucose media for 24 h, and with 50 mmol/L glucose media for 0, 12, and 24 h respectively. SUMO1 and SIRT1 expressions were detected by reverse transcriptionpolymerase chain reaction(RT-PCR) and Western blot(WB). IκBα and NF-κB p65 expressions were detected by WB. With NAC, DTT, MG132 or Resveratrol(RSV) treatment, SUMO1 and SIRT1 expressions were detected by WB. Protein expression localizations were examined by immunofluorescence and co-immunofluorescence. The effects of SUMO1 or SIRT1 overexpression, as well as MG132 and RSV, on the nuclear expression and activity of IκBα and NF-κB p65 were analyzed by immunoblot and dual luciferase reporter gene assay.RESULTS: SUMO1 and SIRT1 expressions were influenced by high glucose in mRNA and protein levels, which could be blocked by NAC or DTT. SUMO1 was down-regulated by using MG132, and SIRT1 was up-regulated under RSV treatment. IκBα nuclear expression was attenuated and NF-κB p65 was opposite under high glucose, while IκBα and NF-κB p65 location was transferred to the nucleus. SUMO1 or SIRT1 overexpression and MG132 or RSV treatment affected the nuclear expression and activity of IκBα and NF-κB p65 under high glucose condition.CONCLUSION: IκBα SUMOylation and NF-κB p65 deacetylation affect NF-κB p65 activity in cultured HLECs under high glucose, and presumably play a significant role in controlling diabetic cataract.

Mechanical cardiopulmonary resuscitation for patients with cardiac arrest

BACKGROUND：Although modern cardiopulmonary resuscitation （CPR） substantially decreases the mortality induced by cardiac arrest, cardiac arrest still accounts for over 50% of deaths caused by cardiovascular diseases. In this article, we address the current use of mechanical devices during CPR, and also compare the CPR quality between manual and mechanical chest compression. METHODS：We compared the quality and survival rate between manual and mechanical CPR, and then reviewed the mechanical CPR in special circumstance, such as percutaneous coronary intervention, transportation, and other fields. RESULTS：Compared with manual compression, mechanical compression can often be done correctly, and thus can compromise survival; can provide high quality chest compressions in a moving ambulance; enhance the flow of blood back to the heart via a rhythmic constriction of the veins; allow ventilation and CPR to be per formed simultaneously. CONCLUSION：Mechanical devices will be widely used in clinical practice so as to improve the quality of CPR in patients with cardiac arrest.

Bibliometric analysis and mapping knowledge domain of pterygium:2000-2019

AIM:To track the knowledge structure,topics in focus,and trends in emerging research in pterygium in the past 20 y.METHODS:Base on the Web of Science Core Collection(Wo SCC),studies related to pterygium in the past 20 y from 2000-2019 have been included.With the help of VOSviewer software,a knowledge map was constructed and the distribution of countries,institutions,journals,and authors in the field of pterygium noted.Meanwhile,using cocitation analysis of references and co-occurrence analysis of keywords,we identified basis and hotspots,thereby obtaining an overview of this field.RESULTS:The search retrieved 1516 publications from Wo SCC on pterygium published between 2000 and 2019.In the past two decades,the annual number of publications is on the rise and fluctuated a little.Most productive institutions are from Singapore but the most prolific and active country is the United States.Journal Cornea published the most articles and Coroneo MT contributed the most publications on pterygium.From cooccurrence analysis,the keywords formed 3 clusters:1)surgical therapeutic techniques and adjuvant of pterygium,2)occurrence process and pathogenesis of pterygium,and 3)epidemiology,and etiology of pterygium formation.These three clusters were consistent with the clustering in co-citation analysis,in which Cluster 1 contained the most references(74 publications,47.74%),Cluster 2 contained 53 publications,accounting for 34.19%,and Cluster 3 focused on epidemiology with 18.06%of total 155 cocitation publications.CONCLUSION:This study demonstrates that the research of pterygium is gradually attracting the attention of scholars and researchers.The interaction between authors,institutions,and countries is lack of.Even though,the research hotspot,distribution,and research status in pterygium in this study could provide valuable information for scholars and researchers.

Anterior segment dysgenesis correlation with epithelial-mesenchymal transition in Smad4 knockout mice

AIM： To explore the molecular mechanisms in lens development and the pathogenesis of Peters anomaly in Smad4 defective mice. METHODS： Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ectoderm selectively. Pathological techniques were used to reveal the morphological changes of the anterior segment in Smad4 defective eye. Immunohistochemical staining was employed to observe the expression of E-cadherin, N- cadherin and α-SMA in anterior segment of Smad4 defective mice and control mice at embryonic （E） day 16.5. Real-time quantitative polymerase chain reaction （qPCR） was performed to detect the expression of Snail, Zebl, Zeb2 and Twist2 in lens of Smad4 defective mice and control mice at E16.5. RESULTS： Conditional deletion of Smad4 on eye surface ectoderm resulted in corneal dysplasia, iridocorneal angle closure, corneolenticular adhesions and cataract resembling Peters anomaly. Loss of Smad4 function inhibited E-cadherin expression in the lens epithelium cells and corneal epithelium cells in Smad4 defective eye. Expression of N-cadherin was upregulated in corneal epithelium and corneal stroma. Both E-cadherin and N-cadherin were down-regulated at the future trabecular meshwork region in mutant eye. The qPCR results showed that the expression of Twist2 was increased significantly in the mutant lens （P〈0.01）. CONCLUSION： Smad4 is essential to eye development and likely a candidate pathogenic gene to Peters anomaly by regulating epithelial-mesenchymal transition. Twist2 can be regulated by Smad4 and plays an essential role in lens development.

Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery

AIM： To evaluate human lens epithelium cell apoptosis and epithelial to mesenchymal transition （EMT） induced by femtosecond laser in femtosecond laser assisted cataract surgery （FLACS）. METHODS： Sixty cataract patients with N2 to N3 stage according to the LOCS III were enrolled in this study and divided into three groups randomly： FLACSl group （cataract surgery by FLACS with LenSx）, FLACS2 group （cataract surgery by FLACS with LensAR） and manual group （cataract surgery by phacoemulsification）. Patients in two FLACS groups performed anterior capsulotomy by LenSx or LensAR laser system. Patients in the manual group were performed continuous curvilinear capsulorrhexis （CCC） manually. The anterior capsules were fixed right after moved out of eye. Hematoxylin-eosine staining, immunofluorescence staining and real-time PCR were performed in order to observe human lens epithelium cells changes after cataract surgery. RESULTS： The capsule cutting edge was shown irregularity and roughness in two FLACS groups and smooth edge in manual capsulotomy by pathologic staining. Irregularities of the cell configuration with partly swollen and destroyed nuclei were observed in two FLACS groups. Femtosecond laser could induce a significantly higher cell apoptosis in human lens epithelium cell than manually performed CCC （P〈0.05）. Lens epithelium cells apoptosis were correlated with femtosecond laser duration according to Pearson correlation analysis. Decreased N-cadherin expression, alpha-SMA and FSP-1 level in two FLACS groups showed the inhibition of cell EMT. CONCLUSION： Femtosecond laser may affect the apoptosis and EMT of lens epithelium cells which are under the peeled central lens capsule.