Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive deterioration.The presence of the blood-brain barrier presents a major obstacle to the dev...Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive deterioration.The presence of the blood-brain barrier presents a major obstacle to the development of effective drug therapies for Alzheimer’s disease.The use of ultrasound as a novel physical modulation approach has garnered widespread attention in recent years.As a safe and feasible therapeutic and drug-delivery method,ultrasound has shown promise in improving cognitive deficits.This article provides a summary of the application of ultrasound technology for treating Alzheimer’s disease over the past 5 years,including standalone ultrasound treatment,ultrasound combined with microbubbles or drug therapy,and magnetic resonance imaging-guided focused ultrasound therapy.Emphasis is placed on the benefits of introducing these treatment methods and their potential mechanisms.We found that several ultrasound methods can open the blood-brain barrier and effectively alleviate amyloid-βplaque deposition.We believe that ultrasound is an effective therapy for Alzheimer’s disease,and this review provides a theoretical basis for future ultrasound treatment methods.展开更多
The three rural issues of agriculture, rural areas, and farmers are important strategic issues in China’s development, are an important foundation for the agricultural country to realize the modernization of agricult...The three rural issues of agriculture, rural areas, and farmers are important strategic issues in China’s development, are an important foundation for the agricultural country to realize the modernization of agriculture and rural areas and realize the great rejuvenation of the Chinese nation. Under the new situation of great victory in poverty alleviation, it is necessary to protect the fruits of victory and strengthen the construction of new countryside in the new era. From the current development situations of agriculture, rural areas, and farmers, this paper explored the development trend of three elements, to provide a feasible path for protecting the achievements in poverty alleviation, and provide certain ideas for workers engaged in agriculture, rural areas, and farmers.展开更多
Nowadays,the rapid development of the social economy inevitably leads to global energy and environmental crisis.For this reason,more and more scholars focus on the development of photocatalysis and/or electrocatalysis...Nowadays,the rapid development of the social economy inevitably leads to global energy and environmental crisis.For this reason,more and more scholars focus on the development of photocatalysis and/or electrocatalysis technology for the advantage in the sustainable production of high-value-added products,and the high efficiency in pollutants remediation.Although there is plenty of outstanding research has been put forward continuously,most of them focuses on catalysis performance and reaction mechanisms in laboratory conditions.Realizing industrial application of photo/electrocatalytic processes is still a challenge that needs to be overcome by social demand.In this regard,this review comprehensively summarized several explorations in thefield of photo/electrocatalytic reduction towards potential industrial applications in recent years.Special attention is paid to the successful attempts and the current status of photo/electrocatalytic water splitting,carbon dioxide conversion,resource utilization from waste,etc.,by using advanced reactors.The key problems and challenges of photo/electrocatalysis in future industrial practice are also discussed,and the possible development directions are also pointed out from the industry view.展开更多
A series of model catalysts were obtained by treating commercial fresh and spent catalysts unloaded from the factory with different methods, including green oil dipping, extraction and high-temperature regeneration;fi...A series of model catalysts were obtained by treating commercial fresh and spent catalysts unloaded from the factory with different methods, including green oil dipping, extraction and high-temperature regeneration;finally, the deactivation behavior of the commercial catalyst for acetylene hydrogenation were studied. The influence of various possible deactivation factors on the catalytic performance was elucidated via detailed structural characterization, surface composition analysis, and activity evaluation.The results showed that green oil, carbon deposit and sintering of active metal were the main reasons for deactivation, among which green oil and carbon deposit led to rapid deactivation, while the activity could be recovered after regeneration by high-temperature calcination. The sintering of active metal components was attributed to the high-temperature regeneration in hydrothermal conditions, which was slow but irreversible and accounted for permanent deactivation. Thus, optimizing the regeneration is expected to extend the service life of the commercial catalyst.展开更多
Lung cancer is the most common and fatal malignant disease worldwide and has the highest mortality rate among tumor-related causes of death.Early diagnosis and precision medicine can significantly improve the survival...Lung cancer is the most common and fatal malignant disease worldwide and has the highest mortality rate among tumor-related causes of death.Early diagnosis and precision medicine can significantly improve the survival rate and prognosis of lung cancer patients.At present,the clinical diagnosis of lung cancer is challenging due to a lack of effective non-invasive detection methods and biomarkers,and treatment is primarily hindered by drug resistance and high tumor heterogeneity.Liquid biopsy is a method for detecting circulating biomarkers in the blood and other body fluids containing genetic information from primary tumor tissues.Bronchoalveolar lavage fluid(BALF)is a potential liquid biopsy medium that is rich in a variety of bioactive substances and cell components.BALF contains information on the key characteristics of tumors,including the tumor subtype,gene mutation type,and tumor environment,thus BALF may be used as a diagnostic supplement to lung biopsy.In this review,the current research on BALF in the diagnosis,treatment,and prognosis of lung cancer is summarized.The advantages and disadvantages of different components of BALF,including cells,cell-free DNA,extracellular vesicles,and micro RNA are introduced.In particular,the great potential of extracellular vesicles in precision diagnosis and detection of drug-resistant for lung cancer is highlighted.In addition,the performance of liquid biopsies with different body fluid sources in lung cancer detection are compared to facilitate more selective studies involving BALF,thereby promoting the application of BALF for precision medicine in lung cancer patients in the future.展开更多
The mouse genome has a high degree of homology with the human genome,and its physiological,biochemical,and developmental regulation mechanisms are similar to those of humans;therefore,mice are widely used as experimen...The mouse genome has a high degree of homology with the human genome,and its physiological,biochemical,and developmental regulation mechanisms are similar to those of humans;therefore,mice are widely used as experimental animals.However,it is undeniable that interspecies differences between humans and mice can lead to experimental errors.The differences in the immune system have become an impor-tant factor limiting current immunological research.The application of immunodefi-cient mice provides a possible solution to these problems.By transplanting human immune cells or tissues,such as peripheral blood mononuclear cells or hematopoietic stem cells,into immunodeficient mice,a human immune system can be reconstituted in the mouse body,and the engrafted immune cells can elicit human-specific immune responses.Researchers have been actively exploring the development and differen-tiation conditions of host recipient animals and grafts in order to achieve better im-mune reconstitution.Through genetic engineering methods,immunodeficient mice can be further modified to provide a favorable developmental and differentiation microenvironment for the grafts.From initially only being able to reconstruct single T lymphocyte lineages,it is now possible to reconstruct lymphoid and myeloid cells,providing important research tools for immunology-related studies.In this review,we compare the differences in immune systems of humans and mice,describe the devel-opment history of human immune reconstitution from the perspectives of immuno-deficient mice and grafts,and discuss the latest advances in enhancing the efficiency of human immune cell reconstitution,aiming to provide important references for im-munological related researches.展开更多
目的观察七氟醚和丙泊酚对危重患儿肺功能的影响。方法选择行腹部手术合并肺炎危重患儿64例,男39例,女25例,日龄1~28 d,体重2~5 kg,ASAⅢ级。采用随机数字表法将患儿分为两组:七氟醚吸入麻醉组(S组)和丙泊酚静脉麻醉组(P组),每组32例。...目的观察七氟醚和丙泊酚对危重患儿肺功能的影响。方法选择行腹部手术合并肺炎危重患儿64例,男39例,女25例,日龄1~28 d,体重2~5 kg,ASAⅢ级。采用随机数字表法将患儿分为两组:七氟醚吸入麻醉组(S组)和丙泊酚静脉麻醉组(P组),每组32例。记录插管后30 min、术毕、术后6 h的氧合指数(OI)、肺泡动脉氧分压差(PA-aO_(2))、呼吸指数(RI)、血清髓系细胞表达触发受体-1(TREM-1)、白细胞介素-8(IL-8)浓度。记录低氧血症、心律失常、严重感染、新生儿呼吸窘迫、无法纠正的低血压等围术期不良事件的发生情况。结果与插管后30 min比较,S组术毕、术后6 h OI明显升高,PA-aO_(2)和RI明显降低(P<0.05);P组术毕OI、术后6 h PA-aO_(2)明显降低(P<0.05)。与术毕比较,S组术后6 h OI明显升高,RI明显降低(P<0.05),P组术后6 h PA-aO_(2)明显降低(P<0.05)。与P组比较,术毕、术后6 h S组OI明显升高,RI明显降低,术后6 h PA-aO_(2)明显升高(P<0.05)。两组不同时点血清TREM-1浓度差异无统计学意义。与术毕比较,P组术后6 h血清IL-8浓度明显降低(P<0.05)。与P组比较,S组术毕、术后6 h血清IL-8浓度明显降低(P<0.05)。两组患儿均无围术期不良事件发生。结论与丙泊酚比较,七氟醚能够降低危重患儿血清炎性因子IL-8浓度,对危重患儿具有肺保护作用。展开更多
AIM: To determine whether the combination of platelet count(PLT) with spleen volume parameters and right liver volume(RV) measured by magnetic resonance imaging(MRI) could predict the Child-Pugh class of liver cirrhos...AIM: To determine whether the combination of platelet count(PLT) with spleen volume parameters and right liver volume(RV) measured by magnetic resonance imaging(MRI) could predict the Child-Pugh class of liver cirrhosis and esophageal varices(EV).METHODS: Two hundred and five cirrhotic patients with hepatitis B and 40 healthy volunteers underwent abdominal triphasic-enhancement MRI and laboratory examination of PLT in 109/L. Cirrhotic patients underwent endoscopy for detecting EV. Spleen maximal width(W), thickness(T) and length(L) in mm together with spleen volume(SV) and RV in mm3 were measured by MRI, and spleen volume index(SI) in mm3 was obtained by W × T × L. SV/PLT, SI/PLT and RV × PLT/SV(RVPS) were calculated and statistically analyzed to assess cirrhosis and EV.RESULTS: SV/PLT(r = 0.676) and SI/PLT(r = 0.707) increased, and PLT(r =-0.626) and RVPS(r =-0.802) decreased with the progress of Child-Pugh class(P < 0.001 for all). All parameters could determine the presence of cirrhosis, distinguish between each class of Child-Pugh class, and identify the presence of EV [the areas under the curve(AUCs) = 0.661-0.973]. A m o n g p a ra m e t e r s, R V P S c o u l d b e s t d e t e r m i n e presence and each class of cirrhosis with AUCs of 0.973 and 0.740-0.853, respectively; and SV/PLT could best identify EV with an AUC of 0.782.CONCLUSION: The combination of PLT with SV and RV could predict Child-Pugh class of liver cirrhosis and identify the presence of esophageal varices.展开更多
High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neur...High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.展开更多
AIM: To study the therapeutic effect of norcantharidin(NCTD) combined with ABT-737 on hepatocellular carcinoma cells and the molecular mechanism. METHODS: Two hepatocellular carcinoma(HCC) cell lines, Hep G2 and SMMC-...AIM: To study the therapeutic effect of norcantharidin(NCTD) combined with ABT-737 on hepatocellular carcinoma cells and the molecular mechanism. METHODS: Two hepatocellular carcinoma(HCC) cell lines, Hep G2 and SMMC-7721, were selected. ABT-737 and NCTD were allocated into groups to be used alone or in combination. Hep G2 and SMMC-7721 cells were cultured in vitro. Liver cancer cells in the logarithmic phase of growth were vaccinated and cultured to the cell wall stage; these cells were treated for 48 h with different concentrations of NCTD, or ABT-737, or NCTD combined with ABT-737. The cell proliferation inhibition rate was detected by methyl thiazolyl tetrazolium. The expression of Mcl in HCC cells was detected by Western Blotting, and the cells in each group after treatment had apoptosis detected by flow cytometry. The proliferation inhibition rate, the expression of Mcl-1 in cells and the apoptosis inducing effect of treatment were observed in each group, and the effect of NCTD on ABT-737 in the treatment of HCC and its mechanism of action were analyzed.RESULTS: As the concentration of NCTD increased, the cell proliferation inhibition rate gradually decreased; and the treatment effect of ABT-737 1-3 μm combined with NCTD on cell proliferation inhibition was stronger than that of ABT-737 alone. The difference was statistically significant(P < 0.05). In observing the expression of Mcl-1 in cells after the treatment of different concentrations of NCTD, this was partiallyinhibited after treatment with NCTD 15 μm, and the expression of Mcl-1 was almost undetectable after treatment with NCTD 30 μm and 60 μm. The effect on inducing apoptosis with the treatment of ABT-737 or NCTD alone for 48 h was lower than that of the control group. The difference was not statistically significant(P > 0.05). The effect on inducing apoptosis in Hep G2 and SMMC-7721 cells with the treatment of ABT-737 combined with NCTD for 48 h was greater than that of ABT-737 or NCTD alone. The difference was statistically significant(P < 0.05). CONCLUSION: NCTD combined with ABT-737 has a positive role in the treatment of HCC, and it has great value in clinical research.展开更多
Blindness and vision impairment are the most devastating global health problems resulting in a substantial economic and social burden.Delivery of drug to particular parts of the anterior or posterior segment has been ...Blindness and vision impairment are the most devastating global health problems resulting in a substantial economic and social burden.Delivery of drug to particular parts of the anterior or posterior segment has been a major challenge due to various protective barriers and elimination mechanisms associated with the unique anatomical and physiological nature of the ocular system.Drug administration to the eye by conventional delivery systems results in poor ocular bioavailability(<5%).The designing of a novel approach for a safe,simple,and effective ocular drug delivery is a major concern and requires innovative strategies to combat the problem.Over the past decades,several novel approaches involving different strategies have been developed to improve the ocular delivery system.Among these,the ophthalmic in-situ gel has attained a great attention over the past few years.This review discussed and summarized the recent and the promising research progress of in-situ gelling in ocular drug delivery system.展开更多
With the increasing demand for electricity,an efficiency improvement and thereby reduced CO2 emissions of the coal-fired plants are expected in order to reach the goals set in the Kyoto protocol.It can be achieved by ...With the increasing demand for electricity,an efficiency improvement and thereby reduced CO2 emissions of the coal-fired plants are expected in order to reach the goals set in the Kyoto protocol.It can be achieved by a rise of the process parameters.Currently,live steam pressures and temperatures up to 300 bars and 923 K are planned as the next step.Closed circuit steam cooling of blades and vanes in modern steam turbines is a promising technology in order to establish elevated live steam temperatures in future steam turbine cycles.In this paper,a steam-cooled test vane in a cascade with external hot steam flow is analyzed numerically with the in-house code CHTflow.A parametric analysis aiming to improve the cooling effectiveness is carried out by varying the cooling mass flow ratio.The results from two investigated cases show that the steam cooling technique has a good application potential in the steam turbine.The internal part of the vane is cooled homogeneously in both cases.With the increased cooling mass flow rate,there is a significant improvement of cooling efficiency at the leading edge.The results show that the increased cooling mass flow ratio can enhance the cooling effectiveness at the leading edge.With respect to trailing edge,there is no observable improvement of cooling effectiveness with the increased cooling mass flow.This implies that due to the limited dimension at the trailing edge,the thermal stress cannot be decreased by increasing the cooling mass flow rate.Therefore,impingement-cooling configuration at the trailing edge might be a solution to overcome the critical thermal stress there.It is also observed that the performance of the cooling effective differs on pressure side and suction side.It implicates that the equilibrium of the cooling effectiveness on two sides are influenced by a coupled relationship between cooling mass flow ratio and hole geometry.In future work,optimizing the hole geometry and cooling steam supply conditions might be the solutions for an equivalent cooling effectiveness along whole profile.展开更多
We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding ...We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.展开更多
Silks have various advantages compared with synthetic polymer fibers,such as sustainability,mechanical properties,luster,as well as air and humidity permeability.However,the functionalization of silks has not yet been...Silks have various advantages compared with synthetic polymer fibers,such as sustainability,mechanical properties,luster,as well as air and humidity permeability.However,the functionalization of silks has not yet been fully developed.Functionalization techniques that retain or even improve the sustainability of silk production are required.To this end,a low-cost,effective,and scalable strategy to produce TCSs by integrating yarn-spinning and continuous dip coating technique is developed herein.TCSs with extremely long length(>10 km),high mechanical performance(strength of 443.1 MPa,toughness of 56.0 MJ m−3,comparable with natural cocoon silk),and good interfacial bonding were developed.TCSs can be automatically woven into arbitrary fabrics,which feature super-hydrophobicity as well as rapid and programmable thermochromic responses with good cyclic performance:the response speed reached to one second and remained stable after hundreds of tests.Finally,applications of TCS fabrics in temperature management and dynamic textile displays are demonstrated,confirming their application potential in smart textiles,wearable devices,flexible displays,and human–machine interfaces.Moreover,combination of the fabrication and the demonstrated applications is expected to bridge the gap between lab research and industry and accelerate the commercialization of TCSs.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82371886(to JY),81925020(to DM),82202797(to LW),and 82271218(to CZ).
文摘Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive deterioration.The presence of the blood-brain barrier presents a major obstacle to the development of effective drug therapies for Alzheimer’s disease.The use of ultrasound as a novel physical modulation approach has garnered widespread attention in recent years.As a safe and feasible therapeutic and drug-delivery method,ultrasound has shown promise in improving cognitive deficits.This article provides a summary of the application of ultrasound technology for treating Alzheimer’s disease over the past 5 years,including standalone ultrasound treatment,ultrasound combined with microbubbles or drug therapy,and magnetic resonance imaging-guided focused ultrasound therapy.Emphasis is placed on the benefits of introducing these treatment methods and their potential mechanisms.We found that several ultrasound methods can open the blood-brain barrier and effectively alleviate amyloid-βplaque deposition.We believe that ultrasound is an effective therapy for Alzheimer’s disease,and this review provides a theoretical basis for future ultrasound treatment methods.
基金Supported by National Undergraduate Training Program for Innovation and Entrepreneurship of the Ministry of Education (202114389010)。
文摘The three rural issues of agriculture, rural areas, and farmers are important strategic issues in China’s development, are an important foundation for the agricultural country to realize the modernization of agriculture and rural areas and realize the great rejuvenation of the Chinese nation. Under the new situation of great victory in poverty alleviation, it is necessary to protect the fruits of victory and strengthen the construction of new countryside in the new era. From the current development situations of agriculture, rural areas, and farmers, this paper explored the development trend of three elements, to provide a feasible path for protecting the achievements in poverty alleviation, and provide certain ideas for workers engaged in agriculture, rural areas, and farmers.
基金supported by the National Natural Science Foundation of China(22278030,22090032,22090030,22288102,22242019)the Fundamental Research Funds for the Central Universities(buctrc202119,2312018RC07)+1 种基金Major Program of Qingyuan Innovation Laboratory(Grant No.001220005)the Experiments for Space Exploration Program and the Qian Xuesen Laboratory,China Academy of Space Technology。
文摘Nowadays,the rapid development of the social economy inevitably leads to global energy and environmental crisis.For this reason,more and more scholars focus on the development of photocatalysis and/or electrocatalysis technology for the advantage in the sustainable production of high-value-added products,and the high efficiency in pollutants remediation.Although there is plenty of outstanding research has been put forward continuously,most of them focuses on catalysis performance and reaction mechanisms in laboratory conditions.Realizing industrial application of photo/electrocatalytic processes is still a challenge that needs to be overcome by social demand.In this regard,this review comprehensively summarized several explorations in thefield of photo/electrocatalytic reduction towards potential industrial applications in recent years.Special attention is paid to the successful attempts and the current status of photo/electrocatalytic water splitting,carbon dioxide conversion,resource utilization from waste,etc.,by using advanced reactors.The key problems and challenges of photo/electrocatalysis in future industrial practice are also discussed,and the possible development directions are also pointed out from the industry view.
基金the financial support from the Sinopec Catalyst Co.Ltd.,China。
文摘A series of model catalysts were obtained by treating commercial fresh and spent catalysts unloaded from the factory with different methods, including green oil dipping, extraction and high-temperature regeneration;finally, the deactivation behavior of the commercial catalyst for acetylene hydrogenation were studied. The influence of various possible deactivation factors on the catalytic performance was elucidated via detailed structural characterization, surface composition analysis, and activity evaluation.The results showed that green oil, carbon deposit and sintering of active metal were the main reasons for deactivation, among which green oil and carbon deposit led to rapid deactivation, while the activity could be recovered after regeneration by high-temperature calcination. The sintering of active metal components was attributed to the high-temperature regeneration in hydrothermal conditions, which was slow but irreversible and accounted for permanent deactivation. Thus, optimizing the regeneration is expected to extend the service life of the commercial catalyst.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82173182)the Sichuan Science and Technology Program(Grant No.2021YJ0117 to Weiya Wang+1 种基金Grant No.2023NSFSC1939 to Dan Liu)the 1·3·5 project for Disciplines of Excellence–Clinical Research Incubation Project,West China Hospital,Sichuan University(Grant Nos.2019HXFH034 and ZYJC21074)。
文摘Lung cancer is the most common and fatal malignant disease worldwide and has the highest mortality rate among tumor-related causes of death.Early diagnosis and precision medicine can significantly improve the survival rate and prognosis of lung cancer patients.At present,the clinical diagnosis of lung cancer is challenging due to a lack of effective non-invasive detection methods and biomarkers,and treatment is primarily hindered by drug resistance and high tumor heterogeneity.Liquid biopsy is a method for detecting circulating biomarkers in the blood and other body fluids containing genetic information from primary tumor tissues.Bronchoalveolar lavage fluid(BALF)is a potential liquid biopsy medium that is rich in a variety of bioactive substances and cell components.BALF contains information on the key characteristics of tumors,including the tumor subtype,gene mutation type,and tumor environment,thus BALF may be used as a diagnostic supplement to lung biopsy.In this review,the current research on BALF in the diagnosis,treatment,and prognosis of lung cancer is summarized.The advantages and disadvantages of different components of BALF,including cells,cell-free DNA,extracellular vesicles,and micro RNA are introduced.In particular,the great potential of extracellular vesicles in precision diagnosis and detection of drug-resistant for lung cancer is highlighted.In addition,the performance of liquid biopsies with different body fluid sources in lung cancer detection are compared to facilitate more selective studies involving BALF,thereby promoting the application of BALF for precision medicine in lung cancer patients in the future.
基金Scientific and Technological Resources Coordination Project of Shaanxi Province,Grant/Award Number:2020PT-002,2022PT-43 and CX-PT-18Special Fund for Military Laboratory Animals,Grant/Award Number:SYDW_KY(2021)13State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers,Grant/Award Number:CBSKL2022ZZ28。
文摘The mouse genome has a high degree of homology with the human genome,and its physiological,biochemical,and developmental regulation mechanisms are similar to those of humans;therefore,mice are widely used as experimental animals.However,it is undeniable that interspecies differences between humans and mice can lead to experimental errors.The differences in the immune system have become an impor-tant factor limiting current immunological research.The application of immunodefi-cient mice provides a possible solution to these problems.By transplanting human immune cells or tissues,such as peripheral blood mononuclear cells or hematopoietic stem cells,into immunodeficient mice,a human immune system can be reconstituted in the mouse body,and the engrafted immune cells can elicit human-specific immune responses.Researchers have been actively exploring the development and differen-tiation conditions of host recipient animals and grafts in order to achieve better im-mune reconstitution.Through genetic engineering methods,immunodeficient mice can be further modified to provide a favorable developmental and differentiation microenvironment for the grafts.From initially only being able to reconstruct single T lymphocyte lineages,it is now possible to reconstruct lymphoid and myeloid cells,providing important research tools for immunology-related studies.In this review,we compare the differences in immune systems of humans and mice,describe the devel-opment history of human immune reconstitution from the perspectives of immuno-deficient mice and grafts,and discuss the latest advances in enhancing the efficiency of human immune cell reconstitution,aiming to provide important references for im-munological related researches.
文摘目的观察七氟醚和丙泊酚对危重患儿肺功能的影响。方法选择行腹部手术合并肺炎危重患儿64例,男39例,女25例,日龄1~28 d,体重2~5 kg,ASAⅢ级。采用随机数字表法将患儿分为两组:七氟醚吸入麻醉组(S组)和丙泊酚静脉麻醉组(P组),每组32例。记录插管后30 min、术毕、术后6 h的氧合指数(OI)、肺泡动脉氧分压差(PA-aO_(2))、呼吸指数(RI)、血清髓系细胞表达触发受体-1(TREM-1)、白细胞介素-8(IL-8)浓度。记录低氧血症、心律失常、严重感染、新生儿呼吸窘迫、无法纠正的低血压等围术期不良事件的发生情况。结果与插管后30 min比较,S组术毕、术后6 h OI明显升高,PA-aO_(2)和RI明显降低(P<0.05);P组术毕OI、术后6 h PA-aO_(2)明显降低(P<0.05)。与术毕比较,S组术后6 h OI明显升高,RI明显降低(P<0.05),P组术后6 h PA-aO_(2)明显降低(P<0.05)。与P组比较,术毕、术后6 h S组OI明显升高,RI明显降低,术后6 h PA-aO_(2)明显升高(P<0.05)。两组不同时点血清TREM-1浓度差异无统计学意义。与术毕比较,P组术后6 h血清IL-8浓度明显降低(P<0.05)。与P组比较,S组术毕、术后6 h血清IL-8浓度明显降低(P<0.05)。两组患儿均无围术期不良事件发生。结论与丙泊酚比较,七氟醚能够降低危重患儿血清炎性因子IL-8浓度,对危重患儿具有肺保护作用。
基金Supported by The National Natural Science Foundation of China,No.81050033the Key Projects of Sichuan Province Science and Technology Pillar Program,No.2011SZ0237+2 种基金the Science Foundation for Distinguished Young Scholars of Sichuan Province in China,No.2010JQ0039the Key Science and Technology Project of Chinese Ministry of Public Health,No.2014114the Natural Science Key Project of North Sichuan Medical College,No.CBY12-A-ZD03
文摘AIM: To determine whether the combination of platelet count(PLT) with spleen volume parameters and right liver volume(RV) measured by magnetic resonance imaging(MRI) could predict the Child-Pugh class of liver cirrhosis and esophageal varices(EV).METHODS: Two hundred and five cirrhotic patients with hepatitis B and 40 healthy volunteers underwent abdominal triphasic-enhancement MRI and laboratory examination of PLT in 109/L. Cirrhotic patients underwent endoscopy for detecting EV. Spleen maximal width(W), thickness(T) and length(L) in mm together with spleen volume(SV) and RV in mm3 were measured by MRI, and spleen volume index(SI) in mm3 was obtained by W × T × L. SV/PLT, SI/PLT and RV × PLT/SV(RVPS) were calculated and statistically analyzed to assess cirrhosis and EV.RESULTS: SV/PLT(r = 0.676) and SI/PLT(r = 0.707) increased, and PLT(r =-0.626) and RVPS(r =-0.802) decreased with the progress of Child-Pugh class(P < 0.001 for all). All parameters could determine the presence of cirrhosis, distinguish between each class of Child-Pugh class, and identify the presence of EV [the areas under the curve(AUCs) = 0.661-0.973]. A m o n g p a ra m e t e r s, R V P S c o u l d b e s t d e t e r m i n e presence and each class of cirrhosis with AUCs of 0.973 and 0.740-0.853, respectively; and SV/PLT could best identify EV with an AUC of 0.782.CONCLUSION: The combination of PLT with SV and RV could predict Child-Pugh class of liver cirrhosis and identify the presence of esophageal varices.
基金supported by the National Natural Science Foundation of China,No.81501935the Shandong Provincial Natural Science Foundation of China,No.ZR2014HQ065
文摘High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.
文摘AIM: To study the therapeutic effect of norcantharidin(NCTD) combined with ABT-737 on hepatocellular carcinoma cells and the molecular mechanism. METHODS: Two hepatocellular carcinoma(HCC) cell lines, Hep G2 and SMMC-7721, were selected. ABT-737 and NCTD were allocated into groups to be used alone or in combination. Hep G2 and SMMC-7721 cells were cultured in vitro. Liver cancer cells in the logarithmic phase of growth were vaccinated and cultured to the cell wall stage; these cells were treated for 48 h with different concentrations of NCTD, or ABT-737, or NCTD combined with ABT-737. The cell proliferation inhibition rate was detected by methyl thiazolyl tetrazolium. The expression of Mcl in HCC cells was detected by Western Blotting, and the cells in each group after treatment had apoptosis detected by flow cytometry. The proliferation inhibition rate, the expression of Mcl-1 in cells and the apoptosis inducing effect of treatment were observed in each group, and the effect of NCTD on ABT-737 in the treatment of HCC and its mechanism of action were analyzed.RESULTS: As the concentration of NCTD increased, the cell proliferation inhibition rate gradually decreased; and the treatment effect of ABT-737 1-3 μm combined with NCTD on cell proliferation inhibition was stronger than that of ABT-737 alone. The difference was statistically significant(P < 0.05). In observing the expression of Mcl-1 in cells after the treatment of different concentrations of NCTD, this was partiallyinhibited after treatment with NCTD 15 μm, and the expression of Mcl-1 was almost undetectable after treatment with NCTD 30 μm and 60 μm. The effect on inducing apoptosis with the treatment of ABT-737 or NCTD alone for 48 h was lower than that of the control group. The difference was not statistically significant(P > 0.05). The effect on inducing apoptosis in Hep G2 and SMMC-7721 cells with the treatment of ABT-737 combined with NCTD for 48 h was greater than that of ABT-737 or NCTD alone. The difference was statistically significant(P < 0.05). CONCLUSION: NCTD combined with ABT-737 has a positive role in the treatment of HCC, and it has great value in clinical research.
文摘Blindness and vision impairment are the most devastating global health problems resulting in a substantial economic and social burden.Delivery of drug to particular parts of the anterior or posterior segment has been a major challenge due to various protective barriers and elimination mechanisms associated with the unique anatomical and physiological nature of the ocular system.Drug administration to the eye by conventional delivery systems results in poor ocular bioavailability(<5%).The designing of a novel approach for a safe,simple,and effective ocular drug delivery is a major concern and requires innovative strategies to combat the problem.Over the past decades,several novel approaches involving different strategies have been developed to improve the ocular delivery system.Among these,the ophthalmic in-situ gel has attained a great attention over the past few years.This review discussed and summarized the recent and the promising research progress of in-situ gelling in ocular drug delivery system.
文摘With the increasing demand for electricity,an efficiency improvement and thereby reduced CO2 emissions of the coal-fired plants are expected in order to reach the goals set in the Kyoto protocol.It can be achieved by a rise of the process parameters.Currently,live steam pressures and temperatures up to 300 bars and 923 K are planned as the next step.Closed circuit steam cooling of blades and vanes in modern steam turbines is a promising technology in order to establish elevated live steam temperatures in future steam turbine cycles.In this paper,a steam-cooled test vane in a cascade with external hot steam flow is analyzed numerically with the in-house code CHTflow.A parametric analysis aiming to improve the cooling effectiveness is carried out by varying the cooling mass flow ratio.The results from two investigated cases show that the steam cooling technique has a good application potential in the steam turbine.The internal part of the vane is cooled homogeneously in both cases.With the increased cooling mass flow rate,there is a significant improvement of cooling efficiency at the leading edge.The results show that the increased cooling mass flow ratio can enhance the cooling effectiveness at the leading edge.With respect to trailing edge,there is no observable improvement of cooling effectiveness with the increased cooling mass flow.This implies that due to the limited dimension at the trailing edge,the thermal stress cannot be decreased by increasing the cooling mass flow rate.Therefore,impingement-cooling configuration at the trailing edge might be a solution to overcome the critical thermal stress there.It is also observed that the performance of the cooling effective differs on pressure side and suction side.It implicates that the equilibrium of the cooling effectiveness on two sides are influenced by a coupled relationship between cooling mass flow ratio and hole geometry.In future work,optimizing the hole geometry and cooling steam supply conditions might be the solutions for an equivalent cooling effectiveness along whole profile.
基金We are grateful to Xizhi Ma, Junnian Zhou, Tianhong Xu, Xu Liu, Xu Ding, Yang Liu, Ying Peng, Congwu Chi, Yiying Shang, Mingyao Ying, Sheng Ding, Lei Sun, Lei Tian, Huanhu Zhu, Hua Huang, Hongmei Li, and Xiaomo Wu for cDNA constructs and partial transgenic work, and Lihui Zhou （East China University of Science and Technology, China） for scanning electron microscopy. We thank Duc Nguyen （Yale University, USA） for critical reading and editing of this manuscript. This work is supported by grants from the National Natural Science Foundation of China （Grant Nos. 30030080, 39970408 and 30470840）, National Basic Research Program of China （973） （Grant No. 2006CB806700）.
文摘We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.
基金supported by the National Natural Science Foundation of China(Nos.51973116,U1832109,21935002,52003156)the Users with Excellence Program of Hefei Science Center CAS(2019HSC-UE003)+1 种基金the starting grant of ShanghaiTech UniversityState Key Laboratory for Modification of Chemical Fibers and Polymer Materials。
文摘Silks have various advantages compared with synthetic polymer fibers,such as sustainability,mechanical properties,luster,as well as air and humidity permeability.However,the functionalization of silks has not yet been fully developed.Functionalization techniques that retain or even improve the sustainability of silk production are required.To this end,a low-cost,effective,and scalable strategy to produce TCSs by integrating yarn-spinning and continuous dip coating technique is developed herein.TCSs with extremely long length(>10 km),high mechanical performance(strength of 443.1 MPa,toughness of 56.0 MJ m−3,comparable with natural cocoon silk),and good interfacial bonding were developed.TCSs can be automatically woven into arbitrary fabrics,which feature super-hydrophobicity as well as rapid and programmable thermochromic responses with good cyclic performance:the response speed reached to one second and remained stable after hundreds of tests.Finally,applications of TCS fabrics in temperature management and dynamic textile displays are demonstrated,confirming their application potential in smart textiles,wearable devices,flexible displays,and human–machine interfaces.Moreover,combination of the fabrication and the demonstrated applications is expected to bridge the gap between lab research and industry and accelerate the commercialization of TCSs.
