Predictive effect of lipopolysaccharide-stimulated inflammatory cytokines on symptoms of generalized anxiety disorder

BACKGROUND Generalized anxiety disorder(GAD)is a relatively common mental disorder.Recently,inflammation,an important factor for the development of depression,has attracted increasing attention.Several studies have shown that inflammatory cytokines can affect the pathophysiological processes of several nervous system diseases.We hypothesized that there is a correlation between the levels of lipopolysaccharide(LPS)-stimulated inflammatory cytokines and the clinical symptoms of GAD.AIM To investigate the predictive effect of LPS-stimulated inflammatory cytokines on symptoms of GAD.METHODS This was a cross-sectional study in which 89 patients with GAD diagnosed at The First Hospital of Hebei Medical University from January 2022 to December 2022 and 70 individuals without anxiety and depression(controls)during the same period were included.Fasting venous blood was collected from all the subjects in heparin tubes,and another 3 ml of blood was supplemented with LPS(10 ng/ml).The plasma levels of 12 cytokines[Interleukin(IL)-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,tumor necrosis factor(TNF)-α,interferon(IFN)-γ,IL-17A,IL-12p70,and IFN-α]were detected.RESULTS Post-LPS stimulation,the levels of IL-1β,IL-6,IL-8,IL-10,and TNF-αin both the control and GAD groups were significantly elevated above those in the nonstimulated groups,with IL-6 and IL-8 showing marked increases.Increases in IL-8 and TNF-αwere statistically significant in the GAD group(P<0.05).IL-1β,IL-6,IL-8,IL-10,and TNF-αwere found to be significantly correlated with Hamilton Anxiety Rating Scale(HAMA)scores(P<0.05).A negative correlation was observed between IL-10 levels and HAMA scores.Further analysis revealed that TNF-αwas associated with mental anxiety,whereas IL-1β,IL-8,and IL-10 were associated with physical anxiety symptoms,with IL-10 showing a negative correlation with physical anxiety.IL-6 was associated with both mental and physical aspects of anxiety.CONCLUSION The physical symptoms of GAD are related to inflammatory factors.IL-1β,IL-8,IL-10,and TNF-a can be used as predictors of physical or mental anxiety in patients with GAD.

Interfacial pressure distribution and bonding characteristics in twin-roll casting of Cu/Al clad strip

The mechanical properties and product thickness specifications of bimetallic clad strip prepared by twin-roll casting are tightly related to the mechanical behavior of bonding interface interaction.The thermal−flow coupled simulation and the interface pressure calculation models are established with the cast-rolling velocity as the variable.The results show that the interface temperature decreases,the interface pressure and the proportion of the thickness of the Al side increase with the decrease in cast-rolling velocity.The thinning of Cu strip mainly occurs in the backward slip zone.The higher pressure and longer solid/semi-solid contact time make the interface bonded fully,which provides favorable conditions for atomic diffusion.The inter-diffusion zone with a width of 4.9μm is attained at a cast-rolling velocity of 2.4 m/min,and the Cu side surface is nearly completely covered by aluminum.Therefore,the ductile fracture occurs on the Al side,which prevents the propagation of interface delamination cracks effectively.Meanwhile,shear effect becomes more significant at high interfacial pressure and large plastic strain,and the microstructure on Al side is composed of slender columnar crystals.Thus,the metallurgical bonding and refinement of grains on the Al side can result in higher bonding strength and tensile properties of the clad strip.

Effect of hot rolling and cooling process on microstructure and properties of 2205/Q235 clad plate

The 2205/Q235 clad plate was fabricated by vacuum hot rolling with symmetrical assembling pattern of Q235/2205/2205/ Q235.The flow stress behavior and processing map of 2205 duplex stainless steel (DSS)were investigated by hot compressive tests on a Gleeble-3800 simulator.Then,thermal-mechanical coupled nonlinear finite element models of vacuum hot rolling and subsequent cooling process were established.From the simulation results,the influence of rolling reduction and rolling speed on hot deformation state of 2205 DSS in the assembled slab was disclosed and the optimal rolling parameters were presented.Meanwhile,the cooling rate of 2205 DSS under different cooling conditions and thicknesses of the clad plate was obtained.According to the numerical simulation results,pilot experiments were successfully carried out on a laboratory scale.The material universal testing machine,optical microscope,scanning electron microscope and energy-dispersive spectrometer were used to evaluate the mechanical properties and microstructure of bonding interface and 2205 DSS matrix for different rolling reduction and cooling processes.The results showed that with symmetrical assembling pattern,the approximate thermodynamic conditions can be established for 2205 DSS to avoid cracks in hot rolling process.When the rolling reduction increased from 10 to 40%,the shear strength of the bonding interface is increased from 120 to 530 MPa,and the uniform two-phase microstructure of 2205 DSS and satisfactory' mechanical properties can be obtained with cooling rate higher than 10℃/s between 1050 and 500℃ after rolling.

Analysis of longitude profiled rolling process of Cu/Al cladded sheet and evaluation of outlet warpage

Longitude profiled cladded sheet is obtained by rolling bimetal cladded sheet with variable gauge rolling technology.The longitude profiled rolling process of Cu/Al cladded sheets was studied by finite element method and experiments.The rolling force rises with the increase in reduction,and a sudden change appears at the end of the thickness variation zone.The thickness ratio of copper layer is enlarged after rolling owing to its relatively large deformation resistance and continues to rise with the increase in reduction rate.Lower elongation of copper side leads to the warpage of exit metal to copper side,which further hinders the deformation of copper.The influence of asymmetric rolling parameters indicates that increasing the work roll diameter ratio,speed radio,and friction coefficient on Al side can reduce the warpage when the reduction rate is below a certain value depending on the thickness ratio and other rolling parameters.Comparison of experiments and simulation results showed good agreement and verified the finite element model.

Analysis of coupling mechanism between roll system crossing and liner wear of hot strip mill

Non-design roll system crossing seriously affects the plate shape and rolling mill performance.The problem of roll system crossing caused by liner wear was studied.The finite element model of rolling mill was established to analyze the relationship between roll system crossing and liner wear.The wear of liner was measured by laser tracker.The range of roll system crossing angle was calculated by considering the amount of stand clearance obtained by numerical simulation.The wear surface morphology of liner was observed and the wear mechanism was analyzed.The liner wear experiment was carried out to analyze the wear amount of the liner.Finally,based on the Archard wear theory,the prediction model of the cross angle of the roll system and the wear amount of the liner was established.Because there are more uncertain factors in the field production,the prediction model cannot be considered one by one.Therefore,the predicted value is smaller than the actual wear value,but it still has great reference.