Insight into the spoilage heterogeneity of meat-borne bacteria isolates with high-producing collagenase

Chilled chicken is inevitably contaminated by microorganisms during slaughtering and processing,resulting in spoilage.Cutting parts of chilled chicken,especially wings,feet,and other skin-on products,are abundant in collagen,which may be the primary target for degradation by spoilage microorganisms.In this work,a total of 17 isolates of spoilage bacteria that could secrete both collagenase and lipase were determined by raw-chicken juice agar(RJA)method,and the results showed that 7 strains of Serratia,Aeromonas,and Pseudomonas could significantly decompose the collagen ingredients.The gelatin zymography showed that Serratia liquefaciens(F5)and(G7)had apparent degradation bands around 50 kDa,and Aeromonas veronii(G8)and Aeromonas salmonicida(H8)had a band around.65 and 95 kDa,respectively.The lipase and collagenase activities were detected isolate-by-isolate,with F5 showing the highest collagenase activity.For spoilage ability on meat in situ,F5 performed strongest in spoilage ability,indicated by the total viable counts,total volatile basic nitrogen content,sensory scores,lipase,and collagenase activity.This study provides a theoretical basis for spoilage heterogeneity of strains with high-producing collagenase in meat.

Full-Scale Isogeometric Topology Optimization of Cellular Structures Based on Kirchhoff-Love Shells

Cellular thin-shell structures are widely applied in ultralightweight designs due to their high bearing capacity and strength-to-weight ratio.In this paper,a full-scale isogeometric topology optimization(ITO)method based on Kirchhoff-Love shells for designing cellular tshin-shell structures with excellent damage tolerance ability is proposed.This method utilizes high-order continuous nonuniform rational B-splines(NURBS)as basis functions for Kirchhoff-Love shell elements.The geometric and analysis models of thin shells are unified by isogeometric analysis(IGA)to avoid geometric approximation error and improve computational accuracy.The topological configurations of thin-shell structures are described by constructing the effective density field on the controlmesh.Local volume constraints are imposed in the proximity of each control point to obtain bone-like cellular structures.To facilitate numerical implementation,the p-norm function is used to aggregate local volume constraints into an equivalent global constraint.Several numerical examples are provided to demonstrate the effectiveness of the proposed method.After simulation and comparative analysis,the results indicate that the cellular thin-shell structures optimized by the proposed method exhibit great load-carrying behavior and high damage robustness.

Experimental Study on the Ultrasonic Testing including Porosity of Rock Damage Characteristics

Cement mortar specimens are used to simulate the fracture of rock material under uniaxial compression test, using the ultrasonic instrument measurement test process, the waveform and wave velocity results along with the change of specimen fracture extension are obtained. Experimental results show that at 0 MPa, there are micro-defects in the specimen, leading to the irregular change of the waveform of the measuring points 4# and 5#;At 1 - 4 MPa, there is no crack on the surface of the specimen, the waveform of ultrasonic wave is stable, and the velocity of each point increases with the increase of the strain, but the measuring point increases the wave velocity when the load is applied;When the specimen surface cracks, expect the waveform of measuring point 4# and 5# change in irregularities, the measuring point 2# waveform also changes irregularly and measuring point of the wave velocity has decreased;At the crack propagation stage, ultrasonic wave also changes irregularly along with the expansion of crack, wave velocity with the increase of the strain is also obvious downward trend;When the crack penetrates the whole specimen, the waveform of each measuring point changes abnormally, the continuity is bad, the waveform is irregular and the measured wave velocity is low.

Lithium polysulfide solvation and speciation in the aprotic lithium-sulfur batteries

Fingerprinting sulfur speciation in aprotic electrolytes is a key to understand fundamental chemistry and design well-performing lithium–sulfur(Li–S)batteries.Lithium polysulfide(LiPS)dissolution and deposition in ether-based electrolytes during redox reactions have been probed and established by spectroscopy and microscopy.However,detailed LiPS structure and solvation properties influenced by conventional and newly developed electrolytes remain elusive,which exert fundamental challenges and practical difficulties in decoupling battery performance from electrolyte volume.This perspective aims to provide timely information to uncover underlying mechanisms that rein in sulfur speciation by considering the charge density of LiPSs and the coordination strength of solvents/salts.The discussion starts with unlocking the baseline electrolyte formulation to investigate its role in LiPS formation and compatibility.After that,the term coordination strength is used instead of donor number and dielectric constant to describe interactions between solvents and LiPSs and to reveal LiPS structure evolution.This work is expected to encourage the discovery of new electrolyte working mechanisms to develop energy-dense and power-intensive Li–S batteries.

Catalytic thermal decomposition of ammonium perchlorate by a series of lanthanide EMOFs

Given their unique and excellent properties,metal-organic frameworks(MOFs)materials have been used in many scientific fields.EMOFs use energetic materials as ligands,which can provide part of the energy for the system while catalyzing ammonium perchlorate.The energetic material 1.1'-dihydroxyazotetrazole(H_(2)AzTO),as a high-energy nitrogen-rich material,was selected as a ligand.Five kinds of La^(3+),Ce^(3+),Pr^(3+),Nd^(3+),and Sm^(3+)lanthanide EMOFs were synthesized and obtained.Single crystal X-ray diffraction tests were conducted to obtain the crystal structures of EMOFs 1-5,which indicate that they have similar crystal structures.The thermal stabilities of EMOFs 1-5,which are obtained by differential scanning calorimetry(DSC)tests,are improved compa red with that of the ligand.The results of thermicdecomposition of ammonium perchlorate(AP)and AP mixtures with 10 wt%EMOFs 1-5 show that except for AP mixed with 10 wt%co mpound 2,the high-temperature decomposition peak tempe rature of AP mixed with other compounds is significantly advanced(up to 59.3-88.3 K),and the decomposition of AP is continuous and violent.EMOFs 3-5 have good application prospects for the catalytic thermicdecomposition of AP.

Freeze-drying assisted liquid exfoliation of BiFeO_(3) for pressure sensing

Breaking up bulk crystals of functional materials into nanoscale thinner layers can lead to interesting properties and enhanced functionalities due to the size and interface effects.However,unlike the van der Waals layered crystals,many materials cannot be exfoliated into thin layers by liquid exfoliation.BiFeO_(3) is a piezoelectric ceramic material,which is commonly synthesized as bulk crystals,limiting its wider applications.In this contribution,a freeze-drying assisted liquid exfoliation method was adopted to fabricate thin-layered BiFeO_(3) nanoplates with lateral sizes of up to 500 nm and thicknesses of 10−20 nm.The freeze-drying process showed a vital role in the preparation process by imposing stress on the dispersed BiFeO_(3) crystals during the liquid-to-solid-to-gas transition of the solvent.Such stress resulted in lattice strains in the freeze-dried BiFeO_(3) crystals,which enabled their further exfoliation under subsequent ultrasonication.Considering the intrinsic piezoelectric effect of BiFeO_(3),pressure sensors based on bulk and thin-layer BiFeO_(3) were also fabricated.The pressure sensor based on BiFeO_(3) nanoplates exhibited a largely enhanced sensitivity with a wider working range than the bulk counterpart,because of the stronger piezoelectric effect induced and the extra electrical charges at abundant interlayer interfaces.We suggest that the freeze-drying assisted liquid exfoliation method can be applied to other non-van der Waals crystals to bring about more functional material systems.

Small-signal stability analysis of photovoltaic generation connected to weak AC grid

A small-signal model of photovoltaic(PV)generation connected to weak AC grid is established based on a detailed model of the structure and connection of a PV generation system. An eigenvalue analysis is then employed to study the stability of PV generation for different grid strengths and control parameters in a phaselocked loop(PLL) controller in the voltage source converter. The transfer function of the power control loop in the dq rotation frame is developed to reveal the influence mechanism of PLL gains on the small-signal stability of PV generation. The results can be summarized as follows:(1)oscillation phenomena at a frequency of about 5 Hz may occur when the grid strength is low;(2) the tuning control parameters of the PLL have a noticeable effect on the damping characteristics of the system, and larger proportional gain can improve the system damping;(3)within a frequency range of 4-5 Hz,the PLL controller has positive feedback on the power loop of PV generation. A virtual inductance control strategy is proposed to improve the operational stability of PV generation. Finally, a simulation model of PV generation connected to weak AC grid is built in PSCAD/EMTDC and the simulation results are used to validate the analysis.

Rare-earth,nitrogen-rich,oxygen heterocyclic supramolecular compounds(Nd,Sm,and Eu):Synthesis,structure,and catalysis for ammonium perchlorate

Three novel rare-earth,nitrogen-rich and oxygen heterocyclic supramolecular complexes,namely[Nd(BTF)_(2)(H_(2)O)_(5)]_(n),[Sm(BTF)_(2)(H_(2)O)_(5)]_(n),and[Eu(BTF)_(2)(H_(2)O)_(5)]_(n),were synthesized.A single crystal was obtained by the solvent evaporation method,and the structure and coordination mode of metal complexes were determined by single crystal X-ray diffraction.Results show that the supramolecular complexes contain many hydrogen bonds and thus have good thermal stability(T_(dec)>540 K).The thermal decomposition of ammonium perchlorate(AP)catalyzed by the complexes was investigated by differential thermal analysis,which reveals a pre-eminent catalytic effect on AP.The high temperature decomposition peak of AP can be advanced by nearly 90 K at the amount of added complexes of 10 wt%,and the activation energy of AP descent range is from 70 to 150 kJ/mol.The other properties were fully characterized through elemental analysis and Fourier transform infrared spectroscopy.

Ultra-broadband edge-state pair for zigzag-interfaced valley Hall insulators

The existence of a single topologically protected edge state in the first bulk bandgap for acoustic/elastic valley Hall insulators(VHIs)with zigzag interface configurations(ZICs)is well known.However,in this work,we show that an ultra-broadband edgestate pair in this bandgap can be created using the inverse design by topology optimization.The valley Hall insulator design increases the operational bandwidth 121%compared with an existing valley Hall insulator from recent literature and exhibits extreme field confinement,where more than 99%of the field intensity is concentrated within three unit-cells from the interface.One-way propagation and topological robustness towards small cavity defects are confirmed for the full bandwidth.The exploitation of such edge-state pairs of valley Hall insulators opens an avenue for realizing broadband confined edge modes.In tests for disorder and bend defects,we show that the additional ZIC,with a different operational frequency interval,encountered at the defects,degrades the transmission for bend and disorder defects which may prove significant for the application of VHIs.Through an alternative topology optimization method based on two ZICs,we further increase their common operational bandwidth.

Effects of submerged synthetic jet on the coherent structures in turbulent boundary layer

The research focuses on the active control scheme of a fully-developed turbulent boundary layer(TBL)forced by the submerged synthetic jet,and the particle image velocimetry(PIV)system is used to capture the two components velocity signals in the flow field.The mean velocity profiles near the wall obtained by the single-pixel ensemble correlation(SPEC)algorithm evidence the decline of streamwise velocity gradient,indicating the reduction of skin-friction drag.The deficit of streamwise velocity as well as turbulence attenuation in the cross flow attests that the perturbation effects are persistent in a certain range downstream of the synthetic jet injection section.The spatial topologies of the coherent structures detected by the improved quadrant splitting method(IQSM)reveal that the sweep events,responsible for the significant wall friction,are repressed under the action of synthetic jet.By employing the method of two-point correlation,destruction of the near-wall streaks is observed,and the control mechanism acts in a way to suppress the extension along the streamwise direction of the correlation coefficient.Based on the linear stochastic estimation,it is inferred that the influence of force is such the near-wall hairpin vortex detaches from the wall,and its ability to induce environmental fluid is vastly weakened.