BACKGROUND There are relatively few studies on continuing care of coronary heart disease(CHD),and its research value needs to be further clarified.AIM To investigate the effect of continuous nursing on treatment compl...BACKGROUND There are relatively few studies on continuing care of coronary heart disease(CHD),and its research value needs to be further clarified.AIM To investigate the effect of continuous nursing on treatment compliance and side effect management in patients with CHD.METHODS This is a retrospective study with patients from January 2021 to 2023.The study was divided into two groups with 30 participants in each group.Self-rating anxiety scale(SAS)and Self-rating depression scale(SDS)were used to assess patients'anxiety and depression,and medical coping questionnaire was used to assess patients'coping styles.The pelvic floor dysfunction questionnaire(PFDI-20)was used to assess the status of pelvic floor function,including bladder symptoms,intestinal symptoms,and pelvic symptoms.RESULTS SAS score decreased from 57.33±3.01before treatment to 41.33±3.42 after treatment,SDS score decreased from 50.40±1.45 to 39.47±1.57.The decrease of these two indexes was statistically significant(P<0.05).PFDI-20 scores decreased from the mean 16.83±1.72 before treatment to 10.47±1.3the mean after treatment,which was statistically significant(P<0.05).CONCLUSION The results of this study indicate that pioneering research in continuous care of CHD has a positive impact on improving patients'treatment compliance,reducing anxiety and depression levels,and improving coping styles and pelvic floor functional status.展开更多
The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome edit...The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.展开更多
This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenz...This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.展开更多
<strong>Purpose:</strong> Epidemiological studies have assessed the association between Caveolin-1 polymorphism and cancer risk. However, published data are still inconclusive. To clarify this inconsistenc...<strong>Purpose:</strong> Epidemiological studies have assessed the association between Caveolin-1 polymorphism and cancer risk. However, published data are still inconclusive. To clarify this inconsistency, we conducted a meta-analysis to evaluate the relationship between Caveolin-1 polymorphism (rs7804372) and cancer susceptibility. <strong>Methods:</strong> We conducted a comprehensive literature search, using PubMed, Embase, Medline, Web of Science, CNKI, and Wanfang database, which included English and Chinese literatures. The latest updated date was January 2018. The following search terms were performed to retrieve the relevant articles: ((CAV1) OR (Caveolin-1) OR (rs7804372)) AND (cancer OR tumor OR carcinoma OR neoplasms OR malignancy) AND (polymorphism OR mutation OR variant OR genotype). Odds ratio (OR) with 95% confidence interval (CI) was used to estimate the pooled effect. <strong>Results:</strong> In the overall analysis, this kind of polymorphism showed a significant association with increased risk of cancer: allelic model (T/A;OR = 1.33, 95% CI: 1.19 - 1.49;P < 0.0001), homozygous (TT/AA;OR = 1.73, 95% CI: 1.37 - 2.18;P < 0.0001), and heterozygous genetic models (TT/TA;OR = 1.23, 95% CI: 1.14 - 1.33;P < 0.0001), the dominant genetic model (TT + TA/AA;OR = 1.58, 95% CI: 1.28 - 1.96;P < 0.0001), and the recessive genetic model (TT/TA + AA;OR = 1.34, 95% CI: 1.20 - 1.50;P < 0.0001). In addition, the stratified analysis of the results was carried out by ethnicity, HWE status, and cancer types. The outcome indicated that Caveolin-1 rs7804372 polymorphism was associated with an increased risk of cancer. <strong>Conclusion:</strong> The present study demonstrated that the allele T of Caveolin-1 (rs7804372) polymorphism might associate with increased susceptibility to cancer, and might predict worse survival in patients with various types of cancer. However, further well-designed studies are required to evaluate this association.展开更多
Tracking moving wideband sound sources is one of the most challenging issues in the acoustic array signal processing which is based on the direction of arrival(DOA) estimation. Compressive sensing(CS) is a recent theo...Tracking moving wideband sound sources is one of the most challenging issues in the acoustic array signal processing which is based on the direction of arrival(DOA) estimation. Compressive sensing(CS) is a recent theory exploring the signal sparsity representation, which has been proved to be superior for the DOA estimation. However, the spatial aliasing and the offset at endfire are the main obstacles for CS applied in the wideband DOA estimation. We propose a particle filter based compressive sensing method for tracking moving wideband sound sources. First, the initial DOA estimates are obtained by wideband CS algorithms. Then, the real sources are approximated by a set of particles with different weights assigned. The kernel density estimator is used as the likelihood function of particle filter. We present the results for both uniform and random linear array. Simulation results show that the spatial aliasing is disappeared and the offset at endfire is reduced. We show that the proposed method can achieve satisfactory tracking performance regardless of using uniform or random linear array.展开更多
Motion accuracy of space manipulators has direct effects on the ability of the systems to perform specified tasks. However, some design variables are inherently interval parameters due to uncertainties in geometric st...Motion accuracy of space manipulators has direct effects on the ability of the systems to perform specified tasks. However, some design variables are inherently interval parameters due to uncertainties in geometric structures, material properties, and so on. This paper presents Chebyshev inclusion function(CIF) for approximating the dynamic responses function of parametrically excited systems. Motion accuracy reliability(MAR) of space manipulators was evaluated based on mechanism reliability analysis methods and interval uncertainty model. To illustrate the accuracy of the proposed method, a two-link manipulator with interval parameters was demonstrated. The results showed that the proposed method required much fewer samples to obtain more accurate reliability compared with the traditional Monte Carlo simulation(MCS). Finally, the sensitivity analysis was performed to facilitate the optimization design by using global sensitivity analysis.展开更多
Wideband acoustic imaging,which combines compressed sensing(CS)and microphone arrays,is widely used for locating acoustic sources.However,the location results of this method are unstable,and the computational efficien...Wideband acoustic imaging,which combines compressed sensing(CS)and microphone arrays,is widely used for locating acoustic sources.However,the location results of this method are unstable,and the computational efficiency is low.In this work,in order to improve the robustness and reduce the computational cost,a DCS-SOMP-SVD compressed sensing method,which combines the distributed compressed sensing using simultaneously orthogonal matching pursuit(DCS-SOMP)and singular value decomposition(SVD)is proposed.The performance of the DCS-SOMP-SVD is studied through both simulation and experiment.In the simulation,the locating results of the DCS-SOMP-SVD method are compared with the wideband BP method and the DCS-SOMP method.In terms of computational efficiency,the proposed method is as efficient as the DCS-SOMP method and more efficient than the wideband BP method.In terms of locating accuracy,the proposed method can still locate all sources when the signal to noise ratio(SNR)is−20 dB,while the wideband BP method and the DCS-SOMP method can only locate all sources when the SNR is higher than 0 dB.The performance of the proposed method can be improved by expanding the frequency range.Moreover,there is no extra source in the maps of the proposed method,even though the target sparsity is overestimated.Finally,a gas leak experiment is conducted to verify the feasibility of the DCS-SOMP-SVD method in the practical engineering environment.The experimental results show that the proposed method can locate both two leak sources in different frequency ranges.This research proposes a DCS-SOMP-SVD method which has sufficient robustness and low computational cost for wideband acoustic imaging.展开更多
BACKGROUND Venous thromboembolism(VTE)is a major cause of unexpected and perioperative in-hospital deaths.It is characterized by high morbidity,high mortality,high misdiagnosis rate,and high missed diagnosis rates.VTE...BACKGROUND Venous thromboembolism(VTE)is a major cause of unexpected and perioperative in-hospital deaths.It is characterized by high morbidity,high mortality,high misdiagnosis rate,and high missed diagnosis rates.VTE is a common postoperative complication in cancer patients.VTE is preventable,and early identification of risk factors leading to VTE and appropriate early preventive actions can reduce its occurrence and mortality.Presently,there is no uniform standard for the prevention and control of VTE in clinical practice,and hospitals in China lack mature and effective protocols for the assessment,prevention,and treatment of VTE.AIM To explore whether an early warning program could influence the occurrence of deep vein thrombosis(DVT)postoperatively.METHODS This is a comparative retrospective cohort study,which enrolled patients who underwent laparotomic or laparoscopic gastrointestinal tumor resection for gastrointestinal cancer between January 2016 and December 2019.Patients were divided into a control group and an early warning group depending on whether or not the early warning program was implemented.A venous thromboembolism prevention and control team was established.The outcomes included the occurrence of DVT,the correct rate of VTE assessment,the coagulation indicators,and the mastery of VTE knowledge by the nurses.RESULTS A total of 264 patients were included in this study,with 128 patients in the control group and 136 patients in the early warning group.The occurrence rate of DVT in the early warning group was 6.6%(9/136),compared with 14.1%(18/128)in the control group(P<0.05).The correct rates of VTE risk assessment by the nurses and standard implementation rate of VTE preventive measures were 86.8%vs 65.6%and 80.2%vs 57.8%in early warning and control groups,respectively(all P<0.001).The independent factors associated with postoperative DVT occurrence were age(OR=1.083,95%CI:1.070-3.265,P=0.032),Hyperlipidemia(OR=1.127,95%CI:1.139-2.564,P=0.042),preoperative high VTE risk(OR=2.131,95%CI:1.085-5.178,P=0.001),time of operation(OR=2.268,95%CI:2.005-5.546,P=0.026)and not adoption of early warning prevention(OR=3.747,95%CI:1.523-6.956,P=0.017).CONCLUSION The early warning strategy was independently associated with the decreasing occurrence of VTE,and it might be suitable for protection from VTE in patients undergoing gastrointestinal cancer surgery.展开更多
In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still n...In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.展开更多
Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were ...Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were randomly divided into five groups,A:H9C2 cell group B:H9C2 cells+H2O2 model group C:H9C2 cells+H2O2 model+Qishen Yiqi group D:H9C2 cells+H2O2 model+Qishen Yiqi+wort group E:H9C2 cells+H2O2 model+Qishenyiqi+5-HD group,the drug intervention is according to the corresponding conditions.CCK-8 method was used to detect the cell activity of each group;Western blot was used to detect the expression of AKT and P-Akt proteins in myocardial cells in each group.The current was recorded by the standard patch clamp whole cell recording method,and the current was collected and analyzed by Pclamp6.0 software.Results:CCK-8 test results showed that compared with group A,the activity of myocardial cells in group B was significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,the difference in group C was statistically significant(P<0.01);compared with C,cardiomyocyte activity in D and E group were significantly decreased,and the difference was statistically significant(P<0.05);WB results showed that compared with A,p-Akt protein expression in B,C,D and E groups were significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,p-Akt protein expression in C,D and E group were significantly increased,and the difference was statistically significant(P<0.01),but there was no significant difference in AKT expression among groups(P>0.05);The results of whole cell patch clamp experiment showed that the outward current of B was significantly increased compared with that of A,and the difference between groups was statistically significant(P<0.01);compared with group B,cardiomyocytes in group C further increased the outward current,and the difference between groups was statistically significant(P<0.01);compared with C,the current of D and E group were significantly decreased,with statistical significance between groups(P<0.01).Conclusion:QishenYiqi dropping pills can protect cardiomyocytes by activating p-Akt protein expression and KATP channel opening in H9C2 cardiomyocytes.展开更多
MicroRNAs (miRNAs) are pivotal modulators of plant development and host-virus interactions. However, the roles and action modes of specific miRNAs involved in viral infection and host susceptibility remain largely u...MicroRNAs (miRNAs) are pivotal modulators of plant development and host-virus interactions. However, the roles and action modes of specific miRNAs involved in viral infection and host susceptibility remain largely unclear. In this study, we show that Rice ragged stunt virus (RRSV) infection caused increased accumulation of miR319 but decreased expression of miR319-regulated TCP (TEOSINTE BRANCHED/ CYCLOIDEA/PCF) genes, especially TCP21, in rice plants. Transgenic rice plants overexpressing miP,319 or downregulating TCP21 exhibited disease-like phenotypes and showed significantly higher susceptibility to RRSV in comparison with the wild-type plants. In contrast, only mild disease symptoms were observed in RRSV-infected lines overexpressing TCP21 and especially in the transgenic plants overexpressing miR319- resistant TCP21. Both RRSV infection and overexpression of miR319 caused the decreased endogenous jasmonic acid (JA) levels along with downregulated expression of JA biosynthesis and signaling-related genes in rice. However, treatment of rice plants with methyl jasmonate alleviated disease symptoms caused by RRSV and reduced virus accumulation. Taken together, our results suggest that the induction of miR319 by RRSV infection in rice suppresses JA-mediated defense to facilitate virus infection and symp- tom development.展开更多
Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not ...Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants.In this study,we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes(ATGs).We showed that depletion of the ELONGATED HYPOCOTYL 5(HY5),a key component of light signaling,leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments,contributing to higher expression oiATGs.HY5 interacts with and recruits HISTONE DEACETYLASE 9(HDA9)to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3.Furthermore,we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci,leading to their depression and thereby activated autophagy.Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway.Collectively,our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants.展开更多
In this study, we aimed to investigate the functional network changes that occur in patients with lower back pain(LBP). We also investigated the link between LBP and the small-world properties of functional networks w...In this study, we aimed to investigate the functional network changes that occur in patients with lower back pain(LBP). We also investigated the link between LBP and the small-world properties of functional networks within the brain. Functional MRI(fMRI) was performed on 20 individuals with LBP and 17 age and gender-matched normal controls during the resting state. The severity of the pain in the individuals with LBP ranged from 5 to 8 on a 0–10 scale, with 0 indicating no pain. Network-based statistics were performed to investigate the differences between the brain networks of individuals with LBP and those of normal controls. Several small-world parameters of brain networks were calculated, including the clustering coefficient, characteristic path length, local efficiency, and global efficiency. These criteria reflect the overall network efficiency. The brain networks in the individuals with LBP due to herniation of a lumbar disc demonstrated a significantly longer characteristic path length as well as a lower clustering coefficient, global efficiency, and local efficiency compared to those in control subjects. We found that LBP patients tended to have unstable and inefficient brain networks when compared with healthy controls. In addition, LBP individuals showed significantly decreased functional connectivity in the anterior cingulate cortex, middle cingulate cortex, post cingulate cortex, inferior frontal gyrus, middle temporal gyrus, occipital gyrus, postcentral gyrus, precentral gyrus, supplementary motor area, thalamus, fusiform, caudate, and cerebellum. We believe that these regions may be involved in the pathophysiology of lower back pain.展开更多
We propose and demonstrate an agile X-band signal synthesizer with ultralow phase noise based on all-fiberphotonic techniques for radar applications. It shows phase noise of-145 dBc∕Hz(-152 dBc∕Hz) at 10 kHz(100 kHz...We propose and demonstrate an agile X-band signal synthesizer with ultralow phase noise based on all-fiberphotonic techniques for radar applications. It shows phase noise of-145 dBc∕Hz(-152 dBc∕Hz) at 10 kHz(100 kHz) offset frequency for 10 GHz carrier frequency with integrated RMS timing jitter between 7.6 and 9.1 fs(integration bandwidth: 10 Hz–10 MHz) for frequencies from 9 to 11 GHz. Its frequency switching time is evaluated to be 135 ns with a 135 pHz frequency tuning resolution. In addition, the X-band linearfrequency-modulated signal generated by the proposed synthesizer shows a good pulse compression ratio approximating the theoretical value. In addition to the ultrastable X-band signals, the proposed synthesizer can also provide 0–1 GHz ultralow-jitter clocks for analog-to-digital converters(ADC) and digital-to-analog converters(DAC) in radar systems and ultralow-jitter optical pulse trains for photonic ADC in photonic radar systems.The proposed X-band synthesizer shows great performance in phase stability, switching speed, and modulation capability with robustness and potential low cost, which is enabled by an all-fiber-photonics platform and can be a compelling technology suitable for future X-band radars.展开更多
Materials that exhibit visible luminescence upon X-ray irradiation show great potential in the medical and industrial fields.Pure organic materials have recently emerged as promising scintillators for X-ray detection ...Materials that exhibit visible luminescence upon X-ray irradiation show great potential in the medical and industrial fields.Pure organic materials have recently emerged as promising scintillators for X-ray detection and radiography,due to their diversified design,low cost,and facile preparation.However,recent progress in efficient radioluminescence has mainly focused on small molecules,which are inevitably associated with processability and repeatability issues.Here,a concise strategy is proposed to prepare radioluminescent polymers that exhibit multiple emission colors from blue to yellow with high brightness in an amorphous state by the radical copolymerization of negatively charged polyacrylic acid and different positively charged quaternary phosphonium salts.One of the obtained polymers exhibits excellent photostability under a high X-ray irradiation dosage of 27.35 Gy and has a detection limit of 149 nGy s^(−1).This performance is superior to that of conventional anthracene-based scintillators.Furthermore,by simply drop-casting a polymer methanol solution on a quartz plate,a transparent scintillator screen was successfully fabricated for X-ray imaging with a resolution of 8.7 line pairs mm^(−1).The pure organic phosphorescent polymers with a highly efficient radioluminescence were demonstrated for the first time,and the strategy reported herein offers a promising pathway to expand the application range of amorphous organic scintillators.展开更多
(Molecular Plant 13,515-531;March 2020)After publication of our original manuscript,we became aware of errors in Figure 4.During the preparation of Figure 4C in this article as originally published,we inadvertently du...(Molecular Plant 13,515-531;March 2020)After publication of our original manuscript,we became aware of errors in Figure 4.During the preparation of Figure 4C in this article as originally published,we inadvertently duplicated the image of hda9-1(MS-N)as that of the hda9-1-C(MS+N).Also,in Figure 4H,the image of pUBQ10:GFP-ATG8a/hda9-1(MS+L)was mistakenly a duplicate of pUBQ10:GFP-ATG8a/WT(MS+L)shown in Figure S7C.A corrected version of Figure 4 is shown below.The scientific conclusions of this article have not been affected by this correction.The authors apologize for not detecting this error prior to publication and for any inconvenience that may have been caused.展开更多
Hippophae rhamnoides ssp.sinensis(Rha)and H.tibetana(Tib)are two species of sea buckthorn which distribute in Qinghai-Tibet Plateau.Our results showed that both sea buckthorn leaf exhibited high total phenolic and fla...Hippophae rhamnoides ssp.sinensis(Rha)and H.tibetana(Tib)are two species of sea buckthorn which distribute in Qinghai-Tibet Plateau.Our results showed that both sea buckthorn leaf exhibited high total phenolic and flavonoid content,and Rha leaf showed much higher level.In addition,66 phenolic compounds were detected,belonging to classes of benzoic acid derivatives,flavonols,flavanols,flavanones,and so on.Classes of benzoic acid derivatives and flavonols were most abundant,among them ellagic acid and rutin were the most and second abundant phenols.Besides,methyl gallate,2,6-dihydroxybenzoic acid,4-hydroxybenzoic acid,gentisic acid,narcissin,astragalin,nicotiflorin,prunin,procyanidin B3,cyanidin 3-O-rutinoside chloride,delphinidin 3-glucoside,gallocatechin,taxifolin,aromadendrin and trans-3,3′,4′,5,5′,7-Hexahydroxyflavanone were the first time to be identified in SBT leaf.Furthermore,significant free radical scavenging effects of ABTS and DPPH,ferric reducing antioxidant ability,cellular antioxidant properties and anti-proliferation activity against HepG2 cells were observed in the aqueous ethanol extracts of sea buckthorn leaf.While Rha showed stronger inhibitory effects,which might due to the higher content of total phenolic and flavonoid profiles.Our finding clearly supported that Rha leaf as a wild and non-pollution plant could be a better choice as a healthy food resource.展开更多
文摘BACKGROUND There are relatively few studies on continuing care of coronary heart disease(CHD),and its research value needs to be further clarified.AIM To investigate the effect of continuous nursing on treatment compliance and side effect management in patients with CHD.METHODS This is a retrospective study with patients from January 2021 to 2023.The study was divided into two groups with 30 participants in each group.Self-rating anxiety scale(SAS)and Self-rating depression scale(SDS)were used to assess patients'anxiety and depression,and medical coping questionnaire was used to assess patients'coping styles.The pelvic floor dysfunction questionnaire(PFDI-20)was used to assess the status of pelvic floor function,including bladder symptoms,intestinal symptoms,and pelvic symptoms.RESULTS SAS score decreased from 57.33±3.01before treatment to 41.33±3.42 after treatment,SDS score decreased from 50.40±1.45 to 39.47±1.57.The decrease of these two indexes was statistically significant(P<0.05).PFDI-20 scores decreased from the mean 16.83±1.72 before treatment to 10.47±1.3the mean after treatment,which was statistically significant(P<0.05).CONCLUSION The results of this study indicate that pioneering research in continuous care of CHD has a positive impact on improving patients'treatment compliance,reducing anxiety and depression levels,and improving coping styles and pelvic floor functional status.
文摘The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.
文摘This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.
文摘<strong>Purpose:</strong> Epidemiological studies have assessed the association between Caveolin-1 polymorphism and cancer risk. However, published data are still inconclusive. To clarify this inconsistency, we conducted a meta-analysis to evaluate the relationship between Caveolin-1 polymorphism (rs7804372) and cancer susceptibility. <strong>Methods:</strong> We conducted a comprehensive literature search, using PubMed, Embase, Medline, Web of Science, CNKI, and Wanfang database, which included English and Chinese literatures. The latest updated date was January 2018. The following search terms were performed to retrieve the relevant articles: ((CAV1) OR (Caveolin-1) OR (rs7804372)) AND (cancer OR tumor OR carcinoma OR neoplasms OR malignancy) AND (polymorphism OR mutation OR variant OR genotype). Odds ratio (OR) with 95% confidence interval (CI) was used to estimate the pooled effect. <strong>Results:</strong> In the overall analysis, this kind of polymorphism showed a significant association with increased risk of cancer: allelic model (T/A;OR = 1.33, 95% CI: 1.19 - 1.49;P < 0.0001), homozygous (TT/AA;OR = 1.73, 95% CI: 1.37 - 2.18;P < 0.0001), and heterozygous genetic models (TT/TA;OR = 1.23, 95% CI: 1.14 - 1.33;P < 0.0001), the dominant genetic model (TT + TA/AA;OR = 1.58, 95% CI: 1.28 - 1.96;P < 0.0001), and the recessive genetic model (TT/TA + AA;OR = 1.34, 95% CI: 1.20 - 1.50;P < 0.0001). In addition, the stratified analysis of the results was carried out by ethnicity, HWE status, and cancer types. The outcome indicated that Caveolin-1 rs7804372 polymorphism was associated with an increased risk of cancer. <strong>Conclusion:</strong> The present study demonstrated that the allele T of Caveolin-1 (rs7804372) polymorphism might associate with increased susceptibility to cancer, and might predict worse survival in patients with various types of cancer. However, further well-designed studies are required to evaluate this association.
基金supported by the NFSC Grants 51375385 and 51675425Natural Science Basic Research Plan in Shaanxi Province of China Grants 2016JZ013
文摘Tracking moving wideband sound sources is one of the most challenging issues in the acoustic array signal processing which is based on the direction of arrival(DOA) estimation. Compressive sensing(CS) is a recent theory exploring the signal sparsity representation, which has been proved to be superior for the DOA estimation. However, the spatial aliasing and the offset at endfire are the main obstacles for CS applied in the wideband DOA estimation. We propose a particle filter based compressive sensing method for tracking moving wideband sound sources. First, the initial DOA estimates are obtained by wideband CS algorithms. Then, the real sources are approximated by a set of particles with different weights assigned. The kernel density estimator is used as the likelihood function of particle filter. We present the results for both uniform and random linear array. Simulation results show that the spatial aliasing is disappeared and the offset at endfire is reduced. We show that the proposed method can achieve satisfactory tracking performance regardless of using uniform or random linear array.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51675026)
文摘Motion accuracy of space manipulators has direct effects on the ability of the systems to perform specified tasks. However, some design variables are inherently interval parameters due to uncertainties in geometric structures, material properties, and so on. This paper presents Chebyshev inclusion function(CIF) for approximating the dynamic responses function of parametrically excited systems. Motion accuracy reliability(MAR) of space manipulators was evaluated based on mechanism reliability analysis methods and interval uncertainty model. To illustrate the accuracy of the proposed method, a two-link manipulator with interval parameters was demonstrated. The results showed that the proposed method required much fewer samples to obtain more accurate reliability compared with the traditional Monte Carlo simulation(MCS). Finally, the sensitivity analysis was performed to facilitate the optimization design by using global sensitivity analysis.
基金Supported by National Natural Science Foundation of China(Grant Nos.51675425,52075441)Shaanxi Provincial Key Research Program Project of China(Grant No.2020ZDLGY06-09)+1 种基金Dongguan Municipal Social Science and Technology Development(key)Project of China(Grant No.20185071021600)Science and Technology on Micro-system Laboratory Foundation of China(Grant No.6142804200405).
文摘Wideband acoustic imaging,which combines compressed sensing(CS)and microphone arrays,is widely used for locating acoustic sources.However,the location results of this method are unstable,and the computational efficiency is low.In this work,in order to improve the robustness and reduce the computational cost,a DCS-SOMP-SVD compressed sensing method,which combines the distributed compressed sensing using simultaneously orthogonal matching pursuit(DCS-SOMP)and singular value decomposition(SVD)is proposed.The performance of the DCS-SOMP-SVD is studied through both simulation and experiment.In the simulation,the locating results of the DCS-SOMP-SVD method are compared with the wideband BP method and the DCS-SOMP method.In terms of computational efficiency,the proposed method is as efficient as the DCS-SOMP method and more efficient than the wideband BP method.In terms of locating accuracy,the proposed method can still locate all sources when the signal to noise ratio(SNR)is−20 dB,while the wideband BP method and the DCS-SOMP method can only locate all sources when the SNR is higher than 0 dB.The performance of the proposed method can be improved by expanding the frequency range.Moreover,there is no extra source in the maps of the proposed method,even though the target sparsity is overestimated.Finally,a gas leak experiment is conducted to verify the feasibility of the DCS-SOMP-SVD method in the practical engineering environment.The experimental results show that the proposed method can locate both two leak sources in different frequency ranges.This research proposes a DCS-SOMP-SVD method which has sufficient robustness and low computational cost for wideband acoustic imaging.
文摘BACKGROUND Venous thromboembolism(VTE)is a major cause of unexpected and perioperative in-hospital deaths.It is characterized by high morbidity,high mortality,high misdiagnosis rate,and high missed diagnosis rates.VTE is a common postoperative complication in cancer patients.VTE is preventable,and early identification of risk factors leading to VTE and appropriate early preventive actions can reduce its occurrence and mortality.Presently,there is no uniform standard for the prevention and control of VTE in clinical practice,and hospitals in China lack mature and effective protocols for the assessment,prevention,and treatment of VTE.AIM To explore whether an early warning program could influence the occurrence of deep vein thrombosis(DVT)postoperatively.METHODS This is a comparative retrospective cohort study,which enrolled patients who underwent laparotomic or laparoscopic gastrointestinal tumor resection for gastrointestinal cancer between January 2016 and December 2019.Patients were divided into a control group and an early warning group depending on whether or not the early warning program was implemented.A venous thromboembolism prevention and control team was established.The outcomes included the occurrence of DVT,the correct rate of VTE assessment,the coagulation indicators,and the mastery of VTE knowledge by the nurses.RESULTS A total of 264 patients were included in this study,with 128 patients in the control group and 136 patients in the early warning group.The occurrence rate of DVT in the early warning group was 6.6%(9/136),compared with 14.1%(18/128)in the control group(P<0.05).The correct rates of VTE risk assessment by the nurses and standard implementation rate of VTE preventive measures were 86.8%vs 65.6%and 80.2%vs 57.8%in early warning and control groups,respectively(all P<0.001).The independent factors associated with postoperative DVT occurrence were age(OR=1.083,95%CI:1.070-3.265,P=0.032),Hyperlipidemia(OR=1.127,95%CI:1.139-2.564,P=0.042),preoperative high VTE risk(OR=2.131,95%CI:1.085-5.178,P=0.001),time of operation(OR=2.268,95%CI:2.005-5.546,P=0.026)and not adoption of early warning prevention(OR=3.747,95%CI:1.523-6.956,P=0.017).CONCLUSION The early warning strategy was independently associated with the decreasing occurrence of VTE,and it might be suitable for protection from VTE in patients undergoing gastrointestinal cancer surgery.
基金Regional Fund Project of National Natural Science Foundation of China(No.81460712)the Young and Middle-aged Teachers’Scientific Research Basic Ability Enhancement Project of Guangxi Universities(No.2020KY07026)Guangxi Graduate Education Innovation Program(No.YCXJ2021035)。
文摘In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.
基金Regional Fund Project of National Natural Science Foundation of China(No.81460712)the Young and Middle-aged Teachers’Scientific Research Basic Ability Enhancement Project of Guangxi Universities(No.2020KY07026)Guangxi Graduate Education Innovation Program(No.YCXJ2021035)。
文摘Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were randomly divided into five groups,A:H9C2 cell group B:H9C2 cells+H2O2 model group C:H9C2 cells+H2O2 model+Qishen Yiqi group D:H9C2 cells+H2O2 model+Qishen Yiqi+wort group E:H9C2 cells+H2O2 model+Qishenyiqi+5-HD group,the drug intervention is according to the corresponding conditions.CCK-8 method was used to detect the cell activity of each group;Western blot was used to detect the expression of AKT and P-Akt proteins in myocardial cells in each group.The current was recorded by the standard patch clamp whole cell recording method,and the current was collected and analyzed by Pclamp6.0 software.Results:CCK-8 test results showed that compared with group A,the activity of myocardial cells in group B was significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,the difference in group C was statistically significant(P<0.01);compared with C,cardiomyocyte activity in D and E group were significantly decreased,and the difference was statistically significant(P<0.05);WB results showed that compared with A,p-Akt protein expression in B,C,D and E groups were significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,p-Akt protein expression in C,D and E group were significantly increased,and the difference was statistically significant(P<0.01),but there was no significant difference in AKT expression among groups(P>0.05);The results of whole cell patch clamp experiment showed that the outward current of B was significantly increased compared with that of A,and the difference between groups was statistically significant(P<0.01);compared with group B,cardiomyocytes in group C further increased the outward current,and the difference between groups was statistically significant(P<0.01);compared with C,the current of D and E group were significantly decreased,with statistical significance between groups(P<0.01).Conclusion:QishenYiqi dropping pills can protect cardiomyocytes by activating p-Akt protein expression and KATP channel opening in H9C2 cardiomyocytes.
基金supported by the National Funds for Distinguished Young Scientists(61825503)the National Natural Science Foundation of China(62288102,62205277 and 62322508).
文摘MicroRNAs (miRNAs) are pivotal modulators of plant development and host-virus interactions. However, the roles and action modes of specific miRNAs involved in viral infection and host susceptibility remain largely unclear. In this study, we show that Rice ragged stunt virus (RRSV) infection caused increased accumulation of miR319 but decreased expression of miR319-regulated TCP (TEOSINTE BRANCHED/ CYCLOIDEA/PCF) genes, especially TCP21, in rice plants. Transgenic rice plants overexpressing miP,319 or downregulating TCP21 exhibited disease-like phenotypes and showed significantly higher susceptibility to RRSV in comparison with the wild-type plants. In contrast, only mild disease symptoms were observed in RRSV-infected lines overexpressing TCP21 and especially in the transgenic plants overexpressing miR319- resistant TCP21. Both RRSV infection and overexpression of miR319 caused the decreased endogenous jasmonic acid (JA) levels along with downregulated expression of JA biosynthesis and signaling-related genes in rice. However, treatment of rice plants with methyl jasmonate alleviated disease symptoms caused by RRSV and reduced virus accumulation. Taken together, our results suggest that the induction of miR319 by RRSV infection in rice suppresses JA-mediated defense to facilitate virus infection and symp- tom development.
基金supported by grants from the National Natural Science Foundation of China(31900231)National Science Foundation of Guangdong Province(2018A030310505)to C.Y.+3 种基金the National Natural Science Foundation of China(31870171 and 31671467),toC.G.the Youth Innovation Promotion Association,Chinese Academy of Sciences(2017399)the National Key R&D Program of China(2019YFC1711102)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA13020500)to M.L.,and the National Natural Science Foundation of China(31701246)to W.S.
文摘Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants.In this study,we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes(ATGs).We showed that depletion of the ELONGATED HYPOCOTYL 5(HY5),a key component of light signaling,leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments,contributing to higher expression oiATGs.HY5 interacts with and recruits HISTONE DEACETYLASE 9(HDA9)to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3.Furthermore,we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci,leading to their depression and thereby activated autophagy.Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway.Collectively,our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants.
基金supported by the National Natural Science Foundation of China (81401932)the Beijing Natural Science Foundation (7154246)
文摘In this study, we aimed to investigate the functional network changes that occur in patients with lower back pain(LBP). We also investigated the link between LBP and the small-world properties of functional networks within the brain. Functional MRI(fMRI) was performed on 20 individuals with LBP and 17 age and gender-matched normal controls during the resting state. The severity of the pain in the individuals with LBP ranged from 5 to 8 on a 0–10 scale, with 0 indicating no pain. Network-based statistics were performed to investigate the differences between the brain networks of individuals with LBP and those of normal controls. Several small-world parameters of brain networks were calculated, including the clustering coefficient, characteristic path length, local efficiency, and global efficiency. These criteria reflect the overall network efficiency. The brain networks in the individuals with LBP due to herniation of a lumbar disc demonstrated a significantly longer characteristic path length as well as a lower clustering coefficient, global efficiency, and local efficiency compared to those in control subjects. We found that LBP patients tended to have unstable and inefficient brain networks when compared with healthy controls. In addition, LBP individuals showed significantly decreased functional connectivity in the anterior cingulate cortex, middle cingulate cortex, post cingulate cortex, inferior frontal gyrus, middle temporal gyrus, occipital gyrus, postcentral gyrus, precentral gyrus, supplementary motor area, thalamus, fusiform, caudate, and cerebellum. We believe that these regions may be involved in the pathophysiology of lower back pain.
基金National Research Foundation of Korea(NRF)(2012R1A2A2A01005544)China Scholarship Council(CSC)
文摘We propose and demonstrate an agile X-band signal synthesizer with ultralow phase noise based on all-fiberphotonic techniques for radar applications. It shows phase noise of-145 dBc∕Hz(-152 dBc∕Hz) at 10 kHz(100 kHz) offset frequency for 10 GHz carrier frequency with integrated RMS timing jitter between 7.6 and 9.1 fs(integration bandwidth: 10 Hz–10 MHz) for frequencies from 9 to 11 GHz. Its frequency switching time is evaluated to be 135 ns with a 135 pHz frequency tuning resolution. In addition, the X-band linearfrequency-modulated signal generated by the proposed synthesizer shows a good pulse compression ratio approximating the theoretical value. In addition to the ultrastable X-band signals, the proposed synthesizer can also provide 0–1 GHz ultralow-jitter clocks for analog-to-digital converters(ADC) and digital-to-analog converters(DAC) in radar systems and ultralow-jitter optical pulse trains for photonic ADC in photonic radar systems.The proposed X-band synthesizer shows great performance in phase stability, switching speed, and modulation capability with robustness and potential low cost, which is enabled by an all-fiber-photonics platform and can be a compelling technology suitable for future X-band radars.
基金the financial support from the National Funds for Distinguished Young Scientists(61825503)the National Natural Science Foundation of China(62075101,61775101,and 61775103)the Natural Science Foundation of Jiangsu Province of China(BK20200095).
文摘Materials that exhibit visible luminescence upon X-ray irradiation show great potential in the medical and industrial fields.Pure organic materials have recently emerged as promising scintillators for X-ray detection and radiography,due to their diversified design,low cost,and facile preparation.However,recent progress in efficient radioluminescence has mainly focused on small molecules,which are inevitably associated with processability and repeatability issues.Here,a concise strategy is proposed to prepare radioluminescent polymers that exhibit multiple emission colors from blue to yellow with high brightness in an amorphous state by the radical copolymerization of negatively charged polyacrylic acid and different positively charged quaternary phosphonium salts.One of the obtained polymers exhibits excellent photostability under a high X-ray irradiation dosage of 27.35 Gy and has a detection limit of 149 nGy s^(−1).This performance is superior to that of conventional anthracene-based scintillators.Furthermore,by simply drop-casting a polymer methanol solution on a quartz plate,a transparent scintillator screen was successfully fabricated for X-ray imaging with a resolution of 8.7 line pairs mm^(−1).The pure organic phosphorescent polymers with a highly efficient radioluminescence were demonstrated for the first time,and the strategy reported herein offers a promising pathway to expand the application range of amorphous organic scintillators.
文摘(Molecular Plant 13,515-531;March 2020)After publication of our original manuscript,we became aware of errors in Figure 4.During the preparation of Figure 4C in this article as originally published,we inadvertently duplicated the image of hda9-1(MS-N)as that of the hda9-1-C(MS+N).Also,in Figure 4H,the image of pUBQ10:GFP-ATG8a/hda9-1(MS+L)was mistakenly a duplicate of pUBQ10:GFP-ATG8a/WT(MS+L)shown in Figure S7C.A corrected version of Figure 4 is shown below.The scientific conclusions of this article have not been affected by this correction.The authors apologize for not detecting this error prior to publication and for any inconvenience that may have been caused.
基金This work has been funded by China-Russia International Science and Technology Cooperation Base of Sea Buckthorn Processing Technology in Gansu Province(Science and Technology Department of Gansu Province),China-Russian Sea Buckthorn Joint Research Center(Ministry of Education of the People’s Republic of China)and Natural Science Foundation of Gansu Province(20JR10RA514).
文摘Hippophae rhamnoides ssp.sinensis(Rha)and H.tibetana(Tib)are two species of sea buckthorn which distribute in Qinghai-Tibet Plateau.Our results showed that both sea buckthorn leaf exhibited high total phenolic and flavonoid content,and Rha leaf showed much higher level.In addition,66 phenolic compounds were detected,belonging to classes of benzoic acid derivatives,flavonols,flavanols,flavanones,and so on.Classes of benzoic acid derivatives and flavonols were most abundant,among them ellagic acid and rutin were the most and second abundant phenols.Besides,methyl gallate,2,6-dihydroxybenzoic acid,4-hydroxybenzoic acid,gentisic acid,narcissin,astragalin,nicotiflorin,prunin,procyanidin B3,cyanidin 3-O-rutinoside chloride,delphinidin 3-glucoside,gallocatechin,taxifolin,aromadendrin and trans-3,3′,4′,5,5′,7-Hexahydroxyflavanone were the first time to be identified in SBT leaf.Furthermore,significant free radical scavenging effects of ABTS and DPPH,ferric reducing antioxidant ability,cellular antioxidant properties and anti-proliferation activity against HepG2 cells were observed in the aqueous ethanol extracts of sea buckthorn leaf.While Rha showed stronger inhibitory effects,which might due to the higher content of total phenolic and flavonoid profiles.Our finding clearly supported that Rha leaf as a wild and non-pollution plant could be a better choice as a healthy food resource.