AIM:To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma.METHODS:Ninety-three non-consanguineous colorectal carcinoma patients admitted to ...AIM:To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma.METHODS:Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study.Ninety-three peripheral venous blood samples,of approximately one milliliter from each patient,were collected betweenDecember 2009 and August 2010.The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit(Fermentas,CA) according to the manufacturer' s protocol.The single nucleotide polymorphisms(SNPs) of the liver-intestine cadherin(CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method(PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma.Typical samples that showed different migration bands in SSCP were confirmed by sequencing.Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method.RESULTS:There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis(TNM) grade,as well as with lymph node status,in 93 peripheral venous blood samples from colorectal carcinoma patients.The genotype frequencies of A/C,A/A,and C/C were 12.90%,33.33% and 53.76%,respectively.There was a significant correlation between lymph node metastasis,TNM grade,and the genotype distribution(P < 0.05).The C/C genotype raised the risk of lymph node metastasis and the TNM grade.There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes(P = 0.003 and P = 0.013,respectively).Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade.The difference between the TNM grades,as well as the lymph node metastasis of the two alleles,was statistically significant(P < 0.01).CONCLUSION:The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.展开更多
The transition dipole moments(TDMs) of ultracold85 Rb133 Cs molecules between the lowest vibrational ground level, (X^1Σ~+( v= 0, J= 1), and the two excited rovibrational levels, 2~3Π0+(v′= 10, J′= 2) and...The transition dipole moments(TDMs) of ultracold85 Rb133 Cs molecules between the lowest vibrational ground level, (X^1Σ~+( v= 0, J= 1), and the two excited rovibrational levels, 2~3Π0+(v′= 10, J′= 2) and 2~1Π1(v′= 22,J′= 2), are measured using depletion spectroscopy. The ground-state85 Rb133 Cs molecules are formed from cold mixed component atoms via the 2~3Π0-( v= 11, J= 0) short-range level, then detected by time-of-flight mass spectrum. A home-made external-cavity diode laser is used as the depletion laser to couple the ground level and the two excited levels. Based on the depletion spectroscopy, the corresponding TDMs are then derived to be 3.5(2)×10^(-3)eαα and 1.6(1)×10^(-2)eαα, respectively, where 0)(60 represents the atomic unit of electric dipole moment. The enhance of TDM with nearly a factor of 5 for the 21Π1(v′= 22, J′= 2) excited level means that it has stronger coupling with the ground level. It is meaningful to find more levels with much more strong coupling strength by the represented depletion spectroscopy to realize direct stimulated Raman adiabatic passage transfer from scattering atomic states to deeply molecular states.展开更多
This paper discusses the design and implementation of a low cost multi-sensor integrated attitude determination system for small unmanned aerial vehicles( UAVs),which uses strapdown inertial navigation system( SINS) b...This paper discusses the design and implementation of a low cost multi-sensor integrated attitude determination system for small unmanned aerial vehicles( UAVs),which uses strapdown inertial navigation system( SINS) based on micro electromechanical system( MEMS) inertial sensors,commercial GPS receiver,and 3-axis magnetometer.MEMS-SINS initial attitude determination cannot be well performed for the reason that the MEMS inertial sensors biases are time-varying and poor repeatability,therefore in this paper the magnetometer and inclinometer are used to assist the MEMS-SINS initial attitude determination and MEMS inertial sensors field calibration.Furthermore,the attitude determination algorithms are presented to estimate the full attitude during GPS signal outage and non-accelerating situation.Additionally,the attitude information estimation results are compared with the reference of the non-magnetic marble platform and 3-axis turntable.Then the attitude estimation precision satisfies the requirement of attitude measurement for small UAVs during GPS signal outage and availability.Finally,the small UAV autonomous flight test results show that the low cost and real-time attitude determination system can yield continuous,reliable and effective attitude information for small UAVs.展开更多
基金Supported by 2010A310011 Henan Provincial Department of Education on Natural Science Research Projects
文摘AIM:To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma.METHODS:Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study.Ninety-three peripheral venous blood samples,of approximately one milliliter from each patient,were collected betweenDecember 2009 and August 2010.The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit(Fermentas,CA) according to the manufacturer' s protocol.The single nucleotide polymorphisms(SNPs) of the liver-intestine cadherin(CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method(PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma.Typical samples that showed different migration bands in SSCP were confirmed by sequencing.Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method.RESULTS:There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis(TNM) grade,as well as with lymph node status,in 93 peripheral venous blood samples from colorectal carcinoma patients.The genotype frequencies of A/C,A/A,and C/C were 12.90%,33.33% and 53.76%,respectively.There was a significant correlation between lymph node metastasis,TNM grade,and the genotype distribution(P < 0.05).The C/C genotype raised the risk of lymph node metastasis and the TNM grade.There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes(P = 0.003 and P = 0.013,respectively).Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade.The difference between the TNM grades,as well as the lymph node metastasis of the two alleles,was statistically significant(P < 0.01).CONCLUSION:The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.
基金Supported by the National Key Research and Development Program of China under Grant No 2017YFA0304203the National Natural Science Foundation of China under Grant Nos 61675120 and 11434007+3 种基金the National Natural Science Foundation of China for Excellent Research Team under Grant No 61121064the Shanxi Scholarship Council of China,the 1331KSCthe PCSIRT under Grant No IRT13076the Applied Basic Research Project of Shanxi Province under Grant No 201601D202008
文摘The transition dipole moments(TDMs) of ultracold85 Rb133 Cs molecules between the lowest vibrational ground level, (X^1Σ~+( v= 0, J= 1), and the two excited rovibrational levels, 2~3Π0+(v′= 10, J′= 2) and 2~1Π1(v′= 22,J′= 2), are measured using depletion spectroscopy. The ground-state85 Rb133 Cs molecules are formed from cold mixed component atoms via the 2~3Π0-( v= 11, J= 0) short-range level, then detected by time-of-flight mass spectrum. A home-made external-cavity diode laser is used as the depletion laser to couple the ground level and the two excited levels. Based on the depletion spectroscopy, the corresponding TDMs are then derived to be 3.5(2)×10^(-3)eαα and 1.6(1)×10^(-2)eαα, respectively, where 0)(60 represents the atomic unit of electric dipole moment. The enhance of TDM with nearly a factor of 5 for the 21Π1(v′= 22, J′= 2) excited level means that it has stronger coupling with the ground level. It is meaningful to find more levels with much more strong coupling strength by the represented depletion spectroscopy to realize direct stimulated Raman adiabatic passage transfer from scattering atomic states to deeply molecular states.
基金Sponsored by the China Postdoctoral Science Foundation(Grant No.2013M540857)the Fundamental Research Funds for the Central Universities(Grant No.FRF-TP-14-019A1)
文摘This paper discusses the design and implementation of a low cost multi-sensor integrated attitude determination system for small unmanned aerial vehicles( UAVs),which uses strapdown inertial navigation system( SINS) based on micro electromechanical system( MEMS) inertial sensors,commercial GPS receiver,and 3-axis magnetometer.MEMS-SINS initial attitude determination cannot be well performed for the reason that the MEMS inertial sensors biases are time-varying and poor repeatability,therefore in this paper the magnetometer and inclinometer are used to assist the MEMS-SINS initial attitude determination and MEMS inertial sensors field calibration.Furthermore,the attitude determination algorithms are presented to estimate the full attitude during GPS signal outage and non-accelerating situation.Additionally,the attitude information estimation results are compared with the reference of the non-magnetic marble platform and 3-axis turntable.Then the attitude estimation precision satisfies the requirement of attitude measurement for small UAVs during GPS signal outage and availability.Finally,the small UAV autonomous flight test results show that the low cost and real-time attitude determination system can yield continuous,reliable and effective attitude information for small UAVs.