不吸收缝线穿骨修复治疗急性髌骨脱位合并髌骨骨软骨骨折的疗效观察

目的探讨不吸收缝线穿骨修复治疗急性髌骨脱位合并髌骨骨软骨骨折(OCFs)的临床疗效。方法回顾性分析2016年9月-2019年9月该院诊治的17例急性髌骨脱位合并髌骨OCFs患者的临床资料,17例患者均为急性损伤。其中,男8例,女9例;年龄13~39岁,平均20.3岁;左侧10例,右侧7例。记录患者术前术后关节活动范围、单腿跳远测试、大腿周径、视觉模拟评分(VAS)、国际膝关节文献委员会(IKDC)评分和Lysholm膝关节评分。结果17例患者均获随访,随访时间12~18个月,平均15个月。术后第12个月复查CT显示:所有患者均获得骨性愈合,未出现骨块脱落。末次随访时,IKDC评分、Lysholm膝关节评分和VAS与术前比较,差异均有统计学意义(P<0.05)。末次随访时,患侧大腿周径、单腿跳远测试和膝关节活动范围与术前健侧比较,差异无统计学意义(P>0.05)。结论不吸收缝线穿骨修复治疗急性髌骨脱位合并髌骨OCFs,临床疗效满意,可以获得满意的固定强度,OCFs可得到骨性愈合,且无金属内固定物,无需行二次手术取出,同时还能恢复良好的膝关节功能。

Clinical effect of Rehabmtation Treating combined with Intra-Articular Injection of Sodium Hyaluronate for Exercise induced knee osteoarthritis

Objective: To study the clinical effect of rehabmtation treating combined with intra-articular injection of sodium hyaluronate for exercise induced knee osteoarthritis, and to research the function, biomechanics of knee joint, and inflammatory factor in articular cavity. Method:Selecting 120 patients with exercise induced knee osteoarthritis, then they were randomly divided into control group (n=60) and intervene group (n=60). The patients in the control group were given conventional rehabmtation treating, and the intervene group were given intra-articular injection of sodium hyaluronate on this basic, they were treated 4 weeks. Evaluating the function of knee joint;conduct isokinetic muscle strength test (determination of the angIllar velocity were 60°/S, 180°/S);test the ratio of muscle peak torque and four muscles centripetal centripetal peak torque and peak torque, work load of femoral knee joints after (simultaneous determination of the angular velocity were 60°/S);detect the inflammatory factor in the joint fluid. Results: After the treatment, clinical effect in intervene group was superior to that in control group,but had no statistical significance;after the treatment, the score of function of knee joint in the two groups were higher than before treatment, and had statistical significance, and the score of function of knee joint in the intervene group were higher than control group, and the difference had statistical significance;Angular velocity of 60°/S and 180°/S the two groups peak torque, work load and the H/Q values were significantly increased, the intervene group were higher than control group, and the difference had statistical significance;inflammatory factor in the two groups were significantly decreased, the intervene group were lower than control group, and the difference had statistical significance.Conclusions: On the basic of rehabmtation treating, the treatment of intra-articular injection of sodium hyaluronate can improve the function of knee joint through improving the knee muscle strength, the level of their participation in the activities of the biomechanics of knee joint, and the inflammatory factor in the joint fluid.

Research on Practice Problems and Strategies for Professional Master's Degree Candidates for Agriculture

Taking the practice status of professional master’s degree candidates for agriculture in Anhui Agricultural University as an example,this paper analyzes the key factors affecting the practical training of graduate students in this major,and puts forward some strategies for improving the practice quality of professional master’s degree candidates.In terms of training professional master’s degree candidates,it is considered that agricultural colleges and universities should start with collaborative education,supervision and assessment mechanism,practice content and form,make full use of a large number of agricultural enterprise resources owned by colleges and universities and a good cooperation foundation in enterprises,so as to achieve the purpose of improving the practice quality of professional master’s degree candidates.

Perspectives in application of biosensors for the modernization of traditional Chinese medicine

Biosensor is an instrument which is sensitive to biological material and converts its concentration into electrical signals.Organisms such as enzymes, antibodies, tissues, cells and so on can selectively identify specific substances. Thesemolecular recognition functions can be combined with the target, such as the binding of antibodies and antigens, and thebinding of enzymes to the substrate through the recognition process. Biosensor has the advantages of high specificity,high sensitivity, fast reaction speed, low cost and easy operation. It has wide application prospect in food, pharmacy,chemical industry, clinical examination, biomedicine, environmental monitoring and so on, especially as a newtechnology means, in the field of modern herbal medicine research influence. Studies have demonstrated that thebiosensing technology has been applied to, traditional Chinese medicine （TCM） targets, isolation and purification ofTCM, the mechanism of TCM, quality control of TCM, the active ingredients detection of TCM and other basicresearches. Biosensor technology has made an important contribution to the research of modern herbal medicine, and hasbecome a Hot-spot in future research.

An orally-available monovalent SMAC mimetic compound as a broad-spectrum antiviral

DearEditor,The coronavirus disease 2019(COVID-19)pandemic caused great global morbidity and mortality concurrent with astronomical socioeconomic damages and losses.Until now,the culpable pathogen,SARS-CoV-2,has infected over 6 hundred million people resulting in more than 6 million deaths worldwide(WHO SARSCoV-2 cases).There is an urgent need for rapid development of effective and affordable therapeutics for COVID-19 patients.

ASFV transcription reporter screening system identifies ailanthone as a broad antiviral compound

African swine fever(ASF)is an acute,highly contagious and deadly viral disease in swine that jeopardizes the worldwide pig industry.Unfortunately,there are no authoritative vaccine and antiviral drug available for ASF control.African swine fever virus(ASFV)is the etiological agent of ASF.Among the ASFV proteins,p72 is the most abundant component in the virions and thus a potential target for anti-ASFV drug design.Here,we con-structed a luciferase reporter system driven by the promoter of p72,which is transcribed by the co-transfected ASFV RNA polymerase complex.Using this system,we screened over 3200 natural product compounds and obtained three potent candidates against ASFV.We further evaluated the anti-ASFV effects and proved that among the three candidates,ailanthone(AIL)inhibits the replication of ASFV at the nanomolar concentration(IC_(50)=15 nmol/L).Our in vitro experiments indicated that the antiviral effect of AIL is associated with its inhibition of the HSP90-p23 cochaperone.Finally,we showed the antiviral activity of AIL on Zika virus and hepatitis B virus(HBV),which supports that AIL is a potential broad-spectrum antiviral agent.