目的筛选并鉴定与膀胱癌侵袭性相关的环状RNA(circRNA)。方法通过转录组测序,分析来自9例组织(癌旁、非肌层浸润膀胱癌和肌层浸润膀胱癌组织各3例)的circRNA测序结果。qPCR检测20例临床膀胱癌样本(非肌层浸润和肌层浸润各10例)中候选cir...目的筛选并鉴定与膀胱癌侵袭性相关的环状RNA(circRNA)。方法通过转录组测序,分析来自9例组织(癌旁、非肌层浸润膀胱癌和肌层浸润膀胱癌组织各3例)的circRNA测序结果。qPCR检测20例临床膀胱癌样本(非肌层浸润和肌层浸润各10例)中候选circRNA表达水平。分析20例临床膀胱癌样本的病理特点与候选circRNA表达水平变化的相关性。qPCR检测4种膀胱癌细胞系中候选circRNA本底表达水平。Transwell侵袭实验检测膀胱癌细胞侵袭能力。结果通过对非肌层浸润膀胱癌与肌层浸润膀胱癌组织测序结果的比对,发现305个具有显著差异的circRNA(210个显著上调,95个显著下调),筛选出其中3个显著上调的候选circRNA( circST6GALNAC6、circPRUNE2、circMYH11)。qPCR检测发现,circST6GALNAC6与circMYH11 在肌层浸润膀胱癌组织中的表达水平显著高于非肌层浸润膀胱癌组织( P <0.01),与circRNA测序数据相符;而circPRUNE2在上述两种膀胱癌组织中的表达差异无统计学意义( P >0.05)。circST6GALNAC6和circMYH11与膀胱肿瘤大小、病理分期和分级存在统计学意义上的相关性,肿瘤恶性程度越高,两者表达水平越高。此外,在膀胱癌细胞系实验中也证实,circST6GALNAC6和circMYH11的表达水平与癌细胞的侵袭性相关。结论本研究初步验证了circST6GALNAC6和circMYH11两种circRNA与膀胱癌侵袭能力的相关性。展开更多
Endothelial PAS domain protein 1（EPAS1）, also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this ...Endothelial PAS domain protein 1（EPAS1）, also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this study, polymorphisms of EPAS1 were detected in 217 individuals from 2 Tibetan pig populations and 3 low-altitude pig breeds by DNA pooling, PCR-SSCP, PCR-RFLP and DNA sequencing methods. A total of 14 synonymous polymorphisms were identified in the coding region. The analysis suggested that SNP1（G963A）, SNP7（C1632T）, SNP10（G1929A） and SNP11（G1947A） showed potential association with high-altitude environment because of their particular variation patterns in Tibetan pigs. Linkage disequilibrium（LD） of these SNPs was analyzed. One common LD block including 5 SNPs clustering in exon 12 was identified in all studied pig populations. Haplotype H1（AGGTC） in LD block was dominant in Tibetan pigs（76.6 and 74.2% in Linzhi（LZ） and Chayu（CY） pigs, respectively） and segregated at higher frequency than that in low-altitude pig breeds（52.3, 58.7 and 56.2% in Wuzhishan（WZS）, Min（M） and Laiwu（LW） pigs, respectively）, indicating that H1 may relate to adaptation to high altitude in Tibetan pigs. These findings raise hope that EPAS1 gene can be a candidate gene that involved in adaptation of high altitude in Tibetan pigs.展开更多
目的系统评价米卡芬净预防血液系统恶性肿瘤患者侵袭性真菌感染(IFIs)的有效性及安全性,为临床治疗提供循证参考。方法计算机检索PubMed、Embase、Cochrane图书馆、中国知网(CNKI)、万方数据,检索时限为建库起至2021年1月,收集米卡芬净...目的系统评价米卡芬净预防血液系统恶性肿瘤患者侵袭性真菌感染(IFIs)的有效性及安全性,为临床治疗提供循证参考。方法计算机检索PubMed、Embase、Cochrane图书馆、中国知网(CNKI)、万方数据,检索时限为建库起至2021年1月,收集米卡芬净(试验组)对比常规抗真菌药物(两性霉素B及三唑类抗真菌药,对照组)的随机对照试验(RCT),对符合纳入标准的临床研究进行资料提取并采用Cochrane系统评价员手册5.0.2进行质量评价后,采用Rev Man 5.3统计软件对突破性IFIs、真菌感染死亡率、全因死亡率及因不良反应停药的发生率进行Meta分析。结果共纳入9项RCT,合计2 479例患者。Meta分析结果显示,试验组患者突破性IFIs发生率[OR=0.74,95%CI(0.50,1.07),P=0.11]、真菌感染死亡率[OR=0.73,95%CI(0.46,1.17),P=0.19]和全因死亡率[OR=0.94,95%CI(0.69,1.28),P=0.7]与对照组相比,差异无统计学意义;因不良反应停药的发生率[OR=0.46,95%CI(0.32,0.66),P<0.0001]显著低于对照组,差异有统计学意义。结论米卡芬净用于预防血液系统恶性肿瘤患者IFIs的效果与两性霉素B及三唑类抗真菌药物相当,且安全性更高。展开更多
Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-sta...Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.展开更多
文摘目的筛选并鉴定与膀胱癌侵袭性相关的环状RNA(circRNA)。方法通过转录组测序,分析来自9例组织(癌旁、非肌层浸润膀胱癌和肌层浸润膀胱癌组织各3例)的circRNA测序结果。qPCR检测20例临床膀胱癌样本(非肌层浸润和肌层浸润各10例)中候选circRNA表达水平。分析20例临床膀胱癌样本的病理特点与候选circRNA表达水平变化的相关性。qPCR检测4种膀胱癌细胞系中候选circRNA本底表达水平。Transwell侵袭实验检测膀胱癌细胞侵袭能力。结果通过对非肌层浸润膀胱癌与肌层浸润膀胱癌组织测序结果的比对,发现305个具有显著差异的circRNA(210个显著上调,95个显著下调),筛选出其中3个显著上调的候选circRNA( circST6GALNAC6、circPRUNE2、circMYH11)。qPCR检测发现,circST6GALNAC6与circMYH11 在肌层浸润膀胱癌组织中的表达水平显著高于非肌层浸润膀胱癌组织( P <0.01),与circRNA测序数据相符;而circPRUNE2在上述两种膀胱癌组织中的表达差异无统计学意义( P >0.05)。circST6GALNAC6和circMYH11与膀胱肿瘤大小、病理分期和分级存在统计学意义上的相关性,肿瘤恶性程度越高,两者表达水平越高。此外,在膀胱癌细胞系实验中也证实,circST6GALNAC6和circMYH11的表达水平与癌细胞的侵袭性相关。结论本研究初步验证了circST6GALNAC6和circMYH11两种circRNA与膀胱癌侵袭能力的相关性。
基金supported by the National Natural Science Foundation of China(31272403)the Determination of Molecular Characterization for Genetically Modified Organism,China(2013ZX08012-002)the Livestock and Poultry Sharing Platform in China(2013)
文摘Endothelial PAS domain protein 1（EPAS1）, also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this study, polymorphisms of EPAS1 were detected in 217 individuals from 2 Tibetan pig populations and 3 low-altitude pig breeds by DNA pooling, PCR-SSCP, PCR-RFLP and DNA sequencing methods. A total of 14 synonymous polymorphisms were identified in the coding region. The analysis suggested that SNP1（G963A）, SNP7（C1632T）, SNP10（G1929A） and SNP11（G1947A） showed potential association with high-altitude environment because of their particular variation patterns in Tibetan pigs. Linkage disequilibrium（LD） of these SNPs was analyzed. One common LD block including 5 SNPs clustering in exon 12 was identified in all studied pig populations. Haplotype H1（AGGTC） in LD block was dominant in Tibetan pigs（76.6 and 74.2% in Linzhi（LZ） and Chayu（CY） pigs, respectively） and segregated at higher frequency than that in low-altitude pig breeds（52.3, 58.7 and 56.2% in Wuzhishan（WZS）, Min（M） and Laiwu（LW） pigs, respectively）, indicating that H1 may relate to adaptation to high altitude in Tibetan pigs. These findings raise hope that EPAS1 gene can be a candidate gene that involved in adaptation of high altitude in Tibetan pigs.
文摘目的系统评价米卡芬净预防血液系统恶性肿瘤患者侵袭性真菌感染(IFIs)的有效性及安全性,为临床治疗提供循证参考。方法计算机检索PubMed、Embase、Cochrane图书馆、中国知网(CNKI)、万方数据,检索时限为建库起至2021年1月,收集米卡芬净(试验组)对比常规抗真菌药物(两性霉素B及三唑类抗真菌药,对照组)的随机对照试验(RCT),对符合纳入标准的临床研究进行资料提取并采用Cochrane系统评价员手册5.0.2进行质量评价后,采用Rev Man 5.3统计软件对突破性IFIs、真菌感染死亡率、全因死亡率及因不良反应停药的发生率进行Meta分析。结果共纳入9项RCT,合计2 479例患者。Meta分析结果显示,试验组患者突破性IFIs发生率[OR=0.74,95%CI(0.50,1.07),P=0.11]、真菌感染死亡率[OR=0.73,95%CI(0.46,1.17),P=0.19]和全因死亡率[OR=0.94,95%CI(0.69,1.28),P=0.7]与对照组相比,差异无统计学意义;因不良反应停药的发生率[OR=0.46,95%CI(0.32,0.66),P<0.0001]显著低于对照组,差异有统计学意义。结论米卡芬净用于预防血液系统恶性肿瘤患者IFIs的效果与两性霉素B及三唑类抗真菌药物相当,且安全性更高。
基金This research is supported by Science and Technology Research Project of Shanghai Greening and City Appearance Administration in 2019(G192402).
文摘Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.
