Maintaining glutamate homeostasis after hypoxic ischemia is important for synaptic function and neural cell activity,and regulation of glutamate transport between astrocyte and neuron is one of the important modalitie...Maintaining glutamate homeostasis after hypoxic ischemia is important for synaptic function and neural cell activity,and regulation of glutamate transport between astrocyte and neuron is one of the important modalities for reducing glutamate accumulation.However,further research is needed to investigate the dynamic changes in and molecular mechanisms of glutamate transport and the effects of glutamate transport on synapses.The aim of this study was to investigate the regulatory mechanisms underlying Notch pathway mediation of glutamate transport and synaptic plasticity.In this study,Yorkshire neonatal pigs(male,age 3 days,weight 1.0–1.5 kg,n=48)were randomly divided into control(sham surgery group)and five hypoxic ischemia subgroups,according to different recovery time,which were then further subdivided into subgroups treated with dimethyl sulfoxide or a Notch pathway inhibitor(N-[N-(3,5-difluorophenacetyl-l-alanyl)]-S-phenylglycine t-butyl ester).Once the model was established,immunohistochemistry,immunofluorescence staining,and western blot analyses of Notch pathway-related proteins,synaptophysin,and glutamate transporter were performed.Moreover,synapse microstructure was observed by transmission electron microscopy.At the early stage(6–12 hours after hypoxic ischemia)of hypoxic ischemic injury,expression of glutamate transporter excitatory amino acid transporter-2 and synaptophysin was downregulated,the number of synaptic vesicles was reduced,and synaptic swelling was observed;at 12–24 hours after hypoxic ischemia,the Notch pathway was activated,excitatory amino acid transporter-2 and synaptophysin expression was increased,and the number of synaptic vesicles was slightly increased.Excitatory amino acid transporter-2 and synaptophysin expression decreased after treatment with the Notch pathway inhibitor.This suggests that glutamate transport in astrocytes-neurons after hypoxic ischemic injury is regulated by the Notch pathway and affects vesicle release and synaptic plasticity through the expression of synaptophysin.展开更多
Several consecutive extreme cold events impacted China during the first half of winter 2020/21,breaking the low-temperature records in many cities.How to make accurate climate predictions of extreme cold events is sti...Several consecutive extreme cold events impacted China during the first half of winter 2020/21,breaking the low-temperature records in many cities.How to make accurate climate predictions of extreme cold events is still an urgent issue.The synergistic effect of the warm Arctic and cold tropical Pacific has been demonstrated to intensify the intrusions of cold air from polar regions into middle-high latitudes,further influencing the cold conditions in China.However,climate models failed to predict these two ocean environments at expected lead times.Most seasonal climate forecasts only predicted the 2020/21 La Niña after the signal had already become apparent and significantly underestimated the observed Arctic sea ice loss in autumn 2020 with a 1-2 month advancement.In this work,the corresponding physical factors that may help improve the accuracy of seasonal climate predictions are further explored.For the 2020/21 La Niña prediction,through sensitivity experiments involving different atmospheric-oceanic initial conditions,the predominant southeasterly wind anomalies over the equatorial Pacific in spring of 2020 are diagnosed to play an irreplaceable role in triggering this cold event.A reasonable inclusion of atmospheric surface winds into the initialization will help the model predict La Niña development from the early spring of 2020.For predicting the Arctic sea ice loss in autumn 2020,an anomalously cyclonic circulation from the central Arctic Ocean predicted by the model,which swept abnormally hot air over Siberia into the Arctic Ocean,is recognized as an important contributor to successfully predicting the minimum Arctic sea ice extent.展开更多
Skyrmion bags are spin structures with arbitrary topological charges, each of which is composed of a big skyrmion and several small skyrmions. In this work, by using an in-plane alternating current(AC) magnetic field,...Skyrmion bags are spin structures with arbitrary topological charges, each of which is composed of a big skyrmion and several small skyrmions. In this work, by using an in-plane alternating current(AC) magnetic field, we investigate the spinwave modes of skyrmion bags, which behave differently from the clockwise(CW) rotation mode and the counterclockwise(CCW) rotation mode of skyrmions because of their complex spin topological structures. The in-plane excitation power spectral density shows that each skyrmion bag possesses four resonance frequencies. By further studying the spin dynamics of a skyrmion bag at each resonance frequency, the four spin-wave modes, i.e., a CCW-CW mode, two CW-breathing modes with different resonance strengths, and an inner CCW mode, appear as a composition mode of outer skyrmion–inner skyrmions. Our results are helpful in understanding the in-plane spin excitation of skyrmion bags, which may contribute to the characterization and detection of skyrmion bags, as well as the applications in logic devices.展开更多
Selective oxidation of alkanes to produce highvalue chemicals is an essential strategy and means to realize efficient utilization of resources.In this work,a strategy of lanthanum manganese mixed metal oxides(LMMO)reg...Selective oxidation of alkanes to produce highvalue chemicals is an essential strategy and means to realize efficient utilization of resources.In this work,a strategy of lanthanum manganese mixed metal oxides(LMMO)regulated via a facile ionic liquid(IL)-assisted hydrothermal method was proposed to construct the multifunctional catalysts,which exhibited excellent catalytic performance in the selective aerobic oxidation of cyclohexane.An 8.9%cyclohexane conversion with 90%KA oi(cyclohexanol and cyclohexanone)selectivity was achieved over the optimal LMMO catalyst under mild conditions.The effects of anion type,carbon chain length and concentration of ILs on the structure and properties of catalysts were investigated through various characterizations,indicating the structure-directing and template effect of ILs on the multifunctional catalysts.The formation of self-assembled spherical nanoparticles followed the"dissolution-nucleation-proliferation"mechanism with the introduction of 1-butyl-3-methylimidazolium hydrogen sulfate,ascribing the synergistic effect between the microenvironment of ILs and the hydrothermal environment.Importantly,the high reactive oxygen concentration redox capacity,and suitable basic sites of LMMO catalysts mediated by ILs enhance the activation of C-H bonds and molecular oxygen,simultaneously influencing the adsorption and desorption of the substrate.A comprehensive understanding of the high KA oil selectivity and radical reaction mechanism was elucidated based on in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS)and radical trapping experiments.The recycling and regeneration experiments further illuminated that the removal of adsorbed cyclohexanone acting on the LMMO catalyst was the key to achieve high KA oil selectivity.展开更多
BACKGROUND Penetrating neck injuries require prompt recognition,diagnosis and management of critical airways.This case demonstrates an emergent situation that a“medical negligence”was avoided with the aid of end-tid...BACKGROUND Penetrating neck injuries require prompt recognition,diagnosis and management of critical airways.This case demonstrates an emergent situation that a“medical negligence”was avoided with the aid of end-tidal carbon dioxide(ETCO2)waveform.CASE SUMMARY We report a case of malposition of the endotracheal tube into the right hemithoracic cavity for cervical knife trauma,resulting in pneumothorax.Tube placement was not confirmed during emergency airway management,and the patient was directly transferred to the emergency operation room.Assisted by ETCO2 and imaging examinations,the anesthetist timely noticed the absence of ETCO2 waveform and resolved this urgent situation before anesthesia induction.CONCLUSION This case emphasizes the necessity of ETCO2 waveform and/or X-ray confirmation of endotracheal intubation even in emergent situations.展开更多
Ferulic acid(FA)is a phenolic phytochemical with antioxidant and anti-inflammatory pharmacological effects.In recent years,the neuroprotective effects of FA have been studied extensively.Many researchers have attempte...Ferulic acid(FA)is a phenolic phytochemical with antioxidant and anti-inflammatory pharmacological effects.In recent years,the neuroprotective effects of FA have been studied extensively.Many researchers have attempted to use FA to prevent and treat neurological diseases and have made some progress.This paper mainly collated the study of the protective effect of FA on stroke and summarized the protective effect of FA on ischemic stroke and the potential protective effects of FA on neurovascular units.展开更多
Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recor...Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recording in various ex vivo methods. However, it is difficult to maintain the activity and morphology of neurons in slices from mice of 〉30 days old. Here we evaluated the N-methyI-D-glucamine （NMDG）- based artificial cerebrospinal fluid （aCSF） method for the preservation of interneurons in slices from mice of up to -6 months old and discussed the steps that may affect their quality during slicing. We found that the NMDG-aCSF method rescued more cells than sucrose-aCSF and successfully preserved different types of interneurons including parvalbumin- and somatostatin-positive interneurons. In addition, both the chemical and electrical synaptic signaling of interneurons were maintained. These results demonstrate that the NMDG-aCSF method is suitable for the preservation of interneurons, especially in studies of gap junctions.展开更多
Background:Observational studies suggest that hepatorenal syndrome(HRS)patients who receive pharmacological therapy before orthotopic liver transplantation display a post-transplant outcome similar to those without HR...Background:Observational studies suggest that hepatorenal syndrome(HRS)patients who receive pharmacological therapy before orthotopic liver transplantation display a post-transplant outcome similar to those without HRS.The aim of this study was to comprehensively compare and rank the pharmacological therapies for HRS.Methods:We reviewed PubMed,Elsevier,Medline,and the Cochrane Central Register of Controlled Trials(CENTRAL)for studies that were published between 1 January 1999 and 24 February 2018.The primary endpoint was reversal of HRS.The secondary endpoints were the changes in serum creatinine(Scr)and serum sodium.We evaluated the different therapeutic strategies using network meta-analysis on the basis of Bayesian methodology.Results:The study included 24 articles with 1,419 participants evaluating seven different therapeutic strategies for HRS.The most effective treatments to induce reversal of HRS were terlipressin plus albumin,noradrenaline plus albumin,and terlipressin,which had a surface under the cumulative ranking curve(SUCRA)of 0.086,0.151,and 0.451,respectively.The top two treatments for decreasing Scr were dopamine plus furosemide plus albumin(rank probability:0.620)and terlipressin plus albumin(rank probability:0.570).For increasing serum sodium,the optimal treatment was octreotide plus midodrine plus albumin(rank probability:0.800),followed by terlipressin plus albumin(rank probability:0.544).Conclusions:Terlipressin plus albumin and dopamine plus furosemide plus albumin should be prioritized for decreasing Scr in HRS,and octreotide plus midodrine plus albumin was the most effective at increasing serum sodium.Terlipressin plus albumin showed a comprehensive effect in both decreasing Scr and increasing serum sodium.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81871408 and 81271631(to XMW)National Science Foundation for Young Scientists of China,No.81801658(to YZ)+1 种基金Outstanding Scientific Fund of Shengjing Hospital,No.201402(to XMW)345 Talent Support Project of Shengjing Hospital,No.30B(to YZ)。
文摘Maintaining glutamate homeostasis after hypoxic ischemia is important for synaptic function and neural cell activity,and regulation of glutamate transport between astrocyte and neuron is one of the important modalities for reducing glutamate accumulation.However,further research is needed to investigate the dynamic changes in and molecular mechanisms of glutamate transport and the effects of glutamate transport on synapses.The aim of this study was to investigate the regulatory mechanisms underlying Notch pathway mediation of glutamate transport and synaptic plasticity.In this study,Yorkshire neonatal pigs(male,age 3 days,weight 1.0–1.5 kg,n=48)were randomly divided into control(sham surgery group)and five hypoxic ischemia subgroups,according to different recovery time,which were then further subdivided into subgroups treated with dimethyl sulfoxide or a Notch pathway inhibitor(N-[N-(3,5-difluorophenacetyl-l-alanyl)]-S-phenylglycine t-butyl ester).Once the model was established,immunohistochemistry,immunofluorescence staining,and western blot analyses of Notch pathway-related proteins,synaptophysin,and glutamate transporter were performed.Moreover,synapse microstructure was observed by transmission electron microscopy.At the early stage(6–12 hours after hypoxic ischemia)of hypoxic ischemic injury,expression of glutamate transporter excitatory amino acid transporter-2 and synaptophysin was downregulated,the number of synaptic vesicles was reduced,and synaptic swelling was observed;at 12–24 hours after hypoxic ischemia,the Notch pathway was activated,excitatory amino acid transporter-2 and synaptophysin expression was increased,and the number of synaptic vesicles was slightly increased.Excitatory amino acid transporter-2 and synaptophysin expression decreased after treatment with the Notch pathway inhibitor.This suggests that glutamate transport in astrocytes-neurons after hypoxic ischemic injury is regulated by the Notch pathway and affects vesicle release and synaptic plasticity through the expression of synaptophysin.
基金supported by the Key Research Program of Frontier Sciences,CAS (Grant No. ZDBS-LY-DQC010)the National Natural Science Foundation of China (Grant Nos. 41876012 and 41861144015,42175045)the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No.XDB42000000).
文摘Several consecutive extreme cold events impacted China during the first half of winter 2020/21,breaking the low-temperature records in many cities.How to make accurate climate predictions of extreme cold events is still an urgent issue.The synergistic effect of the warm Arctic and cold tropical Pacific has been demonstrated to intensify the intrusions of cold air from polar regions into middle-high latitudes,further influencing the cold conditions in China.However,climate models failed to predict these two ocean environments at expected lead times.Most seasonal climate forecasts only predicted the 2020/21 La Niña after the signal had already become apparent and significantly underestimated the observed Arctic sea ice loss in autumn 2020 with a 1-2 month advancement.In this work,the corresponding physical factors that may help improve the accuracy of seasonal climate predictions are further explored.For the 2020/21 La Niña prediction,through sensitivity experiments involving different atmospheric-oceanic initial conditions,the predominant southeasterly wind anomalies over the equatorial Pacific in spring of 2020 are diagnosed to play an irreplaceable role in triggering this cold event.A reasonable inclusion of atmospheric surface winds into the initialization will help the model predict La Niña development from the early spring of 2020.For predicting the Arctic sea ice loss in autumn 2020,an anomalously cyclonic circulation from the central Arctic Ocean predicted by the model,which swept abnormally hot air over Siberia into the Arctic Ocean,is recognized as an important contributor to successfully predicting the minimum Arctic sea ice extent.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 12104124 and 12274111)the Natural Science Foundation of Hebei Province, China (Grant Nos. A2021201001 and A2021201008)+4 种基金the Central Guidance Fund on the Local Science and Technology Development of Hebei Province, China (Grant No. 236Z0601G)the Post-graduate’s Innovation Fund Project of Hebei Province, China (Grant No. CXZZSS2023007)the Advanced Talents Incubation Program of the Hebei University, China (Grant Nos. 521000981395, 521000981423, 521000981394, and 521000981390)the Research Foundation of Chongqing University of Science and technology, China (Grant No. ckrc2019017)the High-Performance Computing Center of Hebei University, China。
文摘Skyrmion bags are spin structures with arbitrary topological charges, each of which is composed of a big skyrmion and several small skyrmions. In this work, by using an in-plane alternating current(AC) magnetic field, we investigate the spinwave modes of skyrmion bags, which behave differently from the clockwise(CW) rotation mode and the counterclockwise(CCW) rotation mode of skyrmions because of their complex spin topological structures. The in-plane excitation power spectral density shows that each skyrmion bag possesses four resonance frequencies. By further studying the spin dynamics of a skyrmion bag at each resonance frequency, the four spin-wave modes, i.e., a CCW-CW mode, two CW-breathing modes with different resonance strengths, and an inner CCW mode, appear as a composition mode of outer skyrmion–inner skyrmions. Our results are helpful in understanding the in-plane spin excitation of skyrmion bags, which may contribute to the characterization and detection of skyrmion bags, as well as the applications in logic devices.
基金financially supported by the National Science Fund for Excellent Young Scholars(No.22222813)the National Natural Science Foundation of China(No.22078338)+1 种基金the Key Scientific and Technological Projects in Huizhou(No.2021JBZ5.1)the Joint Fund of Yulin University and the Dalian National Laboratory for Clean Energy(No.YLU-DNL Fund2021016)。
文摘Selective oxidation of alkanes to produce highvalue chemicals is an essential strategy and means to realize efficient utilization of resources.In this work,a strategy of lanthanum manganese mixed metal oxides(LMMO)regulated via a facile ionic liquid(IL)-assisted hydrothermal method was proposed to construct the multifunctional catalysts,which exhibited excellent catalytic performance in the selective aerobic oxidation of cyclohexane.An 8.9%cyclohexane conversion with 90%KA oi(cyclohexanol and cyclohexanone)selectivity was achieved over the optimal LMMO catalyst under mild conditions.The effects of anion type,carbon chain length and concentration of ILs on the structure and properties of catalysts were investigated through various characterizations,indicating the structure-directing and template effect of ILs on the multifunctional catalysts.The formation of self-assembled spherical nanoparticles followed the"dissolution-nucleation-proliferation"mechanism with the introduction of 1-butyl-3-methylimidazolium hydrogen sulfate,ascribing the synergistic effect between the microenvironment of ILs and the hydrothermal environment.Importantly,the high reactive oxygen concentration redox capacity,and suitable basic sites of LMMO catalysts mediated by ILs enhance the activation of C-H bonds and molecular oxygen,simultaneously influencing the adsorption and desorption of the substrate.A comprehensive understanding of the high KA oil selectivity and radical reaction mechanism was elucidated based on in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS)and radical trapping experiments.The recycling and regeneration experiments further illuminated that the removal of adsorbed cyclohexanone acting on the LMMO catalyst was the key to achieve high KA oil selectivity.
文摘BACKGROUND Penetrating neck injuries require prompt recognition,diagnosis and management of critical airways.This case demonstrates an emergent situation that a“medical negligence”was avoided with the aid of end-tidal carbon dioxide(ETCO2)waveform.CASE SUMMARY We report a case of malposition of the endotracheal tube into the right hemithoracic cavity for cervical knife trauma,resulting in pneumothorax.Tube placement was not confirmed during emergency airway management,and the patient was directly transferred to the emergency operation room.Assisted by ETCO2 and imaging examinations,the anesthetist timely noticed the absence of ETCO2 waveform and resolved this urgent situation before anesthesia induction.CONCLUSION This case emphasizes the necessity of ETCO2 waveform and/or X-ray confirmation of endotracheal intubation even in emergent situations.
文摘Ferulic acid(FA)is a phenolic phytochemical with antioxidant and anti-inflammatory pharmacological effects.In recent years,the neuroprotective effects of FA have been studied extensively.Many researchers have attempted to use FA to prevent and treat neurological diseases and have made some progress.This paper mainly collated the study of the protective effect of FA on stroke and summarized the protective effect of FA on ischemic stroke and the potential protective effects of FA on neurovascular units.
基金supported by grants from National Funds for Distinguished Young Scientists of China (81225007)the Funds for Creative Research Groups of China (81221003)
文摘Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recording in various ex vivo methods. However, it is difficult to maintain the activity and morphology of neurons in slices from mice of 〉30 days old. Here we evaluated the N-methyI-D-glucamine （NMDG）- based artificial cerebrospinal fluid （aCSF） method for the preservation of interneurons in slices from mice of up to -6 months old and discussed the steps that may affect their quality during slicing. We found that the NMDG-aCSF method rescued more cells than sucrose-aCSF and successfully preserved different types of interneurons including parvalbumin- and somatostatin-positive interneurons. In addition, both the chemical and electrical synaptic signaling of interneurons were maintained. These results demonstrate that the NMDG-aCSF method is suitable for the preservation of interneurons, especially in studies of gap junctions.
基金supported by the National Natural Science Foundation of China[No.H0517/81560132 and No.81970583]the Supporting Project for the Foregoers of Main Disciplines of Jiangxi Province[No.20162BCB22023].
文摘Background:Observational studies suggest that hepatorenal syndrome(HRS)patients who receive pharmacological therapy before orthotopic liver transplantation display a post-transplant outcome similar to those without HRS.The aim of this study was to comprehensively compare and rank the pharmacological therapies for HRS.Methods:We reviewed PubMed,Elsevier,Medline,and the Cochrane Central Register of Controlled Trials(CENTRAL)for studies that were published between 1 January 1999 and 24 February 2018.The primary endpoint was reversal of HRS.The secondary endpoints were the changes in serum creatinine(Scr)and serum sodium.We evaluated the different therapeutic strategies using network meta-analysis on the basis of Bayesian methodology.Results:The study included 24 articles with 1,419 participants evaluating seven different therapeutic strategies for HRS.The most effective treatments to induce reversal of HRS were terlipressin plus albumin,noradrenaline plus albumin,and terlipressin,which had a surface under the cumulative ranking curve(SUCRA)of 0.086,0.151,and 0.451,respectively.The top two treatments for decreasing Scr were dopamine plus furosemide plus albumin(rank probability:0.620)and terlipressin plus albumin(rank probability:0.570).For increasing serum sodium,the optimal treatment was octreotide plus midodrine plus albumin(rank probability:0.800),followed by terlipressin plus albumin(rank probability:0.544).Conclusions:Terlipressin plus albumin and dopamine plus furosemide plus albumin should be prioritized for decreasing Scr in HRS,and octreotide plus midodrine plus albumin was the most effective at increasing serum sodium.Terlipressin plus albumin showed a comprehensive effect in both decreasing Scr and increasing serum sodium.
