The Communist Manifesto,as the initial comprehensive exposition of scientific socialist theory,encapsulates the scientific spirit of Marxism.As socialism with Chinese characteristics enters a new epoch,and the world i...The Communist Manifesto,as the initial comprehensive exposition of scientific socialist theory,encapsulates the scientific spirit of Marxism.As socialism with Chinese characteristics enters a new epoch,and the world is in the throes of an unprecedented century-long transformation,the importance of young talent in bolstering national scientific and technological progress grows ever more apparent.It is of paramount significance to profoundly understand and elucidate the theoretical heritage,practical guidance,and directional leadership that The Communist Manifesto provides to scientific education for the young talent of this new era from three perspectives:theory,practice,and history.This understanding is vital for young talents to further deepen their accurate comprehension of the governing laws of the Communist Party,the construction laws of socialism,and the development laws of human society.The nurturing of scientific spirit and scientific literacy in young talents has critical implications for providing talent support in building a world scientific and technological powerhouse.展开更多
The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific ...The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcriptome of A. pectinata was deeply sequenced using Illumina pair-end sequencing technology. After assembling, a total of 127263 unigenes were obtained. Functional annotation indicated that the highest percentage of unigenes(18.60%) was annotated on GO database, followed by 18.44% on PFAM database and 17.04% on NR database. There were 270 biological pathways matched with those in KEGG database. Furthermore, a total of 23452 potential simple sequence repeats(SSRs) were identified, of them the most abundant type was mono-nucleotide repeats(12902, 55.01%), which was followed by di-nucleotide(8132, 34.68%), tri-nucleotide(2010, 8.57%), tetra-nucleotide(401, 1.71%), and penta-nucleotide(7, 0.03%) repeats. Sixty SSRs were selected for validating and developing genic SSR markers, of them 23 showed polymorphism in a cultured population with the average observed and expected heterozygosities of 0.412 and 0.579, respectively. In this study, we established the first comprehensive transcript dataset of A. pectinata genes. Our results demonstrated that RNA-Seq is a fast and cost-effective method for genic SSR development in non-model species.展开更多
文摘The Communist Manifesto,as the initial comprehensive exposition of scientific socialist theory,encapsulates the scientific spirit of Marxism.As socialism with Chinese characteristics enters a new epoch,and the world is in the throes of an unprecedented century-long transformation,the importance of young talent in bolstering national scientific and technological progress grows ever more apparent.It is of paramount significance to profoundly understand and elucidate the theoretical heritage,practical guidance,and directional leadership that The Communist Manifesto provides to scientific education for the young talent of this new era from three perspectives:theory,practice,and history.This understanding is vital for young talents to further deepen their accurate comprehension of the governing laws of the Communist Party,the construction laws of socialism,and the development laws of human society.The nurturing of scientific spirit and scientific literacy in young talents has critical implications for providing talent support in building a world scientific and technological powerhouse.
基金the grants from Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, P. R. China (No. 2016LMFS-B02)the Key Research and Development Program of Shandong Province (No. 2016GSF115012)+1 种基金Basic Scientific Research Fund of YSFRI (No. 2060302201516054)Natural Science Foundation of Shandong Province (No. ZR2016 CQ32)
文摘The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcriptome of A. pectinata was deeply sequenced using Illumina pair-end sequencing technology. After assembling, a total of 127263 unigenes were obtained. Functional annotation indicated that the highest percentage of unigenes(18.60%) was annotated on GO database, followed by 18.44% on PFAM database and 17.04% on NR database. There were 270 biological pathways matched with those in KEGG database. Furthermore, a total of 23452 potential simple sequence repeats(SSRs) were identified, of them the most abundant type was mono-nucleotide repeats(12902, 55.01%), which was followed by di-nucleotide(8132, 34.68%), tri-nucleotide(2010, 8.57%), tetra-nucleotide(401, 1.71%), and penta-nucleotide(7, 0.03%) repeats. Sixty SSRs were selected for validating and developing genic SSR markers, of them 23 showed polymorphism in a cultured population with the average observed and expected heterozygosities of 0.412 and 0.579, respectively. In this study, we established the first comprehensive transcript dataset of A. pectinata genes. Our results demonstrated that RNA-Seq is a fast and cost-effective method for genic SSR development in non-model species.
