Heterojunction catalysts composed of transition metal sulfides exhibited excellent potentials in electrocatalytic water splitting.Herein,we have designed a FeS/Co_(3)S_(4)heterojunction catalyst for hydrogen evolution...Heterojunction catalysts composed of transition metal sulfides exhibited excellent potentials in electrocatalytic water splitting.Herein,we have designed a FeS/Co_(3)S_(4)heterojunction catalyst for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)in alkaline water/seawater solution.Three-dimensional nanoarrays were grown on nickel foam,and the successful synthesis of heterojunction endowed excellent activity to the catalyst.In alkaline water/seawater solution,the low overpotentials(at current density of 10 mA/cm^(2))of HER were 120.3 and 135.6 mV and the low overpotentials of OER were 212 and 232 mV,respectively.This work provided an effective method for highly-efficiently electrocatalytic splitting of water via fabrication of heterojunction.展开更多
In order to control the quality of spline shaft in rolling process, an efficient measurement method for rolling performance evaluation is essential. Here, a newly developed on-machine non-contact measurement prototype...In order to control the quality of spline shaft in rolling process, an efficient measurement method for rolling performance evaluation is essential. Here, a newly developed on-machine non-contact measurement prototype based on laser displacement sensor and rotary encoder is proposed. The prototype is intended for the automated evaluation of the spline shaft rolling performance by measuring the dimensional change of tooth root, which is correlated with the surface residual stress and micro-hardness. Laser displacement sensor and rotary encoder are used to record the polar radius and polar angle of each point on measuring section. Data are displayed in a polar coordinate system and fitted in a gear. Through multipoint curvature method, the roots of spline shaft are recognized automatically. Then, the dimensional change can be calculated by fitting the radius of the tooth root circle before and after rolling. Systematic error covering offset error is also analyzed and calibrated. At last, measurement test results show that the system has advantages of simple structure, high measurement precision(radius error < 0.6 μm), high measurement efficiency(measuring time < 2 s) and automatic control ability, providing a new opportunity for the efficient evaluation of various spline shafts in high-precision mechanical processing.展开更多
The chemical and physical characteristics of PM2.5, especially their temporal and geographical variations, have been explored in metropolitan Hangzhou area (China) by a field campaign from September 2010 to July 2011....The chemical and physical characteristics of PM2.5, especially their temporal and geographical variations, have been explored in metropolitan Hangzhou area (China) by a field campaign from September 2010 to July 2011. Annual average concentrations of PM2.5 and PM10 during non-raining days were 106 - 131 μg.m-3 and 127 - 158 μg.m-3, respectively, at three stations in urban breathing zones, while corresponding concentrations of PM2.5 and PM10 at an urban background station (16 mabove ground level in a park) were 78 and 104 μg.m-3, respectively. For comparison, the annual average PM10 concentration at a suburban station (5 mAGL) was 93 μg.m-3. Detailed chemical analyses were also conducted for all samples collected during the campaign. We found that toxic metals (Cd, As, Pb, Zn, Mo, Cu, Hg) were highly enriched in the breathing zones due to anthropogenic activities, while soluble ions (, , ) and total carbon accounted for majority of PM2.5 mass. Unlike most areas in China where sulfate was several times of nitrate in fine PM, nitrate was as important as sulfate and highly correlated with ammonium during the campaign. Thus, a historical shift from sulfate-dominant fine PM to nitrate-dominant fine PM was documented.展开更多
Cystine is the primary source material for the synthesis of glutathione.However,the pharmacokinetics and tissue distribution of cystine are largely unknown.A surrogate analyte D_(4)-cystine was employed to generate ca...Cystine is the primary source material for the synthesis of glutathione.However,the pharmacokinetics and tissue distribution of cystine are largely unknown.A surrogate analyte D_(4)-cystine was employed to generate calibration curves for the determination of levels of D_(4)-cystine and endogenous cystine in mice by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Validation assessments proved the sensitivity,specificity and reproducibility of the method with a lower limit of quantification(LLOQ)of 5 ng/mL over 5e5000 ng/mL in plasma.The pharmacokinetics of D_(4)-cystine were evaluated after administering injections and oral solutions,both of which minimally impacted endogenous cystine levels.The absolute bioavailability of cystine was 18.6%,15.1%and 25.6%at doses of 25,50 and 100 mg/kg,respectively.Intravenously injected D_(4)-cystine resulted in dramatically high plasma levels with reduced levels in the brain and liver.Intragastrically administered D_(4)-cystine resulted in high levels in the plasma and stomach with relatively low levels in the lung,kidney,heart and brain.展开更多
As a new method of literary criticism raised by Chinese scholars,ethical literary criticism has created a theoretical system with Chinese characteristics and become the successful"going global"model of Chine...As a new method of literary criticism raised by Chinese scholars,ethical literary criticism has created a theoretical system with Chinese characteristics and become the successful"going global"model of Chinese academics by standing on the Chinese context and adhering to the spirit of not forgetting the origin,absorbing the foreign,and facing the future through more than10 years of advocacy and practice.This paper analyzes and summarizes the inheritance,nationality,integration and innovation of the theory of literary ethical criticism on the basis of combing the development history of ethical literary criticism at home and abroad,and then analyzes four important reasons why ethical literary criticism can successfully represent the"going global"of Chinese academics.This paper also discusses the current situation,problems,solutions,trend and future of ethical literary criticism,and points out that ethical literary criticism provides model and reference for realizing the grand blueprint of the"going global"of Chinese academics.展开更多
[ Objective] To develop an indirect ELISA assay for detecting antibodies against envelope glycoprotein （ E protein） of Japanese encephalitis virus （JEV）. [ Method] Specific primers were designed according to JEV s...[ Objective] To develop an indirect ELISA assay for detecting antibodies against envelope glycoprotein （ E protein） of Japanese encephalitis virus （JEV）. [ Method] Specific primers were designed according to JEV sequences published in the GenBank. The cDNA of JEV E gene （about 1 000 10p） was amplified by the RT-PCR with the specific primers. After sequencing analysis, the E gene was cloned into pET30a expression vector and expressed in E. coli BL21 （DE3） with the induction of IPTG. After denaturation, purification and renaturation, the recombinant protein was analyzed by the SDS-PAGE and the westem blotting. An indirect ELISA was developed to detect antibodies against JEV. [ Result] The E protein was mainly expressed in inclusion body. With the purified E protein, the indirect ELISA was developed and displayed good specificity, sensitivity and repeatability, [ Conclusion]The developed ELISA using the truncated E protein as antigen is a simple, convenient and rapid serological method for diagnosis, monitoring antibody level and epidemiological investigation of JEV.展开更多
The purpose of this study was to develop a method for detecting antibodies against porcine pseudorabies virus （PRV）. According to the published genomic sequence of PRV SA strain, an approximately 1 070-bp gD gene fr...The purpose of this study was to develop a method for detecting antibodies against porcine pseudorabies virus （PRV）. According to the published genomic sequence of PRV SA strain, an approximately 1 070-bp gD gene fragment was amplified by PCR. The PCR products were cloned into the prokaryotic expression vector pET30a and the positive recombinant plasmid was transformed into E. coli BL21. Through induction with IPTG, the recombinant gD protein was expressed as inclusion bodies. As analyzed by western blot assay, the purified recombinant gD protein had good antigenicity and high specificity. Using the purified gD protein as coating antigen and horseradish peroxidase labeled staphylococcal protein A （PPA） as secondary antibody, we developed a PPA-ELISA for detecting antibodies against porcine PPV. No cross-reaction with the positive sera against seven common pathogens in pigs including classical swine fever virus, porcine parvovirus, porcine reproductive and respiratory syndrome, Japanese encephalitis virus, porcine circovirus type 2, porcine epidemic diarrhea virus, transmissible gastroenteritis virus was observed. The repeatability test showed that the intra- and inter-assay coefficients of variation were lower than 5% and 10%, respectively. Compared with the ELISA gD antibody test kit produced by IDEXX, the coincidence, sensitivity and specificity of the developed PPA-ELISA were 92.0%, 95.1% and 88.1%, respectively. The developed PPA-ELISA had good repeatability, sensitivity and specificity and was a rapid and simple serological method for surveillance of PRV antibodies in pig herds as well as for rapid diagnosis and epidemiological investigation of PRV infection.展开更多
基金supported by the Dean/Opening Project of Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology(2023K001)Taishan Scholars Foundation of Shandong province(TSQN201909058).
文摘Heterojunction catalysts composed of transition metal sulfides exhibited excellent potentials in electrocatalytic water splitting.Herein,we have designed a FeS/Co_(3)S_(4)heterojunction catalyst for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)in alkaline water/seawater solution.Three-dimensional nanoarrays were grown on nickel foam,and the successful synthesis of heterojunction endowed excellent activity to the catalyst.In alkaline water/seawater solution,the low overpotentials(at current density of 10 mA/cm^(2))of HER were 120.3 and 135.6 mV and the low overpotentials of OER were 212 and 232 mV,respectively.This work provided an effective method for highly-efficiently electrocatalytic splitting of water via fabrication of heterojunction.
基金Supported by Industrial Technology Development Program of China(Grant Nos.JCKY2017208C005,A0920132008)National Natural Science Foundation of China(Grant No.51575049)
文摘In order to control the quality of spline shaft in rolling process, an efficient measurement method for rolling performance evaluation is essential. Here, a newly developed on-machine non-contact measurement prototype based on laser displacement sensor and rotary encoder is proposed. The prototype is intended for the automated evaluation of the spline shaft rolling performance by measuring the dimensional change of tooth root, which is correlated with the surface residual stress and micro-hardness. Laser displacement sensor and rotary encoder are used to record the polar radius and polar angle of each point on measuring section. Data are displayed in a polar coordinate system and fitted in a gear. Through multipoint curvature method, the roots of spline shaft are recognized automatically. Then, the dimensional change can be calculated by fitting the radius of the tooth root circle before and after rolling. Systematic error covering offset error is also analyzed and calibrated. At last, measurement test results show that the system has advantages of simple structure, high measurement precision(radius error < 0.6 μm), high measurement efficiency(measuring time < 2 s) and automatic control ability, providing a new opportunity for the efficient evaluation of various spline shafts in high-precision mechanical processing.
文摘The chemical and physical characteristics of PM2.5, especially their temporal and geographical variations, have been explored in metropolitan Hangzhou area (China) by a field campaign from September 2010 to July 2011. Annual average concentrations of PM2.5 and PM10 during non-raining days were 106 - 131 μg.m-3 and 127 - 158 μg.m-3, respectively, at three stations in urban breathing zones, while corresponding concentrations of PM2.5 and PM10 at an urban background station (16 mabove ground level in a park) were 78 and 104 μg.m-3, respectively. For comparison, the annual average PM10 concentration at a suburban station (5 mAGL) was 93 μg.m-3. Detailed chemical analyses were also conducted for all samples collected during the campaign. We found that toxic metals (Cd, As, Pb, Zn, Mo, Cu, Hg) were highly enriched in the breathing zones due to anthropogenic activities, while soluble ions (, , ) and total carbon accounted for majority of PM2.5 mass. Unlike most areas in China where sulfate was several times of nitrate in fine PM, nitrate was as important as sulfate and highly correlated with ammonium during the campaign. Thus, a historical shift from sulfate-dominant fine PM to nitrate-dominant fine PM was documented.
基金This study was financially supported by the National Natural Science Foundation of the People's Republic of China(Grant Nos.:81773814 and 81530098)the National Key Special Project of Science and Technology for Innovation Drugs of China(Project No.:2017ZX09301013)the National Key Research and Development Program(Grant No.:2018YFC0807403).
文摘Cystine is the primary source material for the synthesis of glutathione.However,the pharmacokinetics and tissue distribution of cystine are largely unknown.A surrogate analyte D_(4)-cystine was employed to generate calibration curves for the determination of levels of D_(4)-cystine and endogenous cystine in mice by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Validation assessments proved the sensitivity,specificity and reproducibility of the method with a lower limit of quantification(LLOQ)of 5 ng/mL over 5e5000 ng/mL in plasma.The pharmacokinetics of D_(4)-cystine were evaluated after administering injections and oral solutions,both of which minimally impacted endogenous cystine levels.The absolute bioavailability of cystine was 18.6%,15.1%and 25.6%at doses of 25,50 and 100 mg/kg,respectively.Intravenously injected D_(4)-cystine resulted in dramatically high plasma levels with reduced levels in the brain and liver.Intragastrically administered D_(4)-cystine resulted in high levels in the plasma and stomach with relatively low levels in the lung,kidney,heart and brain.
基金Social Science Fund Project of Jangsu Provinece in 2017：Research on the Marriage and Family Ethics in Natsume Soseki’s Literature(Project No.：17WWD003)Universities’ Philosophy and Social Science Researche of Jiangsu Province in 2016：Critical Research on
文摘As a new method of literary criticism raised by Chinese scholars,ethical literary criticism has created a theoretical system with Chinese characteristics and become the successful"going global"model of Chinese academics by standing on the Chinese context and adhering to the spirit of not forgetting the origin,absorbing the foreign,and facing the future through more than10 years of advocacy and practice.This paper analyzes and summarizes the inheritance,nationality,integration and innovation of the theory of literary ethical criticism on the basis of combing the development history of ethical literary criticism at home and abroad,and then analyzes four important reasons why ethical literary criticism can successfully represent the"going global"of Chinese academics.This paper also discusses the current situation,problems,solutions,trend and future of ethical literary criticism,and points out that ethical literary criticism provides model and reference for realizing the grand blueprint of the"going global"of Chinese academics.
文摘[ Objective] To develop an indirect ELISA assay for detecting antibodies against envelope glycoprotein （ E protein） of Japanese encephalitis virus （JEV）. [ Method] Specific primers were designed according to JEV sequences published in the GenBank. The cDNA of JEV E gene （about 1 000 10p） was amplified by the RT-PCR with the specific primers. After sequencing analysis, the E gene was cloned into pET30a expression vector and expressed in E. coli BL21 （DE3） with the induction of IPTG. After denaturation, purification and renaturation, the recombinant protein was analyzed by the SDS-PAGE and the westem blotting. An indirect ELISA was developed to detect antibodies against JEV. [ Result] The E protein was mainly expressed in inclusion body. With the purified E protein, the indirect ELISA was developed and displayed good specificity, sensitivity and repeatability, [ Conclusion]The developed ELISA using the truncated E protein as antigen is a simple, convenient and rapid serological method for diagnosis, monitoring antibody level and epidemiological investigation of JEV.
基金funded by the Special Fund for Research and Development of Application Technology of Binzhou City(200706)Youth Science and Technology Innovation Fund of Shandong Binzhou Animal Science & Veterinary Medicine Academy (2007-02)
文摘The purpose of this study was to develop a method for detecting antibodies against porcine pseudorabies virus （PRV）. According to the published genomic sequence of PRV SA strain, an approximately 1 070-bp gD gene fragment was amplified by PCR. The PCR products were cloned into the prokaryotic expression vector pET30a and the positive recombinant plasmid was transformed into E. coli BL21. Through induction with IPTG, the recombinant gD protein was expressed as inclusion bodies. As analyzed by western blot assay, the purified recombinant gD protein had good antigenicity and high specificity. Using the purified gD protein as coating antigen and horseradish peroxidase labeled staphylococcal protein A （PPA） as secondary antibody, we developed a PPA-ELISA for detecting antibodies against porcine PPV. No cross-reaction with the positive sera against seven common pathogens in pigs including classical swine fever virus, porcine parvovirus, porcine reproductive and respiratory syndrome, Japanese encephalitis virus, porcine circovirus type 2, porcine epidemic diarrhea virus, transmissible gastroenteritis virus was observed. The repeatability test showed that the intra- and inter-assay coefficients of variation were lower than 5% and 10%, respectively. Compared with the ELISA gD antibody test kit produced by IDEXX, the coincidence, sensitivity and specificity of the developed PPA-ELISA were 92.0%, 95.1% and 88.1%, respectively. The developed PPA-ELISA had good repeatability, sensitivity and specificity and was a rapid and simple serological method for surveillance of PRV antibodies in pig herds as well as for rapid diagnosis and epidemiological investigation of PRV infection.
