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miR-19b-3p靶向IGF-1参与绒毛膜癌发生发展的机制研究
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作者 陈光元 李日红 邓闹 《中国现代药物应用》 2024年第11期176-180,共5页
目的探究miR-19b-3p靶向胰岛素样生长因子-1(IGF-1)参与绒毛膜癌增殖、侵袭和转移的机制。方法以人绒毛膜癌细胞系JEG-3为研究对象,A组:miR-19b-3p mimics组,B组:miR-19b-3p mimics NC组,C组:miR-19b-3p inhibitor组,D组:miR-19b-3p inh... 目的探究miR-19b-3p靶向胰岛素样生长因子-1(IGF-1)参与绒毛膜癌增殖、侵袭和转移的机制。方法以人绒毛膜癌细胞系JEG-3为研究对象,A组:miR-19b-3p mimics组,B组:miR-19b-3p mimics NC组,C组:miR-19b-3p inhibitor组,D组:miR-19b-3p inhibitor NC组,E组:空白对照组。A组、B组、C组、D组按照分组情况进行相应转染,E组不做任何处理。分别用Real-time PCR和Western blot检测五组miR-19b-3p RNA和IGF-1蛋白表达水平,采用CCK-8法测定细胞增殖,采用划痕实验和Transwell小室侵袭实验测定细胞迁移和侵袭能力,并进行比较;采用双荧光素酶(Dualluciferase)报告实验验证miR-19b-3p对IGF-1的靶向关系。结果与E组的(0.98±0.13)相比,A组miR-19b-3p mRNA表达水平(2.23±0.16)明显上调,C组miR-19b-3p mRNA表达水平(0.73±0.08)明显下调(P<0.05)。与E组的(1.03±0.03)nmol/L相比,A组IGF-1蛋白表达水平(1.48±0.12)nmol/L明显上调,C组IGF-1蛋白表达水平(0.76±0.05)nmol/L明显下调(P<0.05)。72 h时,与E组的(5.03±0.14)%相比,A组细胞增殖活力(10.34±0.87)%明显增加,C组细胞增殖活力(2.42±0.13)%明显下调(P<0.05);与E组的(1.09±0.07)mm相比,A组细胞划痕迁移距离(1.45±0.08)mm明显增加,C组细胞划痕迁移距离(0.73±0.07)mm明显减少(P<0.05)。与E组的(103.00±7.00)个相比,A组侵袭细胞数(173.00±4.00)个明显增加,C组侵袭细胞数(82.00±1.00)个明显减少(P<0.05)。双荧光素酶报告实验结果显示:miR-19b-3p转染WT-IGF-1的细胞荧光素酶活性(0.57±0.08)Kat低于miR-NC的(0.95±0.06)Kat(P<0.05);miR-19b-3p转染MUT-IGF-1的细胞荧光素酶活性(0.98±0.05)Kat与miR-NC的(0.96±0.06)Kat比较无明显差异(P>0.05)。结论miR-19b-3p靶向上调IGF-1促进绒毛膜癌JEG-3细胞增殖、侵袭、迁移。 展开更多
关键词 绒毛膜癌 miR-19b-3p 胰岛素样生长因子-1 增殖 侵袭
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Input characteristics and risk analysis of ballast water from offshore entry ships in China 被引量:2
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作者 DU Huan ZHANG Xiao-fang +2 位作者 ZHANG Zhi-tao liU Kai-ying li ri-hong 《Marine Science Bulletin》 CAS 2017年第2期20-37,共18页
Large quantities of ballast water discharge from ocean going ships in sea ports of China is one of the important factorswhich cause the spread of aquatic nonindigenous harmful species isolated geographically by waters... Large quantities of ballast water discharge from ocean going ships in sea ports of China is one of the important factorswhich cause the spread of aquatic nonindigenous harmful species isolated geographically by waters,the deteriorating environment of the near-shore water area and the frequent outbreaks of red tides.In this paper,the total amount of the ballast water input estimation model for entry ships in Chinese ports was established.The information of foreign trade shipping and the import and export goods released publicly by the State Department of Transportation and the State General Administration of Customs were investigated.And then,the input features and its ecological environment risk of ballast water in China's offshore entry ships from2007to2012were analyzed based on the established total input amounts of ballast water from entry ships to Chinese sea ports together with the ballast water input ratio of the five major port-groups in China.The results show that:the total ballast water input amounts from entry ships of the five major port-groups in China are extremely imbalanced.The most developed Yangtze River Delta in economy has the biggest total ballast water input amounts,103.61million tons in2012.The second is the Circum-Bohai Sea Region(73.66million tons)and the third is the Pearl River Delta(67.24million tons).The total ballast water input amounts of the northwest and the southwest coastal areas are less,only16.57and5.71million tons respectively.The large quantity of entry ships’ballast water discharge has been an enormous threat to ecological environment of our country's sea areas,especially to economically developed regions. 展开更多
关键词 ballast water input of ballast water marine environment and ecology risk analysis
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