Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but ha...Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by PHWD.Compared to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose accumulation.Increased sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline contents.An abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under PHWD.Yeast one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive elements.When Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased correspondingly.Further,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs SUT1.Overall,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to PHWD.Our results provide a detail insight into the quality formation of citrus fruit.展开更多
Resection of oral and maxillofacial tumors is often accompanied by the inferior alveolar nerve neurectomy,resulting in abnormal sensation in lower lip.It is generally believed that spontaneous sensory recovery in this...Resection of oral and maxillofacial tumors is often accompanied by the inferior alveolar nerve neurectomy,resulting in abnormal sensation in lower lip.It is generally believed that spontaneous sensory recovery in this nerve injury is difficult.However,during our follow-up,patients with inferior alveolar nerve sacrifice showed different degrees of lower lip sensory recovery.In this study,a prospective cohort study was conducted to demonstrate this phenomenon and analyze the factors influencing sensory recovery.展开更多
Lacunar stroke is a cerebral small vessel disease that accounts for nearly 25%of cases of ischemic stroke.As an age-related disease,the incidence of lacunar stroke increases with age.Generally,biological age is equal ...Lacunar stroke is a cerebral small vessel disease that accounts for nearly 25%of cases of ischemic stroke.As an age-related disease,the incidence of lacunar stroke increases with age.Generally,biological age is equal to chronological age.However,under the influence of a range of chronic diseases and circumstances,the biological age can accelerate,which may contribute to the variation in risk of illness and death between individuals.展开更多
目的 检索、总结宫颈癌患者术后性健康教育有关的最佳证据,为更好地对临床宫颈癌患者提供性健康和功能障碍方面的宣教奠定基础。方法 以“6S”金字塔为模型,使用计算机检索BMJ Best Practice、Up To Date、国际指南协作网、美国国立指...目的 检索、总结宫颈癌患者术后性健康教育有关的最佳证据,为更好地对临床宫颈癌患者提供性健康和功能障碍方面的宣教奠定基础。方法 以“6S”金字塔为模型,使用计算机检索BMJ Best Practice、Up To Date、国际指南协作网、美国国立指南库、英国国家临床医学研究所指南库、苏格兰学院间指南网等关于宫颈癌患者性健康教育相关的指南(检索时限为建库至2022年9月20日),并检索了性专业网站及肿瘤相关网站。根据纳入标准遴选文献,由2名研究者背对背对纳入文献进行质量评价和证据提取。结果 本研究最终共纳入5篇指南,形成的证据包括5个方面(分别为评估问询、相关症状、性心理、阴道扩张和用药指导)共20条证据。结论 宫颈癌患者术后性健康教育的最佳证据总结,有助于指导医护人员以更科学的方式对宫颈癌患者进行健康宣教,从而改善患者的生活质量和心理幸福感。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32172520)the earmarked fund for China Agriculture Research System(Grant No.CARS-26)。
文摘Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by PHWD.Compared to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose accumulation.Increased sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline contents.An abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under PHWD.Yeast one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive elements.When Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased correspondingly.Further,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs SUT1.Overall,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to PHWD.Our results provide a detail insight into the quality formation of citrus fruit.
基金supported by the Applied Basic Research Programs of the Sichuan Provincial Science and Technology Department(Grant number:2021YJ0129)Research Program of Science and Technology Department of Sichuan Province(Grant number:2021YFS0217)the National Natural Science Foundation of China(81870782)。
文摘Resection of oral and maxillofacial tumors is often accompanied by the inferior alveolar nerve neurectomy,resulting in abnormal sensation in lower lip.It is generally believed that spontaneous sensory recovery in this nerve injury is difficult.However,during our follow-up,patients with inferior alveolar nerve sacrifice showed different degrees of lower lip sensory recovery.In this study,a prospective cohort study was conducted to demonstrate this phenomenon and analyze the factors influencing sensory recovery.
基金supported by the National Natural Science Foundation of China[81971116]the Shaanxi Provincial Key Research and Development Project of China[2019ZDLSF01–04]。
文摘Lacunar stroke is a cerebral small vessel disease that accounts for nearly 25%of cases of ischemic stroke.As an age-related disease,the incidence of lacunar stroke increases with age.Generally,biological age is equal to chronological age.However,under the influence of a range of chronic diseases and circumstances,the biological age can accelerate,which may contribute to the variation in risk of illness and death between individuals.
文摘目的 检索、总结宫颈癌患者术后性健康教育有关的最佳证据,为更好地对临床宫颈癌患者提供性健康和功能障碍方面的宣教奠定基础。方法 以“6S”金字塔为模型,使用计算机检索BMJ Best Practice、Up To Date、国际指南协作网、美国国立指南库、英国国家临床医学研究所指南库、苏格兰学院间指南网等关于宫颈癌患者性健康教育相关的指南(检索时限为建库至2022年9月20日),并检索了性专业网站及肿瘤相关网站。根据纳入标准遴选文献,由2名研究者背对背对纳入文献进行质量评价和证据提取。结果 本研究最终共纳入5篇指南,形成的证据包括5个方面(分别为评估问询、相关症状、性心理、阴道扩张和用药指导)共20条证据。结论 宫颈癌患者术后性健康教育的最佳证据总结,有助于指导医护人员以更科学的方式对宫颈癌患者进行健康宣教,从而改善患者的生活质量和心理幸福感。
