The microstructure in the weld metals for HQ130+QJ63 high strength steels, which are welded by Ar CO 2 gas shielded metal arc welding, was analyzed by means of microscope and scan electron microscope (SEM). The rela...The microstructure in the weld metals for HQ130+QJ63 high strength steels, which are welded by Ar CO 2 gas shielded metal arc welding, was analyzed by means of microscope and scan electron microscope (SEM). The relative content of different microstructure was evaluated with XQF 2000 micro image analyzer. The effect of acicular ferrite content on the impact toughness was also studied. The test results indicated that the main microstructure in the weld metals of HQ130+QJ63 high strength steels is acicular ferrite and a few pro eutectic ferrite on the boundary of original austenite grain. Near the fusion zone there are columnar grains whose direction coefficient (X) is 3 22, but the microstructure in the center of the weld metal is isometric grain, whose direction coefficient X=1 In order to avoid welding crack and improve welding technology the weld heat input should be strictly controlled in 10-16 kJ/cm. Thus, the main microstructure in the weld metals is fine acicular ferrite and the content of pro eutectic ferrite is limited. The impact toughness in the weld metals of HQ130+QJ63 steels can be ensured and can meet the requirements for application in engineering and machinery.展开更多
With TEM、SEM, various high temperature deformed structures in W9Mo3Cr4V steel were investigated. The sub structures,recrystallized nuclei, as well as the dynamic precipitation were also studied and analyzed. The r...With TEM、SEM, various high temperature deformed structures in W9Mo3Cr4V steel were investigated. The sub structures,recrystallized nuclei, as well as the dynamic precipitation were also studied and analyzed. The relationship between recrystallized structures and dynamic precipitation was discussed. The results showed that the deformed structures in W9Mo3Cr4V steel are more complicated than those in low alloy steels. Because W9Mo3Cr4V steel is a high speed steel, there are a large number of residual carbides on the matrix. Also, much dynamic precipitating carbides will precipitate during deformation at high temperature.展开更多
OBJECTIVE The present study was designed to investigate anticancer effect of zeylenone(Zey)on K562 cells derived from chronic myelogenous leukemia(CML)both in vitro and in vivo,followed by exploring the underlying mec...OBJECTIVE The present study was designed to investigate anticancer effect of zeylenone(Zey)on K562 cells derived from chronic myelogenous leukemia(CML)both in vitro and in vivo,followed by exploring the underlying mechanisms.METHODS Initially,the effects of Zey on cel viability,proliferation,and apoptosis were measured in K562 cells by MTT,soft agar assay,AO/EB staining,hoechst 33258 staining and flow cytometric analysis after they were treated with Zey for indicated time,the involving signaling pathways were then investigated by JC-1,real-time quantitative polymerase chain reaction(RT-q PCR),Western blotting and immunofluorescence analysis.Furthermore,the in vivo anti-tumoractivity of Zey was assessed with nude xenografts and the involving mechanism was confirmed by immunohistochemical(IHC)and histopathological analysis.RESULTS We identified that Zey dose-dependently decreased cell viability,colony formation and expression of Proliferating Cell Nuclear Antigen(PCNA),and significantly induced K562 cell apoptosis via regulating Bcl-2 family members,decreasing mitochondrial transmembrane potential,and activating caspase-3,caspase-9,and caspase-8(P<0.05 or P<0.01).Further study revealed that Zey significantly inhibited phosphorylation of Jak2 and Src and downregulated their downstream proteins,including stat3,PI3K/AKT/m TOR,and ERK1/2 signaling pathways(P<0.05 or P<0.01).Zey also suppressed tumor growth with low toxicity in mouse xenograft model of K562cells through decreasing expression of Jak2 and Src.CONCLUSION Our data demonstrated that Zey substantially suppressed K562 cells both in vitro and in vivo through Jak2 and Src pathways.These findings suggest the potential of Zey as an effective anticancer agent in CML treatment.展开更多
The application of parametric modeling method of Pro/E in the modular dougong is researched,and the disassembly and assembly operation of the dougong is implemented through the collision detection by the coordinate me...The application of parametric modeling method of Pro/E in the modular dougong is researched,and the disassembly and assembly operation of the dougong is implemented through the collision detection by the coordinate method in VRML. Direct interaction of VRML scene at the Web is implemented by using Java Script to manipulate VRML Object DOM.展开更多
基金Sponsored by National Key Fundamental Research Development Project(G1998061513)
文摘The microstructure in the weld metals for HQ130+QJ63 high strength steels, which are welded by Ar CO 2 gas shielded metal arc welding, was analyzed by means of microscope and scan electron microscope (SEM). The relative content of different microstructure was evaluated with XQF 2000 micro image analyzer. The effect of acicular ferrite content on the impact toughness was also studied. The test results indicated that the main microstructure in the weld metals of HQ130+QJ63 high strength steels is acicular ferrite and a few pro eutectic ferrite on the boundary of original austenite grain. Near the fusion zone there are columnar grains whose direction coefficient (X) is 3 22, but the microstructure in the center of the weld metal is isometric grain, whose direction coefficient X=1 In order to avoid welding crack and improve welding technology the weld heat input should be strictly controlled in 10-16 kJ/cm. Thus, the main microstructure in the weld metals is fine acicular ferrite and the content of pro eutectic ferrite is limited. The impact toughness in the weld metals of HQ130+QJ63 steels can be ensured and can meet the requirements for application in engineering and machinery.
基金Project Sponsored by Ministry of Science and Technology of China(G1998061513)
文摘With TEM、SEM, various high temperature deformed structures in W9Mo3Cr4V steel were investigated. The sub structures,recrystallized nuclei, as well as the dynamic precipitation were also studied and analyzed. The relationship between recrystallized structures and dynamic precipitation was discussed. The results showed that the deformed structures in W9Mo3Cr4V steel are more complicated than those in low alloy steels. Because W9Mo3Cr4V steel is a high speed steel, there are a large number of residual carbides on the matrix. Also, much dynamic precipitating carbides will precipitate during deformation at high temperature.
基金The project supported by PUMC Youth Fund(3332015047)Fundamental Research Funds for the Central Universities,Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine(Natural Medicine)and Translational Medicine,Institute of Medical Plant Development,Peking Union Medical College and Chinese Academy of Medical Sciencesby the National Science and Technology Major Project and Scientific Researchers Aiding Enterprise Item(2012ZX09301-002-001-026 and 2012ZX09501001-004)from the Ministry of Science and Technology of China
文摘OBJECTIVE The present study was designed to investigate anticancer effect of zeylenone(Zey)on K562 cells derived from chronic myelogenous leukemia(CML)both in vitro and in vivo,followed by exploring the underlying mechanisms.METHODS Initially,the effects of Zey on cel viability,proliferation,and apoptosis were measured in K562 cells by MTT,soft agar assay,AO/EB staining,hoechst 33258 staining and flow cytometric analysis after they were treated with Zey for indicated time,the involving signaling pathways were then investigated by JC-1,real-time quantitative polymerase chain reaction(RT-q PCR),Western blotting and immunofluorescence analysis.Furthermore,the in vivo anti-tumoractivity of Zey was assessed with nude xenografts and the involving mechanism was confirmed by immunohistochemical(IHC)and histopathological analysis.RESULTS We identified that Zey dose-dependently decreased cell viability,colony formation and expression of Proliferating Cell Nuclear Antigen(PCNA),and significantly induced K562 cell apoptosis via regulating Bcl-2 family members,decreasing mitochondrial transmembrane potential,and activating caspase-3,caspase-9,and caspase-8(P<0.05 or P<0.01).Further study revealed that Zey significantly inhibited phosphorylation of Jak2 and Src and downregulated their downstream proteins,including stat3,PI3K/AKT/m TOR,and ERK1/2 signaling pathways(P<0.05 or P<0.01).Zey also suppressed tumor growth with low toxicity in mouse xenograft model of K562cells through decreasing expression of Jak2 and Src.CONCLUSION Our data demonstrated that Zey substantially suppressed K562 cells both in vitro and in vivo through Jak2 and Src pathways.These findings suggest the potential of Zey as an effective anticancer agent in CML treatment.
文摘The application of parametric modeling method of Pro/E in the modular dougong is researched,and the disassembly and assembly operation of the dougong is implemented through the collision detection by the coordinate method in VRML. Direct interaction of VRML scene at the Web is implemented by using Java Script to manipulate VRML Object DOM.
