SWEETs （sugars will eventually be exported transporters） are a novel class of recently identified sugar transporters that play important roles in diverse physiological processes. However, only a few species of the p...SWEETs （sugars will eventually be exported transporters） are a novel class of recently identified sugar transporters that play important roles in diverse physiological processes. However, only a few species of the plant SWEETgene family have been functionally identified. Up till now, there has been no systematic analysis of the SWEETgene family in Cucurbitaceae crops. Here, a genome-wide characterization of this family was conducted in cucumber（Cucumis sativus L.）. A total of 17 CsSWEETgenes were identified, which are not evenly distributed over the seven cucumber chromosomes. Cucumber SWEET protein sequences possess seven conserved domains and two putative serine phosphorylation sites. The phylo- genetic tree of the SWEET genes in cucumber, Arabidopsis thaliana, and Oryza sativa was constructed, and all the SWEET genes were divided into four clades. In addition, a number of putative cis-elements were identified in the promoter regions of these CsSWEET genes： nine types involved in phytohormone responses and eight types involved in stress responses. Moreover, the transcript levels of CsSWEETgenes were analyzed in various tissues using quantitative real-time polymerase chain reaction. A majority （70.58%） of the CsSWEET genes were confined to reproductive tissue development. Finally, 18 putative watermelon ClaSWEETgenes and 18 melon CmSWEETgenes were identified that showed a high degree of similarity with CsSWEETgenes. The results from this study provided a basic understanding of the CsSWEETgenes and may also facilitate future research to elucidate the function of SWEET genes in cucumber and other Cucurbitaceae crops.展开更多
Objective:To observe the clinical efficacy of auricular point sticking at different points to relieve the pain in arteriovenous fistula puncture.Methods:A total of 42 patients with arteriovenous fistula were randomize...Objective:To observe the clinical efficacy of auricular point sticking at different points to relieve the pain in arteriovenous fistula puncture.Methods:A total of 42 patients with arteriovenous fistula were randomized into a Shenmen(TF4)group and an Elbow(SF3)group by the random number table method,with 21 cases in each group.After enrolled into different groups,before the dialysis,patients were given auricular point sticking with Wang Bu Liu Xing(Semen Vaccariae)seeds at Shenmen(TF4)and Elbow(SF3),respectively.Patients were asked to press the seeds themselves for 2 min each time,four times a day,and an additional 5-15 min before the arteriovenous fistula puncture.Intensive pressing was offered duri ng the pun cture,15・20 presses for each time,and the plasters were cha nged every 2-3 d.The nu merical rati ng scale(NRS)was used to score the pain level one week before and after auricular point sticking.The NRS score was then compared and analyzed.Results:The intra-group comparison showed that the changes of NRS score in both groups were statistically sigrdficant after auricular point sticking(both P<0.05).After the treatment,there was no sigrdficant differenee in NRS score between the two groups(P>0.05).Conclusion:Auricular point sticking at Shenmen(TF4)or Elbow(SF3)can effectively relieve the pain of arteriovenous fistula puncture,and these two points have equivale nt an algesic effect.展开更多
基金supported by the National Natural Science Foundation of China (31301792)the Beijing Natural Science Foundation, China (6142010)the Youth Scientific Research Funds of the Beijing Academy of Agriculture and Forestry Sciences, China (QNJJ201401)
文摘SWEETs （sugars will eventually be exported transporters） are a novel class of recently identified sugar transporters that play important roles in diverse physiological processes. However, only a few species of the plant SWEETgene family have been functionally identified. Up till now, there has been no systematic analysis of the SWEETgene family in Cucurbitaceae crops. Here, a genome-wide characterization of this family was conducted in cucumber（Cucumis sativus L.）. A total of 17 CsSWEETgenes were identified, which are not evenly distributed over the seven cucumber chromosomes. Cucumber SWEET protein sequences possess seven conserved domains and two putative serine phosphorylation sites. The phylo- genetic tree of the SWEET genes in cucumber, Arabidopsis thaliana, and Oryza sativa was constructed, and all the SWEET genes were divided into four clades. In addition, a number of putative cis-elements were identified in the promoter regions of these CsSWEET genes： nine types involved in phytohormone responses and eight types involved in stress responses. Moreover, the transcript levels of CsSWEETgenes were analyzed in various tissues using quantitative real-time polymerase chain reaction. A majority （70.58%） of the CsSWEET genes were confined to reproductive tissue development. Finally, 18 putative watermelon ClaSWEETgenes and 18 melon CmSWEETgenes were identified that showed a high degree of similarity with CsSWEETgenes. The results from this study provided a basic understanding of the CsSWEETgenes and may also facilitate future research to elucidate the function of SWEET genes in cucumber and other Cucurbitaceae crops.
文摘Objective:To observe the clinical efficacy of auricular point sticking at different points to relieve the pain in arteriovenous fistula puncture.Methods:A total of 42 patients with arteriovenous fistula were randomized into a Shenmen(TF4)group and an Elbow(SF3)group by the random number table method,with 21 cases in each group.After enrolled into different groups,before the dialysis,patients were given auricular point sticking with Wang Bu Liu Xing(Semen Vaccariae)seeds at Shenmen(TF4)and Elbow(SF3),respectively.Patients were asked to press the seeds themselves for 2 min each time,four times a day,and an additional 5-15 min before the arteriovenous fistula puncture.Intensive pressing was offered duri ng the pun cture,15・20 presses for each time,and the plasters were cha nged every 2-3 d.The nu merical rati ng scale(NRS)was used to score the pain level one week before and after auricular point sticking.The NRS score was then compared and analyzed.Results:The intra-group comparison showed that the changes of NRS score in both groups were statistically sigrdficant after auricular point sticking(both P<0.05).After the treatment,there was no sigrdficant differenee in NRS score between the two groups(P>0.05).Conclusion:Auricular point sticking at Shenmen(TF4)or Elbow(SF3)can effectively relieve the pain of arteriovenous fistula puncture,and these two points have equivale nt an algesic effect.
