The fall armyworm,Spodoptera frugiperda,which destroys many economic crops such as rice and maize,has recently invaded China.Insect viruses as biological control agents play important roles in killing pests.One potent...The fall armyworm,Spodoptera frugiperda,which destroys many economic crops such as rice and maize,has recently invaded China.Insect viruses as biological control agents play important roles in killing pests.One potential viral insecticide is the environmentally highly infective and virulent densovirus.We successfully rescued Junonia coenia densovirus(JcDV)using its infectious clone in different insect cell lines and larvae of three insect species.Results showed that the lysate of cultured insect cells transfected by the JcDV infectious clone killed the 2 nd instar S.frugiperda.The LD50 of homogenate from JcDV-infected Spodoptera litura to the 2 nd instar S.frugiperda(1.76×10^(8)viral genome copies per larva during 10 d post infection)was higher than that of the 2 nd instar S.litura(7.39×10^(7)Jc DV genome copies)or Helicoverpa armigera larvae(9.71×10^(7)JcDV genome copies).The LT50 of the S.litura homogenate(2.60×10^(9)viral genome copies each larva)to the 2 nd instar S.frugiperda was 6.96 d,longer than that of the S.litura(6.18 d)or the 2 nd instar H.armigera(5.94 d).JcDV could infect the fat body of H.armigera,but not S.frugiperda or S.litura.Although JcDV can infect all three lepidopteran species,their susceptibility to the virus differs.JcDV has great potential as a biological control agent against pests such as S.frugiperda.展开更多
目的鉴定一个富脯氨酸跨膜蛋白2(proline-rich transmembrane protein 2,PRRT2)相关发作性疾病家系的PRRT2基因突变类型,并分析其临床表型特点。方法该家系有3代7人,其中5人(先证者及其母亲、哥哥、舅舅、外婆)有抽搐史。对上述5名有抽...目的鉴定一个富脯氨酸跨膜蛋白2(proline-rich transmembrane protein 2,PRRT2)相关发作性疾病家系的PRRT2基因突变类型,并分析其临床表型特点。方法该家系有3代7人,其中5人(先证者及其母亲、哥哥、舅舅、外婆)有抽搐史。对上述5名有抽搐史的家系成员及先证者的父亲采集外周静脉血,提取全血基因组DNA,进行全外显子测序。根据全外显子测序结果筛选出的致病基因突变位点(位于PRRT2基因第2外显子),采用Sanger测序法对每个家系成员的目标位点进行验证。结果该家系5例患者中,2例符合良性家族性婴幼儿癫痫诊断,1例符合发作性运动诱发性运动障碍诊断,2例符合热性惊厥诊断。5例患者均存在PRRT2基因第2外显子c.649dup C(p.R217Pfs*8)杂合突变,先证者的父亲未发现该突变位点。结论该家系存在PRRT2基因c.649dupC杂合突变,导致PRRT2相关发作性疾病。展开更多
基金supported by the National Key R&D Program of China(2017YFD0200400)the Natural Science Foundation of Hubei Province,China(2017CFB241)。
文摘The fall armyworm,Spodoptera frugiperda,which destroys many economic crops such as rice and maize,has recently invaded China.Insect viruses as biological control agents play important roles in killing pests.One potential viral insecticide is the environmentally highly infective and virulent densovirus.We successfully rescued Junonia coenia densovirus(JcDV)using its infectious clone in different insect cell lines and larvae of three insect species.Results showed that the lysate of cultured insect cells transfected by the JcDV infectious clone killed the 2 nd instar S.frugiperda.The LD50 of homogenate from JcDV-infected Spodoptera litura to the 2 nd instar S.frugiperda(1.76×10^(8)viral genome copies per larva during 10 d post infection)was higher than that of the 2 nd instar S.litura(7.39×10^(7)Jc DV genome copies)or Helicoverpa armigera larvae(9.71×10^(7)JcDV genome copies).The LT50 of the S.litura homogenate(2.60×10^(9)viral genome copies each larva)to the 2 nd instar S.frugiperda was 6.96 d,longer than that of the S.litura(6.18 d)or the 2 nd instar H.armigera(5.94 d).JcDV could infect the fat body of H.armigera,but not S.frugiperda or S.litura.Although JcDV can infect all three lepidopteran species,their susceptibility to the virus differs.JcDV has great potential as a biological control agent against pests such as S.frugiperda.
文摘目的鉴定一个富脯氨酸跨膜蛋白2(proline-rich transmembrane protein 2,PRRT2)相关发作性疾病家系的PRRT2基因突变类型,并分析其临床表型特点。方法该家系有3代7人,其中5人(先证者及其母亲、哥哥、舅舅、外婆)有抽搐史。对上述5名有抽搐史的家系成员及先证者的父亲采集外周静脉血,提取全血基因组DNA,进行全外显子测序。根据全外显子测序结果筛选出的致病基因突变位点(位于PRRT2基因第2外显子),采用Sanger测序法对每个家系成员的目标位点进行验证。结果该家系5例患者中,2例符合良性家族性婴幼儿癫痫诊断,1例符合发作性运动诱发性运动障碍诊断,2例符合热性惊厥诊断。5例患者均存在PRRT2基因第2外显子c.649dup C(p.R217Pfs*8)杂合突变,先证者的父亲未发现该突变位点。结论该家系存在PRRT2基因c.649dupC杂合突变,导致PRRT2相关发作性疾病。
