Background and objective:Commonly plaguing in the frigid zone of the world,vitamin D deficiency,as indicated by low levels of 25-hydroxyvitamin D,exacerbated inflammatory responses and impaired endothelial function.Le...Background and objective:Commonly plaguing in the frigid zone of the world,vitamin D deficiency,as indicated by low levels of 25-hydroxyvitamin D,exacerbated inflammatory responses and impaired endothelial function.Leukoaraiosis(LA)is a prevalent cause of cognitive dysfunction in the elderly and is potentially associated with inflammatory responses.This study aimed to investigate the impact of vitamin D on the severity of LA.Methods:Patients with LA were categorized based on 3.0 T brain MRI findings into mild(N=43),moderate(N=40),or severe groups(N=29)using the Fazekas scale(scoring 1-6).A control group consisting of 41 healthy individuals was included.Serum fibrinogen C,homocysteine,plasma 25-hydroxyvitamin D,and intercellular cell adhesion molecule-1(ICAM-1)levels were measured using ELISA.Results:All LA severity groups exhibited lower plasma 25-hydroxyvitamin D levels compared to the control group,with a more pronounced decrease observed as LA severity increased.Low plasma 25-hydroxyvitamin D was identified as an independent risk factor for LA(P<0.05)according to Multiple logistic regression analysis.Additionally,a negative association was observed between 25-hydroxyvitamin D and vascular inflammatory factor ICAM-1.Conclusions:Disease severity positively correlated with levels of the inflammatory marker ICAM-1,worsening as plasma 25-hydroxyvitamin D concentration decreased.Low 25-hydroxyvitamin D emerged as an independent risk factor for LA,potentially exacerbating the inflammatory response.These findings suggest 25-hydroxyvitamin D supplementation as a potential therapeutic approach for LA.展开更多
An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were ach...An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were achieved.Hence,we reported a succinct enhanced luminescence strategy to reduce single-triplet energy split by the tunability of ILs.This strategy could be extended to more kinds of ILs by the virtue of a preliminary DFT calculation screening.Moreover,the optical feature of IL sensor made it a promising candidate as a gas fluorescence sensor.展开更多
The hexagonal to orthorhombic(HO)transformation fromβ-Ni_(3)Sn_(2)(hexagonal)phase toα'-Ni_(3)Sn_(2)(orthorhombic)phase was confirmed in directionally solidified Sn−Ni peritectic alloys.It is shown that the reme...The hexagonal to orthorhombic(HO)transformation fromβ-Ni_(3)Sn_(2)(hexagonal)phase toα'-Ni_(3)Sn_(2)(orthorhombic)phase was confirmed in directionally solidified Sn−Ni peritectic alloys.It is shown that the remelting/resolidification process which is caused by both the temperature gradient zone melting(TGZM)and Gibbs−Thomson(G−T)effects can take place on secondary dendrites.Besides,the intersection angle between the primary dendrite stem and secondary branch(θ)is found to increase fromπ/3 toπ/2 as the solidification proceeds.This is the morphological feature of the HO transformation,which can change the diffusion distance of the remelting/resolidification process.Thus,a diffusion-based analytical model is established to describe this process through the specific surface area(S_(V))of dendrites.The theoretical prediction demonstrates that the remelting/resolidification process is restricted when the HO transformation occurs during peritectic solidification.In addition,the slope of the prediction curves is changed,indicating the variation of the local remelting/resolidification rates.展开更多
Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQ...Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQS,ionic liquid[P66614][HQS]emitted a certain amount of fluorescence,which could be attributed to the well-delocalized frontier orbitals and its charge transfer character,as demonstrated by quantum chemical calculation.Considering the interaction of[P66614][HQS]with metal ions,the application for detecting specific substance as a chemical sensor,such as Al3+was investigated.Compared with the traditional probe HQS,significant improvements in Al^3+detecting was achieved by[P66614][HQS]with stronger binding ability,better sensitivity and selectivity.The better performance of[P66614][HQS]was contributed to the changed charge distribution,leading to the stronger binding interaction.We believe that this new fluorescent ionic liquid exhibited unique properties in detecting Al^3+in aqueous solution,which would broaden the application of ionic liquids.展开更多
1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins...1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins,abscisic acid,and chlorophyll.The objective of this study was to determine the functional role of PpDXS1 in plant growth and development in Kentucky bluegrass(Poa pratensis L.).The PpDXS1 gene has a 2139 bp open reading frame that encodes a polypeptide of 712 amino acids with a calculated molecular weight of 76.7 kDa.PpDXS1 gene expression was the highest in leaves.Moreover,the PpDXS1 promoter contained several hormone response elements and gene expression was induced by exogenous treatment with gibberellin,abscisic acid,jasmonate,and pathogen infection.Functional analysis indicated that underexpression of PpDXS1 gene in Poa pratensis decreased plant height and endogenous gibberellin and indole acetic acid production,but promoted abscisic acid accumulation.Furthermore,transcriptome analysis and qRT-PCR results showed that the expression levels of related genes involved in the phytohormone biosynthesis and signal transduction were differentially regulated by PpDXS1 in transgenic Poa pratensis.Overall,these results indicated that PpDXS1 has strong effects on plant height and accumulation of phytohormones,and provided a preliminary understanding of molecular characterization,expression and function of PpDXS1 in Poa pratensis.展开更多
To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)pre...To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)prediction model based on the incremental attention mechanism.Firstly,a traversal search is conducted through the traversal layer for finite parameters in the phase space.Then,an incremental attention layer is utilized for parameter judgment based on the dimension weight criteria(DWC).The phase space parameters that best meet DWC are selected and fed into the input layer.Finally,the constructed CNN-LSTM network extracts spatio-temporal features and provides the final prediction results.The model is verified using Logistic,Lorenz,and sunspot chaotic time series,and the performance is compared from the two dimensions of prediction accuracy and network phase space structure.Additionally,the CNN-LSTM network based on incremental attention is compared with long short-term memory(LSTM),convolutional neural network(CNN),recurrent neural network(RNN),and support vector regression(SVR)for prediction accuracy.The experiment results indicate that the proposed composite network model possesses enhanced capability in extracting temporal features and achieves higher prediction accuracy.Also,the algorithm to estimate the phase space parameter is compared with the traditional CAO,false nearest neighbor,and C-C,three typical methods for determining the chaotic phase space parameters.The experiments reveal that the phase space parameter estimation algorithm based on the incremental attention mechanism is superior in prediction accuracy compared with the traditional phase space reconstruction method in five networks,including CNN-LSTM,LSTM,CNN,RNN,and SVR.展开更多
The integration of electricity technology and information technology,such as the Internet of Things(IoT),enables the construction of new power systems,along with the inno-vation of application scenarios and business s...The integration of electricity technology and information technology,such as the Internet of Things(IoT),enables the construction of new power systems,along with the inno-vation of application scenarios and business scope.The key technologies of power IoT and the data flow process are summarised first.The IoT technology and application scenario requirements of power generation,transmission,loading,and storage of new power systems are studied.Thus,the nature of the collaborative development of the digital power grid and the IoT is demonstrated from the perspective of data processing in power IoT and application requirements in power systems.The key problems and so-lutions faced by the power IoT under the digital transformation are described,and the cross-integration of key technologies and promotion of application scenario innovation are prospected.Finally,the key issues of future technological development were dis-cussed,providing reference ideas for fully leveraging the value of energy and electricity data production factors and promoting the construction of a digital electricity ecosystem.展开更多
通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中...通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中心的临床教学法能充分发挥本科生的主现能动性,是提高本科生理论知识和实践操作等综合素质的有效途径。在传统授课中加入病例讨论教学,能使学生更好地掌握耳鼻咽喉头颈外科学的知识。在传统教学模式上的创新,可改善传统教学模式中枯燥、平述式教学模式,创新教学模式下有助于整体耳鼻喉科临床教学水平的提高。展开更多
The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues,...The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues, glial fibrillary acidic protein and neurofilament protein changes can reflect the condition of injured neurons and astrocytes, while vascular endothelial growth factor and basic fibroblast growth factor changes can indicate angiogenesis. In the present study, we induced ischemic brain injury in the rhesus macaque by electrocoagulation of the M1 segment of the right middle cerebral artery. The motor relearning program was conducted for 60 days from the third day after model establishment. Immunohistochemistry and single-photon emission CT showed that the numbers of glial fibrillary acidic protein-, neurofilament protein-, vascular endothelial growth factor- and basic fibroblast growth factor-positive cells were significantly increased in the infarcted side compared with the contralateral hemisphere following the motor relearning program. Moreover, cerebral blood flow in the infarcted side was significantly improved. The clinical rating scale for stroke was used to assess neurological function changes in the rhesus macaque following the motor relearning program. Results showed that motor function was improved, and problems with consciousness, self-care ability and balance function were significantly ameliorated. These findings indicate that the motor relearning program significantly promoted neuronal regeneration, repair and angiogenesis in the surroundings of the infarcted hemisphere, and improve neurological function in the rhesus macaque following brain ischemia.展开更多
Excitation of metal-free graphitic carbon nitride(g-C3N4) under visible light can successfully achieve efficient activation of peroxymonosulfate(PMS). Synergistic effects and involved mechanism were systematically inv...Excitation of metal-free graphitic carbon nitride(g-C3N4) under visible light can successfully achieve efficient activation of peroxymonosulfate(PMS). Synergistic effects and involved mechanism were systematically investigated using a light-inert endocrine disrupting compound, dimethyl phthalate(DMP), as the target pollutant. Under visible light irradiation, DMP could not be degraded by direct g-C3 N4-mediated photocatalysis, while in the presence of PMS, the dominant radicals were converted from ·O2 to SO4·– and ·OH, resulting in effective DMP degradation and mineralization. Results showed that higher dosage of PMS or g-C3 N4 could increase the activation amount of PMS and corresponding DMP degradation efficiency, but the latter approach was more productive in terms of making the most of PMS. High DMP concentration hindered effective contact between PMS and g-C3 N4, but could provide efficient use of PMS. Higher DMP degradation efficiency was achieved at p H lower than the point of zero charge(5.4). Based on intermediates identification, the DMP degradation was found mainly through radical attack(·OH and SO4·–) of the benzene ring and oxidation of the aliphatic chains.展开更多
Regenerative endodontics(RE)therapy means physiologically replacing damaged pulp tissue and regaining functional dentin–pulp complex.Current clinical RE procedures recruit endogenous stem cells from the apical papill...Regenerative endodontics(RE)therapy means physiologically replacing damaged pulp tissue and regaining functional dentin–pulp complex.Current clinical RE procedures recruit endogenous stem cells from the apical papilla,periodontal tissue,bone marrow and peripheral blood,with or without application of scaffolds and growth factors in the root canal space,resulting in cementum-like and bone-like tissue formation.Without the involvement of dental pulp stem cells(DPSCs),it is unlikely that functional pulp regeneration can be achieved,even though acceptable repair can be acquired.DPSCs,due to their specific odontogenic potential,high proliferation,neurovascular property,and easy accessibility,are considered as the most eligible cell source for dentin–pulp regeneration.The regenerative potential of DPSCs has been demonstrated by recent clinical progress.DPSC transplantation following pulpectomy has successfully reconstructed neurovascularized pulp that simulates the physiological structure of natural pulp.The self-renewal,proliferation,and odontogenic differentiation of DPSCs are under the control of a cascade of transcription factors.Over recent decades,epigenetic modulations implicating histone modifications,DNA methylation,and noncoding(nc)RNAs have manifested as a new layer of gene regulation.These modulations exhibit a profound effect on the cellular activities of DPSCs.In this review,we offer an overview about epigenetic regulation of the fate of DPSCs;in particular,on the proliferation,odontogenic differentiation,angiogenesis,and neurogenesis.We emphasize recent discoveries of epigenetic molecules that can alter DPSC status and promote pulp regeneration through manipulation over epigenetic profiles.展开更多
Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),l...Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.展开更多
基金This work was supported by the National Natural Science Foundation of Heilongjiang Province of China(No.LH2020H051)Key R&D projects of Natural Science Foundation of Heilongjiang Province(No.2023ZX06C03)Foundation of Harbin Science Technology Bureau of China(No.2014RFQGJ042).
文摘Background and objective:Commonly plaguing in the frigid zone of the world,vitamin D deficiency,as indicated by low levels of 25-hydroxyvitamin D,exacerbated inflammatory responses and impaired endothelial function.Leukoaraiosis(LA)is a prevalent cause of cognitive dysfunction in the elderly and is potentially associated with inflammatory responses.This study aimed to investigate the impact of vitamin D on the severity of LA.Methods:Patients with LA were categorized based on 3.0 T brain MRI findings into mild(N=43),moderate(N=40),or severe groups(N=29)using the Fazekas scale(scoring 1-6).A control group consisting of 41 healthy individuals was included.Serum fibrinogen C,homocysteine,plasma 25-hydroxyvitamin D,and intercellular cell adhesion molecule-1(ICAM-1)levels were measured using ELISA.Results:All LA severity groups exhibited lower plasma 25-hydroxyvitamin D levels compared to the control group,with a more pronounced decrease observed as LA severity increased.Low plasma 25-hydroxyvitamin D was identified as an independent risk factor for LA(P<0.05)according to Multiple logistic regression analysis.Additionally,a negative association was observed between 25-hydroxyvitamin D and vascular inflammatory factor ICAM-1.Conclusions:Disease severity positively correlated with levels of the inflammatory marker ICAM-1,worsening as plasma 25-hydroxyvitamin D concentration decreased.Low 25-hydroxyvitamin D emerged as an independent risk factor for LA,potentially exacerbating the inflammatory response.These findings suggest 25-hydroxyvitamin D supplementation as a potential therapeutic approach for LA.
基金the support of the Natural Science Foundation of China(21776239)Zhejiang Provincial Natural Science Foundation of China(LZ17B060001)+1 种基金the Fundamental Research Funds of the Central UniversitiesResearch Initiation Fund of Zhejiang University of Technology(2020101005929)。
文摘An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were achieved.Hence,we reported a succinct enhanced luminescence strategy to reduce single-triplet energy split by the tunability of ILs.This strategy could be extended to more kinds of ILs by the virtue of a preliminary DFT calculation screening.Moreover,the optical feature of IL sensor made it a promising candidate as a gas fluorescence sensor.
基金the support from the Natural Science Foundation of China(No.51871118)Open Project of Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education,Lanzhou University,China(No.LZUMMM2021005)+1 种基金the Science and Technology Project of Lanzhou City,China(No.2019-1-30)State Key Laboratory of Special Rare Metal Materials,China(No.SKL2020K003)。
文摘The hexagonal to orthorhombic(HO)transformation fromβ-Ni_(3)Sn_(2)(hexagonal)phase toα'-Ni_(3)Sn_(2)(orthorhombic)phase was confirmed in directionally solidified Sn−Ni peritectic alloys.It is shown that the remelting/resolidification process which is caused by both the temperature gradient zone melting(TGZM)and Gibbs−Thomson(G−T)effects can take place on secondary dendrites.Besides,the intersection angle between the primary dendrite stem and secondary branch(θ)is found to increase fromπ/3 toπ/2 as the solidification proceeds.This is the morphological feature of the HO transformation,which can change the diffusion distance of the remelting/resolidification process.Thus,a diffusion-based analytical model is established to describe this process through the specific surface area(S_(V))of dendrites.The theoretical prediction demonstrates that the remelting/resolidification process is restricted when the HO transformation occurs during peritectic solidification.In addition,the slope of the prediction curves is changed,indicating the variation of the local remelting/resolidification rates.
基金We acknowledge the support of the National Key Basic Research Program of China(2015CB251401)the Natural Science Foundation of China(21776239)+1 种基金Zhejiang Provincial Natural Science Foundation of China(LZ17B060001)the Fundamental Research Funds of the Central Universities,the instrument support of professor Peng Xiaogang,professor Jin Yizheng and associate professor Qin Haiyan.
文摘Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQS,ionic liquid[P66614][HQS]emitted a certain amount of fluorescence,which could be attributed to the well-delocalized frontier orbitals and its charge transfer character,as demonstrated by quantum chemical calculation.Considering the interaction of[P66614][HQS]with metal ions,the application for detecting specific substance as a chemical sensor,such as Al3+was investigated.Compared with the traditional probe HQS,significant improvements in Al^3+detecting was achieved by[P66614][HQS]with stronger binding ability,better sensitivity and selectivity.The better performance of[P66614][HQS]was contributed to the changed charge distribution,leading to the stronger binding interaction.We believe that this new fluorescent ionic liquid exhibited unique properties in detecting Al^3+in aqueous solution,which would broaden the application of ionic liquids.
基金supported by the National Natural Science Foundation of China(No.31302016).
文摘1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins,abscisic acid,and chlorophyll.The objective of this study was to determine the functional role of PpDXS1 in plant growth and development in Kentucky bluegrass(Poa pratensis L.).The PpDXS1 gene has a 2139 bp open reading frame that encodes a polypeptide of 712 amino acids with a calculated molecular weight of 76.7 kDa.PpDXS1 gene expression was the highest in leaves.Moreover,the PpDXS1 promoter contained several hormone response elements and gene expression was induced by exogenous treatment with gibberellin,abscisic acid,jasmonate,and pathogen infection.Functional analysis indicated that underexpression of PpDXS1 gene in Poa pratensis decreased plant height and endogenous gibberellin and indole acetic acid production,but promoted abscisic acid accumulation.Furthermore,transcriptome analysis and qRT-PCR results showed that the expression levels of related genes involved in the phytohormone biosynthesis and signal transduction were differentially regulated by PpDXS1 in transgenic Poa pratensis.Overall,these results indicated that PpDXS1 has strong effects on plant height and accumulation of phytohormones,and provided a preliminary understanding of molecular characterization,expression and function of PpDXS1 in Poa pratensis.
文摘To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)prediction model based on the incremental attention mechanism.Firstly,a traversal search is conducted through the traversal layer for finite parameters in the phase space.Then,an incremental attention layer is utilized for parameter judgment based on the dimension weight criteria(DWC).The phase space parameters that best meet DWC are selected and fed into the input layer.Finally,the constructed CNN-LSTM network extracts spatio-temporal features and provides the final prediction results.The model is verified using Logistic,Lorenz,and sunspot chaotic time series,and the performance is compared from the two dimensions of prediction accuracy and network phase space structure.Additionally,the CNN-LSTM network based on incremental attention is compared with long short-term memory(LSTM),convolutional neural network(CNN),recurrent neural network(RNN),and support vector regression(SVR)for prediction accuracy.The experiment results indicate that the proposed composite network model possesses enhanced capability in extracting temporal features and achieves higher prediction accuracy.Also,the algorithm to estimate the phase space parameter is compared with the traditional CAO,false nearest neighbor,and C-C,three typical methods for determining the chaotic phase space parameters.The experiments reveal that the phase space parameter estimation algorithm based on the incremental attention mechanism is superior in prediction accuracy compared with the traditional phase space reconstruction method in five networks,including CNN-LSTM,LSTM,CNN,RNN,and SVR.
基金National Natural Science Foundation of China,Grant/Award Number:52177085DGRI‐CSG Innovative Project,Grant/Award Number:210000KK52220036。
文摘The integration of electricity technology and information technology,such as the Internet of Things(IoT),enables the construction of new power systems,along with the inno-vation of application scenarios and business scope.The key technologies of power IoT and the data flow process are summarised first.The IoT technology and application scenario requirements of power generation,transmission,loading,and storage of new power systems are studied.Thus,the nature of the collaborative development of the digital power grid and the IoT is demonstrated from the perspective of data processing in power IoT and application requirements in power systems.The key problems and so-lutions faced by the power IoT under the digital transformation are described,and the cross-integration of key technologies and promotion of application scenario innovation are prospected.Finally,the key issues of future technological development were dis-cussed,providing reference ideas for fully leveraging the value of energy and electricity data production factors and promoting the construction of a digital electricity ecosystem.
文摘通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中心的临床教学法能充分发挥本科生的主现能动性,是提高本科生理论知识和实践操作等综合素质的有效途径。在传统授课中加入病例讨论教学,能使学生更好地掌握耳鼻咽喉头颈外科学的知识。在传统教学模式上的创新,可改善传统教学模式中枯燥、平述式教学模式,创新教学模式下有助于整体耳鼻喉科临床教学水平的提高。
基金supported by the Combined pecific Foundation of Department of Science and Technology of Yunnan Province and Kunming Medical University,No.2008CD037
文摘The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues, glial fibrillary acidic protein and neurofilament protein changes can reflect the condition of injured neurons and astrocytes, while vascular endothelial growth factor and basic fibroblast growth factor changes can indicate angiogenesis. In the present study, we induced ischemic brain injury in the rhesus macaque by electrocoagulation of the M1 segment of the right middle cerebral artery. The motor relearning program was conducted for 60 days from the third day after model establishment. Immunohistochemistry and single-photon emission CT showed that the numbers of glial fibrillary acidic protein-, neurofilament protein-, vascular endothelial growth factor- and basic fibroblast growth factor-positive cells were significantly increased in the infarcted side compared with the contralateral hemisphere following the motor relearning program. Moreover, cerebral blood flow in the infarcted side was significantly improved. The clinical rating scale for stroke was used to assess neurological function changes in the rhesus macaque following the motor relearning program. Results showed that motor function was improved, and problems with consciousness, self-care ability and balance function were significantly ameliorated. These findings indicate that the motor relearning program significantly promoted neuronal regeneration, repair and angiogenesis in the surroundings of the infarcted hemisphere, and improve neurological function in the rhesus macaque following brain ischemia.
基金supported by the Natural Science Foundation of Jiangsu Province(BK20160936,BK20160938)the National Natural Science Foundation of China(51708297)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)~~
文摘Excitation of metal-free graphitic carbon nitride(g-C3N4) under visible light can successfully achieve efficient activation of peroxymonosulfate(PMS). Synergistic effects and involved mechanism were systematically investigated using a light-inert endocrine disrupting compound, dimethyl phthalate(DMP), as the target pollutant. Under visible light irradiation, DMP could not be degraded by direct g-C3 N4-mediated photocatalysis, while in the presence of PMS, the dominant radicals were converted from ·O2 to SO4·– and ·OH, resulting in effective DMP degradation and mineralization. Results showed that higher dosage of PMS or g-C3 N4 could increase the activation amount of PMS and corresponding DMP degradation efficiency, but the latter approach was more productive in terms of making the most of PMS. High DMP concentration hindered effective contact between PMS and g-C3 N4, but could provide efficient use of PMS. Higher DMP degradation efficiency was achieved at p H lower than the point of zero charge(5.4). Based on intermediates identification, the DMP degradation was found mainly through radical attack(·OH and SO4·–) of the benzene ring and oxidation of the aliphatic chains.
基金Supported by National Natural Science Foundation of China,No.81800929 and No.81771033Sichuan Science and Technology Program,No.2019JDRC0096and Research and Develop Program,West China Hospital of Stomatology Sichuan University,No.LCYJ2019-24.
文摘Regenerative endodontics(RE)therapy means physiologically replacing damaged pulp tissue and regaining functional dentin–pulp complex.Current clinical RE procedures recruit endogenous stem cells from the apical papilla,periodontal tissue,bone marrow and peripheral blood,with or without application of scaffolds and growth factors in the root canal space,resulting in cementum-like and bone-like tissue formation.Without the involvement of dental pulp stem cells(DPSCs),it is unlikely that functional pulp regeneration can be achieved,even though acceptable repair can be acquired.DPSCs,due to their specific odontogenic potential,high proliferation,neurovascular property,and easy accessibility,are considered as the most eligible cell source for dentin–pulp regeneration.The regenerative potential of DPSCs has been demonstrated by recent clinical progress.DPSC transplantation following pulpectomy has successfully reconstructed neurovascularized pulp that simulates the physiological structure of natural pulp.The self-renewal,proliferation,and odontogenic differentiation of DPSCs are under the control of a cascade of transcription factors.Over recent decades,epigenetic modulations implicating histone modifications,DNA methylation,and noncoding(nc)RNAs have manifested as a new layer of gene regulation.These modulations exhibit a profound effect on the cellular activities of DPSCs.In this review,we offer an overview about epigenetic regulation of the fate of DPSCs;in particular,on the proliferation,odontogenic differentiation,angiogenesis,and neurogenesis.We emphasize recent discoveries of epigenetic molecules that can alter DPSC status and promote pulp regeneration through manipulation over epigenetic profiles.
基金the support by the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01006)the National Natural Science Foundation of China(NSFC:12205252)+3 种基金A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department(Y202250795)the Research Funds of Institute of Zhejiang University-Quzhou,the Basic Public Welfare Research Special Project of Zhejiang Province(LZY22B040001)the Science and Technology Project of Quzhou Research Institute,Zhejiang University(IZQ2021KJ2032)the Independent Scientific Research Project of Quzhou Research Institute,Zhejiang University(IZQ2021RCZX007)。
文摘Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.