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ATFF: Advanced Transformer with Multiscale Contextual Fusion for Medical Image Segmentation
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作者 Xinping Guo lei wang +2 位作者 Zizhen Huang Yukun Zhang Yaolong Han 《Journal of Computer and Communications》 2024年第3期238-251,共14页
Deep convolutional neural network (CNN) greatly promotes the automatic segmentation of medical images. However, due to the inherent properties of convolution operations, CNN usually cannot establish long-distance inte... Deep convolutional neural network (CNN) greatly promotes the automatic segmentation of medical images. However, due to the inherent properties of convolution operations, CNN usually cannot establish long-distance interdependence, which limits the segmentation performance. Transformer has been successfully applied to various computer vision, using self-attention mechanism to simulate long-distance interaction, so as to capture global information. However, self-attention lacks spatial location and high-performance computing. In order to solve the above problems, we develop a new medical transformer, which has a multi-scale context fusion function and can be used for medical image segmentation. The proposed model combines convolution operation and attention mechanism to form a u-shaped framework, which can capture both local and global information. First, the traditional converter module is improved to an advanced converter module, which uses post-layer normalization to obtain mild activation values, and uses scaled cosine attention with a moving window to obtain accurate spatial information. Secondly, we also introduce a deep supervision strategy to guide the model to fuse multi-scale feature information. It further enables the proposed model to effectively propagate feature information across layers, Thanks to this, it can achieve better segmentation performance while being more robust and efficient. The proposed model is evaluated on multiple medical image segmentation datasets. Experimental results demonstrate that the proposed model achieves better performance on a challenging dataset (ETIS) compared to existing methods that rely only on convolutional neural networks, transformers, or a combination of both. The mDice and mIou indicators increased by 2.74% and 3.3% respectively. 展开更多
关键词 Medical Image Segmentation Advanced Transformer Deep Supervision Attention Mechanism
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Immune regulation of the gut-brain axis and lung-brain axis involved in ischemic stroke 被引量:4
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作者 Xiaodi Xie lei wang +2 位作者 Shanshan Dong ShanChun Ge Ting Zhu 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期519-528,共10页
Local ischemia often causes a series of inflammatory reactions when both brain immune cells and the peripheral immune response are activated.In the human body,the gut and lung are regarded as the key reactional target... Local ischemia often causes a series of inflammatory reactions when both brain immune cells and the peripheral immune response are activated.In the human body,the gut and lung are regarded as the key reactional targets that are initiated by brain ischemic attacks.Mucosal microorganisms play an important role in immune regulation and metabolism and affect blood-brain barrier permeability.In addition to the relationship between peripheral organs and central areas and the intestine and lung also interact among each other.Here,we review the molecular and cellular immune mechanisms involved in the pathways of inflammation across the gut-brain axis and lung-brain axis.We found that abnormal intestinal flora,the intestinal microenvironment,lung infection,chronic diseases,and mechanical ventilation can worsen the outcome of ischemic stroke.This review also introduces the influence of the brain on the gut and lungs after stroke,highlighting the bidirectional feedback effect among the gut,lungs,and brain. 展开更多
关键词 enteric glia cells gut microbiota gut-brain axis immune response inflammation ischemic stroke lung-brain axis microglia
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Intelligent Small Sample Defect Detection of Concrete Surface Using Novel Deep Learning Integrating Improved YOLOv5 被引量:2
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作者 Yongming Han lei wang +1 位作者 Youqing wang Zhiqiang Geng 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第2期545-547,共3页
Dear Editor,This letter presents an intelligent small sample defect detection of concrete surface using novel deep learning integrating the improved YOLOv5 based on the Wasserstein GAN(WGAN)enhancement algorithm.The p... Dear Editor,This letter presents an intelligent small sample defect detection of concrete surface using novel deep learning integrating the improved YOLOv5 based on the Wasserstein GAN(WGAN)enhancement algorithm.The proposed method is capable of producing top-notch data sets to address the issues of insufficient samples and substandard quality. 展开更多
关键词 CONCRETE integrating ALGORITHM
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Alterations in the gut microbiome after transjugular intrahepatic portosystemic shunt in patients with hepatitis B virus-related portal hypertension 被引量:1
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作者 Hong-Wei Zhao Jin-Long Zhang +5 位作者 Fu-Quan Liu Zhen-Dong Yue lei wang Yu Zhang Cheng-Bin Dong Zhen-Chang wang 《World Journal of Gastroenterology》 SCIE CAS 2024年第31期3668-3679,共12页
BACKGROUND Gut microbiota(GM)affects the progression and response to treatment in liver diseases.The GM composition is diverse and associated with different etiologies of liver diseases.Notably,alterations in GM alter... BACKGROUND Gut microbiota(GM)affects the progression and response to treatment in liver diseases.The GM composition is diverse and associated with different etiologies of liver diseases.Notably,alterations in GM alterations are observed in patients with portal hypertension(PH)secondary to cirrhosis,with hepatitis B virus(HBV)infection being a major cause of cirrhosis in China.Thus,understanding the role of GM alterations in patients with HBV infection-related PH is essential.AIM To evaluate GM alterations in patients with HBV-related PH after transjugular intrahepatic portosystemic shunt(TIPS)placement.METHODS This was a prospective,observational clinical study.There were 30 patients(with a 100%technical success rate)recruited in the present study.Patients with esophagogastric variceal bleeding due to HBV infection-associated PH who underwent TIPS were enrolled.Stool samples were obtained before and one month after TIPS treatment,and GM was analyzed using 16S ribosomal RNA amplicon sequencing.RESULTS One month after TIPS placement,8 patients developed hepatic encephalopathy(HE)and were assigned to the HE group;the other 22 patients were assigned to the non-HE group.There was no substantial disparity in the abundance of GM at the phylum level between the two groups,regardless of TIPS treatment(all,P>0.05).However,following TIPS placement,the following results were observed:(1)The abundance of Haemophilus and Eggerthella increased,whereas that of Anaerostipes,Dialister,Butyricicoccus,and Oscillospira declined in the HE group;(2)The richness of Eggerthella,Streptococcus,and Bilophila increased,whereas that of Roseburia and Ruminococcus decreased in the non-HE group;and(3)Members from the pathogenic genus Morganella appeared in the HE group but not in the non-HE group.CONCLUSION Intestinal microbiota-related synergism may predict the risk of HE following TIPS placement in patients with HBVrelated PH.Prophylactic microbiome therapies may be useful for preventing and treating HE after TIPS placement. 展开更多
关键词 Transjugular intrahepatic portosystemic shunt Hepatic encephalopathy Gut microbiota Hepatitis B virus Portal hypertension
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复杂任务情境中技术如何促进部门协同——基于S市市域社会治理现代化指挥中心的案例研究 被引量:1
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作者 王磊 王小芳 《公共行政评论》 CSSCI 北大核心 2024年第1期158-177,M0006,M0007,共22页
已有研究多将技术促进部门协同理解为技术与组织互动视角下,基层政府为应对复杂任务情境采取的一种策略性行为,但对其背后的权力维度关注甚少。论文围绕权力视角下技术如何实现政府部门协同这一核心问题,选取S市市域社会治理现代化指挥... 已有研究多将技术促进部门协同理解为技术与组织互动视角下,基层政府为应对复杂任务情境采取的一种策略性行为,但对其背后的权力维度关注甚少。论文围绕权力视角下技术如何实现政府部门协同这一核心问题,选取S市市域社会治理现代化指挥中心为案例,探讨技术是否影响,以及如何影响政府部门间的权力关系。研究认为,从案例中析出的分布式治理遵从了“任务情境-技术应用-治理行为”相互适配的行动逻辑,对标复杂性任务情境,通过平台技术的协调功能,能够推动政府部门围绕“高效处置一件事”组建任务共同体。这种任务共同体暗含了以“科层为体、技术为用”的嵌套性组织结构和松散耦合性部门关系互为表里的新型权力结构——分布式权力网络。分布式治理作为根植于中国城市基层治理场景的微观理论,一方面有助于丰富基层治理机制的理论图谱,对传统基层治理机制选择的理论预设以及技术治理与科层治理共生理论进行了部分修正和改进;另一方面,通过反思技术与科层组织关系的传统研究路径,将权力维度重新拉入技术与组织关系研究,在一定程度上扩展了既有研究视域。同时,分布式治理的提出也为回答“优化基层治理数字化转型和技术化创新”之问提供了一个探索性答案。 展开更多
关键词 分布式治理 分布式权力网络 横向协同 复杂任务情境 技术治理
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丁二酸酐改性聚乙烯醇微球对热塑性淀粉性能的影响
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作者 郑瑞杭 王磊 +1 位作者 郭斌 李盘欣 《高分子材料科学与工程》 EI CAS CSCD 北大核心 2024年第9期42-49,共8页
为了提高热塑性淀粉(TPS)塑料的力学和耐水等性能,扩大其应用领域。文中以丁二酸酐(SAA)改性的聚乙烯醇微球(PVAMS)为增强体,与玉米淀粉和甘油混合制备复合材料,研究了不同SAA添加量改性的PVAMS对TPS力学性能、热性能、耐水和加工性能... 为了提高热塑性淀粉(TPS)塑料的力学和耐水等性能,扩大其应用领域。文中以丁二酸酐(SAA)改性的聚乙烯醇微球(PVAMS)为增强体,与玉米淀粉和甘油混合制备复合材料,研究了不同SAA添加量改性的PVAMS对TPS力学性能、热性能、耐水和加工性能的影响。结果表明,SAA质量分数为15%时,SAA-PVAMS/TPS的拉伸强度和断裂伸长率分别为5.01 MPa和66.7%,冲击强度为35.39 kJ/m^(2);转变温度和热稳定性略有提高;吸水率降低,表面疏水性提高,接触角最大为61.2°;平衡和峰值扭矩分别为10.51 N·m和26.35 N·m,相对比较适中,易于加工成型。因此,以SAA改性的PVAMS来增强TPS,能实现复合材料性能的有效提升。 展开更多
关键词 热塑性淀粉 聚乙烯醇微球 丁二酸酐 改性
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Application of geophysical methods in fine detection of urban concealed karst:A case study of Wuhan City,China
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作者 Dao-han Liu lei wang +3 位作者 lei Liu Jun-jie Xu Jian-qiang Wu Pan Liu 《China Geology》 CAS CSCD 2024年第3期517-532,共16页
The construction of modern livable cities faces challenges in karst areas,including ground collapse and engineering problems.Wuhan,with a population of 13.74×10^(6) and approximately 1161 km^(2)of soluble rocks i... The construction of modern livable cities faces challenges in karst areas,including ground collapse and engineering problems.Wuhan,with a population of 13.74×10^(6) and approximately 1161 km^(2)of soluble rocks in the urban area of 8569.15 km^(2),predominantly consists of concealed karst areas where occasional ground collapse events occur,posing significant threats to underground engineering projects.To address these challenges,a comprehensive geological survey was conducted in Wuhan,focusing on major karstrelated issues.Geophysical methods offer advantages over drilling in detecting concealed karst areas due to their efficiency,non-destructiveness,and flexibility.This paper reviewed the karst geological characteristics in Wuhan and the geophysical exploration methods for karst,selected eight effective geophysical methods for field experimentation,evaluated their suitability,and proposed method combinations for different karst scenarios.The results show that different geophysical methods have varying applicability for karst detection in Wuhan,and combining multiple methods enhances detection effectiveness.The specific recommendations for method combinations provided in this study serve as a valuable reference for karst detection in Wuhan. 展开更多
关键词 Ground Penetrating Radar(GPR) Electric Resistivity Tomography(ERT) Opposing-coils Transient Electromagnetic Method(OCTEM) Microtremor Array Measurements(MAM) Multi-channel Analysis of surface wave(MASW) Multi-source surface wave exploration(MSSW) Electromagnetic wave CT(EM CT) Surface Nuclear Magnetic Resonance(SNMR) Concealed karst Urban geological survey engineering
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Experimental study of the effects of a multistage pore-throat structure on the seepage characteristics of sandstones in the Beibuwan Basin:Insights into the flooding mode
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作者 lei wang Xiao lei +7 位作者 Qiao-Liang Zhang Guang-Qing Yao Bo Sui Xiao-Jun Chen Ming-Wei wang Zhen-Yu Zhou Pan-Rong wang Xiao-Dong Peng 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期1044-1061,共18页
To investigate the relationship between grain sizes, seepage capacity, and oil-displacement efficiency in the Liushagang Formation of the Beibuwan Basin, this study identifies the multistage pore-throat structure as a... To investigate the relationship between grain sizes, seepage capacity, and oil-displacement efficiency in the Liushagang Formation of the Beibuwan Basin, this study identifies the multistage pore-throat structure as a crucial factor through a comparison of oil displacement in microscopic pore-throat experiments. The two-phase flow evaluation method based on the Li-Horne model is utilized to effectively characterize and quantify the seepage characteristics of different reservoirs, closely relating them to the distribution of microscopic pores and throats. It is observed that conglomerate sandstones at different stages exhibit significant heterogeneity and noticeable differences in seepage capacity, highlighting the crucial role played by certain large pore throats in determining seepage capacity and oil displacement efficiency. Furthermore, it was found that the displacement effects of conglomeratic sandstones with strong heterogeneity were inferior to those of conventional homogeneous sandstone, as evidenced by multiple displacement experiments conducted on core samples with varying granularities and flooding systems. Subsequently, core-based experiments on associated gas flooding after water flooding were conducted to address the challenge of achieving satisfactory results in a single displacement mode for reservoirs with significant heterogeneity. The results indicate that the oil recovery rates for associated gas flooding after water flooding increased by 7.3%-16.4% compared with water flooding alone at a gas-oil ratio of approximately 7000 m^(3)/m^(3). Therefore, considering the advantages of gas flooding in terms of seepage capacity, oil exchange ratio, and the potential for two-phase production, gas flooding is recommended as an energy supplement mode for homogeneous reservoirs in the presence of sufficient gas source and appropriate tectonic angle. On the other hand, associated gas flooding after water flooding is suggested to achieve a more favorable development effect compared to a single mode of energy supplementation for strongly heterogeneous sandstone reservoirs. 展开更多
关键词 Beibuwan Basin Multistage pore-throat structure Multistage seepage characteristics Microscopic visualization HETEROGENEITY Gas/water flooding Flooding mode
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DPAL-BERT:A Faster and Lighter Question Answering Model
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作者 Lirong Yin lei wang +8 位作者 Zhuohang Cai Siyu Lu Ruiyang wang Ahmed AlSanad Salman A.AlQahtani Xiaobing Chen Zhengtong Yin Xiaolu Li Wenfeng Zheng 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第10期771-786,共16页
Recent advancements in natural language processing have given rise to numerous pre-training language models in question-answering systems.However,with the constant evolution of algorithms,data,and computing power,the ... Recent advancements in natural language processing have given rise to numerous pre-training language models in question-answering systems.However,with the constant evolution of algorithms,data,and computing power,the increasing size and complexity of these models have led to increased training costs and reduced efficiency.This study aims to minimize the inference time of such models while maintaining computational performance.It also proposes a novel Distillation model for PAL-BERT(DPAL-BERT),specifically,employs knowledge distillation,using the PAL-BERT model as the teacher model to train two student models:DPAL-BERT-Bi and DPAL-BERTC.This research enhances the dataset through techniques such as masking,replacement,and n-gram sampling to optimize knowledge transfer.The experimental results showed that the distilled models greatly outperform models trained from scratch.In addition,although the distilled models exhibit a slight decrease in performance compared to PAL-BERT,they significantly reduce inference time to just 0.25%of the original.This demonstrates the effectiveness of the proposed approach in balancing model performance and efficiency. 展开更多
关键词 DPAL-BERT question answering systems knowledge distillation model compression BERT Bi-directional long short-term memory(BiLSTM) knowledge information transfer PAL-BERT training efficiency natural language processing
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The interaction between temperature and precipitation on the potential distribution range of Betula ermanii in the alpine treeline ecotone on the Changbai Mountain
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作者 Yu Cong Yongfeng Gu +8 位作者 Wen J.wang lei wang Zhenshan Xue Yingyi Chen Yinghua Jin Jiawei Xu Mai-He Li Hong S.He Ming Jiang 《Forest Ecosystems》 SCIE CSCD 2024年第1期42-51,共10页
Alpine treeline ecotones are highly sensitive to climate warming.The low temperature-determined alpine treeline is expected to shift upwards in response to global warming.However,little is known about how temperature ... Alpine treeline ecotones are highly sensitive to climate warming.The low temperature-determined alpine treeline is expected to shift upwards in response to global warming.However,little is known about how temperature interacts with other important factors to influence the distribution range of tree species within and beyond the alpine treeline ecotone.Hence,we used a GF-2 satellite image,along with bioclimatic and topographic variables,to develop an ensemble suitable habitat model based on the species distribution modeling algorithms in Biomod2.We investigated the distribution of suitable habitats for B.ermanii under three climate change scenarios(i.e.,low(SSP126),moderate(SSP370)and extreme(SSP585)future emission trajectories)between two consecutive time periods(i.e.,current-2055,and 2055-2085).By 2055,the potential distribution range of B.ermanii will expand under all three climate scenarios.The medium and high suitable areas will decline under SSP370 and SSP585scenarios from 2055 to 2085.Moreover,under the three climate scenarios,the uppermost altitudes of low suitable habitat will rise to 2,329 m a.s.l.,while the altitudes of medium and high suitable habitats will fall to 2,201 and2,051 m a.s.l.by 2085,respectively.Warming promotes the expansion of B.ermanii distribution range in Changbai Mountain,and this expansion will be modified by precipitation as climate warming continues.This interaction between temperature and precipitation plays a significant role in shaping the potential distribution range of B.ermanii in the alpine treeline ecotone.This study reveals the link between environmental factors,habitat distribution,and species distribution in the alpine treeline ecotone,providing valuable insights into the impacts of climate change on high-elevation vegetation,and contributing to mountain biodiversity conservation and sustainable development. 展开更多
关键词 Biomod2 BIRCH Climate change Climate scenarios Habitat suitability Range shift Treeline species
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PAL-BERT:An Improved Question Answering Model
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作者 Wenfeng Zheng Siyu Lu +3 位作者 Zhuohang Cai Ruiyang wang lei wang Lirong Yin 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第6期2729-2745,共17页
In the field of natural language processing(NLP),there have been various pre-training language models in recent years,with question answering systems gaining significant attention.However,as algorithms,data,and comput... In the field of natural language processing(NLP),there have been various pre-training language models in recent years,with question answering systems gaining significant attention.However,as algorithms,data,and computing power advance,the issue of increasingly larger models and a growing number of parameters has surfaced.Consequently,model training has become more costly and less efficient.To enhance the efficiency and accuracy of the training process while reducing themodel volume,this paper proposes a first-order pruningmodel PAL-BERT based on the ALBERT model according to the characteristics of question-answering(QA)system and language model.Firstly,a first-order network pruning method based on the ALBERT model is designed,and the PAL-BERT model is formed.Then,the parameter optimization strategy of the PAL-BERT model is formulated,and the Mish function was used as an activation function instead of ReLU to improve the performance.Finally,after comparison experiments with traditional deep learning models TextCNN and BiLSTM,it is confirmed that PALBERT is a pruning model compression method that can significantly reduce training time and optimize training efficiency.Compared with traditional models,PAL-BERT significantly improves the NLP task’s performance. 展开更多
关键词 PAL-BERT question answering model pretraining language models ALBERT pruning model network pruning TextCNN BiLSTM
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Efficacy and safety of anlotinib combined with the STUPP regimen in patients with newly diagnosed glioblastoma: a multicenter, single-arm, phase Ⅱ trial
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作者 Shuzhen Lai Peijing Li +15 位作者 Xiaohui Liu Guihong Liu Tieming Xie Xing Zhang Xiaoxuan wang Jing Huang Yiqiang Tang Zhigang Liu Guoping Shen Chaoming Li Fangxiao Lu lei wang Fagui Jiang Caixing Sun Yuanyuan Chen Ming Chen 《Cancer Biology & Medicine》 SCIE CAS CSCD 2024年第5期433-444,共12页
Objective:Glioblastomas are highly vascularized malignant tumors.We determined the efficacy and safety of the anti-angiogenic multi-kinase inhibitor,anlotinib,for a newly diagnosed glioblastoma.Methods:This multicente... Objective:Glioblastomas are highly vascularized malignant tumors.We determined the efficacy and safety of the anti-angiogenic multi-kinase inhibitor,anlotinib,for a newly diagnosed glioblastoma.Methods:This multicenter,single-arm trial(NCT04119674)enrolled 33 treatment-naïve patients with histologically proven glioblastomas between March 2019 and November 2020.Patients underwent treatment with the standard STUPP regimen[fractionated focal irradiation in daily fractions of 1.8-2 Gy given 5 d/w×6 w(total=54-60 Gy)]or radiotherapy plus continuous daily temozolomide(TMZ)(75 mg/m^(2)of body surface area/d,7 d/w from the first to the last day of radiotherapy),followed by 6 cycles of adjuvant TMZ(150-200 mg/m^(2)×5 d during each 28-d cycle)plus anlotinib(8 mg/d on d 1-14 of each 3-w cycle for 2 cycles during concomitant chemoradiotherapy,8 maximal cycles as adjuvant therapy,followed by maintenance at 8 mg/d.The primary endpoint was progression-free survival(PFS).Secondary endpoints included overall survival(OS)and adverse events(AEs).Results:Thirty-three patients received the planned treatment.The median PFS was 10.9 months(95%CI,9.9-18.7 months)and the 12-month PFS rate was 48.5%.The median OS was 17.4 months(95%CI,14.5-21.1 months)and the 12-month OS rate was 81.8%.The most common AEs included hypertriglyceridemia[58%(n=19)],hypoalbuminemia[46%(n=15)],and hypercholesterolemia[46%(n=15)]during concurrent chemoradiotherapy and leukopenia[73%(n=24)],hypertriglyceridemia[67%(n=22)],and neutropenia[52%(n=17)]during adjuvant therapy.Five patients discontinued treatment due to AEs.HEG1(HR,5.6;95%CI,1.3-23.7;P=0.021)and RP1L1 alterations(HR,11.1;95%CI,2.2-57.2;P=0.004)were associated with a significantly shorter PFS.Conclusions:Anlotinib plus the STUPP regimen has promising anti-tumor activity against glioblastoma and manageable toxicity.HEG1 and RP1L1 alterations might be novel predictive biomarkers of the response to anlotinib. 展开更多
关键词 GLIOBLASTOMA ANTI-ANGIOGENESIS multi-kinase inhibitor anlotinib TEMOZOLOMIDE progression-free survival
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Effect of long reaction distance on gas composition from organic-rich shale pyrolysis under high-temperature steam environment
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作者 lei wang Rui Zhang +4 位作者 Guoying wang Jing Zhao Dong Yang Zhiqin Kang Yangsheng Zhao 《International Journal of Coal Science & Technology》 EI CAS CSCD 2024年第3期102-119,共18页
When high-temperature steam is used as a medium to pyrolyze organic-rich shale,water steam not only acts as heat transfer but also participates in the chemical reaction of organic matter pyrolysis,thus affecting the g... When high-temperature steam is used as a medium to pyrolyze organic-rich shale,water steam not only acts as heat transfer but also participates in the chemical reaction of organic matter pyrolysis,thus affecting the generation law and release characteristics of gas products.In this study,based on a long-distance reaction system of organic-rich shale pyrolysis via steam injection,the effects of steam temperature and reaction distance on gas product composition are analyzed in depth and compared with other pyrolysis processes.The advantages of organic-rich shale pyrolysis via steam injection are then evaluated.The volume concentration of hydrogen in the gas product obtained via the steam injection pyrolysis of organic-rich shale is the highest,which is more than 60%.The hydrogen content increases as the reaction distance is extended;however,the rate of increase changes gradually.Increasing the reaction distance from 800 to 4000 mm increases the hydrogen content from 34.91%to 69.68%and from 63.13%to 78.61%when the steam temperature is 500℃ and 555℃,respectively.However,the higher the heat injection temperature,the smaller the reaction distance required to form a high concentration hydrogen pyrolysis environment(hydrogen concentration>60%).When the steam pyrolysis temperature is increased from 500℃ to 555℃,the reaction distance required to form a high concentration of hydrogen is reduced from 3800 to 800 mm.Compared with the direct retorting process,the volume concentration of hydrogen obtained from high-temperature steam pyrolysis of organic-rich shale is 8.82 and 10.72 times that of the commonly used Fushun and Kivite furnaces,respectively.The pyrolysis of organic-rich shale via steam injection is a pyrolysis process in a hydrogen-rich environment. 展开更多
关键词 Steam temperature Pyrolysis gas Hydrogen-rich Reaction distance Direct retorting
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Understanding mechanisms for differential salinity tissue tolerance between quinoa and spinach:Zooming on ROS-inducible ion channels
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作者 Mohsin Tanveer lei wang +3 位作者 Liping Huang Meixue Zhou Zhong-Hua Chen Sergey Shabala 《The Crop Journal》 SCIE CSCD 2024年第5期1357-1368,共12页
Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importa... Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importance are plant's ability to regulate redox balance without compromising reactive oxygen species(ROS)signalling and maintain cytosolic ion homeostasis.In this study,the mechanistic basis of H^(+)retention ability in leaf mesophyll(an important but highly sensitive plant tissue)was compared between halophytic quinoa and glycophytic spinach.Phenotypic data showed quinoa outperformed spinach under 100 to 500 mmol L^(-1)NaCl salinity.The major difference behind this differential salinity sensitivity was a differential H^(+)uptake in leaf mesophyll.Electrophysiological and molecular experiments revealed that a superior ability of mesophyll H^(+)retention in quinoa was conferred by three complementary mechanisms:(ⅰ)an intrinsically lower H^(+)-ATPase activity in quinoa(potentially as an energy saving strategy);(ⅱ)reduced sensitivity of H^(+)transporters to ROS;and(ⅲ)increased sensitivity of ROS-inducible Ca~(2+)-permeable channels,Moreover,the sensitivity of H^(+)-transport systems to ROS was further examined in NaCl-acclimated quinoa and spinach plants.The key factors differentiating between H^(+)retention in acclimated leaf mesophyll was associated with the reduced sensitivity and gene expression of H^(+)-permeable outward rectifying channel(GORK),Arabidopsis potassium transporter 1(AKT1),and high affinity potassium transporter 5(HAK5)to additional NaCl and ROS stress,along with the upregulation of ROS scavenging system.Taken together,our results showed that the tissue-specific and ROS-specific regulation of H^(+)retention are important for conferring salinity tolerant at least in the halophyte quinoa. 展开更多
关键词 ACCLIMATION Membrane potential ROS signalling Leaf mesophyll Chenopodium quinoa Spinacia oleracea
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A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E
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作者 Huanhuan Lü Pinpin Ji +6 位作者 Siyu Liu Ziwei Zhang lei wang Yani Sun Baoyuan Liu Lizhen wang Qin Zhao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第4期1354-1368,共15页
Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry worldwide.PRV glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA... Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry worldwide.PRV glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some countries.Nanobody has the advantages of small size and easy genetic engineering and has been a promising diagnostic reagent.However,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE antibodies.In the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian camel.Then,two nanobodies against PRV-gE were screened from the immunized camel by phage display technique.Subsequently,two nanobody-HRP fusion proteins were expressed with HEK293T cells.The PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE antibodies.Through optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:5.The cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,respectively.By detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV strains.Simple,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were developed.The bELISA could be used for monitoring and eradicating PR. 展开更多
关键词 NANOBODY nanobody-HRP blocking ELISA PRV-gE ANTIBODY
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High donor-number and low content electrolyte additive for stabilizing zinc metal anode
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作者 Yuxin Gong Ruifan Lin +9 位作者 Bo wang Huaizheng Ren lei wang Han Zhang Jianxin wang Deyu Li Yueping Xiong Dianlong wang Huakun Liu Shixue Dou 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第8期626-635,I0014,共11页
The aqueous zinc ion batteries(AZIBs)are thought as promising competitors for electrochemical energy storage,though their wide application is curbed by the uncontrollable dendrite growth and gas evolution side reactio... The aqueous zinc ion batteries(AZIBs)are thought as promising competitors for electrochemical energy storage,though their wide application is curbed by the uncontrollable dendrite growth and gas evolution side reactions.Herein,to stabilize both zinc anodes and water molecules,we developed a modified electrolyte by adding a trace amount of N,N-diethylformanmide(DEF)into the ZnSO_(4)electrolyte for the first time in zinc ion batteries.The effectiveness of DEF is predicted by the comparison of donor number and its preferential adsorption behavior on the zinc anode is further demonstrated by several spectroscopy characterizations,electrochemical methods,and molecular dynamics simulation.The modified electrolyte with 5%v.t.DEF content can ensure a stable cycling life longer than 3400 h of Zn‖Zn symmetric cells and an ultra-reversible Zn stripping/plating process with a high coulombic efficiency of 99.7%.The Zn‖VO_(2)full cell maintains a capacity retention of 83.5%and a 104 mA h g^(-1)mass capacity after 1000cycles.This work provides insights into the role of interfacial adsorption behavior and the donor number of additive molecules in designing low-content and effective aqueous electrolytes. 展开更多
关键词 Aqueous zinc ion batteries Zinc anode Electrolyte additives Donor number Zinc dendrites
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Insight into the synergistic effect of defect and strong interface coupling on ZnIn_(2)S_(4)/CoIn_(2)S_(4)heterostructure for boosting photocatalytic H_(2) evolution
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作者 Xuehua wang Tianyu Shi +6 位作者 Xianghu wang Aili Song Guicun Li lei wang Jianfeng Huang Alan Meng Zhenjiang Li 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第5期151-161,共11页
Steering the directional carrier migration across the interface is a central mission for efficient photocatalytic reactions.In this work,an atomic-shared heterointerface is constructed between the defect-rich ZnIn_(2)... Steering the directional carrier migration across the interface is a central mission for efficient photocatalytic reactions.In this work,an atomic-shared heterointerface is constructed between the defect-rich ZnIn_(2)S_(4)(HVs-ZIS)and CoIn_(2)S_(4)(CIS)via a defect-guided heteroepitaxial growth strategy.The strong interface coupling induces adequate carriers exchanging passageway between HVs-ZIS and CIS,enhancing the internal electric field(IEF)in the ZnIn_(2)S_(4)/CoIn_(2)S_(4)(HVs-ZIS/CIS)heterostructure.The defect structure in HVs-ZIS induces an additional defect level,improving the separation efficiency of photocarriers.Moreover,promoted by the IEF and intimate heterointerface,photogenerated electrons trapped by the defect level can migrate to the valence band of CIS,contributing to massive photogenerated electrons with intense reducibility in HVs-ZIS/CIS.Consequently,the HVs-ZIS/CIS heterostructure performs a boosted H_(2)evolution activity of 33.65 mmol g^(-1)h^(-1).This work highlights the synergistic effects of defect and strong interface coupling in regulating carrier transfer and paves a brave avenue for constructing efficient heterostructure photocatalysts. 展开更多
关键词 Defect-guided heteroepitaxial growth Heterointerface coupling Photocatalytic H_(2)evolution Mechanism HVs-ZIS/CIS
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Defects and morphology engineering for constructing V_(s)-Ni_(3)S_(2)@V_(s)-Cu_(2)S nanotube heterojunction arrays toward efficient bifunctional electrocatalyst for overall water splitting
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作者 Wenyuan Sun Alan Meng +4 位作者 lei wang Guicun Li Jinfeng Cui Yongkai Sun Zhenjiang Li 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第7期29-40,共12页
The development of highly active,stable and inexpensive electrocatalysts for hydrogen production by defects and morphology engineering remains a great challenge.Herein,S vacancies-rich Ni_(3)S_(2)@Cu_(2)S nan-otube he... The development of highly active,stable and inexpensive electrocatalysts for hydrogen production by defects and morphology engineering remains a great challenge.Herein,S vacancies-rich Ni_(3)S_(2)@Cu_(2)S nan-otube heterojunction arrays were in-situ grown on copper foam(V_(s)-Ni_(3)S_(2)@V_(s)-Cu_(2)S NHAs/CF)for efficient electrocatalytic overall water splitting.With the merits of nanotube arrays and efficient electronic mod-ulation drived by the OD vacancy defect and 2D heterojunction defect,the resultant V_(s)-Ni_(3)S_(2)@V_(s)-Cu_(2)S NHAs/CF electrocatalyst exhibits excellent electrocatalytic activity with a low overpotential of 47 mV for the hydrogen evolution reaction(HER)at 10 mA cm^(-2) current density,and 263 mV for the oxygen evolution reaction(OER)at 50 mA cm^(-2) current density,as well as a cell voltage of 1.48 V at 10 mA cm^(-2).Moreover,the nanotube heterojunction arrays endows V_(s)-Ni_(3)S_(2)@V_(s)-Cu_(2)S NHAs/CF with outstanding stability in long-term catalytic processes,as confirmed by the continuous chronopotentiom-etry tests at current densities of 10 mA cm^(-2) for 100 h. 展开更多
关键词 Nanotubearrays HETEROJUNCTION VACANCY Bifunctional electrocatalyst Overall water splitting
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MOFs‑Derived Strategy and Ternary Alloys Regulation in Flower‑Like Magnetic‑Carbon Microspheres with Broadband Electromagnetic Wave Absorption
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作者 Mengqiu Huang Bangxin Li +7 位作者 Yuetong Qian lei wang Huibin Zhang Chendi Yang Longjun Rao Gang Zhou Chongyun Liang Renchao Che 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第11期479-493,共15页
Broadband electromagnetic(EM)wave absorption materials play an important role in military stealth and health protection.Herein,metal–organic frameworks(MOFs)-derived magnetic-carbon CoNiM@C(M=Cu,Zn,Fe,Mn)microspheres... Broadband electromagnetic(EM)wave absorption materials play an important role in military stealth and health protection.Herein,metal–organic frameworks(MOFs)-derived magnetic-carbon CoNiM@C(M=Cu,Zn,Fe,Mn)microspheres are fabricated,which exhibit flower-like nano–microstructure with tunable EM response capacity.Based on the MOFs-derived CoNi@C microsphere,the adjacent third element is introduced into magnetic CoNi alloy to enhance EM wave absorption performance.In term of broadband absorption,the order of efficient absorption bandwidth(EAB)value is Mn>Fe=Zn>Cu in the CoNiM@C microspheres.Therefore,MOFs-derived flower-like CoNiMn@C microspheres hold outstanding broadband absorption and the EAB can reach up to 5.8 GHz(covering 12.2–18 GHz at 2.0 mm thickness).Besides,off-axis electron holography and computational simulations are applied to elucidate the inherent dielectric dissipation and magnetic loss.Rich heterointerfaces in CoNiMn@C promote the aggregation of the negative/positive charges at the contacting region,forming interfacial polarization.The graphitized carbon layer catalyzed by the magnetic CoNiMn core offered the electron mobility path,boosting the conductive loss.Equally importantly,magnetic coupling is observed in the CoNiMn@C to strengthen the magnetic responding behaviors.This study provides a new guide to build broadband EM absorption by regulating the ternary magnetic alloy. 展开更多
关键词 Magnetic-carbon microspheres MOFs derivatives Electromagnetic wave absorption Magnetic loss Broadband absorption
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A non-probabilistic reliability topology optimization method based on aggregation function and matrix multiplication considering buckling response constraints
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作者 lei wang Yingge LIU +2 位作者 Juxi HU Weimin CHEN Bing HAN 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2024年第2期321-336,共16页
A non-probabilistic reliability topology optimization method is proposed based on the aggregation function and matrix multiplication.The expression of the geometric stiffness matrix is derived,the finite element linea... A non-probabilistic reliability topology optimization method is proposed based on the aggregation function and matrix multiplication.The expression of the geometric stiffness matrix is derived,the finite element linear buckling analysis is conducted,and the sensitivity solution of the linear buckling factor is achieved.For a specific problem in linear buckling topology optimization,a Heaviside projection function based on the exponential smooth growth is developed to eliminate the gray cells.The aggregation function method is used to consider the high-order eigenvalues,so as to obtain continuous sensitivity information and refined structural design.With cyclic matrix programming,a fast topology optimization method that can be used to efficiently obtain the unit assembly and sensitivity solution is conducted.To maximize the buckling load,under the constraint of the given buckling load,two types of topological optimization columns are constructed.The variable density method is used to achieve the topology optimization solution along with the moving asymptote optimization algorithm.The vertex method and the matching point method are used to carry out an uncertainty propagation analysis,and the non-probability reliability topology optimization method considering buckling responses is developed based on the transformation of non-probability reliability indices based on the characteristic distance.Finally,the differences in the structural topology optimization under different reliability degrees are illustrated by examples. 展开更多
关键词 BUCKLING topology optimization aggregation function uncertainty propagation analysis non-probabilistic reliability
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