Bioinformatics and Expression Pattern Analysis of Tomato ns LTP 2-like cDNA full-length Gene Clone

TDF1(transcription-drived fragment) was homologous to the predicted S. lycopersicum nonspecific lipid-transfer protein,nsLTP 2-like(91%), and it was significantly upregulated in response to C. fulvum(cladosporium fulvum) infection in tomato plants.In this experiment, the full-length cDNA of nsLTP 2-like was cloned using RACE technology based on the sequence of TDF1(GenBank: JZ717725). A full-length, 625 bp(GenBank: KU366289), cDNA sequence, which with 98% similarity to nsLTP 2-like gene(GenBank: XM015233692) was obtained. This cDNA contains an ORF(open reading frame) with full-length of 345 bp, coding of 114 amino acids, including 12.3% Ala and Gly. Protein molecular weight was 11.51 ku, the isoelectric point(pI) was 8.99, and average overall hydrophilicity was 0.412, with one phosphorylation sites, belonging to volatile acidic nuclear protein. Secondary structure prediction showed that α-Helix accounts for 30.7%, extension chain for 12.28%, β-corner for 9.65%, and random coil for 47.37%. Through comparative analysis of the homology among species, it was found that the amino acid sequence of tomato nsLTP 2-like protein had a high similarity with other plants, and with a specific conserved sequence which might related features in nsLTP 2-like protein. It also be analyzed the gene expression pattern of tomato in different parts and under different stress conditions.The results showed that nsLTP 2-like gene was up-regulated in varying degrees, under the condition of cold stress, exogenous hormone spraying and cladosporium fulvum infection. Therefore, it was speculated that the gene played a role in response to abiotic and biotic stress in tomato.

Development of Molecular Marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.

重庆市大中专学生艾滋病健康教育干预研究效果探讨

目的通过探讨防治艾滋病健康教育干预研究成效与潜在影响因素,为提升艾滋病防治宣传技术提供科学合理的依据与建议。方法基于自编问卷,对重庆市66所大中专院校青年学生开展防艾健康教育干预性研究,并运用词云图、语义网络分析、情感分析等定性分析,辅以常规定量分析进行干预成效与影响因素探讨。结果定性分析发现,可能影响防治艾滋病健康教育效果的因素主要包括参与方式、反馈途径与交流习惯。定量分析显示,干预前,被调查者中的本科学生艾滋病知识知晓率为78.42%,非本科学生知晓率为66.01%(P<0.001);主城区学生艾滋病知识知晓率为74.61%,非主城区学生知晓率为66.97%(P<0.001);干预后,被调查者艾滋病知识整体知晓率由70.10%提升至83.66%(P<0.001),不同学历被调查者的艾滋病知识知晓率仍差异具有统计学意义(P<0.001),而不同区域被调查者艾滋病知识知晓率差异无统计学意义(P=0.271)。结论充分的防艾健康教育干预在一定程度上能提高艾滋病防治知识的知晓率,同时保持宣教内容与形式的与时俱进,势必将更好推动广大青年学生的防艾宣教工作,切实助力健康中国新国策。

双层异构网络中基于有限反馈的空时干扰对齐研究

本文针对双层异构网络,提出一种利用当前和过期发射端信道状态信息(Channel State Information at the Transmitters,CSIT)的异构空时干扰对齐(Heterogeneous Space-Time Interference Alignment,HSTIA)方案.该方案将空时干扰对齐拓展至双层异构网络MISO(Multiple Input Single Output)混合信道,研究可达自由度关于CSI(Channel State Information)反馈时延的折中域.结果表明,增加发射端天线数能有效提高可达自由度.基于该结论,将空时干扰对齐技术扩展至双层异构网络MIMO(Multiple Input Multiple Output)混合信道,分析MIMO系统发射端与接收端天线数比对系统自由度的影响,并进一步研究系统达到该自由度时CSI反馈时延的取值范围.结果表明,当发射端与接收端天线数比足够多时,系统自由度的稳健性随接收端天线数的增加而加强.通过仿真分析验证,HSTIA方案利用本地当前和过期CSIT执行发射预编码,实现不同时隙的干扰信号相互消除,从而获得自由度增益,以提高系统的可达速率.

Downregulation of SL-ZH13 transcription factor gene expression decreases drought tolerance of tomato

Zinc finger-homeodomain proteins(ZF-HDs) are transcription factors that regulate plant growth,development,and abiotic stress tolerance.The SL-ZH13 gene was found to be significantly upregulated under drought stress treatment in tomato(Solanum lycopersicum) leaves in our previous study.In this study,to further understand the role that the SL-ZH13 gene plays in the response of tomato plants to drought stress,the virus-induced gene silencing(VIGS) method was applied to downregulate SL-ZH13 expression in tomato plants,and these plants were treated with drought stress to analyze the changes in drought tolerance.The SL-ZH13 silencing efficiency was confirmed by quantitative real-time PCR(qRT-PCR) analysis.In SL-ZH13-silenced plants,the stems wilted faster,leaf shrinkage was more severe than in control plants under the same drought stress treatment conditions,and the mean stem bending angle of SL-ZH13-silenced plants was smaller than that of control plants.Physiological analyses showed that the activity of superoxide dismutase(SOD) and peroxidase(POD) and the content of proline(Pro) in SL-ZH13-silenced plants were lower than those in control plants after 1.5 and 3 h of drought stress treatment.The malondialdehyde(MDA) content in SL-ZH13-silenced plants was higher than that in control plants after 1.5 and 3 h of drought stress treatment,and H2O2 and O2^-· accumulated much more in the leaves of SL-ZH13-silenced plants than in the leaves of control plants.These results suggested that silencing the SL-ZH13 gene affected the response of tomato plants to drought stress and decreased the drought tolerance of tomato plants.

2017年重庆市15岁及以上成人烟草流行现状调查研究

目的了解重庆市成人烟草流行现状,为本市采取有针对性的控烟措施、制定相关控烟政策提供依据。方法采用多阶段整群随机抽样法,在全市16个区县共抽取了7802户家庭,以入户调查的形式进行成人烟草调查(TQS),共调查6478人。运用SPSS21.0软件,在复杂调查数据分析模块进行数据分析。结果全市共6478人完成个人调查,重庆市15岁及以上成人现在吸烟率为25.5%,约有645万人现在吸烟。男性吸烟率为48.5%,女性为1.6%,男性吸烟率高于女性;城市吸烟率为24.2%,农村为26.3%。现在吸卷烟者的日平均吸烟量为15.7支。过去1周中,71.4%的成人暴露于二手烟。过去30d内,不同场所暴露于二手烟的比例从高至低依次为:网吧85.2%、酒吧/KTV/夜总会80.1%、餐厅69.2%、室内工作场所42.4%、家中41.8%、大学31.6%、政府大楼28.7%、出租车27.6%、医疗机构26.9%、公共交通工具26.0%和中小学24.6%。结论重庆市15岁及以上成人现在吸烟率低于全国水平(27.7%),但二手烟暴露情况严重,男性吸烟率较高。本市烟草控制面临巨大挑战,应积极采取有效的措施加强控烟工作,制定有针对性的控烟政策,针对不同人群开展多种形式的控烟干预,加强无烟环境创建,逐渐降低本市烟草流行和暴露水平。

三维能量多普勒血流定量技术结合超声弹性成像在早期乳腺癌诊断中的应用价值

目的:探讨三维能量多普勒血流定量技术结合超声弹性成像在早期乳腺癌诊断及临床治疗指导中的应用价值。方法:选择医院收治的经组织病理检验证实的56例早期乳腺癌患者纳入早期乳腺癌组,另选取同期明确诊断为乳腺良性病变的40例患者纳入良性病变组,以及50名体检健康女性纳入健康对照组,3组均接受三维能量多普勒超声以及超声弹性成像检查,对患者乳腺及肿块内血管进行血流定量分析处理,比较乳腺癌与良性病变患者血管相关参数及两种超声检查技术联合应用在早期乳腺癌诊断中的价值。结果:早期乳腺癌患者平均血管形成指数(VI)、血流指数(FI)及血管形成-血流指数(VFI)明显高于良性病变患者,良性病变患者VI、FI及VFI明显高于健康对照组,且3组的弹性成像弹性评分比较,其差异有统计学意义(F=1.142,F=2.843,F=2.599;P<0.05);乳腺癌患者肿块体积越大则肿瘤组织彩色像素密度(CPD)越高,二者具有正性相关关系(r=0.553,P<0.05)。结论:采用三维能量多普勒血流定量技术结合超声弹性成像可实现早期乳腺癌的筛查与诊断,并且二者为临床诊断及治疗提供的信息不同,联合检查通过对相关指标的采集、分析对临床治疗提供指导价值。

Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.In the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)activity.Quantitative real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was downregulated.These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.

Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR

RAPD and SSR were applied to assess genetic diversity in 61 tomato varieties from different species （Solanum lycopersicum L., hirsutum. Humb L., pimpinellifolium Miller L., chilense Dun. L., chmielenskii L., peruvianum Miller L., parvuflorum Miller L.）. 2 062 and 869 clear fragments were amplified by RAPD and SSR, respectively. On the other hand, more polymorphic products were found by SSR as compared to RAPD, i.e., 100 and 43.84%, respectively. In addition, a higher value of the average similarity coefficient and lower PIC value were reflected in RAPD （0.79, 0.407） compared to SSR （0.56, 0.687）. It can be inferred that SSR was a higher effective marker than RAPD to assess genetic diversity in tomato accessions. Similarly, the genetic base of tomato varieties in Chinese market was narrow. It is suggested that wild tomato varieties should be used to enrich the genetic base of the cultivated tomato varieties.

超声心动图血流参数在早产儿动脉导管自然关闭预测中的应用价值

目的:研究超声心动图血流参数指标在预测早产儿动脉导管自然关闭中的应用效果。方法:回顾性分析出生24 h内进行超声心动图检查且存在动脉导管未闭(PDA)的168例早产儿,根据动脉导管关闭情况,将出生8 d内的33例PDA早产儿纳入PDA组,将135例自然关闭早产儿纳入关闭组。两组分别于出生后3 d及8 d对相同早产儿再次行超声心动图检查,对比两组24 h内行超声心动图测得的肺动脉收缩压、卵圆孔分流流速及收缩期肺动脉与降主动脉间的脉压差。结果:PDA组收缩期肺动脉与降主动脉间的脉压差明显低于关闭组,其差异有统计学意义(t=7.51,P<0.05);PDA组肺动脉收缩压明显高于关闭组,其差异有统计学意义(t=5.483,P<0.05);PDA组卵圆孔分流流速明显低于关闭组,其差异有统计学意义(t=6.036,P<0.05)。结论:超声心动图血流参数指标在预测早产儿动脉导管是否自然关闭中有较好的应用效果,具有临床应用价值。

Mapping and Candidate Gene Screening of Tomato Yellow Leaf Curl Virus Resistant Gene ty-5

To identify the inheritance pattern and perform fne mapping of ty-5 gene, P1, P2, F1, BC1 and F2 generations were obtained through a cross between CLN32120a-23 （containing ty-5 gene, P1） and S. lycopersicum Moneymaker （fully susceptible, P2）. The results showed that resistance of ty-5 gene was determined by a recessive effect. Meanwhile, it was presumed that another resistance gene might be involved in mediating the resistance to tomato yellow leaf curl virus （TYLCV）. In this study, fne mapping was used to map TYLCV resistance locus to an interval between NAC1 and TES2461 on the short arm of chromosome 4 with genetic distances of 0.5 and 0.8 cM, respectively. qRT-PCR results showed that four candidate genes, SlNAC1; LOC104229164; LOC101260925 and LOC101261508 having resistance-related expression patterns, were the likely target genes of ty-5. In addition, it was found that the codominant marker TES2461 could be used in marker-assisted selection （MAS） breeding. The fndings of this research provided the basis for future cloning of ty-5 gene as well as MAS breeding and plant resistance mechanism studies.