BACKGROUND Endothelial injury and inflammation are the main pathological changes in hyperuricemic nephropathy(HN);however,they have not been assessed in patients in the early,middle,and late phases of HN.AIM To invest...BACKGROUND Endothelial injury and inflammation are the main pathological changes in hyperuricemic nephropathy(HN);however,they have not been assessed in patients in the early,middle,and late phases of HN.AIM To investigate endothelial injury and inflammatory conditions between patients with HN at chronic kidney disease(CKD)stages 3-4 and CKD 1-2.METHODS This study enrolled 80 patients(49 and 31 with HN at CKD stage 1-2 and 3-4,respectively)from the Department of Nephrology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine between July 2021 and January 2022.Plasma levels of heparan sulfate,endocan,oxidized low-density lipoprotein(Ox-LDL),E-selectin,soluble intercellular adhesion molecule-1(slCAM1),interleukin(IL)-1β,and IL-6 and urine levels of lipocalin-type prostaglandin D synthase(L-PGDS),IL-1β,and IL-6 were measured using enzyme-linked immunosorbnent assay.RESULTS Comparison between patients with HN at CKD 1-2 and those with HN at CKD 3-4 showed that age and disease course were significant factors(P<0.001 and P<0.010,respectively).There were no statistical differences in sex,heart rate,body mass index,and systolic and diastolic blood pressures.The incidence of hypertension was also significant(P=0.03).Plasma levels of heparin sulfate(P<0.001),endocan(P=0.034),E-selectin(P<0.001),slCAM1(P<0.001),IL-1β(P=0.006),and IL-6(P=0.004)and the urine levels of L-PGDS(P<0.001),IL-1β(P=0.003),and IL-6(P<0.001)were high in patients with HN at CKD 3-4 than in those with HN at CKD 1-2.The difference in plasma Ox-LDL levels was not significant(P=0.078).CONCLUSION Vascular endothelial injury and inflammation were higher in patients with HN at CKD3-4 than at CKD 1-2.Plasma heparin sulfate and slCAM1 levels are synergistic factors for CKD staging in HN.展开更多
BACKGROUND Recent epidemiological studies have shown that general eye measurement parameters and corneal biomechanical properties can predict the speed of myopic progression in children.AIM To investigate the correlat...BACKGROUND Recent epidemiological studies have shown that general eye measurement parameters and corneal biomechanical properties can predict the speed of myopic progression in children.AIM To investigate the correlation between the onset and progression of myopia and corneal biomechanical parameters in children.METHODS The study included 102 cases in the emmetropia group,207 cases in the myopic group,and 109 cases in the hyperopic group.The correlation between the change in corneal biomechanical indexes and the change in general ocular measurement parameters was analyzed.A one-way ANOVA test compared general ocular measurement and corneal biomechanical parameters.Pearson’s correlation coefficient was analyzed to correlate corneal biomechanical and general ocular measurement parameters.RESULTS The general ophthalmometric parameters:Spherical equivalent(SE),intraocular pressure(IOP),and axial length(AL),differed significantly among subjects in myopia,emmetropia,and hyperopic groups.Children’s SE positively correlated with corneal biomechanical parameters:Second velocity of applanation(A2V),peak distance(PD),and deformation amplitude(DA)(P<0.05),and second applanation length(A2L)(P<0.05).But it was negatively correlated with PD,DA and integral radius(IR)(P<0.05).Also,IOP was negatively correlated with A2L and IR(P<0.05).AL positively correlated with A2V and negatively correlated with second applanation time(A2T),highest concavity,and PD.Central corneal thickness positively correlated with first applanation length,first applanation time,first applanation deformation amplitude,A2V,A2L,A2T,second applanation deformation amplitude,central curvature radius at highest concavity(HCR),PD,DA,IR,ambrosia relational thickness-horizontal,first applanation stiffness parameter,corvis biomechanical index,topographic and biomechanics index and the first velocity of applanation.The general ocular Km in children positively correlated with corneal biomechanical parameters DA and IR and negatively correlated with A2L,HCR,and PD.There was a positive correlation between the general ocular measurement parametersΔSE and corneal biomechanical parametersΔA2V andΔA2L,and a negative correlation withΔIR.The increase in general ocular measurement parameterΔKm positively correlated with changes in corneal biomechanical parameters,ΔDA andΔIR,and negatively correlated withΔHCR andΔPD.CONCLUSION Myopia development in children was associated with multiple corneal biomechanical parameters.展开更多
BACKGROUND Polycythemia vera(PV),often attributed to the JAK2 V617F mutation,is characterized by enhanced red blood cell counts in the peripheral blood.PV-associated renal disease is clinically rare;to date,there have...BACKGROUND Polycythemia vera(PV),often attributed to the JAK2 V617F mutation,is characterized by enhanced red blood cell counts in the peripheral blood.PV-associated renal disease is clinically rare;to date,there have been reports of other chronic kidney diseases related to PV,but no reports on PV-associated minimal change disease.CASE SUMMARY A 37-year-old man presented with proteinuria and high red blood cell count on January 4,2021.The patient underwent bone marrow and renal biopsies,then was subsequently diagnosed with PV and minimal change in disease.Hydroxyurea was administered and proteinuria remission was achieved.The patient’s last visit was on April 14,2022.CONCLUSION We inferred that there may be a causal relationship between PV and minimal change disease.展开更多
Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a ric...Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a rice leaf wax-deficient mutant osgll-1 derived from a spontaneous mutation, which exhibited a wax-deficient and highly hydro- philic leaf phenotype. We cloned the OsGLI-1 gene by the map-based cloning method and performed a complementation test to confirm the function of the candidate gene. Molecular studies revealed that OsGLI-1 was a member of the OsGL1 family, and contained regions that were homologous to some regions in sterol desaturases and short-chain dehydro- genases/reductases. Compared to the wild-type, the osgll-1 mutant showed decreased cuticular wax deposition, thinner cuticular membrane, decreased chlorophyll leaching, increased rate of water loss, and enhanced sensitivity to drought. OsGL 1-1 is expressed ubiquitously in rice. The transient expression of OsGLl-l-green fluorescent protein fusion protein indicated that OsGLI-1 is localized in the cytoplasm, plasma membrane, and nucleus.展开更多
基金Supported by National Natural Science Foundation of China,No.8187150391 and No.81904126Science and Technology Commission of Shanghai Municipality,No.20Y21901800.
文摘BACKGROUND Endothelial injury and inflammation are the main pathological changes in hyperuricemic nephropathy(HN);however,they have not been assessed in patients in the early,middle,and late phases of HN.AIM To investigate endothelial injury and inflammatory conditions between patients with HN at chronic kidney disease(CKD)stages 3-4 and CKD 1-2.METHODS This study enrolled 80 patients(49 and 31 with HN at CKD stage 1-2 and 3-4,respectively)from the Department of Nephrology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine between July 2021 and January 2022.Plasma levels of heparan sulfate,endocan,oxidized low-density lipoprotein(Ox-LDL),E-selectin,soluble intercellular adhesion molecule-1(slCAM1),interleukin(IL)-1β,and IL-6 and urine levels of lipocalin-type prostaglandin D synthase(L-PGDS),IL-1β,and IL-6 were measured using enzyme-linked immunosorbnent assay.RESULTS Comparison between patients with HN at CKD 1-2 and those with HN at CKD 3-4 showed that age and disease course were significant factors(P<0.001 and P<0.010,respectively).There were no statistical differences in sex,heart rate,body mass index,and systolic and diastolic blood pressures.The incidence of hypertension was also significant(P=0.03).Plasma levels of heparin sulfate(P<0.001),endocan(P=0.034),E-selectin(P<0.001),slCAM1(P<0.001),IL-1β(P=0.006),and IL-6(P=0.004)and the urine levels of L-PGDS(P<0.001),IL-1β(P=0.003),and IL-6(P<0.001)were high in patients with HN at CKD 3-4 than in those with HN at CKD 1-2.The difference in plasma Ox-LDL levels was not significant(P=0.078).CONCLUSION Vascular endothelial injury and inflammation were higher in patients with HN at CKD3-4 than at CKD 1-2.Plasma heparin sulfate and slCAM1 levels are synergistic factors for CKD staging in HN.
文摘BACKGROUND Recent epidemiological studies have shown that general eye measurement parameters and corneal biomechanical properties can predict the speed of myopic progression in children.AIM To investigate the correlation between the onset and progression of myopia and corneal biomechanical parameters in children.METHODS The study included 102 cases in the emmetropia group,207 cases in the myopic group,and 109 cases in the hyperopic group.The correlation between the change in corneal biomechanical indexes and the change in general ocular measurement parameters was analyzed.A one-way ANOVA test compared general ocular measurement and corneal biomechanical parameters.Pearson’s correlation coefficient was analyzed to correlate corneal biomechanical and general ocular measurement parameters.RESULTS The general ophthalmometric parameters:Spherical equivalent(SE),intraocular pressure(IOP),and axial length(AL),differed significantly among subjects in myopia,emmetropia,and hyperopic groups.Children’s SE positively correlated with corneal biomechanical parameters:Second velocity of applanation(A2V),peak distance(PD),and deformation amplitude(DA)(P<0.05),and second applanation length(A2L)(P<0.05).But it was negatively correlated with PD,DA and integral radius(IR)(P<0.05).Also,IOP was negatively correlated with A2L and IR(P<0.05).AL positively correlated with A2V and negatively correlated with second applanation time(A2T),highest concavity,and PD.Central corneal thickness positively correlated with first applanation length,first applanation time,first applanation deformation amplitude,A2V,A2L,A2T,second applanation deformation amplitude,central curvature radius at highest concavity(HCR),PD,DA,IR,ambrosia relational thickness-horizontal,first applanation stiffness parameter,corvis biomechanical index,topographic and biomechanics index and the first velocity of applanation.The general ocular Km in children positively correlated with corneal biomechanical parameters DA and IR and negatively correlated with A2L,HCR,and PD.There was a positive correlation between the general ocular measurement parametersΔSE and corneal biomechanical parametersΔA2V andΔA2L,and a negative correlation withΔIR.The increase in general ocular measurement parameterΔKm positively correlated with changes in corneal biomechanical parameters,ΔDA andΔIR,and negatively correlated withΔHCR andΔPD.CONCLUSION Myopia development in children was associated with multiple corneal biomechanical parameters.
基金Supported by the National Natural Science Foundation of China,No.81874437 and 81904126Science and Technology Commission of Shanghai Municipality,No.20Y21901800.
文摘BACKGROUND Polycythemia vera(PV),often attributed to the JAK2 V617F mutation,is characterized by enhanced red blood cell counts in the peripheral blood.PV-associated renal disease is clinically rare;to date,there have been reports of other chronic kidney diseases related to PV,but no reports on PV-associated minimal change disease.CASE SUMMARY A 37-year-old man presented with proteinuria and high red blood cell count on January 4,2021.The patient underwent bone marrow and renal biopsies,then was subsequently diagnosed with PV and minimal change in disease.Hydroxyurea was administered and proteinuria remission was achieved.The patient’s last visit was on April 14,2022.CONCLUSION We inferred that there may be a causal relationship between PV and minimal change disease.
文摘Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a rice leaf wax-deficient mutant osgll-1 derived from a spontaneous mutation, which exhibited a wax-deficient and highly hydro- philic leaf phenotype. We cloned the OsGLI-1 gene by the map-based cloning method and performed a complementation test to confirm the function of the candidate gene. Molecular studies revealed that OsGLI-1 was a member of the OsGL1 family, and contained regions that were homologous to some regions in sterol desaturases and short-chain dehydro- genases/reductases. Compared to the wild-type, the osgll-1 mutant showed decreased cuticular wax deposition, thinner cuticular membrane, decreased chlorophyll leaching, increased rate of water loss, and enhanced sensitivity to drought. OsGL 1-1 is expressed ubiquitously in rice. The transient expression of OsGLl-l-green fluorescent protein fusion protein indicated that OsGLI-1 is localized in the cytoplasm, plasma membrane, and nucleus.
