Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx 1.2

Homeodomains,a 60-amino acid sequence encoded by 180 nucleotides,are highly conserved DNA-binding motifs that are present in a variety of transcription factors in species ranging from yeast to humans.The NKX proteins belong to the homeodomain(HD)-containing transcription factor family.They play vital roles in the regulation of morphogenesis.NKX1-2 is one member of the NKX subfamily.At present,information about its nuclear localization signal(NLS)sequence is limited.We studied the NLS sequence of zebrafish Nkx1.2 by introducing sequence changes such as deletion,mutation,and truncation,and identified an NLS motif(QNRRTKWKKQ)that is localized at the C-terminus of the homeodomain.Moreover,the deletion of two amino acid residues(RR)in this NLS motif prevents Nkx1.2 from entering the nucleus,indicating that the two amino acids are essential for Nkx1.2 nuclear localization.However,the NLS motif alone is unable to target cytoplasmic protein glutathione S-transferase(GST)to the nucleus.An intact homeodomain is necessary for mediating the complete nuclear transport of cytoplasmic protein.Unlike most nuclear import proteins with short NLS sequences,a long NLS is present in zebrafish Nkx1.2.We also demonstrated that the sequences of homeodomain of NKX1.2 are well conserved among different species.This study is informative to verify the function of the NKX1.2 protein.

Impact of the Rapid Recovery Concept on Complications and Patient Quality of Life in the Perioperative Nursing of Robot-Assisted Radical Oesophageal Cancer

Aim: This study evaluates the impact of Enhanced Recovery After Surgery (ERAS) nursing on postoperative complications and quality of life in patients undergoing robot-assisted minimally invasive esophagectomy (RAMIE). Methods: A total of 150 patients who underwent RAMIE from January 2020 to January 2022 at our hospital were randomly assigned to either the observation group or the control group, with 75 patients in each. The control group received standard perioperative management and nursing care, while the observation group was treated with ERAS nursing strategies. Interventions continued until discharge, and outcomes such as postoperative complications, quality of life, and nutritional status were compared between the groups. Results: The observation group exhibited a significantly lower incidence of postoperative adverse reactions compared to the control group (P ionally, all dimension scores of the Short-Form 36 Health Survey (SF-36), including the total score, were higher in the observation group (P < 0.05). Furthermore, the Nutritional Risk Screening (NRS) scores for impaired nutritional status and disease severity, along with the total NRS score, were significantly lower in the observation group compared to the control group (P Conclusion: Implementing ERAS nursing in the perioperative care of patients undergoing RAMIE is associated with reduced postoperative complications and enhanced postoperative quality of life and nutritional status. .

Prostate cryoablation:A mini review

In recent years,cryotherapy has gained increasing acceptance as a treatment for prostate cancer,offering complementary therapeutic benefits when combined with radical surgery and radiotherapy.Despite the potential for surgical complications,it stands as a safe and viable therapeutic modality.Cryotherapy provides an efficient approach for elderly patients,especially those with compromised physical conditions and individuals experiencing recurrence after initial treatment.It has shown promise in extending survival periods and improving the overall quality of life for these patients.This article aims to comprehensively examine the developmental trajectory,surgical techniques,indications,therapeutic outcomes,and potential complications associated within prostate cancer treatment.

地方高校一流化学专业建设的系统设计与实践

郑州大学化学专业具有悠久的办学历史和优良的办学传统,面对新高考模式对化学专业选材的冲击和拔尖学生培养的新挑战,郑州大学化学学院在人才培养模式方面进行了一系列探索和实践,找到了一条适合地方高校一流专业建设与发展的道路。

组织学分型对于CRS联合HIPEC治疗后的阑尾黏液性肿瘤预后评估的临床意义

目的:阑尾黏液性肿瘤较为罕见,可发生腹膜转移形成腹膜假黏液瘤。目前,临床上多应用肿瘤细胞减灭术(cytoreductive surgery,CRS)和腹腔热灌注化疗(hyperthermic introperitoneal chemotherapy,HIPEC)进行治疗。其组织学类型、腹膜癌指数(peritoneal carcinomatosis index,PCI)评分及细胞减灭程度(completeness of cytoreduction,CC)评分等因素和预后的相关性尚为明确。将规范的组织学分类作为预测因子应用至临床,探讨不同组织学分型、PCI评分和CC评分等因素对伴有腹膜转移的阑尾黏液性肿瘤的预后影响。方法:回顾行分析2009年3月至2019年1月重庆大学附属肿瘤医院就诊的经CRS联合HIPEC治疗后的阑尾黏液性肿瘤的转归。按照2019年第5版世界卫生组织(WHO)对消化道肿瘤推荐的分类标准和国际腹膜表面肿瘤组的规范化组织学分型,采用Cox比例风险模型,通过单变量和多变量分析明确组织学分型、PCI评分、CC评分对患者无进展生存期(progress free survival,PFS)的影响。结果:共48名患者接受了CRS+HIPEC的治疗。经单因素Cox回归分析,PCI评分、CC评分、原发组织学类型和腹膜组织学类型均对PFS存在影响,差异具有统计学意义(P<0.05)。表现为与PCI评分≤10分相比,20~30的危险比为10.38;CC评分与0分相比,1分和3分的危险比分别为4.26和14.74;原发组织学类型中与低级别黏液性肿瘤相比,印戒细胞癌的危险比为9.81;腹膜组织学类型中,与无细胞黏蛋白相比,高级别腹膜黏液癌的危险比为14.35。经多因素Cox回归分析,仅原发组织学类型对PFS存在影响,差异具有统计学意义(P<0.05),原发组织学类型中与低级别黏液性肿瘤相比,印戒细胞癌的危险比为110.79。结论:对于经过CRS+HIPEC治疗阑尾黏液性肿瘤及其引起的腹膜假黏液瘤,规范化地进行原发病灶和腹膜病灶组织学分型对患者预后评估具有重要意义。腹膜病灶组织学恶性程度与原发病灶组织学恶性程度相比呈正相关。原发病灶和腹膜灶恶性度越高,患者预后越差。相对于腹膜病灶组织学分型,原发病灶组织学分型与患者预后更为密切,是更好的预测指标。同时,患者预后与CRS的CC评分和PCI评分有关,PCI评分和CC评分越高,患者预后越差。因此,在CRS+HIPEC治疗中,应对原发病灶和腹膜病灶进行组织学分型,尽可能减瘤彻底,规范化地HIPEC治疗使患者获益。

Preparation and Characterization of Natural Bamboo Fiber

In this study,natural bamboo fiber was prepared combining chemical pretreatment with mechanical disc refining,opening,and carding.An orthogonal experiment was designed based on four factors and three levels;thereafter,the manufacturing process was optimized.The length,diameter,tensile strength,and elastic modulus of the bamboo fiber were determined,and the crystallinity and morphology of the fiber were analyzed using X-ray diffraction(XRD)and scanning electron microscopy(SEM).The results showed that the optimum parameters for the chemical pretreatment were a cooking temperature of 130℃,heating time of 2 h,NaOH dosage of 2%,and Na2SO3 dosage of 10%.The cooking yield of bamboo chips was 89.5%,and the carding yield of natural bamboo fiber was 43.0% under the optimum conditions.The length,diameter,tensile strength,and elastic modulus of the obtained fiber were 36.71 mm,0.285 mm,407 MPa,and 27.7 GPa,respectively.XRD analysis and SEM observations showed that the technology used in this study can produce bright and compact natural bamboo fibers with high crystallinity.

Dynamic Characteristics Analysis of Ice-Adhesion Transmission Tower-Line System under Effect of Wind-Induced Ice Shedding

The tower line system will be in an unsafe status due to uniform or uneven fall of ice coating which is attached to the surface of tower and lines.The fall of ice could be caused by wind action or thermal force.In order to study the dynamic characteristics of the self-failure of the transmission line under the action of dynamicwind load,a finite elementmodel of the two-span transmission tower line system was established.The birth and death element methods are used to simulate the icing and shedding of the line.Tensile failure strength is the shedding criterion for ice coating.The fluctuating wind speed time history of the tower line systemis first simulated,and then the fluctuating wind and the average wind are superimposed to generate the instantaneous wind speed and converted into wind load.The dynamic response of the transmission tower line systemunder iced coupling with different wind speeds and different thicknesses of ice coating was studied.This is the first attempt that the coupling dynamic response of the icing shedding and wind load for the transmission tower-line system is discussed in this paper.In addition,the dynamic characteristics of wind are included.In particular,the limiting mechanical conditions are considered.According to the simulation results,it is found:because of the ice shedding,the stress of the conductor changes obviously in the first 20 seconds,and the ground wire changes sharply in the first two seconds;the icing of the conductor(ground)wire is gradually deicing under the action of wind vibration;the displacement of tower top increases with the increase of wind speed and icing thickness.

Green and Effective Ammonium Carbonate-assisted Process for Drying Hemicellulose Obtained through Alkali Extraction of Bleached Bamboo Kraft Pulp

Hemicellulose has a wide range of applications,including that as an emulsifier for the food industry and raw material for the synthesis of bioethanol/biochemicals and biodegradable films.Hemicellulose is usually present as a spent liquor,such as the prehydrolysis liquor of the prehydrolysis kraft dissolving pulp production process and the alkali extraction liquor of the cold caustic extraction of pulp fibers.Due to its dilute nature,hemicellulose needs to be dried for practical utilization,and this is challenging.In this study,cellulose and hemicellulose in a bleached bamboo kraft pulp were separated using an alkali extraction process.Hemicellulose obtained from the extraction liquor was dried by an ammonium carbonate-assisted drying process.The effects of drying time and drying temperature were determined.Structure of the hemicellulose obtained by the ammonium carbonate-assisted drying process was similar to that of original hemicellulose,as revealed by detailed Fourier transform infrared and X-ray diffraction analyses.The novel drying method was more energy efficient and required a shorter drying time than the conventional freeze drying method,and the excellent solubility in alkaline solutions favored the chemical modification of hemicellulose.The dried hemicellulose can be used as a renewable raw material for the preparation of hydrogels and other substances such as bioethanol/biochemicals and biodegradable films.

Cellulose-based UVB-shielding Film with High Visible Transparency

Cellulose-based Ultraviolet B(UVB)-shielding films were prepared by coating SnO2 film onto regenerated cellulose(RGC) film through a magnetron sputtering method. The dependence of the crystalline quality and optical property of the SnO2 film on the sputtering power was systematically studied. High quality SnO2 film was grown on RGC film at an optimum sputtering power of 150 W. The optical properties of the composite film can be tailored by adjusting the thickness of the SnO2 film. The SnO2/RGC composite film exhibited high visible transparency and excellent UVBshielding capacity, which can be used for protection against short-wave radiation.

Closed Solution for Initial Post-Buckling Behavior Analysis of a Composite Beam with Shear Deformation Effect

This paper is focused on the post-buckling behavior of the fixed laminated composite beams with effects of axial compression force and the shear deformation.The analytical solutions are established for the original control equations(that is not simplified)by applying the Maclaurin series expansion,Chebyshev polynomials,the harmonic balance method and the Newton’s method.The validity of the present method is verified via comparing the analytical approximate solutions with the numerical ones which are obtained by the shooting method.The present third analytical approximate solutions can give excellent agreement with the numerical solutions for a wide range of the deformation amplitudes.What’s more,the effect of shear deformation on the post-bucking configuration of the sandwich beam is also proposed.It can be found that the shear angle has a great influence on the post-buckling load of composite beams.Therefore,the model simplifying the shear formation term as small quantity is not accurate for the case of sandwich beam with soft core.

Carbon Nanoparticles for Identifying Lymph Nodes during Surgery in Colorectal Cancer: A Meta-Analysis

Aim: To investigative the efficacy of carbon nanoparticles (CNs) to identify the lymph nodes during radical surgery in colorectal cancer. Method: The MEDLINE, EMBASE and Cochrane Library databases were searched electronically to identify the studies that compared the use of CNs (CN group) with control group in patients undergoing colorectal cancer radical surgery (from January 2009 to November 2018). The primary outcome was the number of retrieved central lymph nodes. Results: This meta-analysis identified 2 randomized controlled trials and 5 non-randomized controlled trials. Compared with the control group, the CN group resulted in an average of 7.16 more lymph nodes removed per patient (WMD = 7.16, 95% CI = 3.76 to 10.57, p < 0.01), 7.26 minutes less required for retrieving lymph nodes (WMD = -7.26, 95% CI = -13.43 to -1.09, p = 0.02), and 15.1 ml less blood loss during operation (WMD = -15.11, 95% CI = -23.15 to -7.06, p < 0.01). Although there was no significant difference in the metastatic lymph nodes between the two groups (OR = 1.02, 95% CI = 0.79 to 1.31, p = 0.87), there was 1.45 times more metastatic lymph of the stained nodes in CN group than in the control group (OR = 1.45, 95% CI = 1.13 to 1.85, p < 0.01). In addition, lymph nodes less than 5 mm were detected significantly more in the CN group than in the control group (OR = 2.15, 95% CI = 1.77 to 2.63, p < 0.01). Conclusions: The technique of CNs labeled lymph node staining in curative colorectal carcinoma is easy and effective, which can improve the retrieved number of lymph nodes, especially for nodes < 5 mm. The black stained lymph node indicates higher risk of metastasis. Further high quality RCT is needed to verify these conclusions.

NIR-responsive Collagen-based Sponge Coated with Polydimethylsiloxane/Candle Soot for Oil-Water Separation

To fabricate an oil-water separation material that is rich in source,eco-friendly,and responsive,in this study,we successfully developed a collagen-based sponge for application to oil-water separation based on a green and facile strategy.In this design,widely-available collagen(COL)was used as the substrate:it was immersed in polydimethylsiloxane(PDMS)suspension with candle soot(CS)nanoparticles,followed by hot curing.The resultant sponge(CS/PDMS-COL)possessed good hydrophobicity with a water contact angle of 148.3°under a low PDMS concentration of 2%.The results from field emission scanning electron microscope,Fourier transform infrared spectrometer,X-ray photoelectron spectrometer,and X-ray diffractometry demonstrated the successful coating of CS and PDMS on the surface of COL substrate.The CS/PDMS-COL can adsorb eight oils,with the adsorption capacity for trichloromethane reaching 95 g/g.With benzene as the target adsorbent,the separation efficiency was maintained at no less than 95%even after recycling 20 times.CS/PDMS-COL was also used to separate oil-in-water emulsion.Moreover,the sponge killed bacteria effectively due to its excellent near-infrared photothermal responsiveness.This study provides new insight into the preparation of facile oil-water separation materials based on naturally occurring biomaterials effortlessly.

Structural Characteristics of Cellulose and Xylan during in vitro Fermentation by Pig Fecal Bacteria

In this study,cellulose and xylan were in vitro fermented by pig fecal bacteria.Rapid fermentation(40 h) and extended fermentation(eight weeks)were performed.The properties and ultra-structure changes of post-fermented solid residues were studied.In the end effluent,acetic acid,propionic acid,and butyric acid were observed to be the principal short-chain fatty acids(SCFAs) produced by anaerobic fermentation.Xylan was more accessible to bacteria than cellulose,leading to higher SCFA and lactic acid production.In addition,the crystalline structure of cellulose changed,leading to 16.3% and42.1% increases in crystallinity index for rapid and extended fermentation,respectively.Through this research,a systematic and advanced method to study the degradation chemistry of cellulose and xylan during fermentation was developed.

3D打印可穿戴微型超级电容器的锯齿状NiCo_(2)S_(4)基电极

应用3D打印技术制备准固态微型超级电容器(MSCs)在可编程结构设计和高质量负载电极制造方面具有固有的优势.然而,缺乏高性能的可打印墨水和厚电极内缓慢的离子传输,对其实际电荷存储能力提出了重大挑战.本文成功开发了一种具有优异流变性能的新型NiCo_(2)S_(4)基纳米复合墨水,并结合直墨书写的3D打印技术合理设计了准固态MSCs的三维结构.得益于牢固锚定在还原氧化石墨烯(rGO)表面的NiCo_(2)S_(4)纳米颗粒和有序的三维微孔,锯齿状厚电极提供了丰富的反应位点并增强了离子传输.因此,三层锯齿状MSCs的面电容高达416.7 mF cm^(-2).在1 mA cm^(-2)的电流密度下,单层、双层和三层电极的锯齿状MSCs的面电容与相对应的网格状MSCs相比,分别增加了127.1%、349.8%和585.9%.本工作为高面电容MSCs的材料和电极结构的跨尺度设计提供了新见解,推动了MSCs在柔性便携式电子设备中的集成应用研究.

Eubacterium rectale Improves the Efficacy of Anti-PD1 Immunotherapy in Melanoma via l-Serine-Mediated NK Cell Activation

Natural killer(NK)cells,as key immune cells,play essential roles in tumor cell immune escape and immunotherapy.Accumulating evidence has demonstrated that the gut microbiota community affects the efficacy of anti-PD1 immunotherapy and that remodeling the gut microbiota is a promising strategy to enhance anti-PD1 immunotherapy responsiveness in advanced melanoma patients;however,the details of the mechanism remain elusive.In this study,we found that Eubacterium rectale was significantly enriched in melanoma patients who responded to anti-PD1 immunotherapy and that a high E.rectale abundance was related to longer survival in melanoma patients.Furthermore,administration of E.rectale remarkably improved the efficacy of anti-PD1 therapy and increased the overall survival of tumor-bearing mice;moreover,application of E.rectale led to a significant accumulation of NK cells in the tumor microenvironment.Interestingly,conditioned medium isolated from an E.rectale culture system dramatically enhanced NK cell function.Gas chromatography-mass spectrometry/ultrahigh performance liquid chromatography-tandem mass spectrometry-based metabolomic analysis showed that l-serine production was significantly decreased in the E.rectale group;moreover,administration of an l-serine synthesis inhibitor dramatically increased NK cell activation,which enhanced anti-PD1 immunotherapy effects.Mechanistically,supplementation with l-serine or application of an l-serine synthesis inhibitor affected NK cell activation through Fos/Fosl.In summary,our findings reveal the role of bacteria-modulated serine metabolic signaling in NK cell activation and provide a novel therapeutic strategy to improve the efficacy of anti-PD1 immunotherapy in melanoma.

带状γ-Fe_(2)O_(3)/ZnO异质结光催化剂光催化降解四环素

针对传统ZnO光催化活性不高的问题,采用Zn(CH3COO)2和FeCl3作为ZnO和Fe2O3的前驱体,水热条件下采用"一锅法"制备带状γ-Fe2O3/ZnO异质结光催化剂,采用XRD、BET比表面积测量仪、TEM、紫外-可见漫反射、电子顺磁共振(EPR)等对其晶体化学结构进行表征。在可见光光源下,探究了不同γ-Fe2O3负载量时γ-Fe2O3/ZnO异质结光催化剂对四环素的光催化降解的效果。研究表明,ZnO负载γ-Fe2O3后比表面积和光照吸收显著改善,禁带宽度有所减小,可见光光照120 min,n(Zn)∶n(Fe)(原子比)为20∶1的γ-Fe2O3/ZnO异质结光催化剂对四环素的降解率高达97.2%,多次重复使用后四环素的降解率保持在95%以上。

纤维素离子凝胶的制备及性能

开发高性能的导电凝胶已经成为推动柔性电子设备进一步发展的基石。以天然纤维素为凝胶骨架,以离子液体为导电介质,通过加热溶解-冷却凝胶化过程可以制备高性能的绿色离子液体-纤维素复合凝胶(CGel)。复合凝胶中的纤维素基体呈现三维网络结构,并包含大量的孔洞,可以有效吸附和储存离子液体,实现离子液体在纤维素基体中的均匀分布。纤维素离子复合凝胶具有较高的模量(G′>G″)、优异的透明度(88%)、良好的电学性能(2.2 mS/cm)及柔韧性。进一步通过高温加热-冷却凝胶化过程能够实现纤维素离子凝胶体系的再循环生产过程。纤维素离子凝胶展现了对水分子的高度敏感性,其电导率正比于工作湿度,有望应用于环境湿度及人体皮肤状况的高效监督检测。

Melt-Lithosphere Interaction Controlled Compositional Variations in Mafic Dikes from Fujian Province,Southeastern China

Late Mesozoic magmatism in southeastern China has been widely considered to be related to the subduction of the Paleo-Pacific Plate.However,it remains controversial whether mafic rocks are derived from the lithosphere or the asthenosphere.Here we present a comprehensive study on mafic dikes from Fujian Province in southeastern China,aiming to understand their source.Two types of mafic rocks have been recognized based on their trace-element features.Type-Ⅰrocks show arc-like trace-elemental characteristics,while type-Ⅱrocks are distinguished by their relatively flat patterns in primitive-mantle-normalized trace-element diagram.Despite such differences between two types of rocks,these mafic dikes show two trends in the plots of^(87)Sr/^(86)Sr(i)versus La/Nb,which can be explained by the influences of crustal contamination and melt-lithospheric mantle interaction,respectively.^(87)Sr/^(86)Sr(i),La/Nb,Sr/Y and Zr/Y ratios of type-I rocks are significantly correlated to the thickness of the underlying lithosphere,and the signals of lithosphere are clearer with increasing lithospheric thickness.This highlights the important influences of melt-lithosphere interaction during their formation.Such observations also indicate that these mafic rocks are more likely to have been originated from the asthenosphere rather than the lithospheric mantle.

Preparation of guanidine-modified starch for antimicrobial paper

Normal paper does not have antimicrobial properties.To impart antimicrobial properties to paper for medical applications,a guanidine-modified starch was prepared via two reaction steps using guanidine hydrochloride(GH)as a modifier,and added to the paper coating formula.FT-IR demonstrated that the GH was successfully grafted onto the starch via the Schiff base reaction.After coating with the modified starch,the antimicrobial performance of the paper against E.coli and S.aureus was evaluated by the disc diffusion method.The results indicated that the paper treated with the guanidine-modified starch exhibited excellent antimicrobial properties against the E.coli and S.aureus.In addition,the dry and wet strength indexes of the coated paper increased by 25%and 100%,respectively,as compared to the control paper.

Lignin extraction and recovery in hydrothermal pretreatment of bamboo

A significant amount of lignin and hemicellulose are dissolved in the hydrothermal treatment of biomass.The hemicellulose can be recovered and utilized for value-added products.The dissolved lignin can undergo depolymerization and condensation reactions,and interferes with the separation and purification process for hemicellulose recovery.This paper investigated the behavior of the lignin extracted from the hydrothermal pretreatment of bamboo and its contributions to the physical characteristics of the hydrolysate.It was found that the turbidity of the hydrolysate was strongly associated with the lignin fragments and suspended long chain hemicelluloses.As the lignin depolymerization and condensation reactions occurred simultaneously,the dissolved lignin fractions in the hydrolysate increased first and then decreased.The molecular weight(MW)of the dissolved lignin fragments ranged from 3342~5611 g/mol,with mainly guaiacyl(G)and syringyl(S)unit in the structure.