Calcium-fortified fresh milk ameliorates postmenopausal osteoporosis via regulation of bone metabolism and gut microbiota in ovariectomized rats

The aging of the global population has made postmenopausal osteoporosis prevention essential;however,pharmacological treatments are limited.Herein,we evaluate the effect of calcium-fortified fresh milk(FM)in ameliorating postmenopausal osteoporosis in a rat model established using bilateral ovariectomy.After 3 months of FM(containing vitamin D,and casein phosphopeptides,1000 mg Ca/100 g)or control milk(110 mg Ca/100 g milk)supplementation,bone changes were assessed using dual-energy X-ray absorptiometry,microcomputed tomography,and bone biomechanical testing.The results revealed that FM can regulate bone metabolism and gut microbiota composition,which act on bone metabolism through pathways associated with steroid hormone biosynthesis,relaxin signaling,serotonergic synapse,and unsaturated fatty acid biosynthesis.Furthermore,FM administration significantly increased bone mineral content and density in the lumbar spine and femur,as well as femoral compressive strength,while improving femoral trabecular bone parameters and microarchitecture.Mechanistically,we found that the effects may be due to increased levels of estrogen,bone formation marker osteocalcin,and procollagen typeⅠN-propeptide,and decreased expression of the bone resorption marker C-telopiptide and tartrate-resistant acid phosphatase 5b.Overall,the findings suggest that FM is a potential alternative therapeutic option for ameliorating postmenopausal osteoporosis.

Longitudinal investigation of mineral composition in human milk and its correlation with infant anthropometric outcomes among Tibetan mother-infant dyads during the first 6 months postpartum

Nutrients in human milk,including minerals,relate growth and development of breast-fed infants.Tibetan mother-infant dyads possess unique characteristics on early nutrition due to their featured long-lasting lifestyle.This study longitudinally investigated the relationship between the mineral composition in human milk and the Z-scores of infants among Tibetan mother-infant dyads during their first 6 months postpartum through a prospective cohort study.The results show that the minerals of Na,Mg,K,Ca,Cu,Zn,and Se were of higher levels in colostrum than other lactation stages.Several minerals were below the recommended values for infants according to Chinese dietary guidelines.Besides,a large proportion of infant Z-scores were below-2 as lactation period continued.Multivariate statistical analysis revealed that classifications and correlations in varying degrees were observed between minerals in human milk and infant Z-scores.These findings will be advantageous for research upon Chinese early nutrition and progress of tailor-made infant formula.

A Prediction-Based Multi-Objective VM Consolidation Approach for Cloud Data Centers

Virtual machine(VM)consolidation aims to run VMs on the least number of physical machines(PMs).The optimal consolidation significantly reduces energy consumption(EC),quality of service(QoS)in applications,and resource utilization.This paper proposes a prediction-basedmulti-objective VMconsolidation approach to search for the best mapping between VMs and PMs with good timeliness and practical value.We use a hybrid model based on Auto-Regressive Integrated Moving Average(ARIMA)and Support Vector Regression(SVR)(HPAS)as a prediction model and consolidate VMs to PMs based on prediction results by HPAS,aiming at minimizing the total EC,performance degradation(PD),migration cost(MC)and resource wastage(RW)simultaneously.Experimental results usingMicrosoft Azure trace show the proposed approach has better prediction accuracy and overcomes the multi-objective consolidation approach without prediction(i.e.,Non-dominated sorting genetic algorithm 2,Nsga2)and the renowned Overload Host Detection(OHD)approaches without prediction,such as Linear Regression(LR),Median Absolute Deviation(MAD)and Inter-Quartile Range(IQR).

Insight to Pyroptosis in Viral Infectious Diseases

Background: Pyroptosis is defined as programmed necrosis executed by gasdermin D or E (GSDMD or GSDME), which punches cellular membrane. Morphologically, pyroptosis is characterized by cell swelling and cell membrane rupture, leading to the release of cellular contents that triggers intense inflammatory response. More and more studies have found that pyroptosis may be involved in the pathogenesis of viral infection, which may be a determinant for inflammation observed in most viral diseases. Objective: This paper aims to summarize the roles of pyroptosis in the pathogenesis of viral infectious diseases and to provide potential drug targets for the treatment of viral diseases, which will contribute to medical research and public health. Measures: This paper mainly summarizes pyroptosis occurring in diseases caused by different viruses, including human immunodeficiency virus, hepatitis virus, enterovirus, influenza virus and dengue fever virus. Meanwhile, the reported mechanism underlying pyroptosis mediating pathogenesis of these viral diseases will also be described. Conclusion: Current studies have shown that pyroptosis is a double-edged sword in viral infectious diseases. On one hand, pyroptosis leads to pathogenic inflammation of many viral infectious diseases which aggravate tissue damage initiated by viral infection, and blocking proptosis usually relieves the inflammation, which exerts therapeutic effects on viral diseases. On the other hand, moderating pyroptosis can contribute to defense against pathogen infection by releasing immune epitopes and inducing antiviral immune response.

社会流动感知和预期如何影响居民幸福感?——公共服务满意度的中介作用和社会公平感的调节作用

民众幸福感的提升,不仅源于经济流动(即相对收入的提高),还源于社会流动(即社会地位的改善)。基于2015年中国综合社会调查(CGSS)数据,采用Bootstrap方法检验社会流动感知和预期对居民幸福感的作用机制,研究发现,社会流动感知和社会流动预期均对居民幸福感存在正向影响;公共服务满意度在社会流动预期与幸福感之间具有遮掩效应,在社会流动感知与居民幸福感之间具有中介效应;社会公平感在社会流动感知与幸福感之间、社会流动预期与幸福感之间、社会流动预期与公共服务满意度之间,均发挥着调节作用。该研究不仅揭示了社会流动感知和预期对幸福感的作用机制,还具有新的公共政策价值,即社会政策的目标定位可从弱势群体向非弱势群体拓展,回应社会流动高预期人群对公共服务治理(尤其是公平性)的关切,从而带来提升民众幸福感和获得感之效。

差序政府信任影响因素及其内涵维度——基于构思导向和扎根理论编码的混合研究

论文通过文献回顾与深度访谈,创新性提出了差序政府信任的五大影响因素,即央地绩效差异感知、地方资源收支偏差感知、地方政策执行偏差感知、媒介倾向以及个体"亲央疏地"倾向,并进一步构建了差序政府信任的影响因素模型。通过对56名普通公民的访谈素材进行扎根理论的三级编码,构建了各影响因素的内涵维度,其中,地方资源收支偏差感知包含公民对地方政府收入行为偏差、地方政府支出行为偏差、地方政府财政公开透明3个维度的感知;地方政策执行偏差感知包含公民对地方政府政策变通执行、政策执行梗阻、政策结果偏差3个维度的感知;央地政府绩效差异感知包含公民对央地政府在人民生活、社会安全、经济发展、公共服务、行政水平、环境保护6个维度的绩效差异感知;媒介倾向包含正面信息传播倾向、负面信息传播倾向2个维度;个体"亲央疏地"倾向包含爱国情感、利益预期差异、领导魅力差异、腐败预期差异、垂直监督期望、服从程度差异6个维度,以期为差序政府信任影响因素的操作化以及模型检验提供前提条件。

Clinical Study of Mesenchymal Stem Cell Treatment for Acute Respiratory Distress Syndrome Induced by Epidemic Influenza A(H7N9) Infection: A Hint for COVID-19 Treatment

2013年,H7N9病毒在哺乳动物宿主之间迅速传播,并具有人与人之间传播的风险。患者表现为重症肺炎、急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)和呼吸衰竭。间充质干细胞(mesenchymal stem cell,MSC)移植在病毒性肺炎的治疗中有广阔的前景,并于2013年作为应急性治疗方法来治疗H7N9感染的ARDS。该研究在单中心开展,为开放式临床研究。根据自愿和知情同意的原则,17例H7N9感染的ARDS患者移植同种异体经血来源的MSC作为治疗组,44例H7N9感染的ARDS患者作为对照组。与对照组相比,MSC移植显著降低了ARDS病死率(MSC治疗组和对照组死亡率分别为17.6%和54.5%)。此外,在对4名MSC移植患者进行为期5年的随访中,未发现MSC移植产生有害影响。总体而言,该研究结果表明MSC移植显著提高了H7N9感染的ARDS的存活率,并为开展临床前和临床应用MSC治疗H7N9感染的ARDS的研究提供了理论基础。由于H7N9和新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)临床表现相似(如ARDS和呼吸衰竭),并继发多器官功能障碍,因此基于MSC的疗法可能为COVID-19所致的ARDS患者获益。

Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha

The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources.

Influence of climate warming and nitrogen deposition on soil phosphorus composition and phosphorus availability in a temperate grassland,China

Climate warming and nitrogen （N） deposition change ecosystem processes, structure, and functioning whereas the phosphorus （P） composition and availability directly influence the ecosystem structure under condi- tions of N deposition. In our study, four treatments were designed, including a control, diurnal warming （DW）, N deposition （ND）, and combined warming and N deposition （WN）. The effects of DW, ND, and WN on P composition were studied by 3~p nuclear magnetic resonance （3~p NMR） spectroscopy in a temperate grassland region of China. The results showed that the N deposition decreased the soil pH and total N （TN） concentration but increased the soil OIsen-P concentration. The solution-state 31p NMR analysis showed that the DW, ND and WN treatments slightly decreased the proportion of orthophosphate and increased that of the monoesters. An absence of myo-inositol phosphate in the DW, ND and WN treatments was observed compared with the control. Furthermore, the DW, ND and WN treatments significantly decreased the recovery of soil P in the NaOH-EDTA solution by 17%-20%. The principal component analysis found that the soil pH was positively correlated with the P recovery in the NaOH-EDTA solution. Therefore, the decreased soil P recovery in the DW and ND treatments might be caused by an indirect influence on the soil pH. Additionally, the soil moisture content was the key factor limiting the available P. The positive correlation of total carbon （TC） and TN with the soil P composition indicated the influence of climate warming and N deposition on the biological processes in the soil P cycling.

Combined effects of habitat and interspec ificinteraction define co-occurrence patterns of sympatric Galliformes

Background:Disentangling the relative importance of environmental variables and interspecific interaction in modulating co-occurrence patterns of sympatric species is essential for understanding the mechanisms of community assembly and biodiversity. For the two sympatric Galliformes, Silver Pheasants (Lophura nycthemera) and Whitenecklaced Partridges (Arborophila gingica), we know little about the role of habitat use and interspecific interactions in modulating their coexistence. Methods:We adopted a probabilistic approach incorporating habitat preference and interspecific interaction using occupancy model to account for imperfect detection,and used daily activity pattern analysis to investigate the cooccurrence pattern of these two sympatric Galliformes in wet and dry seasons. Results: We found that the detection probability of Silver Pheasant and White-necklaced Partridge were related to habitat variables and interspecific interaction. The presence of Silver Pheasant increases the detection probability of White-necklaced Partridge in both the wet and dry season. However, the presence of White-necklaced Partridges increases the detection probability of Silver Pheasants in the wet season, but decreases the probability in the dry season. Further, Silver Pheasants were detected frequently in the sites of high values of enhanced vegetable index (EVI) in both the wet and dry season, and in sites away from human residential settlement in the wet season. Whitenecklaced partridges were mainly detected in low EVI sites. The site use probabilities of two Galliformes were best explained by habitat variables, Silver Pheasants and White-necklaced Partridges preferred steeper areas during the wet and dry season. Both species mainly occurred in low EVI areas during the wet season and occupied sites away from the resident settlement during the dry season. Moreover, the site use probabilities of two species had opposite relationships with forest canopy coverage. Silver Pheasants preferred areas with high forest canopy coverage whereas White-necklaced Partridges preferred low forest canopy coverage in the dry season, and vice versa in the wet season. Species interaction factor (SIF)corroborated weak evidence of the dependence of the site use of one species on that of the other in the either dry or wet season.Temporally, high overlapping of daily activity pattern indicated no significantly temporal niche differentiation between sympatric Galliformes in both wet and dry seasons. Conclusions:Our results demonstrated that the presence of two species influenced the detection probability interactively and there was no temporal partitioning in activity time between Silver Pheasants and White-necklaced Partridges in the wet and dry seasons.The site use probability of two Galliformes was best explained by habitat variables, especially the forest canopy coverage.Therefore, environmental variables and interspecific interaction are the leading drivers regulating the detection and site use probability and promoting co-occurrence of Silver Pheasants and White-necklaced Partridges.

Comparative Study of Global Seismicity on the Hot Engine Belt and the Cooling Seismic Belt—Improvement on Research Ideas of Earthquake Prediction

The study in this paper analyzes and compares the distribution on the global engine active seismic zone and cooling seismic belt basing on the ANSS earthquake catalog from Northern California Earthquake Data Center. An idea of the seismogenesis and earthquake prediction research is achieved by showing the stratigraphic structure in the hot engine belt. The results show that the main engine and its seismic cones are the global seismic activity area, as well as the subject of global geological disaster. Based on the conjecture of other stratum structure, the energy of crustal strong earthquake and volcano activities probably originates from the deep upper mantle. It is suggested that the research on earthquake and volcano prediction should focus on the monitor and analysis on the sub-crustal earthquake activities.

The breeding biology of endemic Spectacled Parrotbill(Sinosuthora conspicillatus) in Lianhuashan National Nature Reserve, Gansu Province, China

Background: Life history traits play critical roles in population survival and evolution.Breeding information should be particularly detailed in order to provide significant insights into the population status and the evolution of other traits.To our knowledge, there is still no information about the breeding biology of Spectacled Parrotbill(Sinosuthora conspicillatus), an endemic parrotbill in China.Methods: We searched the nests, checked all nests found and recorded the information of eggs, nestlings and nest sites of the Spectacled Parrotbill from 2013 to 2015 at Lianhuashan National Nature Reserve in Gansu Province, China.Results: A total of 16 nests were found.Nest trees were artificial young spruces and honeysuckles.Mean nest height was 0.89 ± 0.47 m(n = 16) above the ground level.All nests were cup-shaped and constructed using leaves, fine strips of barks and grasses by both parents.The mean clutch size was 4.42 ± 0.79(n = 12).The eggs were oval in pale blue without speckles, and the mean egg mass was 1.25 ± 0.07 g(n = 27).The egg length was 15.56 ± 0.46 mm(n = 27) and the width was 12.46 ± 0.29 mm(n = 27).Incubation period was 13 days and nestling period was 13–14 days.The breeding success rate was 46%, and among those failed nest, 71% were depredated and 29% were deserted.Conclusion: Detailed life history information about parrotbill is still limited.The breeding biology of Spectacled Parrotbill reported in the present study should be helpful for further research about population, breeding behavior and conservation of this bird.

Sexual dichromatism and assortative mating by multiple plumage color traits in wild Chestnut Thrush

Sex differences in plumage color are common in bird species.Some bird species are regarded as sexually monochromatic in human visual systems,and in recent years,some species are found to be of cryptic(to human)sexual dichromatism by spectrophotometric techniques.However,the functions of plumage color are still less understood in these species.Here,we focused on plumage color traits in the Chestnut Thrush(Turdus rubrocanus),which is considered as a sexually monochromatic bird by human observers.We used spectrometer analyses and avian visual modeling to investigate the color traits of males and females,and whether these color traits are involved in assortative mating.We found that Chestnut Thrush showed sexual dichromatism in bill,throat and wing,and pairs mated assortatively with colorations of throat,chest,crown and wing.We also found that color of tarsus was different between two consecutive years.These results revealed that Chestnut Thrush is sexually dichromatic in the avian visual system,and plumage color traits play important roles in mate choice.

Strong Convergence of an Iterative Method for Generalized Mixed Equilibrium Problems and Fixed Point Problems

In this paper, we introduce a hybrid iterative method for finding a common element of the set of common solutions of generalized mixed equilibrium problems and the set of common fixed points of an finite family of nonexpansive mappings. Furthermore, we show a strong convergence theorem under some mild conditions.

The Effect of Curcumin on Proliferation and Apoptosis in LNCaP Prostate Cancer Cells

OBJECTIVE To observe the effect of curcumin on proliferation and apop-tosis in the prostate cancer LNCaP cell line. METHODS The AXSYMTM system luciferase method was used to examine the effect of various concentratious of curcumin on the content of prostate specific antigen (PSA) in prostate cancer LNCaP cells. A pGL3-PSA luciferase expression vector, containing 640 bp DNA of the PSA gene 5' -promoter region was constructed and transfected into the LNCaP cells with lipofectin. By measuring luciferase activity, the effect of 10 μmol/L, 20 μmol/L, 30 and 40 μmol/L curcumin on the promoter was studied. Effects on cell growth and apoptosis were analyzed by microscopy, the MTT colorimetric assay and flow cytometry. Western-blotting was used to measure expression of the androgen receptor (AR) in the LNCaP cells treated with different concentrations of curcumin. RESULTS The results showed that the expression of PSA was inhibited as curcumin reduced the activity of luciferase. Curcumin also caused a sigificant concentration-dependent decrease in AR expession measured by Western -blotting. Cell growth was inhibited and apoptosis was induced. CONCLUSION By inhibiting AR expression, curcumin reduced the function of the PSA promoter and inhibited PSA protein expression. Curcumin decreased the cellular proliferation and induced apoptosis in LNCaP cells in a concention-dependent manner.

Study on the Seismogenic Mechanism of the Earthquake Mw6.9 in 2014 in the Aegean Sea Seismic Cone

In accordance with the Seismo-Geothermics theory about methods of intracrustal strong earthquake and volcano prediction, we use the ANSS earthquake catalogue from the Northern California earthquake data center and the EMSC earthquake catalogue from the European-Mediterranean Seismological Centre to study the seismic activities of the Turkish Branch Seismic Cone in the Mediterranean Seismic Cone and the following Aegean Sea Seismic Cone, and show reproduction through graphics and animation, the seismogenic process and seismogenic mechanism of the earthquake Mw6.9 on May 24,2014 innorthern Aegean Sea. It was concluded that the energy of strong earthquake of magnitude around7 inAegean Seawas probably from energy transfer and accumulation in deep mantle and incentive lithosphere in the way of wave pattern, and then the strong earthquake occurs suddenly in search of the weak parts of the surface structure. The purpose of this paper is to open a hole in the traditional seismic genesis, and it is beneficial to the further research on the theory and method of earthquake prediction. It is our first attempt to study this case and it needs further examination. In this paper, we divide the Turkish Branch Seismic Cone of the Mediterranean Seismic Cone into 4 tertiary seismic cones, and we show a preliminary seismo-tectonic model of Aegean region. It will be conducive to seismic monitoring and earthquake prediction research inGreece,Turkey,RomaniaandPolandregions. At present, the world’s earthquake prediction has little effect, and it even tends to be not cognitive. Innovative thinking is the only way out.

Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton

Early stage expression of PR10 combined with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR10 proteins during pathogens’ infection, we cloned a Verticillium-induced PR10 (GbPR10-1) gene from cotton (Gossypium barbadense) and compared its expression patterns and domains with other PR10 proteins. Bioinformatics indicated that GbPR10-1 showed the lowest similarity with other 12 different PR10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR10-1 gene instantly up-regulated after infection by V. dahliae in the sea-island cotton plants. GbPR10-1 was also induced by environmental stimulus including heat, submergence and salt, and ethylene but not by ABA and salicylic acid. The GbPR10-1 protein expressed in E. coli BL21 demonstrated that it had a low ribonuclease-like activity in vitro, and could inhibit V. dahliae hyphae growth but not its spores. Comparison analysis of GbPR10-1 (from resistant species) and GhPR10-1 (from susceptible species) responding to V. dahliae infection, only GbPR10-1 gene was strongly induced in the sea-island cotton plants (incompatible response), indicating that PR10-1 genes was linked to resistance signal. In summary, the earlier activation of GbPR10-1 gene, as the index of resistance response, would be aid to block

Chemical Constituents from the Flowers of Dendrobium chrysanthum

This study aimed to investigate the chemical constituents from the flowers of Dendrobium chrysanthum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and ElMS）. [Result] Nine compounds were obtained and identified as stigmasterol , β-sitosterol , linoleic acid , stigmast-4-en-3-one , dibutyl phthalate , moscatilin , ergosterol , ergosterol peroxide and daucosterol . [Conclusion] Al compounds were isolated from its flowers for the first time.

Seismic Activity and Seismic Cone Tectonics on the West Coast of the United States

In the west coast of the United States, there are frequent strong earthquakes and volcanic activities in the crust, high accuracy of the earthquake catalogue, so it is the best choice to study prediction of strong earthquakes and volcanoes, and there are two different types of seismic cone tectonics, volcanic type and intracrustal strong seismicity type, so it becomes the epitome of global earthquake prediction research, rarer. In this paper, according to the data of ANSS earthquake catalogue in the United States, using the Seismo-Geothermic Theory and its methods, the images were processed such as the planar distribution images and the three-dimensional images of the general earthquakes, subcrustal earthquakes, intracrustal strong earthquakes and volcanic eruption and the sequence diagrams of subcrustal earthquakes in the study area, as well as theory explanation of their relations with the San Andreas Fault. According to this idea, the volcanic origin and precursory information of U01 mini seismic cone tectonic were firstly studied, then the causes of intracrustal strong seismicity of U02 mini seismic cone tectonic and their migration rules were studied. The precursory information of M7.1 earthquake on July 6, 2019, was analyzed and summarized in U02 mini seismic cone tectonic, and a basic method for handling similar events in the future was given. In this paper, it thinks strong earthquake and volcanic disaster are from deep mantle heat energy, rather than the result of the independence movement of surface structure. Therefore, it finds the most natural energy of causes of seismicity and volcanic activity and opens a new direction for the prediction research of earthquakes and volcanoes.

Method Research of Earthquake Prediction and Volcano Prediction in Italy

This paper adopts the earthquake catalogue of the European Mediterranean Seismological Centre (EMSC), in accordance with the principles of Seismo-Geothermics Theory and the concept of seismic cone;it discusses the integrity of the earthquake catalogue and the overview of Mediterranean seismic cones;it focuses on the structural details and structural feature of the Italian branch of the Mediterranean seismic cone;it deduces the precursory process of subcrustal earthquake activities before two earthquakes magnitude over 6 and the eruptions of Etna volcano since 2005;then it summarizes the prediction working method of Seismo-Geothermics on estimating the general shell strength, the general period, and the rough location of future earthquake or volcano activities;and finally it discusses and explains some possible problems. The principle and working process of this method were testified in card No. 0419 in 2012, the author’s prediction card, which can apply to predict for intracrustal strong earthquakes and volcano activities within the global twenty four seismic cones. The purpose of this paper is to develop the tools and methods of the prediction of future earthquake and volcano.