Identification of cell surface markers for acute myeloid leukemia prognosis based on multi-model analysis

Given the extremely high inter-patient heterogeneity of acute myeloid leukemia(AML),the identification of biomarkers for prognostic assessment and therapeutic guidance is critical.Cell surface markers(CSMs)have been shown to play an important role in AML leukemogenesis and progression.In the current study,we evaluated the prognostic potential of all human CSMs in 130 AML patients from The Cancer Genome Atlas(TCGA)based on differential gene expression analysis and univariable Cox proportional hazards regression analysis.By using multi-model analysis,including Adaptive LASSO regression,LASSO regression,and Elastic Net,we constructed a 9-CSMs prognostic model for risk stratification of the AML patients.The predictive value of the 9-CSMs risk score was further validated at the transcriptome and proteome levels.Multivariable Cox regression analysis showed that the risk score was an independent prognostic factor for the AML patients.The AML patients with high 9-CSMs risk scores had a shorter overall and event-free survival time than those with low scores.Notably,single-cell RNA-sequencing analysis indicated that patients with high 9-CSMs risk scores exhibited chemotherapy resistance.Furthermore,PI3K inhibitors were identified as potential treatments for these high-risk patients.In conclusion,we constructed a 9-CSMs prognostic model that served as an independent prognostic factor for the survival of AML patients and held the potential for guiding drug therapy.

Multivariate Analysis of Female Stress Urinary Incontinence and Establishment of a Prediction Model

Stress urinary incontinence(SUI)is a symptom of uncontrolled urine outflow that affects millions of women worldwide[1].SUI is a significant healthcare issue that affects the quality of life of women across numerous domains,including social activities,physical health,mental well-being,employment,and sexual life.

Three-Phase Heterojunction NiMo-Based Nano-Needle for Water Splitting at Industrial Alkaline Condition

Constructing heterojunction is an effective strategy to develop high-performance non-preciousmetal-based catalysts for electrochemical water splitting(WS).Herein,we design and prepare an N-doped-carbon-encapsulated Ni/MoO_(2) nano-needle with three-phase heterojunction(Ni/MoO_(2)@CN)for accelerating the WS under industrial alkaline condition.Density functional theory calculations reveal that the electrons are redistributed at the three-phase heterojunction interface,which optimizes the adsorption energy of H-and O-containing intermediates to obtain the best ΔG_(H*) for hydrogen evolution reaction(HER)and decrease the ΔG value of ratedetermining step for oxygen evolution reaction(OER),thus enhancing the HER/OER catalytic activity.Electrochemical results confirm that Ni/MoO_(2)@CN exhibits good activity for HER(ƞ_(-10)=33 mV,ƞ_(-1000)=267 mV)and OER(ƞ_(10)=250 mV,ƞ_(1000)=420 mV).It shows a low potential of 1.86 V at 1000 mA cm^(−2) for WS in 6.0 M KOH solution at 60℃ and can steadily operate for 330 h.This good HER/OER performance can be attributed to the three-phase heterojunction with high intrinsic activity and the self-supporting nano-needle with more active sites,faster mass diffusion,and bubbles release.This work provides a unique idea for designing high efficiency catalytic materials for WS.

Small-signal modeling and parameter extraction method for a multigate GaAs pHEMT switch

This paper presents an accurate small-signal model for multi-gate GaAs pHEMTs in switching-mode.The extraction method for the proposed model is developed.A 2-gate switch structure is fabricated on a commercial 0.5μm AlGaAs/GaAs pHEMT technology to verify the proposed model.Excellent agreement has been obtained between the measured and simulated results over a wide frequency range.

Study of Local Structural Changes on Air Cooling at the End of Rotor Windings for Variable Speed Pumped Storage Generator-Motor

The shrink fit retaining ring is currently the easiest to install and the most widely used end fixed for structure AC excitation variable speed generator-motor rotor end windings.However,the current research on the effect of high strength sealing on the ventilation and heat dissipation performance of the end is not enough.In this paper,based on the actual structural parameters and periodic symmetry simplification,the three-dimensional coupled calculation model of fluid field and temperature field is established.After solving the fluid and thermal equations,the influence of the length of rotor support block,the height of rotor support block,and the number of rotor support block on the fluid flow and temperature distribution in the rotor end region of generator-motor is studied using the finite volume method.The rheological characteristics of the air in the rotor domain,such as velocity and inter-winding flow,are analyzed.The law of temperature variation with local structure in the computational domain is studied.The variation law of cooling medium performance inside the large variable speed power generator motor is revealed.

The Polymorphism and Transformation of (3aRS, 4RS, 7RS, 7aSR)-2-(Tricyclo[3.3.1.13,7]decan-1-yl)-4,5,6,7-tetrahydro-4,7-eposyisoindoline-1,3-dione (SU2162)—A Novel Anticancer Compound

Objective: To determine the transformation between two known crystal forms of the title compound (C18H23NO3, Mr = 301.37). Methods: To recrystallize or heat the crystals and determine the crystal form by testing the melting points. Results: Both the two known crystal forms of the title compound can be changed by dissolving into different organic solvents such as acetone and ethyl acetate. Crystal form I was not influenced by heating while crystal form II can be transformed to crystal form I through melting method. Conclusion: Organic solvents have significant influences on the two crystal forms of title compound. Crystal form I shows a better thermal stability than crystal form II.

Preparation and Characterization of Two Polymorphs of (3aRS,4RS,7RS,7aSR)-2-(Tricyclo[3.3.1.1^3,7]decan-1-yl)-4,5,6,7-tetrahydro-4,7-eposyisoindoline-1,3-dione (SU2162) with PXRD and DSC

Objective: To develop the characterization of the polymorphs and the best preparation method of two forms of the title compound (SU2162). After SU2162 was prepared in accordance with the patent process, the crystal form I was recrystallized by ethyl acetate and the crystal form II was obtained by the recrystal in acetone. And the two crystal forms were characterized with differential scanning calorimetry (DSC) and X-ray powder diffraction (PXRD). The melting point of crystal form I (triclinic) is at 158°C, and the melting point of crystal form II (monoclinic) is at 163°C. The PXRD studies of the two crystalline samples indicate that they have the distinct diffraction patterns. The method herein can be stably prepared for the two crystal forms of the title compound.

Spatiotemporal characteristics of summer hypoxia in Mirs Bay and adjacent coastal waters,South China

Mirs Bay is a semi-enclosed bay neighboring the Zhujiang(Pearl)River estuary,one of the largest estuarine systems in the world.The long-term historical observational data(1994-2017)of temperature,salinity,dissolved oxygen(DO),and biochemical parameters were used to examine the spatiotemporal distribution of hypoxia in Mirs Bay and adjacent coastal waters.Results show that bottom hypoxia varied seasonally and interannually.Hypoxia mainly occurred from June to September in Mirs Bay and the transition zone in the southern waters of Hong Kong,and the recorded hypoxia events have increased from 2007.The density difference between the bottom and surface layers was positively related to the bottom apparent oxygen utilization(AOU)(R=0.620,P<0.001)and negatively related to the bottom DO(R=0.616,P<0.001),indicating that water column stratification was an essential prerequisite for the formation of bottom hypoxia in summer.The bottom oxygen consumption and hypoxia had higher positive correlation with the seasonal thermocline(R=0.683,P<0.001)than the halocline(R=0.540,P<0.001),including in the area was affected by freshwater plume.The insignificant relationship between AOU and nutrients indicated that local eutrophication was not the only important factor in the formation of the hypoxic zone during summer.The decrease in phosphorous owing to the pollutant reduction policy and the increase in nitrate may have led to an increase in hypoxia events in the bay where waters therein are characterized by nitrogen-limitation.The increase in chemical oxygen demand in wastewater also promoted oxygen consumption.Compared to the adjacent coastal waters influenced by Zhujiang River plume water,the Mirs Bay experienced more hypoxia events.The high concentrations of ammonium and total Kjeldahl nitrogen in the sediment of Mirs Bay increased the oxygen depletion in the bottom water.The long residence time of the near-bottom water in Mirs Bay increased the risk of bottom hypoxia events,although the nutrient concentrations were lower than those in the transition zone.These factors lead to differences in hypoxia occurrence in Mirs Bay and adjacent coastal waters.

基于单神经元PID控制器的四旋翼飞行器航迹控制

四旋翼飞行器从起点平滑旋进飞向终点,为控制飞行器稳定飞行,首先建立数学模型和运动力学模型,在此基础上设计双闭环单神经元PID控制器,并根据其动力学特性分析加速度对航迹跟踪,使飞行器飞行轨迹稳定。模型的建立与仿真实验都采用Matlab/Simulink平台,仿真结果证实此方法的可行性。

近红外荧光成像技术在外科手术中的研究进展

作为术中导航的新兴领域,近红外荧光(near-infrared fluorescence,NIRF)成像技术能借助荧光探针对特定的生物组织进行显像,从而在外科手术中实时获取血管、淋巴管和特定组织的视觉信息,从而发现传统方法无法识别的微小灶,正确显示肿瘤切缘,帮助外科医生进行术中决策。该技术由于其操作简单,快速实时,安全无害,具有良好的应用前景。本文将就NIRF成像技术的基本原理,在实时引导肿瘤切除,前哨淋巴结定位,保护正常解剖结构等方面的临床进展进行综述,并且讨论了该技术的局限性和优势并展望其应用前景。

Efficacy of epalrestat plus α-lipoic acid combination therapy versus monotherapy in patients with diabetic peripheral neuropathy: a meta-analysis of 20 randomized controlled trials

OBJECTIVE： To evaluate the efficacy of α-lipoic acid（ALA） plus epalrestat combination therapy in the treatment of diabetic peripheral neuropathy（DPN）. DATA SOURCES： The electronic databases of Pub Med, Medline, Embase, the Cochrane Library, the Chinese National Knowledge Infrastructure, the Wanfang Database and the Chinese Biomedical Database were used to retrieve relevant studies without language restrictions. The search was conducted from the inception of each database to 7 October 2016. The key terms were（diabetic peripheral neuropathy or diabetic neuropathy or DPN） AND（α-lipoic acid or lipoic acid or thioctic acid） AND epalrestat. DATA SELECTION： All of the eligible studies met the following inclusion criteria：（1） Randomized controlled trials that compared efficacy and safety of epalrestat plus ALA combination therapy versus epalrestat or ALA monotherapy in patients with DPN.（2） The minimum duration of treatment was 2 weeks.（3） The DPN patients were diagnosed using the World Health Organization standardized type 2 diabetes mellitus and DPN criteria.（4） Studies contained at least one measure that could reflect the efficacy of the drug and nerve conduction velocities. Studies in which the control group used epalrestat or ALA combined with other drugs were excluded. Statistical analyses were performed using STATA software for meta-analysis. OUTCOME MEASURES： The primary outcomes were the therapeutic efficacy, median motor nerve conduction velocity（MNCV）, median sensory nerve conduction velocity（SNCV）, peroneal MNCV and peroneal SNCV.RESULTS： Twenty studies with 1894 DPN patients were included, including 864 patients in the ALA plus epalrestat group, 473 in the ALA group and 557 in the epalrestat group. The efficacy of ALA plus epalrestat combination therapy was superior to ALA and epalrestat monotherapies（RR = 1.29, 95% CI： 1.21–1.38; RR = 1.43, 95% CI： 1.34–1.54, respectively）. ALA plus epalrestat combination therapy also significantly improved median MNCV（WMD = 5.41, 95% CI： 2.07–8.75）, median SNCV（WMD = 5.87, 95% CI： 1.52–10.22）, peroneal MNCV（WMD = 5.59, 95% CI： 2.70–8.47） and peroneal SNCV（WMD = 4.57, 95% CI： 2.46–6.68）.CONCLUSION： ALA plus epalrestat combination therapy was superior to ALA and epalrestat monotherapies for clinical efficacy and nerve conduction velocities in patients with DPN.

Immune response,stress resistance and bacterial challenge in juvenile rainbow trouts Oncorhynchus mykiss fed diets containing chitosan-oligosaccharides

Effects of dietary supplementation of chitosan-oligosaccharides （COS） on the growth performance, immune response, stress resistance, and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied. Four experimental diets containing 0, 20, 40, or 60 mg/kg COS （COSO, COS20, COS40, and COS60, respectively） were fed to juvenile rainbow trout （initial weight = 5.2 ± 0.3 g） for 8 weeks. By the end of the feeding trial, representative groups of fish from each dietary treatment were challenged with stressor （30 see air exposure） and pathogen exposure （intraperitoneal injection with Aeromonas hydrophila ）. Results showed that supplementation of COS in diets did not affect production performance and body composition of rainbow trout. However, fish fed the COS40 diet demonstrated improved phagocytic activities, respiratory burst activities and decreased serum cortisol level. Additionally, survival following A. hydrophila challenge was significant higher among fish fed the COS-supplemented feeds, although there was no difference based on the level of supplementation. The present study suggests that COS can be used as an immuno-stimulant in rainbow trout feeds

N‑Doped Graphene‑Decorated NiCo Alloy Coupled with Mesoporous NiCoMoO Nano‑sheet Heterojunction for Enhanced Water Electrolysis Activity at High Current Density

Developing highly effective and stable non-noble metalbased bifunctional catalyst working at high current density is an urgent issue for water electrolysis(WE).Herein,we prepare the N-doped graphene-decorated NiCo alloy coupled with mesoporous NiCoMoO nano-sheet grown on 3D nickel foam(NiCo@C-NiCoMoO/NF)for water splitting.NiCo@C-NiCoMoO/NF exhibits outstanding activity with low overpotentials for hydrogen and oxygen evolution reaction(HER:39/266 mV;OER:260/390 mV)at±10 and±1000 mA cm^(−2).More importantly,in 6.0 M KOH solution at 60℃ for WE,it only requires 1.90 V to reach 1000 mA cm−2 and shows excellent stability for 43 h,exhibiting the potential for actual application.The good performance can be assigned to N-doped graphene-decorated NiCo alloy and mesoporous NiCoMoO nano-sheet,which not only increase the intrinsic activity and expose abundant catalytic activity sites,but also enhance its chemical and mechanical stability.This work thus could provide a promising material for industrial hydrogen production.

Numerical investigation on the tensile fracturing behavior of rock-shotcrete interface based on discrete element method

Four groups of numerical models of Brazilian tests on rock-shotcrete interfaces were successfully conducted by PFC2D. The tensile strength and Young’s modulus of shotcrete were considered. Six different undulations of rock-shotcrete interface were set up. The influences of multiple parameters on the bearing characteristics of the rock-shotcrete interface were studied. The results showed that a better support performance can be obtained by increasing the Young’s modulus of shotcrete rather than the tensile strength of shotcrete. For different tensile strength and Young’s modulus, the increase of sawtooth height has different effects on the support performance. The failure mechanism of the rock-shotcrete interfaces was analysed in detail. The stress shielding effect and stress concentration effect caused by the shape characteristics of rock-shotcrete interface were observed. The influence of these parameters on the overall support performance should be fully considered in a reasonable support design.

Enhancement of oxygen reduction activity and stability via introducing acid-resistant refractory Mo and regulating the near-surface Pt content

Although Pt Ni catalyst possesses good oxygen reduction activity, its poor stability is the main obstacle for the commercialization of proton exchange membrane fuel cells(PEMFCs). In this work, we introduce the acid-resistant refractory Mo to enhance the structure stability and modify the electronic structure of Pt in the prepared PtNi catalyst, improving the catalytic activity for oxygen reduction reaction(ORR). In addition, near-surface Pt content in the nanoparticle is also optimized to balance the ORR activity and stability. The electrochemical results show that the alloy formed by Mo and Pt Ni is obviously more stable than the PtNi alloy alone, because the acid-resistant Mo and its oxides effectively prevent the dissolution of Pt. Especially, the Pt3 Ni3 MoN/C exhibits the optimal ORR catalytic performance in O2-saturated 0.1 mol L^(-1) HClO4 aqueous solutions, with mass activity(MA) of 900 m A mg^(-1) Pt at 0.90 V vs. RHE, which is 3.75 times enhancement compared with the commercial Pt/C(240 mA mg^(-1) Pt). After 30 k accelerated durability tests, its MA(690 m A mg^(-1) Pt) is still 2.88 times higher than the pristine Pt/C. This study thus provides a valuable method to design stable ORR catalysts with high efficiency and has great significance for the commercialization of PEMFCs.

Reproductive Tract Infections—A Main Factor Influencing Women’s Mental Status: Comparison on Depression and Anxiety between Naxi Women with and without RTIs

Objectives 1） To explore the relationship between RTIs and mental status of Naxi women; 2） to compare the differences of depression ＆ anxiety between Naxi women who have and not have RTIs; and 3） to put forward some suggestions for improving Naxi women＇s reproductive health in psychological point of view. Methods A cross-sectional survey was adopted, 280 married Naxi female volunteers who aged above 20 years old were selected by cluster random sampling from the two selected villages of Lugufu Township of Yanyuan county in Sichuan. Two self-reporting scales, CES-D and SAS were used for assessment of depression and anxiety of the subjects. Results The facts of mental status of Sichuan Naxi women brook no optimism. Among 280 Naxi reproductive age women who were investigated in current study, only 74 （26.4%） have no depression symptoms, and 116 （41.4%） have no anxiety symptoms. For the study population, the average total scores （TS） of CES-D was 20.1, and the average total index scores （TIS） of SAS was 50.2, and both of them were above a minimum value doubted to have symptoms. There were big differences of both average TS of CES-D and TIS of SAS between Naxi women who have and not have RTIs. Further analysis revealed that RTI was a main risk factor influencing women＇s mental status （OR = 16. 043 for depression, and OR = 12.954 for anxiety）. In addition, Naxi women＇s depression and anxiety were related to order births （≤2, OR=3.149, 95% CI： 1.228, 8.076）, sex debut was younger （≤17, OR=3.043, 95% CI： 1.895, 4.884）, and multiple pregnancy （≥ 3, OR=2.728, 95% CI： 1.990, 4.173）, etc. Conclusion For improving Naxi women＇s mental status, a pressing matter of the moment is for local medical persons to gain the knowledge about mental health and the diagnosis and treatment levels of psychological disorders. At the same time, pssychological counselling should become a main activity of reproductive health services.

Pitch Shift in Exsomatized Cochlea Observed by Laser Interferometry

Pitch is one of the most important auditory perception characteristics of sound; however, the mechanism underlying the pitch perception of sound is unclear. Although theoretical researches have suggested that perception of virtual pitch is connected with physics in cochlea of inner ear, there is no direct experimental observation of virtual pitch processing in the cochlea. By laser interferometry, we observe shift phenomena of virtual pitch in basilar membrane vibration of exsomatized cochlea, which is consistent with perceptual pitch shift observed in psychoacoustic experiments. This means that the complex mechanical vibration of basilar membrane in cochlea plays an important role in pitch information processing during hearing.

Stress concentration factor expression for tension strip with eccentric elliptical hole

Two explicit expressions of the stress concentration factor for a tension finite-width strip with a central elliptical hole and an eccentric elliptical hole, respectively, are formulated by using a semi-analytical and semi-empiricai method. Accuracy of the results obtained from these expressions is better, and application scope is wider, than the results of Durelli＇s photo-elastic experiment and Isida＇s formula. When eccentricity of the elliptical hole is within a certain range, the error is less than 8%. Based on the relation between the stress concentration factor and the stress intensity factor, a stress intensity factor expression for tension strips with a center or an eccentric crack is derived with the obtained stress concentration factor expressions. Compared with the existing formulae and the finite element analysis, this stress intensity factor expression also has sufficient accuracy.

热拉伸温度对聚偏氟乙烯微孔膜结构与性能的影响

采用熔体挤出拉伸法以高、低分子量混合聚偏氟乙烯(PVDF)为原料制备微孔膜,研究了不同热拉伸温度对PVDF微孔膜表面形貌及结晶行为的影响,通过扫描电子显微镜、红外光谱仪、X射线衍射仪和差示扫描量热仪等测试手段分别对微孔膜的形态、结构、晶型进行表征。结果表明,热拉伸温度对PVDF微孔膜的成孔形貌、孔隙率、结晶度及β相含量均有影响。热拉伸温度为80℃时,微孔膜结晶度为58.86%,β相的相对含量最高达到42.50%,此时微孔膜的力学性能最佳;而当热拉伸温度为120℃时,PVDF微孔膜的成孔分布更加均匀,其孔隙率可达到最大值31.51%。

Comparison of Depression and Anxiety between Unmarried Female Youth in Two Groups of Induced Abortion and None-Induced Abortion in Yibin City of China

Objectives To explore the risk factors influencing the mental status of unmarried female youth. Methods ,4 cross-sectional survey and a qualitative interview were adopted. 1200 unmarried female volunteers （600 with 1,4） who aged 15-29 years in Yibin city were investigated, and 8 focus group discussions （FGDs） and 8 in-depth interviews were conducted. Except the investigation of subject＇s personal information, econ-socio status, sexual behavior, contraception and reproductive health problems they face, two selfreporting scales, CES-D and SAS were used for assessment of depression and anxiety of the subjects. Results 1） Depression and anxiety among unmarried female youth investigated in current study are relatively common. Proportions of the females with depression and anxiety to the total sample are 27.4% and 33.4%, respectively; 2） Depression and anxiety of the females with 1,4 are more serious than that of the female without 1,4. Proportions of unmarried females with IA have depression and anxiety to the total unmarried females with 1,4 are 3.45 times and 2.61 times that of unmarried females without 1,4 have depression and anxiety to the total unmarried females without 1,4, respectively; 3） IA is one of the key risk factors influencing depression and anxiety of unmarried female youth. Other risk factors are lack on basic knowledge about sex/ STD/HIV/AIDS, reproductive tract infections, relatively lower income, age at sex debut is relatively younger, etc. In addition, multiple sex partners and repeat pregnancies were risk factors influencing anxiety of unmarried female youth. Conclusion Depession and anxiety was more popular in unmarried females with IA Intervention for improving mental health of unmarried females should be developred Key words：