1型登革病毒感染病人血清中非结构蛋白1B细胞线性表位鉴定

目的鉴定1型登革病毒感染病人血清中1型登革病毒非结构蛋白1(nonstructural Protein 1 of Dengue virus type 1,DENV-1 NS1)B细胞线性表位。方法以重组DENV-1 NS1为抗原,免疫印迹法筛选NS1阳性病人血清。一组重叠DENV-1 NS1的重叠多肽同这些NS1阳性血清反应鉴定NS1蛋白线性B细胞表位。采用梯度稀释的1-4型重组DENVNS1竞争抑制阳性多肽同病人血清反应鉴定其反应特异性和交叉反应性。结果12份DENV-1核酸阳性病人中筛选得到5份抗DENV-1 NS1阳性病人血清。重叠多肽法、竞争抑制法和生物信息学方法筛选出3条阳性NS1多肽(氨基酸残基序列第1-15,131-145,271-285),其中aa1-15在已报道的DENV-1分离株高度保守,提示这个区域可能存在DENV-1血清型特异性表位。aa131-145和aa271-285对应的蛋白序列比对分析表明以上区域中的133-FI/LIDGP-138和271-GKLEL/M/IDF-277在已报道的4种血清型DENV分离株中高度保守,提示这2个区域存在4种血清型DENV共同表位。结论发现3个NS1上B细胞线性表位,其中aa131-145和aa271-285为首次报道,结果有助于疫苗和诊断试剂的研发。

基于竞争配位的Ca2+特异性响应1H/19F磁共振成像分子探针

合成了一种含19F的Mn2+配合物3,12-二(2-氧代-2-((2,2,2-三氟乙基)氨基)乙基)-6,9-二氧杂-3,12-二氮杂十四烷酸锰(Ⅱ)(Mn(Ⅱ)-L,1),可实现对Ca2+特异响应的1H/19F磁共振成像分析。19F核在近距离的顺磁性Mn2+影响下产生了顺磁弛豫增强作用,使19F的横向弛豫时间T2急剧缩短而磁共振信号锐减。当有Ca2+存在时,与配体L的竞争配位使得19F远离Mn2+离子,从而19F磁共振信号得到恢复。同时,由于Mn2+离子从配合态变为游离态,水配位数增加使得其对1H的纵向弛豫时间T1弛豫性能增加,从而1H磁共振成像信号也增强。相关实验的结果证实了该配合物是一种能对Ca2+特异性响应的1H/19F磁共振成像探针。

床旁电子支气管镜在重症肺炎患者治疗中的应用效果

目的:探究重症肺炎实施床旁电子支气管镜治疗的价值。方法:将2021年3月~2022年3月重症肺炎患者作为样本,符合标准并入选90例患者,掷骰子分组,分为参考组、实践组各45例,参考组行常规治疗,实践组加用床旁电子支气管镜治疗,检测血气指标,观察炎症因子,比较肺部感染情况,统计恢复时间,记录有效率。结果:治疗后SpO_(2)、PaO_(2)结果实践组高于参考组,PaCO_(2)结果实践组低于参考组,P<0.05。治疗后三项炎症因子结果实践组均低于参考组,P<0.05。治疗后3d、6d、14d时肺部感染评分结果实践组低于参考组,P<0.05。各项症状恢复时间实践组短于参考组,P<0.05。有效率实践组高于参考组,P<0.05。结论:床旁电子支气管镜有效率高,能改善血气、炎症问题,大幅减轻肺感染程度,加快各重症肺炎症状的消除。

Effects of moxibustion on the P2X7R/STAT3/VEGF pathway in rats with colitis-associated colon cancer

Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibustion intervening CACC through the purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)pathway.Methods:A total of 26 male Sprague-Dawley rats were selected.According to the random number table method,6 rats were selected as the normal group.The remaining 20 rats were injected intraperitoneally with azoxymethane(AOM)combined with oral dextran sodium sulfate(DSS)to prepare the CACC model.After the model was successfully established,2 rats were randomly selected for model identification.The remaining 18 rats which were successfully modeled were randomly divided into a model group,a herb-partitioned moxibustion group and a ginger-partitioned moxibustion group,with 6 rats in each group.Moxibustion intervention was performed in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group at Qihai(CV 6)and bilateral Tianshu(ST 25).Moxibustion was performed twice at each point each time,once a day,at a 1-day interval after 6 consecutive interventions,for a total of 30 interventions.After intervention,the colon tumor load,pathological change and histopathological score were observed.Immunohistochemistry was used to detect the expressions of VEGF,P2X7R,phospho-STAT3(p-STAT3),and nuclear factor-kappa B p65(NF-κB p65)proteins in rat colon tissue.Western blot was used to detect the levels of p-STAT3 and NF-κB p65 proteins in rat colon tissue.Results:Compared with the normal group,the colon tumor load and histopathological score in the model group were significantly increased(both P<0.001),and different grades of dysplasia were observed in colon tissue from the model group,reaching the degree of adenocarcinoma;the expression level of P2X7R protein in colon tissue was significantly decreased(P<0.001),and the expression levels of p-STAT3,NF-κB p65 and VEGF proteins were significantly increased(all P<0.001)in the model group.Compared with the model group,the colon tumor load,colon histopathological score and the levels of p-STAT3,NF-κB p65 and VEGF proteins in colon tissue were significantly decreased(all P<0.05)in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group while the expression levels of P2X7R protein in colon tissue were significantly increased(both P<0.05).Conclusion:Both herb-partitioned moxibustion and ginger-partitioned moxibustion can reduce the colon tumor load in CACC rats and delay the progression of colon adenomas.The mechanism may be mediated by the P2X7R/STAT3 pathway to inhibit STAT3 phosphorylation,thereby reducing VEGF protein expression.