DIA-based proteome profiling with PRM verification reveals the involvement of ER-associated protein processing in pollen abortion in Ogura CMS cabbage

Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.

3-Methyladenine potentiates paclitaxel-induced apoptosis and phosphorylation of cyclin-dependent kinase 1 at thr161 in nasopharyngeal carcinoma cell

Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.

Chinese version of the Perth Alexithymia Questionnaire:psychometric properties and clinical applications

Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.

经营杠杆与企业全要素生产率

本文结合我国供给侧改革及推动经济高质量发展的现实背景,探讨企业杠杆风险的重要来源——经营杠杆是否及如何对企业全要素生产率产生影响。研究发现,经营杠杆的增加会显著抑制企业全要素生产率的提升。拓展性研究表明:(1)增加企业融资约束、降低企业投资效率,是经营杠杆抑制企业全要素生产率提升的重要作用机制;(2)经营杠杆降低企业全要素生产率的作用仅在企业面临更高的经营风险和调整成本时显著,表明企业自身面临较高的经营风险及调整成本水平,是经营杠杆降低企业全要素生产率的重要条件。本文的研究不仅丰富了全要素生产率的影响因素、经营杠杆经济后果的相关文献,对于落实结构化去杠杆,提高企业全要素生产率,实现经济高质量发展,也具有重要启示意义。

基于文献计量学BSA在作物育种领域的应用现状与展望

为了解国内外集群分离分析法(bulked segregant analysis,BSA)在作物育种领域的研究现状与前沿动态,客观反映各国家、机构及研究人员在该领域的影响力,本文基于文献计量学分析方法使用文献计量分析软件CiteSpace对WOS(Web of Science)数据库2000~2023年2111项和CNKI(China National Knowledge Infrastructure)数据库2003~2023年446项研究成果进行关键词共现分析、突显词分析、关键词聚类分析、聚类时间线分析及作者共被引分析。结果表明:BSA在作物育种领域应用的研究成果在国内外的发文趋势相同,国内外期刊发文量均逐年上升;在发文量国家排序中,中国排名第一、美国第二、印度第三。在CNKI数据库中华中农业大学的发文量最多,而在WOS数据库中中国农业科学院的发文量最多。国外BSA在作物育种领域应用的文章主要集中在植物科学、农学、园艺学和遗传学等学科,而国内主要集中在作物学、植物保护学、园艺学、生物学等学科;Michlmore RW、Kosambi DD和Li H这3位作者在该领域的影响力最高,而Michlmore RW、Lander ES、Li H这3位作者与其他作者有更密切的联系。国内研究的热点作物和性状分别是水稻(Oryza sativa)、大豆(Glycine max)、玉米(Zea mays L.)和抗病性、株高;国外研究的热点作物和性状分别是水稻、拟南芥(Arabidopsis thaliana)、小麦(Triticum aestivum L.)和抗病性。目前,BSA在国内集中应用于作物目标性状候选基因及作物突变体突变基因的定位和功能验证,而国外则集中应用于作物目标性状基因的精细定位和功能验证及遗传机制的解析。此外,BSA在作物育种领域应用的研究前沿分析表明,未来在该领域热点研究的对象为水稻、花生(Arachis hypogaea L.)、陆地棉(Gossypium hirsutum L.)、作物突变体和作物代谢产物。

Spatial Downscaling of Remote Sensing Precipitation Data in the Beijing-Tianjin-Hebei Region

Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensing detection technology has become an important source of spatial precipitation data. However, because the spatial resolution of remote sensing precipitation data is still low, it is difficult to meet the needs of hydrological research, which restricts their application in drought and flood analysis, hydrological simulation, etc. In response to this problem, this paper takes the Beijing-Tianjin-Hebei region as the research area, downscaling the TRMM data and the GPM data space of the continuation plan, and increasing the spatial resolution of the data to 1 km. Compared with the original data, spatial downscaling data not only greatly improves the spatial resolution, but also increases the accuracy of the data, which has better applicability.

深度学习在基因组学中的研究进展

随着高通量测序技术的迅猛发展,基因组学领域迎来了数据量的爆炸性增长,这对传统生物信息学处理复杂数据模式的能力构成了严峻挑战。在此技术革新的关键时刻,深度学习作为人工智能领域的前沿技术,以其强大的数据解析与模式识别能力,为基因组学研究注入了新的活力。本文聚焦于4种核心深度学习模型——卷积神经网络(convolution neural network,CNN)、循环神经网络(recurrent neural network,RNN)、长短期记忆网络(long short term memory,LSTM)及生成对抗网络(generative adversarial network,GAN),系统阐述了它们的基础原理,重点回顾了这些模型近5年在DNA、RNA和蛋白质研究领域的广泛应用。此外,文章进一步探讨了深度学习在畜禽基因组学中的应用案例,揭示了其在遗传特征解析、疾病预防以及遗传改良等领域的潜在应用价值与面临的挑战。通过深入分析,本文旨在阐述深度学习技术在增强基因组数据分析的准确性和处理能力方面的作用,并构建一个概念性框架,以指导畜禽基因组学研究策略的发展及其在具体场景下的应用,进而推动精准农业和遗传改良技术的发展。

Effect of nursing intervention on rehabilitation of patients with chronic sinusitis and nasal polyps after nasal endoscopy

BACKGROUND Chronic sinusitis is a kind of chronic suppurative inflammation of the sinus mucosa.Nasal endoscopy is a good method to treat nasal polyps.However postoperative rehabilitation and care should not be neglected.AIM To investigate the Effect of nursing intervention on the rehabilitation of patients with chronic sinusitis and nasal polyps(CSNPS)after nasal endoscopy.METHODS A total of 129 patients with CSNPS hospitalized from February 2017 to February 2019 were studied.Using the digital parity method,we investigated nursing cooperation strategies for endoscopic surgery.The comparison group(64 cases):Surgical nursing was carried out with traditional nursing measures;experimental group(65 cases):Surgical nursing was carried out by traditional nursing countermeasures+comprehensive nursing measures.We compared postoperative recovery rates,nursing satisfaction rates,and nasal cavity ratings between the two groups.RESULTS Experimental group patients with CSNPS had a significantly higher recovery rate(98.46%)compared to the control group(79.69%).This difference was statistically significant(χ2=11.748,P<0.05).Additionally,the satisfaction rate with treatment was also significantly higher in the experimental group(98.46%)compared to the control group(79.69%),with a statistically significant difference(χ2=11.748,P<0.05).Before nursing,there was no significant difference in sinus nasal cavity scores between the experimental group(20.29±7.25 points)and the control group(20.30±7.27 points)(t=0.008,P>0.05).However,after nursing,the sinus nasal cavity score in the experimental group(8.85±3.22 points)was significantly lower than that in the control group(14.99±5.02 points)(t=8.282,P<0.05).CONCLUSION Comprehensive nursing intervention in patients with CSNPS can significantly improve the total recovery rate after endoscopic surgery.

Non-coding RNAs in acute ischemic stroke:from brain to periphery

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

超高龄抑郁症的研究进展

超高龄抑郁症的患病率呈上升趋势,以躯体症状表现为主,易漏诊和误诊,已成为老年人的重要心理健康问题。定期评估情绪状态,有助于为超高龄老人及时制定干预措施。综述了超高龄抑郁症的临床研究进展,对这类患者宜及时采用适当心理治疗和抗抑郁药治疗,期望为超高龄老年抑郁症的诊疗提供参考。

TRPC6 Knockout Alleviates Renal Fibrosis through PI3K/AKT/GSK3B Pathway

Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage.Notably,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal fibrosis.However,the regulatory impact of TRPC6 on renal fibrosis remains unclear.Methods In vivo,TRPC6 knockout(TRPC6−/−)mice and age-matched 129 SvEv(WT)mice underwent unilateral renal ischemia-reperfusion(uIR)injury surgery on the left renal pedicle or sham operation.Kidneys and serum were collected on days 7,14,21,and 28 after euthanasia.In vitro,primary tubular epithelial cells(PTECs)were isolated from TRPC6−/−and WT mice,followed by treatment with transforming growth factorβ1(TGFβ1)for 72 h.The anti-fibrotic effect of TRPC6−/−and the underlying mechanisms were assessed through hematoxylin-eosin staining,Masson staining,immunostaining,qRT-PCR,and Western blotting.Results Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFβ1.TRPC6−/−alleviated renal fibrosis by reducing the expression of fibrotic markers(Col-1,α-SMA,and vimentin),as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in vitro.Additionally,we found that the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK3β)signaling pathway,a pivotal player in renal fibrosis,was down-regulated following TRPC6 deletion.Conclusion These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3βpathway.Targeting TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.

Umbilical cord mesenchymal stem cell exosomes alleviate necrotizing enterocolitis in neonatal mice by regulating intestinal epithelial cells autophagy

BACKGROUND Necrotizing enterocolitis(NEC)is a severe gastrointestinal disease that affects premature infants.Although mounting evidence supports the therapeutic effect of exosomes on NEC,the underlying mechanisms remain unclear.AIM To investigate the mechanisms underlying the regulation of inflammatory response and intestinal barrier function by umbilical cord mesenchymal stem cell(UCMSCs)exosomes,as well as their potential in alleviating NEC in neonatal mice.METHODS NEC was induced in 5-d-old C57BL/6 pups through hypoxia and gavage feeding of formula containing lipopolysaccharide(LPS),after which the mice received human UCMSC exosomes(hUCMSC-exos).The control mice were allowed to breastfeed with their dams.Ileal tissues were collected from the mice and analyzed by histopathology and immunoblotting.Colon tissues were collected from NEC neonates and analyzed by immunofluorescence.Molecular biology and cell culture approaches were employed to study the related mechanisms in intestinal epithelial cells.RESULTS We found that autophagy is overactivated in intestinal epithelial cells during NEC,resulting in reduced expression of tight junction proteins and an increased inflammatory response.The ability of hUCMSC-exos to ameliorate NEC in a mouse model was dependent on decreased intestinal autophagy.We also showed that hUCMSC-exos alleviate the inflammatory response and increase migration ability in intestinal epithelial cells induced by LPS.CONCLUSION These results contribute to a better understanding of the protective mechanisms of hUCMSC-exos against NEC and provide a new theoretical and experimental foundation for NEC treatment.These findings also enhance our understanding of the role of the autophagy mechanism in NEC,offering potential avenues for identifying new therapeutic targets.

Hybrid Multi-Infeed Interaction Factor Calculation Method Considering Voltage Regulation Control Characteristics of Voltage Source Converter

Voltage source converter based high voltage direct current(VSC-HVDC)can participate in voltage regulation by flexible control to help maintain the voltage stability of the power grid.In order to quantitatively evaluate its influence on the voltage interaction between VSC-HVDC and line commutated converter based high voltage direct current(LCC-HVDC),this paper proposes a hybrid multi-infeed interaction factor(HMIIF)calculation method considering the voltage regulation control characteristics of VSC-HVDC.Firstly,for a hybrid multi-infeed high voltage direct current system,an additional equivalent operating admittance matrix is constructed to characterize HVDC equipment characteristics under small disturbance.Secondly,based on the characteristic curve between the reactive power and the voltage of a certain VSC-HVDC project,the additional equivalent operating admittance of VSC-HVDC is derived.The additional equivalent operating admittance matrix calculation method is proposed.Thirdly,the equivalent bus impedance matrix is obtained by modifying the alternating current(AC)system admittance matrix with the additional equivalent operating admittance matrix.On this basis,the HMIIF calculation method based on the equivalent bus impedance ratio is proposed.Finally,the effectiveness of the proposed method is verified in a hybrid dual-infeed high voltage direct current system constructed in Power Systems Computer Aided Design(PSCAD),and the influence of voltage regulation control on HMIIF is analyzed.

穴位按压对老年转移性结直肠癌维持治疗患者癌因性疲乏的干预效果

目的:观察穴位按压对老年转移性结直肠癌维持治疗患者癌因性疲乏(cancer related fatigue,CRF)的干预效果。方法:前瞻性收集98例存在CRF的老年(≥65岁)转移性结直肠癌维持治疗患者,参照随机数字表法随机分为观察组50例和对照组48例。对照组仅接受常规健康指导,观察组在此基础上给予穴位按压,双侧取穴合谷、三阴交、足三里,按压手法经研究者培训指导后,由患者自行操作,每次每侧每个穴位持续按压5 min,共30 min,每天3次。两组均连续干预6周。干预前后,采用Piper疲乏修订量表和匹兹堡睡眠质量指数量表评估患者疲乏和睡眠质量变化情况,检测外周血T淋巴细胞亚群指标。结果:干预后,观察组Piper疲乏修订量表和匹兹堡睡眠质量指数量表评分低于对照组,观察组外周血CD3^(+)、CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平高于对照组,差异有统计学意义(P<0.05)。结论:穴位按压有助于减轻老年转移性结直肠癌维持治疗患者CRF,提高睡眠质量,可能与改善患者自身免疫功能有关。

Xuefu Zhuyu decoction improves neurological dysfunction by increasing synapsin expression after traumatic brain injury

Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic brain injury by controlled cortical impact. Rat models were intragastrically administered 9 and 18 g/kg Xuefu Zhuyu decoction once a day for 14 or 21 days. Changes in neurological function were assessed by modified neurological severity scores and the Morris water maze. Immunohistochemistry, western blot assay, and re- verse-transcription polymerase chain reaction were used to analyze synapsin protein and mRNA expression at the injury site of rats. Our results showed that Xuefu Zhuyu decoction visibly improved neurological function of rats with traumatic brain injury. These changes were accompanied by increased expression of synaptophysin, synapsin I, and postsynaptic density protein-95 protein and mRNA in a dose-de- pendent manner. These findings indicate that Xuefu Zhuyu decoction increases synapsin expression and improves neurological deficits alder traumatic brain injury.

Short Hairpin RNA-mediated MDR1 Gene Silencing Increases Apoptosis of Human Ovarian Cancer Cell Line A2780/Taxol

Objective： Recurrent ovarian cancer is often resistant to drugs such as paclitaxel. Short hairpin RNA （shRNA） targeting MDRI, a gene involved in the process of drug resistance, may be a promising strategy to overcome drug resistance. Methods： Construction and identification of eukaryotic expression plasmid was transiently transfected into human ovarian cancer ce plasmid of shRNA targeting on MDR1 gene. The ne A2780/Taxol. Apoptosis was determined by flow cytometry using annexin V-FITC/PI double labeling. Expression of MDRI mRNA was detected by quantitative polymerase chain reaction （qPCR） and P-glycoprotein expression was detected using Western blot. Results The IC50 of paclitaxel in MDR1 shRNA-transfected group was significantly reduced （1.986±0.153）μmol/ml as compared with that in negative control （5.246±0.107）μmol/ml and empty vector-transfected group （5.212±0.075）μmol/ml （P〈0.05）. The percent of the relative reverse sensitivity to paclitaxel on A2780/Taxol cells was 67.1%, and the apoptotic rate was significantly increased [（6.977±0.333）%] compared with control [（1.637±0.111）%] and empty vector-transfected group [（1.663±0.114）%] （P〈0.05）. Expressions of MDR1 mRNA and P-glycoprotein were significantly reduced compared with control （P〈0.05）. Conclusion： The present study demonstrated that the eukaryotic expression plasmid of shRNA targeting on MDRI inhibited the expression of MDRI effectively, thus enhance the sensitivity of A2780/Taxol cells to paclitaxel.

一种快速固化耐酸防腐酚醛环氧涂料的研制

针对储存酸性介质的石油化工储罐及压力容器的防腐需求,采用F-51、F-53酚醛环氧树脂,Ancamine 2753脂环胺和NX-2003D腰果壳油改性酚醛胺固化剂,添加叔胺固化促进剂,辅以0.8%的石墨烯分散体、5%的苯甲醇增塑剂等原料,研制出一款耐酸性介质性能优异的快速固化防腐涂料。它附着力高,常温下固化只需4 d,适用于油田与化工储罐及压力容器的现场防腐维修。

Perinipple Broken Line Incision: a Novel Approach for Breast Augmentation

Objective To investigate reliability of the infra-nipple broken line incision for breast augmentation. Methods From January 2012 to January 2013, 15 patients underwent primary bilateral retromuscular breast augmentation with round textured silicone-gel implants and a novel infra-nipple broken line incision. Preoperatively, a semicircular incision was marked along the inferior base of the nipple. It was then extended bilaterally using two transverse right-angled geometric broken lines within the pigmented areolar skin. Follow-up was performed to evaluate the sensation of nipple-areolar complex, the scar, and the shape and texture of the breasts. Results The average follow-up was 6.7 months. Most of the patients complained of paresthesia of the nipple or breast skin, but transient decreased sensation improved within 3 months. No patients showed permanent sensory changes of the nipple areolar complex at a minimum follow-up of 4 months. The scars were imperceptible in all patients. Conclusion We believe that for selected patients, the infra-nipple broken line incision is a practical and reliable method to achieve aesthetic result.

Numerical Characterization of the Annular Flow Behavior and Pressure Loss in Deepwater Drilling Riser

In drilling a deepwater well,the mud density window is narrow,which needs a precise pressure control to drill the well to its designed depth.Therefore,an accurate characterization of annular flow between the drilling riser and drilling string is critical in well control and drilling safety.Many other factors influencing the change of drilling pressure that should be but have not been studied sufficiently.We used numerical method to simulate the process of drill string rotation and vibration in the riser to show that the rotation and transverse vibration of drill string can increase the axial velocity in the annulus,which results in the improvement of the flow field in the annulus,and the effect on pressure loss and its fluctuation amplitude.In addition,there are also multiple secondary flow vortices in the riser annulus under certain eccentricity conditions,which is different from the phenomenon in an ordinary wellbore.The findings of this research are critical in safely controlling well drilling operation in the deepwater environment.

New Approach to Chinese Writing:an Exploratory Study of Writing Performance on Social Q&A Online Community

Many studies have investigated how the online community has informed language acquisition and teaching.In the scholarship of Computer-Mediated Communication Theory(CMC),while most of the studies have emphasized second language English writing using the CMC framework,evidence is shown to support CMC in foreign language instruction.However,there is a lack of research on how feedback from native speakers of Chinese influences Chinese learners'writing in a web environment.In response to the research gap,drawing on sociocultural and CMC,the current exploratory study probes the nature of feedback on Zhihu,the largest Chinese social question-and-answer website,and how feedback affects advanced Chinese learners'writing performance.Eight American university students learning Chinese as second language participated by completing two writing tasks on Zhihu.After posting essays on the website,these participants read and responded to online feedback within two weeks,followed by a revision of their original posts.Tokens of feedback,revised language items and learners'response to feedback were coded by the researcher.Findings reported feedback types from Zhihu users,exchanges between learners and feedback providers,and correlations between first and second drafts.Feedback on Zhihu is proven to be beneficial to advanced Chinese learners'writing performance,and pedagogical implication is suggested for the inclusion of Zhihu in advanced Chinese curriculum.