目的:观察隔药饼灸对高脂血症合并动脉粥样硬化兔脂联素(A D P N)、基质金属蛋白酶-3(MMP-3)的影响。方法:取新西兰兔32只,随机分为正常组、模型组、隔药饼灸组、阿托伐他汀组,每组8只。给予高胆固醇饲料喂养以制作高脂血症模型。各组...目的:观察隔药饼灸对高脂血症合并动脉粥样硬化兔脂联素(A D P N)、基质金属蛋白酶-3(MMP-3)的影响。方法:取新西兰兔32只,随机分为正常组、模型组、隔药饼灸组、阿托伐他汀组,每组8只。给予高胆固醇饲料喂养以制作高脂血症模型。各组予相应方法干预。HE染色比较各组兔腹主动脉管壁结构,检测血脂[低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)]、肿瘤坏死因子-α(TNF-α)、ADPN、MMP-3含量。结果:正常组主动脉壁结构各层清晰,模型组内膜增厚,泡沫细胞聚集,两治疗组内膜稍增厚,内壁较平整。与正常组比较,模型组兔血清LDL-C、TG、TC、TNF-α、MMP-3水平均显著升高(P<0.01),ADPN水平显著下降(P<0.05);与模型组比较,两治疗组血清LDL-C、TG、TC、TNF-α水平均显著下降(P<0.05,P<0.01),ADPN水平均显著上升(P<0.05),隔药饼灸组血清MMP-3水平显著下降(P<0.01)。血清LDL-C、TG、TC、TNF-α水平与MMP-3含量呈正相关,与ADPN含量呈负相关(P<0.01,P<0.05)。结论:隔药饼灸可通过调节MMP-3、ADPN水平,抑制炎症反应,对动脉粥样硬化产生保护作用,且MMP-3、ADPN水平与血脂相关指标有明显相关性,可作为评价高脂血症疗效的新型指标。展开更多
Objective:To observe the clinical efficacy of treating infantile diarrhea due to spleen deficiency with five-step pediatric tuina of Huxiang school.Methods:Using a randomized controlled trial design,sixty eligible kid...Objective:To observe the clinical efficacy of treating infantile diarrhea due to spleen deficiency with five-step pediatric tuina of Huxiang school.Methods:Using a randomized controlled trial design,sixty eligible kids with diarrhea due to spleen deficiency were randomized into an observation group and a control group,with 30 cases in each group.The observation group was intervened by the five-step pediatric tuina method of Huxiang school,and the control group received conventional tuina treatment.The intervention was conducted once a day,consecutive 5-day treatment as 1 course,at a 2-day interval between courses,successively for a total of 4 courses.Changes in the primary and secondary symptoms of diarrhea due to spleen deficiency were observed,and the clinical efficacy was evaluated.Results:After treatment,the scores of primary and secondary symptoms and the general score of diarrhea due to spleen deficiency were improved;the improvements in fecal form and frequency,decreased appetite,bloating after meals and fatigue and sluggishness were more significant in the observation group than in the control group.Conclusion:The five-step pediatric tuina method of Huxiang school and conventional tuina both can improve the primary and secondary symptoms in infantile diarrhea due to spleen deficiency,while the former one can produce more significant efficacy.展开更多
Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its targe...Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues.Methods:Sixty Sprague-Dawley rats were divided into a blank control group,a sham operation group,a model group,an acupuncture group,a mild hypothermia group and an acup un cture plus mild hypothermia group accordi ng to the random nu mbertable method(n=10).Except for the blank c on trol group an dthe sham operati on group,rats in the other 4 groups received CIRI modeling.After the model was successfully established,rats in the blank control group were bred routinely for 72 h without any interventions;rats in the sham operation group and the model group were bred routinely for 72 h,and only received bin ding without other interve ntions after surgery;rats in the acup un cture group were bred routinely for 72 h,and received acupuncture at Dazhui(GV 14),Baihui(GV 20)and Shuigou(GV 26)after binding;rats in the mild hypothermia group were bred routinely for 72 h,and received mild hypothermia intervention for 72 h after binding;rats in the acupuncture plus mild hypothermia group were bred routinely for 72 h,followed by receiving acupuncture as in the acup uncture group and mild hypothermia therapy as in the mild hypothermia group after bin ding.The n eurological impairme nt score,cerebral infarcti on area ratio,the expressions of miRNA-204 and its target genes in eluding Map3k8,Ntrk2 and Ppp3rl in the ischemic hippocampus of each group were observed after 72 h of intervention.Results:Before intervention,compared with the bgnk control group and the sham operation group,the neurological impairment scores and the infarction area ratios of the modelled rats were statistically significa ntly increased(all P<Q.Ql)f indicating that the model was successful.After intervention,compared with the model group,the neurological impairment scores of the three in tervention groups were sign ifica ntly reduced(all P<0.01);compared with the acupuncture group and the mild hypothermia group,the infarction area ratio in the acupuncture plus mild hypothermia group was signtiicantly reduced(both P<0.01);compared with the model group,the three intervention groups showed significant inhibition of miRNA-204 expression in brain tissues(all P<0.05),which was most significant in the acupuncture plus mild hypothermia group(P<0.01);compared with the acupuncture group and the mild hypothermia group,the Map3k8 expression in the acupuncture plus mild hypothermia group was significantly increased(both P<0.01),but there were no sign ificant d iff ere nces in Ntrk2 and Ppp3rl expressions between groups(all P>0.05).Conclusion:Acupuncture,mild hypothermia,and acupuncture plus mild hypothermia reduced the neurological impairment score and the cerebral infarction area in CIRI rats,while acupuncture plus mild hypothermia showed the most significant effect.In regulating miRNA-204 target gene expressions,acupuncture plus mild hypothermia showed the same effect on Ntrk2 and Ppp3rl expressions,while better effect on Map3k8 expression compared with either acupuncture or hypothermia.展开更多
Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no...Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no rmal group, a model group, a herbal cake-partitioned moxibustion group and a simvastatin group according to the random number table method, with 9 rabbits in each group. Rabbits in the model group, the herbal cake-partitioned moxibustion group and the simvastatin group were modeled by high fat feeding method which took 12 weeks. After verification of the successful model, rabbits in the normal group were not treated, in the model group were bun died, in the herbal cake-partitioned moxibustio n group were treated with herbal cake-partitioned moxibustion, and those in the simvastatin group were treated with simvastatin, all for a total of 4 weeks. At the end of the experiment, the aorta and liver were observed for pathological changes;serum and liver were used to detect lipid levels;Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect LXRa protein and mRNA expression levels, respectively. Results: Compared with the normal group, the structure of aorta was disordered, the wall was rough and thick, the intima was unsmooth, and the vascular smooth muscle cells were arranged closely and disorderly in the model group, which was consistent with the characteristics of the rabbit model of aortic atherosclerosis. Compared with the model group, the aortic structure was clear;the degree of hepatocyte degeneration was reduced, the serum total cholesterol and low-density lipoprotein levels were significantly decreased (all P<0.01), the high-density lipoprotein level was elevated (P<0.01), and the total liver cholesterol was decreased significantly (P<0.01) in the rabbits of the herbal cake-partitioned moxibustion group and the simvastatin group;compared with the model group, the protein (PvO.Ol or P<0.05) and mRNA (P<0.01) expressions of rabbit LXRa in the herbal cake-partiti oned moxibusti on group and the simvastati n group were in creased. Conclusion: Herbal cake-partitioned moxibustion can improve the aortic and hepatic lesions, regulate blood lipid and liver lipid levels, in crease the expressi on of liver cholesterol reverse tran sport nu clear receptor LXRa, promote reverse cholesterol transport in the rabbits with aortic atherosclerosis, therefore produces an antiatherogenic effect.展开更多
Objective:To observe the difference in clinical efficacy between acupuncture with point selection based on syndrome differentiation along the meridians and acupuncture at non-meridian and non-acupoint points for funct...Objective:To observe the difference in clinical efficacy between acupuncture with point selection based on syndrome differentiation along the meridians and acupuncture at non-meridian and non-acupoint points for functional dyspepsia(FD).Methods:A total of 74 FD patients were randomized into an observation group and a control group,with 37 cases in each group.Both groups received acupuncture treatment.Zusanli(ST 36)and Neiguan(PC 6)were selected in the observation group,with Taichong(LR 3)and Neiting(ST 44)added for excess syndrome,and Gongsun(SP 4)and Yinlingquan(SP 9)added for deficiency syndrome.Four non-meridian and non-acupoint points were selected in the control group.The treatments in both groups were performed once a day with a 2-day break after 5 consecutive treatments,which constituted one treatment course.A total of 4 courses were performed.The scores of Nepean dyspepsia index(NDI)and Leeds dyspepsia questionnaire(LDQ)were recorded before and after treatment,and during follow-up(8,12,16,20 and 24 weeks after recruitment)to assess the clinical efficacy.Results:The NDI scores in the two groups after treatment and at each time point during follow-up were higher than those before treatment(all P<0.05),and the LDQ scores were lower than those before treatment(all P<0.05).The NDI scores after treatment and at each time point during follow-up in the observation group were higher than those in the control group(all P<0.01);the total LDQ score and scores of upper abdominal pain,postprandial satiety and upper abdominal burning sensation after treatment and at each time point during follow-up in the observation group were significantly lower than those in the control group(P<0.01 or P<0.05)..Conclusion:Acupuncture with point selection based on syndrome differentiation along the meridians has a better curative effect than acupuncture at non meridian and non-acupoint points in the treatment of FD.展开更多
The suspended magnetic moxibustion cupping therapy, as a specific moxibustion method which collects moxibustion, cupping, infrared and magnetic therapy into one, and integrates with the warming and heating effect of m...The suspended magnetic moxibustion cupping therapy, as a specific moxibustion method which collects moxibustion, cupping, infrared and magnetic therapy into one, and integrates with the warming and heating effect of moxibustion, sucking effect of cups, and effect of infrared light energy and magnetic energy, is a particularly unique method for weight loss. This article introduces the basic situation and mechanism of suspended magnetic cupping therapy for weight loss. For pathogenesis of deficiency, phlegm, stagnation and cold in obesity, Shuang Long Xi Zhu （two dragons playing a ball） moxibustion, abdominal Jiu Gong （nine palaces） moxibustion, San Yang Kai Tai （auspicious beginning） moxibustion and Chang Long （long dragon） moxibustion were given respectively. This therapy has inspired the clinical idea of Chinese medicine for obesity.展开更多
Telehealth was defined as the use of information, computers and telecommunication technologies to provide health related services when health professionals and patients are separated by a distance This new medical mod...Telehealth was defined as the use of information, computers and telecommunication technologies to provide health related services when health professionals and patients are separated by a distance This new medical model involves video-conference, transmission of images, e-health portals, remote monitoring of vital signs, and continuing medical education as a whole to help patients to improve health care. It should consist of three parts, including the patient client, the specialist and the connector （platform and intemet）.展开更多
文摘目的:观察隔药饼灸对高脂血症合并动脉粥样硬化兔脂联素(A D P N)、基质金属蛋白酶-3(MMP-3)的影响。方法:取新西兰兔32只,随机分为正常组、模型组、隔药饼灸组、阿托伐他汀组,每组8只。给予高胆固醇饲料喂养以制作高脂血症模型。各组予相应方法干预。HE染色比较各组兔腹主动脉管壁结构,检测血脂[低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)]、肿瘤坏死因子-α(TNF-α)、ADPN、MMP-3含量。结果:正常组主动脉壁结构各层清晰,模型组内膜增厚,泡沫细胞聚集,两治疗组内膜稍增厚,内壁较平整。与正常组比较,模型组兔血清LDL-C、TG、TC、TNF-α、MMP-3水平均显著升高(P<0.01),ADPN水平显著下降(P<0.05);与模型组比较,两治疗组血清LDL-C、TG、TC、TNF-α水平均显著下降(P<0.05,P<0.01),ADPN水平均显著上升(P<0.05),隔药饼灸组血清MMP-3水平显著下降(P<0.01)。血清LDL-C、TG、TC、TNF-α水平与MMP-3含量呈正相关,与ADPN含量呈负相关(P<0.01,P<0.05)。结论:隔药饼灸可通过调节MMP-3、ADPN水平,抑制炎症反应,对动脉粥样硬化产生保护作用,且MMP-3、ADPN水平与血脂相关指标有明显相关性,可作为评价高脂血症疗效的新型指标。
文摘Objective:To observe the clinical efficacy of treating infantile diarrhea due to spleen deficiency with five-step pediatric tuina of Huxiang school.Methods:Using a randomized controlled trial design,sixty eligible kids with diarrhea due to spleen deficiency were randomized into an observation group and a control group,with 30 cases in each group.The observation group was intervened by the five-step pediatric tuina method of Huxiang school,and the control group received conventional tuina treatment.The intervention was conducted once a day,consecutive 5-day treatment as 1 course,at a 2-day interval between courses,successively for a total of 4 courses.Changes in the primary and secondary symptoms of diarrhea due to spleen deficiency were observed,and the clinical efficacy was evaluated.Results:After treatment,the scores of primary and secondary symptoms and the general score of diarrhea due to spleen deficiency were improved;the improvements in fecal form and frequency,decreased appetite,bloating after meals and fatigue and sluggishness were more significant in the observation group than in the control group.Conclusion:The five-step pediatric tuina method of Huxiang school and conventional tuina both can improve the primary and secondary symptoms in infantile diarrhea due to spleen deficiency,while the former one can produce more significant efficacy.
文摘Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues.Methods:Sixty Sprague-Dawley rats were divided into a blank control group,a sham operation group,a model group,an acupuncture group,a mild hypothermia group and an acup un cture plus mild hypothermia group accordi ng to the random nu mbertable method(n=10).Except for the blank c on trol group an dthe sham operati on group,rats in the other 4 groups received CIRI modeling.After the model was successfully established,rats in the blank control group were bred routinely for 72 h without any interventions;rats in the sham operation group and the model group were bred routinely for 72 h,and only received bin ding without other interve ntions after surgery;rats in the acup un cture group were bred routinely for 72 h,and received acupuncture at Dazhui(GV 14),Baihui(GV 20)and Shuigou(GV 26)after binding;rats in the mild hypothermia group were bred routinely for 72 h,and received mild hypothermia intervention for 72 h after binding;rats in the acupuncture plus mild hypothermia group were bred routinely for 72 h,followed by receiving acupuncture as in the acup uncture group and mild hypothermia therapy as in the mild hypothermia group after bin ding.The n eurological impairme nt score,cerebral infarcti on area ratio,the expressions of miRNA-204 and its target genes in eluding Map3k8,Ntrk2 and Ppp3rl in the ischemic hippocampus of each group were observed after 72 h of intervention.Results:Before intervention,compared with the bgnk control group and the sham operation group,the neurological impairment scores and the infarction area ratios of the modelled rats were statistically significa ntly increased(all P<Q.Ql)f indicating that the model was successful.After intervention,compared with the model group,the neurological impairment scores of the three in tervention groups were sign ifica ntly reduced(all P<0.01);compared with the acupuncture group and the mild hypothermia group,the infarction area ratio in the acupuncture plus mild hypothermia group was signtiicantly reduced(both P<0.01);compared with the model group,the three intervention groups showed significant inhibition of miRNA-204 expression in brain tissues(all P<0.05),which was most significant in the acupuncture plus mild hypothermia group(P<0.01);compared with the acupuncture group and the mild hypothermia group,the Map3k8 expression in the acupuncture plus mild hypothermia group was significantly increased(both P<0.01),but there were no sign ificant d iff ere nces in Ntrk2 and Ppp3rl expressions between groups(all P>0.05).Conclusion:Acupuncture,mild hypothermia,and acupuncture plus mild hypothermia reduced the neurological impairment score and the cerebral infarction area in CIRI rats,while acupuncture plus mild hypothermia showed the most significant effect.In regulating miRNA-204 target gene expressions,acupuncture plus mild hypothermia showed the same effect on Ntrk2 and Ppp3rl expressions,while better effect on Map3k8 expression compared with either acupuncture or hypothermia.
文摘Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no rmal group, a model group, a herbal cake-partitioned moxibustion group and a simvastatin group according to the random number table method, with 9 rabbits in each group. Rabbits in the model group, the herbal cake-partitioned moxibustion group and the simvastatin group were modeled by high fat feeding method which took 12 weeks. After verification of the successful model, rabbits in the normal group were not treated, in the model group were bun died, in the herbal cake-partitioned moxibustio n group were treated with herbal cake-partitioned moxibustion, and those in the simvastatin group were treated with simvastatin, all for a total of 4 weeks. At the end of the experiment, the aorta and liver were observed for pathological changes;serum and liver were used to detect lipid levels;Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect LXRa protein and mRNA expression levels, respectively. Results: Compared with the normal group, the structure of aorta was disordered, the wall was rough and thick, the intima was unsmooth, and the vascular smooth muscle cells were arranged closely and disorderly in the model group, which was consistent with the characteristics of the rabbit model of aortic atherosclerosis. Compared with the model group, the aortic structure was clear;the degree of hepatocyte degeneration was reduced, the serum total cholesterol and low-density lipoprotein levels were significantly decreased (all P<0.01), the high-density lipoprotein level was elevated (P<0.01), and the total liver cholesterol was decreased significantly (P<0.01) in the rabbits of the herbal cake-partitioned moxibustion group and the simvastatin group;compared with the model group, the protein (PvO.Ol or P<0.05) and mRNA (P<0.01) expressions of rabbit LXRa in the herbal cake-partiti oned moxibusti on group and the simvastati n group were in creased. Conclusion: Herbal cake-partitioned moxibustion can improve the aortic and hepatic lesions, regulate blood lipid and liver lipid levels, in crease the expressi on of liver cholesterol reverse tran sport nu clear receptor LXRa, promote reverse cholesterol transport in the rabbits with aortic atherosclerosis, therefore produces an antiatherogenic effect.
文摘Objective:To observe the difference in clinical efficacy between acupuncture with point selection based on syndrome differentiation along the meridians and acupuncture at non-meridian and non-acupoint points for functional dyspepsia(FD).Methods:A total of 74 FD patients were randomized into an observation group and a control group,with 37 cases in each group.Both groups received acupuncture treatment.Zusanli(ST 36)and Neiguan(PC 6)were selected in the observation group,with Taichong(LR 3)and Neiting(ST 44)added for excess syndrome,and Gongsun(SP 4)and Yinlingquan(SP 9)added for deficiency syndrome.Four non-meridian and non-acupoint points were selected in the control group.The treatments in both groups were performed once a day with a 2-day break after 5 consecutive treatments,which constituted one treatment course.A total of 4 courses were performed.The scores of Nepean dyspepsia index(NDI)and Leeds dyspepsia questionnaire(LDQ)were recorded before and after treatment,and during follow-up(8,12,16,20 and 24 weeks after recruitment)to assess the clinical efficacy.Results:The NDI scores in the two groups after treatment and at each time point during follow-up were higher than those before treatment(all P<0.05),and the LDQ scores were lower than those before treatment(all P<0.05).The NDI scores after treatment and at each time point during follow-up in the observation group were higher than those in the control group(all P<0.01);the total LDQ score and scores of upper abdominal pain,postprandial satiety and upper abdominal burning sensation after treatment and at each time point during follow-up in the observation group were significantly lower than those in the control group(P<0.01 or P<0.05)..Conclusion:Acupuncture with point selection based on syndrome differentiation along the meridians has a better curative effect than acupuncture at non meridian and non-acupoint points in the treatment of FD.
文摘The suspended magnetic moxibustion cupping therapy, as a specific moxibustion method which collects moxibustion, cupping, infrared and magnetic therapy into one, and integrates with the warming and heating effect of moxibustion, sucking effect of cups, and effect of infrared light energy and magnetic energy, is a particularly unique method for weight loss. This article introduces the basic situation and mechanism of suspended magnetic cupping therapy for weight loss. For pathogenesis of deficiency, phlegm, stagnation and cold in obesity, Shuang Long Xi Zhu （two dragons playing a ball） moxibustion, abdominal Jiu Gong （nine palaces） moxibustion, San Yang Kai Tai （auspicious beginning） moxibustion and Chang Long （long dragon） moxibustion were given respectively. This therapy has inspired the clinical idea of Chinese medicine for obesity.
文摘Telehealth was defined as the use of information, computers and telecommunication technologies to provide health related services when health professionals and patients are separated by a distance This new medical model involves video-conference, transmission of images, e-health portals, remote monitoring of vital signs, and continuing medical education as a whole to help patients to improve health care. It should consist of three parts, including the patient client, the specialist and the connector （platform and intemet）.
