为了提升信道状态信息(channel state information,CSI)指纹室内定位的性能,提出了一种改进MixNet的CSI图像指纹室内定位方法.在离线阶段,通过选择定位参考点(reference point,RP)处信号强度指示(received signal strength indication,R...为了提升信道状态信息(channel state information,CSI)指纹室内定位的性能,提出了一种改进MixNet的CSI图像指纹室内定位方法.在离线阶段,通过选择定位参考点(reference point,RP)处信号强度指示(received signal strength indication,RSSI)最强的3个接入点(access point,AP),提取其CSI数据并转换为图像;然后利用改进的MixNet模型对图像进行训练并保存模型.其中改进的MixNet引入了坐标注意力(coordinate attention,CA)和残差连接.首先,将MixNet-s中的SE(squeeze-and-excitation)注意力替换为CA,以增强网络的信息表示能力并更精确地获取CSI图像指纹特征.其次,根据MixNet-s模型的特点构建残差连接,以增强网络的表示能力并防止过拟合.最后,通过减小网络深度确保所有网络层得到充分训练;在线阶段,采集目标设备的CSI数据并转换为图像,输入已训练好的改进MixNet模型(命名为MixNet-CA);最后利用加权质心算法根据模型输出的概率值估计目标设备的最终位置.提出方法在室内环境中进行了验证,达到了0.3620 m的平均定位误差.展开更多
Ginsenoside Rg5 is a rare ginsenoside showing promising tumor-suppressive effects.This study aimed to explore its radio-sensitizing effects and the underlying mechanisms.Human lung adenocarcinoma cell lines A549 and C...Ginsenoside Rg5 is a rare ginsenoside showing promising tumor-suppressive effects.This study aimed to explore its radio-sensitizing effects and the underlying mechanisms.Human lung adenocarcinoma cell lines A549 and Calu-3 were used for in vitro and in vivo analysis.Bioinformatic molecular docking prediction and following validation by surface plasmon resonance(SPR)technology,cellular thermal shift assay(CETSA),and isothermal titration calorimetry(ITC)were conducted to explore the binding between ginsenoside Rg5 and 90 kD heat shock protein alpha(HSP90a).The effects of ginsenoside Rg5 on HSP90-cell division cycle 37(CDC37)interaction,the client protein stability,and the downstream regulations were further explored.Results showed that ginsenoside Rg5 could induce cell-cycle arrest at the G1 phase and enhance irradiationinduced cell apoptosis.It could bind to HSP90a with a high affinity,but the affinity was drastically decreased by HSP90a Y61A mutation.Co-immunoprecipitation(Co-IP)and ITC assays confirmed that ginsenoside Rg5 disrupts the HSP90-CDC37 interaction in a dose-dependent manner.It reduced irradiation-induced upregulation of the HSP90-CDC37 client proteins,including SRC,CDK4,RAF1,and ULK1 in A549 cell-derived xenograft(CDX)tumors.Ginsenoside Rg5 or MRT67307(an IKKε/TBK1 inhibitor)pretreatment suppressed irradiation-induced elevation of the LC3-II/b ratio and restored irradiation-induced downregulation of p62 expression.In A549 CDX tumors,ginsenoside Rg5 treatment suppressed LC3 expression and enhanced irradiation-induced DNA damage.In conclusion,ginsenoside Rg5 may be a potential radiosensitizer for lung adenocarcinoma.It interacts with HSP90a and reduces the binding between HSP90 and CDC37,thereby increasing the ubiquitin-mediated proteasomal degradation of the HSP90-CDC37 client proteins.展开更多
This study aims to realize the sharing of near-infrared analysis models of lignin and holocellulose content in pulp wood on two different batches of spectrometers and proposes a combined algorithm of SPA-DS,MCUVE-DS a...This study aims to realize the sharing of near-infrared analysis models of lignin and holocellulose content in pulp wood on two different batches of spectrometers and proposes a combined algorithm of SPA-DS,MCUVE-DS and SiPLS-DS.The Successive Projection Algorithm(SPA),the Monte-Carlo of Uninformative Variable Elimination(MCUVE)and the Synergy Interval Partial Least Squares(SiPLS)algorithms are respectively used to reduce the adverse effects of redundant information in the transmission process of the full spectrum DS algorithm model.These three algorithms can improve model transfer accuracy and efficiency and reduce the manpower and material consumption required for modeling.These results show that the modeling effects of the characteristic wavelengths screened by the SPA,MCUVE and SiPLS algorithms are all greatly improved compared with the full-spectrum modeling,in which the SPA-PLS result in the best prediction with RPDs above 6.5 for both components.The three wavelength selection methods combined with the DS algorithm are used to transfer the models of the two instruments.Among them,the MCUVE combined with the DS algorithm has the best transfer effect.After the model transfer,the RMSEP of lignin is 0.701,and the RMSEP of holocellulose is 0.839,which was improved significantly than the full-spectrum model transfer of 0.759 and 0.918.展开更多
对于温度灵敏度优于10 mK的长波红外相机,如何精确测量表征其灵敏度的指标-噪声等效温差(Noise Equivalent Temperature Difference,NETD),是一项重要的工作。首先,依照尽量缩短标准辐射传递链路减少误差项的原则,对比不同的测量方法,选...对于温度灵敏度优于10 mK的长波红外相机,如何精确测量表征其灵敏度的指标-噪声等效温差(Noise Equivalent Temperature Difference,NETD),是一项重要的工作。首先,依照尽量缩短标准辐射传递链路减少误差项的原则,对比不同的测量方法,选取"黑体直接测量法";其次,基于相机噪声理论模型,对不同温度点下的实测噪声分析,提出噪声修正方法对噪声进行修正,以此减小环境影响带来的测量误差;最后,基于NETD测量计算公式及不确定度分析理论对测试结果的不确定度进行分析评定。结果表明:针对甚高灵敏度长波红外相机所采用的测试设备及方法,可满足测试要求,测试结果相对不确定度在7.7%左右。展开更多
文摘为了提升信道状态信息(channel state information,CSI)指纹室内定位的性能,提出了一种改进MixNet的CSI图像指纹室内定位方法.在离线阶段,通过选择定位参考点(reference point,RP)处信号强度指示(received signal strength indication,RSSI)最强的3个接入点(access point,AP),提取其CSI数据并转换为图像;然后利用改进的MixNet模型对图像进行训练并保存模型.其中改进的MixNet引入了坐标注意力(coordinate attention,CA)和残差连接.首先,将MixNet-s中的SE(squeeze-and-excitation)注意力替换为CA,以增强网络的信息表示能力并更精确地获取CSI图像指纹特征.其次,根据MixNet-s模型的特点构建残差连接,以增强网络的表示能力并防止过拟合.最后,通过减小网络深度确保所有网络层得到充分训练;在线阶段,采集目标设备的CSI数据并转换为图像,输入已训练好的改进MixNet模型(命名为MixNet-CA);最后利用加权质心算法根据模型输出的概率值估计目标设备的最终位置.提出方法在室内环境中进行了验证,达到了0.3620 m的平均定位误差.
基金supported by grants from the Project of Sichuan Science and Technology Department,China(Grant No.:2021YJ0010).
文摘Ginsenoside Rg5 is a rare ginsenoside showing promising tumor-suppressive effects.This study aimed to explore its radio-sensitizing effects and the underlying mechanisms.Human lung adenocarcinoma cell lines A549 and Calu-3 were used for in vitro and in vivo analysis.Bioinformatic molecular docking prediction and following validation by surface plasmon resonance(SPR)technology,cellular thermal shift assay(CETSA),and isothermal titration calorimetry(ITC)were conducted to explore the binding between ginsenoside Rg5 and 90 kD heat shock protein alpha(HSP90a).The effects of ginsenoside Rg5 on HSP90-cell division cycle 37(CDC37)interaction,the client protein stability,and the downstream regulations were further explored.Results showed that ginsenoside Rg5 could induce cell-cycle arrest at the G1 phase and enhance irradiationinduced cell apoptosis.It could bind to HSP90a with a high affinity,but the affinity was drastically decreased by HSP90a Y61A mutation.Co-immunoprecipitation(Co-IP)and ITC assays confirmed that ginsenoside Rg5 disrupts the HSP90-CDC37 interaction in a dose-dependent manner.It reduced irradiation-induced upregulation of the HSP90-CDC37 client proteins,including SRC,CDK4,RAF1,and ULK1 in A549 cell-derived xenograft(CDX)tumors.Ginsenoside Rg5 or MRT67307(an IKKε/TBK1 inhibitor)pretreatment suppressed irradiation-induced elevation of the LC3-II/b ratio and restored irradiation-induced downregulation of p62 expression.In A549 CDX tumors,ginsenoside Rg5 treatment suppressed LC3 expression and enhanced irradiation-induced DNA damage.In conclusion,ginsenoside Rg5 may be a potential radiosensitizer for lung adenocarcinoma.It interacts with HSP90a and reduces the binding between HSP90 and CDC37,thereby increasing the ubiquitin-mediated proteasomal degradation of the HSP90-CDC37 client proteins.
基金The authors are grateful for the support of the Fundamental Research Funds of Research Institute of Forest New Technology,CAF(CAFYBB2019SY039).
文摘This study aims to realize the sharing of near-infrared analysis models of lignin and holocellulose content in pulp wood on two different batches of spectrometers and proposes a combined algorithm of SPA-DS,MCUVE-DS and SiPLS-DS.The Successive Projection Algorithm(SPA),the Monte-Carlo of Uninformative Variable Elimination(MCUVE)and the Synergy Interval Partial Least Squares(SiPLS)algorithms are respectively used to reduce the adverse effects of redundant information in the transmission process of the full spectrum DS algorithm model.These three algorithms can improve model transfer accuracy and efficiency and reduce the manpower and material consumption required for modeling.These results show that the modeling effects of the characteristic wavelengths screened by the SPA,MCUVE and SiPLS algorithms are all greatly improved compared with the full-spectrum modeling,in which the SPA-PLS result in the best prediction with RPDs above 6.5 for both components.The three wavelength selection methods combined with the DS algorithm are used to transfer the models of the two instruments.Among them,the MCUVE combined with the DS algorithm has the best transfer effect.After the model transfer,the RMSEP of lignin is 0.701,and the RMSEP of holocellulose is 0.839,which was improved significantly than the full-spectrum model transfer of 0.759 and 0.918.
文摘对于温度灵敏度优于10 mK的长波红外相机,如何精确测量表征其灵敏度的指标-噪声等效温差(Noise Equivalent Temperature Difference,NETD),是一项重要的工作。首先,依照尽量缩短标准辐射传递链路减少误差项的原则,对比不同的测量方法,选取"黑体直接测量法";其次,基于相机噪声理论模型,对不同温度点下的实测噪声分析,提出噪声修正方法对噪声进行修正,以此减小环境影响带来的测量误差;最后,基于NETD测量计算公式及不确定度分析理论对测试结果的不确定度进行分析评定。结果表明:针对甚高灵敏度长波红外相机所采用的测试设备及方法,可满足测试要求,测试结果相对不确定度在7.7%左右。
